Abeijon, C, Robbins, P W, Hirschberg, C B
The mannan chains of Kluyveromyces lactis mannoproteins are similar to those of Saccharomyces cerevisiae except that they lack mannose phosphate and have terminal alpha1-->2-linked...
Semino, C E, Specht, C A, Raimondi, A, Robbins, P W
The Xenopus developmental gene DG42 is expressed during early embryonic development, between the midblastula and neurulation stages. The deduced protein sequence of Xenopus DG42 shows similarity to...
Synthesis of "Nod"-like chitin oligosaccharides by the Xenopus developmental protein DG42.
The Xenopus DG42 gene is expressed only between the late midblastula and neurulation stages of embryonic development. Recent database searches show that DG42 has striking sequence similarity to the...
Molecular cloning and characterization of chitinase genes from Candida albicans.
McCreath, K J, Specht, C A, Robbins, P W
Chitinase (EC 3.2.1.14) is an important enzyme for the remodeling of chitin in the cell wall of fungi. We have cloned three chitinase genes (CHT1, CHT2, and CHT3) from the dimorphic human pathogen...
Cloning and analysis of murine cDNA that encodes a fibrogenic lymphokine, fibrosin.
Prakash, S, Robbins, P W, Wyler, D J
Tissue fibrosis that complicates chronic inflammation can be a cause of serious morbidity. The molecular links between inflammation and fibrosis appear to be a variety of proteins produced by...
Gow, N A, Robbins, P W, Lester, J W, Brown, A J, Fonzi, W A, Chapman, T, ...
In the dimorphic fungus Candida albicans, the CHS2 gene encodes a chitin synthase that is expressed preferentially in the hyphal form. Gene disruption of CHS2 in this diploid asexual fungus was...
Classification of fungal chitin synthases.
Bowen, A R, Chen-Wu, J L, Momany, M, Young, R, Szaniszlo, P J, Robbins, P W
Comparison of the chitin synthase genes of Saccharomyces cerevisiae CHS1 and CHS2 with the Candida albicans CHS1 gene (UDP-N-acetyl-D-glucosamine:chitin 4-beta-N-acetylglucosaminyltransferase, EC...
Häusler, A, Ballou, L, Ballou, C E, Robbins, P W
The Saccharomyces cerevisiae MNT1 gene encodes a Golgi mannosyltransferase. Gene disruption of the MNT1 locus leads to a greater than 90% reduction of specific alpha-1,2-mannosyltransferase activity...
Lu, M C, Lien, M H, Becker, R E, Heine, H C, Buggs, A M, Lipovsek, D, ...
The relatedness of immunodominant protein antigens in Mycobacterium tuberculosis, M. africanum, and M. bovis BCG was investigated by comparing the genes that encode major protein antigens in M....
BIOSYNTHESIS OF CHLORAMPHENICOL IV. : Incorporation of Carbon14-Labeled Precursors
Gottlieb, David, Carter, H. E., Robbins, P. W., Burg, R. W.
Gottlieb, David (University of Illinois, Urbana), H. E. Carter, P. W. Robbins, and R. W. Burg. Biosynthesis of chloramphenicol. IV. Incorporation of carbon14-labeled precursors. J. Bacteriol....
Abeijon, C, Orlean, P, Robbins, P W, Hirschberg, C B
"Outer-chain" addition of mannose residues to yeast glycoproteins occurs in the Golgi compartment of the cell. Essential steps in this process are thought to include transport of GDPmannose from the...
A 13-amino acid peptide in three yeast glycosyltransferases may be involved in dolichol recognition.
Albright, C F, Orlean, P, Robbins, P W
A 13-amino acid peptide was identified in three glycosyltransferases of the yeast endoplasmic reticulum. These enzymes, the products of the ALG1, ALG7, and DPM1 genes, catalyze the transfer of sugars...
Protein glycosylation in yeast: transcript heterogeneity of the ALG7 gene.
Kukuruzinska, M A, Robbins, P W
The first enzyme in the lipid-linked oligosaccharide biosynthetic pathway, UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase...
Cloning and heterologous expression of glycosidase genes from Saccharomyces cerevisiae.
Genomic clones were isolated that code for three glycosidases proposed to be involved in the catabolism of cell wall components in Saccharomyces cerevisiae. alpha-Mannosidase (AMS1), exoglucanase...
Bergh, M L, Cepko, C L, Wolf, D, Robbins, P W
Oligosaccharide processing is controlled by host- and protein-dependent factors. To increase our understanding of the relative contribution of those factors we studied the glycosylation of yeast...
In an effort to understand the factors that influence protein glycosylation, we are studying the expression of the chicken ovalbumin gene in a heterologous cell. Ovalbumin synthesized in mouse L-353...
