White, R J, Jackson, S P, Rigby, P W
The major class of vertebrate genes transcribed by RNA polymerase (EC 2.7.7.6) III, which includes 5S rRNA genes, tRNA genes, and the adenovirus VA genes, is characterized by split internal promoters...
The use of histone as a facilitator to improve the efficiency of retroviral gene transfer.
Vectors based on murine C-type retroviruses are commonly used in biology. The efficiency of viral infection is normally increased by a facilitator, for example polybrene, DEAE-dextran or a liposome....
Isolation of developmentally regulated genes by differential display screening of cDNA libraries.
Gupta, R, Thomas, P, Beddington, R S, Rigby, P W
mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of...
Rigby, P W, Gething, M J, Hartley, B S
Study of many of the interesting properties of Klebsiella aerogenes is limited by the lack of a well-characterized genetic system for this organism. Our investigations of the evolution of the enzyme...
The long terminal repeat (LTR) sequences of Moloney murine leukemia virus and its closely related derivative Moloney murine sarcoma virus (Mo-MSV) are incapable of directing transcription in...
Structure and synthesis of a simian virus 40 super T-antigen.
Lovett, M, Clayton, C E, Murphy, D, Rigby, P W, Smith, A E, Chaudry, F
Mouse cells transformed by simian virus 40 often contain virus-coded tumor antigens distinct from those synthesized in productively infected permissive cells. The SV3T3 C120 cell line produces no...
Functional analysis of a simian virus 40 super T-antigen.
Clayton, C E, Lovett, M, Rigby, P W
The SV3T3 C120 line of simian virus 40-transformed mouse cells synthesizes no large T-antigen of molecular weight 94,000 but instead a super T-antigen of molecular weight 145,000. In the accompanying...
The construction of cosmid libraries of eukaryotic DNA using the Homer series of vectors.
Chia, W, Scott, M R, Rigby, P W
We have constructed and characterised a series of approved, disabled cosmid vectors which we call Homer cosmids and have examined the optimal conditions for the construction of libraries of...
The structure and expression of the preproenkephalin gene.
Legon, S, Glover, D M, Hughes, J, Lowry, P J, Rigby, P W, Watson, C J
Enkephalins are pentapeptides with opioid activity which are found in a wide variety of tissues. Studies of enkephalin-containing peptides from the adrenal gland have established that the mature...
La Thangue, N B, Thimmappaya, B, Rigby, P W
Murine F9 embryonal carcinoma (EC) stem cells have an Ela-like transcription activity that is undetectable in F9 cells differentiated to parietal endoderm-like cells (F9-PE). The Ela-inducible...
Activation of endogenous retroviral transcription in SV40-transformed mouse cells.
Timmons, P M, Brickell, P M, Latchman, D S, Rigby, P W
We have previously isolated a number of cDNA clones that correspond to mRNAs present at higher levels in SV40-transformed cells than in the untransformed parental cells (Scott, M.R.D., Westphal,...
McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning.
Raleigh, E A, Murray, N E, Revel, H, Blumenthal, R M, Westaway, D, Reith, A D, ...
The McrA and McrB (modified cytosine restriction) systems of E. coli interfere with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected...
We have derived from F9 murine embryonal carcinoma stem cells, and from differentiated derivatives induced with retinoic acid and cAMP, whole cell extracts which efficiently and accurately transcribe...
Fate of viral DNA in nonpermissive cells infected with simian virus 40.
Mouse cells are nonpermissive for simian virus 40 (SV40); replication of viral DNA is undetectable and progeny virions are not produced. Infection leads instead to the establishment of stably...
Does simian virus 40 DNA integrate into cellular DNA during productive infection?
Late after infection of permissive monkey cells by simian virus 40 (SV40), large amounts of SV40 DNA (30,000 to 220,000 viral genome equivalents per cell) can be isolated with the...
Effect of passage in culture on a clone of BALB/c 3T3 cells transformed by simian virus 40.