Relationship of GIX antigen expression to the glycosylation of murine leukemia virus glycoprotein.
Rosner, M R, Tung, J S, Hopkins, N, Robbins, P W
The GIX antigen, which is expressed on the surface of thymocytes of certain mouse strains, is an antigenic determinant of the major envelope glycoprotein of murine leukemia virus (gp70). Although GIX...
Beck, P J, Orlean, P, Albright, C, Robbins, P W, Gething, M J, Sambrook, J F
The Saccharomyces cerevisiae DPM1 gene product, dolichol-phosphate-mannose (Dol-P-Man) synthase, is involved in the coupled processes of synthesis and membrane translocation of Dol-P-Man. Dol-P-Man...
Yeast mutants deficient in protein glycosylation.
The synthesis of asparagine-linked oligosaccharides involves the formation of a lipid-linked precursor oligosaccharide that has the composition Glc3Man9GlcNAc2. We have used a [3H]mannose suicide...
Turco, S J, Stetson, B, Robbins, P W
We have shown previously that particulate preparations of Nil 8 fibroblasts catalyze the synthesis of two oligosaccharide-lipids: one composed of N-acetylglucosamine and mannose residues and the...
Decrease in membrane-associated actin of fibroblasts after transformation by Rous sarcoma virus.
Wickus, G, Gruenstein, E, Robbins, P W, Rich, A
The actin content of membranes prepared from cultured chick embryo fibroblasts has been measured on polyacrylamide gels. The actin was identified by tryptic peptide mapping. After transformation of...
Fluorescence polarization and viscosities of membrane lipids of 3T3 cells.
Fuchs, P, Parola, A, Robbins, P W, Blout, E R
Studies of the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene in membranes of normal, transformed, and revertant 3T3 cells allowed estimation of microviscosities (eta) of the lipid...
Lussier, M., White, A. M., Sheraton, J., Di-Paolo, T., Treadwell, J., Southard, S. B., ...
The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes...
Abeijon, C, Robbins, P W, Hirschberg, C B
The mannan chains of Kluyveromyces lactis mannoproteins are similar to those of Saccharomyces cerevisiae except that they lack mannose phosphate and have terminal alpha1-->2-linked...
Semino, C E, Specht, C A, Raimondi, A, Robbins, P W
The Xenopus developmental gene DG42 is expressed during early embryonic development, between the midblastula and neurulation stages. The deduced protein sequence of Xenopus DG42 shows similarity to...
Synthesis of "Nod"-like chitin oligosaccharides by the Xenopus developmental protein DG42.
The Xenopus DG42 gene is expressed only between the late midblastula and neurulation stages of embryonic development. Recent database searches show that DG42 has striking sequence similarity to the...
Molecular cloning and characterization of chitinase genes from Candida albicans.
McCreath, K J, Specht, C A, Robbins, P W
Chitinase (EC 3.2.1.14) is an important enzyme for the remodeling of chitin in the cell wall of fungi. We have cloned three chitinase genes (CHT1, CHT2, and CHT3) from the dimorphic human pathogen...
Cloning and analysis of murine cDNA that encodes a fibrogenic lymphokine, fibrosin.
Prakash, S, Robbins, P W, Wyler, D J
Tissue fibrosis that complicates chronic inflammation can be a cause of serious morbidity. The molecular links between inflammation and fibrosis appear to be a variety of proteins produced by...
Gow, N A, Robbins, P W, Lester, J W, Brown, A J, Fonzi, W A, Chapman, T, ...
In the dimorphic fungus Candida albicans, the CHS2 gene encodes a chitin synthase that is expressed preferentially in the hyphal form. Gene disruption of CHS2 in this diploid asexual fungus was...
Classification of fungal chitin synthases.
Bowen, A R, Chen-Wu, J L, Momany, M, Young, R, Szaniszlo, P J, Robbins, P W
Comparison of the chitin synthase genes of Saccharomyces cerevisiae CHS1 and CHS2 with the Candida albicans CHS1 gene (UDP-N-acetyl-D-glucosamine:chitin 4-beta-N-acetylglucosaminyltransferase, EC...
Häusler, A, Ballou, L, Ballou, C E, Robbins, P W
The Saccharomyces cerevisiae MNT1 gene encodes a Golgi mannosyltransferase. Gene disruption of the MNT1 locus leads to a greater than 90% reduction of specific alpha-1,2-mannosyltransferase activity...
Lu, M C, Lien, M H, Becker, R E, Heine, H C, Buggs, A M, Lipovsek, D, ...
The relatedness of immunodominant protein antigens in Mycobacterium tuberculosis, M. africanum, and M. bovis BCG was investigated by comparing the genes that encode major protein antigens in M....
BIOSYNTHESIS OF CHLORAMPHENICOL IV. : Incorporation of Carbon14-Labeled Precursors
Gottlieb, David, Carter, H. E., Robbins, P. W., Burg, R. W.
Gottlieb, David (University of Illinois, Urbana), H. E. Carter, P. W. Robbins, and R. W. Burg. Biosynthesis of chloramphenicol. IV. Incorporation of carbon14-labeled precursors. J. Bacteriol....
Abeijon, C, Orlean, P, Robbins, P W, Hirschberg, C B
"Outer-chain" addition of mannose residues to yeast glycoproteins occurs in the Golgi compartment of the cell. Essential steps in this process are thought to include transport of GDPmannose from the...
A 13-amino acid peptide in three yeast glycosyltransferases may be involved in dolichol recognition.
Albright, C F, Orlean, P, Robbins, P W
A 13-amino acid peptide was identified in three glycosyltransferases of the yeast endoplasmic reticulum. These enzymes, the products of the ALG1, ALG7, and DPM1 genes, catalyze the transfer of sugars...
Protein glycosylation in yeast: transcript heterogeneity of the ALG7 gene.
Kukuruzinska, M A, Robbins, P W
The first enzyme in the lipid-linked oligosaccharide biosynthetic pathway, UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase...
Cloning and heterologous expression of glycosidase genes from Saccharomyces cerevisiae.
Genomic clones were isolated that code for three glycosidases proposed to be involved in the catabolism of cell wall components in Saccharomyces cerevisiae. alpha-Mannosidase (AMS1), exoglucanase...
Bergh, M L, Cepko, C L, Wolf, D, Robbins, P W
Oligosaccharide processing is controlled by host- and protein-dependent factors. To increase our understanding of the relative contribution of those factors we studied the glycosylation of yeast...
In an effort to understand the factors that influence protein glycosylation, we are studying the expression of the chicken ovalbumin gene in a heterologous cell. Ovalbumin synthesized in mouse L-353...
Relationship of GIX antigen expression to the glycosylation of murine leukemia virus glycoprotein.
Rosner, M R, Tung, J S, Hopkins, N, Robbins, P W
The GIX antigen, which is expressed on the surface of thymocytes of certain mouse strains, is an antigenic determinant of the major envelope glycoprotein of murine leukemia virus (gp70). Although GIX...
Beck, P J, Orlean, P, Albright, C, Robbins, P W, Gething, M J, Sambrook, J F
The Saccharomyces cerevisiae DPM1 gene product, dolichol-phosphate-mannose (Dol-P-Man) synthase, is involved in the coupled processes of synthesis and membrane translocation of Dol-P-Man. Dol-P-Man...
Yeast mutants deficient in protein glycosylation.
The synthesis of asparagine-linked oligosaccharides involves the formation of a lipid-linked precursor oligosaccharide that has the composition Glc3Man9GlcNAc2. We have used a [3H]mannose suicide...
Turco, S J, Stetson, B, Robbins, P W
We have shown previously that particulate preparations of Nil 8 fibroblasts catalyze the synthesis of two oligosaccharide-lipids: one composed of N-acetylglucosamine and mannose residues and the...
Decrease in membrane-associated actin of fibroblasts after transformation by Rous sarcoma virus.
Wickus, G, Gruenstein, E, Robbins, P W, Rich, A
The actin content of membranes prepared from cultured chick embryo fibroblasts has been measured on polyacrylamide gels. The actin was identified by tryptic peptide mapping. After transformation of...
Fluorescence polarization and viscosities of membrane lipids of 3T3 cells.
Fuchs, P, Parola, A, Robbins, P W, Blout, E R
Studies of the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene in membranes of normal, transformed, and revertant 3T3 cells allowed estimation of microviscosities (eta) of the lipid...
Lussier, M., White, A. M., Sheraton, J., Di-Paolo, T., Treadwell, J., Southard, S. B., ...
The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes...
Robbins, P. W., Wright, A., Dankert, M.
Polysaccharide synthesis is discussed from the point of view of the sources of biological information that determine the structures and control the rates of synthesis of complex polysaccharides. It...
CELL MITOTIC CYCLE SYNTHESIS OF NIL HAMSTER GLYCOLIPIDS INCLUDING THE FORSSMAN ANTIGEN
Wolf, Barbara Anne, Robbins, P. W.
The synthesis of phospholipids and glycolipids during the cell mitotic cycle of an established hamster line, NIL, has been studied. Cells were synchronized with excess thymidine and mitotically...