Smith, H S, Turner, S, Leong, J A, Rigby, P W
Most simian virus 40 (SV40)-transformed BALB/c 3T3 clones employed for biochemical studies have been used without regard to passage level. To determine whether virus-induced properties are stable as...
Sequences and factors required for the F9 embryonal carcinoma stem cell E1a-like activity.
Murray, E J, Stott, D, Rigby, P W
F9 embryonal carcinoma (EC) stem cells contain an E1a-like activity that is absent from differentiated derivatives. We have previously characterized proteins present in F9 EC cell extracts that bind...
Shenk, T E, Rhodes, C, Rigby, P W, Berg, P
S1 nuclease (EC 3.1.4.X), a single-strand-specific nuclease, can be used to accurately map the location of mutational alterations in simian virus 40 (SV40) DNA. Deletions of between 32 and 190 base...
Regulation of RNA polymerase III transcription in response to Simian virus 40 transformation.
White, R J, Stott, D, Rigby, P W
Transcription by RNA polymerase III of the B2 family of middle-repetitive elements is activated in response to transformation by a variety of agents, including DNA tumour viruses, RNA tumour viruses...
Transcripts from an aberrantly re-arranged human T-cell receptor beta-chain gene.
Collins, M K, Kissonerghis, A M, Dunne, M J, Watson, C J, Rigby, P W, Owen, M J
A number of cDNA clones homologous to the human T-cell receptor beta-chain gene have been isolated from a library constructed from the human leukaemic cell line Jurkat. The nucleotide sequences of...
Cosmid clones derived from both euchromatic and heterochromatic regions of the human Y chromosome.
Wolfe, J, Erickson, R P, Rigby, P W, Goodfellow, P N
Clones containing sequences derived from the human Y chromosome have been isolated from cosmid libraries of a human-mouse hybrid cell line. These libraries were constructed in the new expression...
Gutman, A, Gilthorpe, J, Rigby, P W
Mouse Hoxb-4 (Hox-2.6) is a homeobox gene that belongs to a family which also includes Hoxa-4, Hoxc-4, and Hoxd-4 and that is related to the Deformed gene in Drosophila melanogaster. We have...
White, R J, Jackson, S P, Rigby, P W
The major class of vertebrate genes transcribed by RNA polymerase (EC 2.7.7.6) III, which includes 5S rRNA genes, tRNA genes, and the adenovirus VA genes, is characterized by split internal promoters...
The use of histone as a facilitator to improve the efficiency of retroviral gene transfer.
Vectors based on murine C-type retroviruses are commonly used in biology. The efficiency of viral infection is normally increased by a facilitator, for example polybrene, DEAE-dextran or a liposome....
Isolation of developmentally regulated genes by differential display screening of cDNA libraries.
Gupta, R, Thomas, P, Beddington, R S, Rigby, P W
mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of...
Rigby, P W, Gething, M J, Hartley, B S
Study of many of the interesting properties of Klebsiella aerogenes is limited by the lack of a well-characterized genetic system for this organism. Our investigations of the evolution of the enzyme...
The long terminal repeat (LTR) sequences of Moloney murine leukemia virus and its closely related derivative Moloney murine sarcoma virus (Mo-MSV) are incapable of directing transcription in...
Structure and synthesis of a simian virus 40 super T-antigen.
Lovett, M, Clayton, C E, Murphy, D, Rigby, P W, Smith, A E, Chaudry, F
Mouse cells transformed by simian virus 40 often contain virus-coded tumor antigens distinct from those synthesized in productively infected permissive cells. The SV3T3 C120 cell line produces no...
Functional analysis of a simian virus 40 super T-antigen.
Clayton, C E, Lovett, M, Rigby, P W
The SV3T3 C120 line of simian virus 40-transformed mouse cells synthesizes no large T-antigen of molecular weight 94,000 but instead a super T-antigen of molecular weight 145,000. In the accompanying...
The construction of cosmid libraries of eukaryotic DNA using the Homer series of vectors.
Chia, W, Scott, M R, Rigby, P W
We have constructed and characterised a series of approved, disabled cosmid vectors which we call Homer cosmids and have examined the optimal conditions for the construction of libraries of...
The structure and expression of the preproenkephalin gene.
Legon, S, Glover, D M, Hughes, J, Lowry, P J, Rigby, P W, Watson, C J
Enkephalins are pentapeptides with opioid activity which are found in a wide variety of tissues. Studies of enkephalin-containing peptides from the adrenal gland have established that the mature...
La Thangue, N B, Thimmappaya, B, Rigby, P W
Murine F9 embryonal carcinoma (EC) stem cells have an Ela-like transcription activity that is undetectable in F9 cells differentiated to parietal endoderm-like cells (F9-PE). The Ela-inducible...
Activation of endogenous retroviral transcription in SV40-transformed mouse cells.
Timmons, P M, Brickell, P M, Latchman, D S, Rigby, P W
We have previously isolated a number of cDNA clones that correspond to mRNAs present at higher levels in SV40-transformed cells than in the untransformed parental cells (Scott, M.R.D., Westphal,...
McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning.
Raleigh, E A, Murray, N E, Revel, H, Blumenthal, R M, Westaway, D, Reith, A D, ...
The McrA and McrB (modified cytosine restriction) systems of E. coli interfere with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected...
We have derived from F9 murine embryonal carcinoma stem cells, and from differentiated derivatives induced with retinoic acid and cAMP, whole cell extracts which efficiently and accurately transcribe...
Fate of viral DNA in nonpermissive cells infected with simian virus 40.
Mouse cells are nonpermissive for simian virus 40 (SV40); replication of viral DNA is undetectable and progeny virions are not produced. Infection leads instead to the establishment of stably...
Does simian virus 40 DNA integrate into cellular DNA during productive infection?
Late after infection of permissive monkey cells by simian virus 40 (SV40), large amounts of SV40 DNA (30,000 to 220,000 viral genome equivalents per cell) can be isolated with the...
Effect of passage in culture on a clone of BALB/c 3T3 cells transformed by simian virus 40.
Smith, H S, Turner, S, Leong, J A, Rigby, P W
Most simian virus 40 (SV40)-transformed BALB/c 3T3 clones employed for biochemical studies have been used without regard to passage level. To determine whether virus-induced properties are stable as...
Gutman, A, Gilthorpe, J, Rigby, P W
Mouse Hoxb-4 (Hox-2.6) is a homeobox gene that belongs to a family which also includes Hoxa-4, Hoxc-4, and Hoxd-4 and that is related to the Deformed gene in Drosophila melanogaster. We have...
Sequences and factors required for the F9 embryonal carcinoma stem cell E1a-like activity.
Murray, E J, Stott, D, Rigby, P W
F9 embryonal carcinoma (EC) stem cells contain an E1a-like activity that is absent from differentiated derivatives. We have previously characterized proteins present in F9 EC cell extracts that bind...
Shenk, T E, Rhodes, C, Rigby, P W, Berg, P
S1 nuclease (EC 3.1.4.X), a single-strand-specific nuclease, can be used to accurately map the location of mutational alterations in simian virus 40 (SV40) DNA. Deletions of between 32 and 190 base...
Regulation of RNA polymerase III transcription in response to Simian virus 40 transformation.
White, R J, Stott, D, Rigby, P W
Transcription by RNA polymerase III of the B2 family of middle-repetitive elements is activated in response to transformation by a variety of agents, including DNA tumour viruses, RNA tumour viruses...
Transcripts from an aberrantly re-arranged human T-cell receptor beta-chain gene.
Collins, M K, Kissonerghis, A M, Dunne, M J, Watson, C J, Rigby, P W, Owen, M J
A number of cDNA clones homologous to the human T-cell receptor beta-chain gene have been isolated from a library constructed from the human leukaemic cell line Jurkat. The nucleotide sequences of...
Cosmid clones derived from both euchromatic and heterochromatic regions of the human Y chromosome.
Wolfe, J, Erickson, R P, Rigby, P W, Goodfellow, P N
Clones containing sequences derived from the human Y chromosome have been isolated from cosmid libraries of a human-mouse hybrid cell line. These libraries were constructed in the new expression...