The BCL11 gene family: involvement of BCL11A in lymphoid malignancies (2001)
Satterwhite, E., Sonoki, T., Willis, T.G., Harder, L., Nowak, R., Arriola, E.L., ...
ARID proteins come in from the desert (2000)
Kortschak, Robert Daniel, Tucker, P. W., Saint, Robert Bryce
Copyright © 2000 Elsevier Science Ltd. All rights reserved.
ARID proteins come in from the desert (2000)
Kortschak, Robert Daniel, Tucker, P. W., Saint, Robert Bryce
Copyright © 2000 Elsevier Science Ltd. All rights reserved.
Ariizumi, K, Wang, Z, Tucker, P W
In several animal viruses, enhancers have been implicated in both DNA replication and transcriptional activation. The linkage of the two mechanisms appears intimate, in that common DNA binding...
Tuaillon, N, Taylor, L D, Lonberg, N, Tucker, P W, Capra, J D
We (N.L. and L.D.T.) have introduced a human heavy-chain minilocus into mice transgenically. Constructs contain 2 heavy-chain variable (VH; psi VH3-105 and VH5-251), 10 diversity (D), 6 heavy-chain...
An 84-amino acid segment of QRF-1 [glutamine (Q)-rich factor 1], a newly cloned, B-cell-derived DNA-binding protein, shows significant sequence homology with the DNA-binding domains of the hepatocyte...
Recombinant antibody-metallothionein: design and evaluation for radioimmunoimaging.
Das, C, Kulkarni, P V, Constantinescu, A, Antich, P, Blattner, F R, Tucker, P W
We have produced a chimeric antibody (Ab) in which metallothionein, a well-characterized biological chelator of metals, was genetically fused to the F(ab') domain of the S107 Ab heavy chain....
Metal-binding chimeric antibodies expressed in Escherichia coli.
Sawyer, J R, Tucker, P W, Blattner, F R
Metallothionein, a well-characterized biological chelator of metals, has been genetically fused to the binding domain of an antibody and expressed in the periplasm of Escherichia coli. Specific...
NonO enhances the association of many DNA-binding proteins to their targets.
Yang, Y S, Yang, M C, Tucker, P W, Capra, J D
NonO is an unusual nucleic acid binding protein not only in that it binds both DNA and RNA but that it does so via functionally separable domains. Here we document that NonO enhances the binding of...
Galli, G, Guise, J, Tucker, P W, Nevins, J R
Alternative processing of the immunoglobulin mu primary transcript results in regulated production of mRNAs encoding the secreted (microseconds) and membrane-bound (micro m) form of IgM heavy chain...
Hanke, J H, Landolfi, N F, Tucker, P W, Capra, J D
Sequence-specific DNA-affinity chromatography was used to purify a nuclear protein from the B-cell leukemia cell line BCL1 that specifically binds to the octamer sequence ATTTGCAT, previously shown...
Immunoglobulin receptor signaling depends on the carboxyl terminus but not the heavy-chain class.
Webb, C F, Nakai, C, Tucker, P W
To examine the isotypic and structural requirements involved in signaling through the immunoglobulin (Ig) receptor on B lymphocytes, we established a panel of T15 idiotype-positive transfectants that...
Protein-nucleotide contacts in the immunoglobulin heavy-chain promoter region.
Landolfi, N F, Capra, J D, Tucker, P W
Immunoglobulin heavy-chain variable-region genes contain the octanucleotide ATGCAAAT upstream from the site of transcription initiation. The complement of this sequence, in the reverse orientation,...
Production and characterization of recombinant human Ku antigen.
Ono, M, Tucker, P W, Capra, J D
Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an...
We have developed a strategy for DNA sequencing based on exonuclease III digestion followed by double strand specific endonuclease digestion and direct dideoxynucleotide sequencing reaction. This...
Regulatory elements necessary for termination of transcription within the Ig heavy chain gene locus.
Moore, B B, Tan, J, Lim, P L, Tucker, P W, Yuan, D
Previous experiments have shown that the extent of delta gene transcription during B cell development is regulated primarily at the transcriptional level. We have shown that deletion of a sequence...
Construction of recombinant DNA by exonuclease recession.
Yang, Y S, Watson, W J, Tucker, P W, Capra, J D
We describe a new exonuclease-based method for joining and/or constructing two or more DNA molecules. DNA fragments containing ends complementary to those of a vector or another independent molecules...
McGuire, K L, Duncan, W R, Tucker, P W
We have analyzed the JH-Cmu locus of the Syrian hamster by DNA cloning and sequencing. The single Cmu gene is highly homologous to that of the mouse. The hamster equivalents of the JH and switch (S)...
Landolfi, N F, Yin, X M, Capra, J D, Tucker, P W
Immunoglobulin genes contain a conserved eight base sequence element 5' to the site of transcription initiation. This octamer can serve as a site for the binding of nuclear proteins which are...
Gilliam, A C, Shen, A, Richards, J E, Blattner, F R, Mushinski, J F, Tucker, P W
We present here the sequence characterization of a C mu----C delta immunoglobulin (Ig) heavy chain class switch. In the murine IgD-secreting plasmacytoma TEPC 1017, deletion of most of the mu switch...
Knapp, M R, Liu, C P, Newell, N, Ward, R B, Tucker, P W, Strober, S, ...
The cloned murine B-cell lymphoma line (BCL1) that expresses surface IgM and IgD is considered to be a model for the immunoglobulin gene expression of the mature virgin B cell. Of particular interest...
Robinson, R A, Tucker, P W, Dauenhauer, S A, O'Callaghan, D J
Genomic DNA sequences of equine herpesvirus type 1 (EHV-1) have been cloned as BamHI and EcoRI restriction fragments into the plasmid pBR322 and propagated in Escherichia coli. With the exception of...
Mouse IgA heavy chain gene sequence: implications for evolution of immunoglobulin hinge axons.
Tucker, P W, Slightom, J L, Blattner, F R
The complete nucleotide sequence of the gene and mRNA coding for the constant (C) region of the secreted form of the BALB/c mouse IgA immunoglobulin alpha heavy (H) chain has been determined. As in...
Mouse immunoglobulin D: construction and characterization of a cloned delta chain cDNA.
Mushinski, J F, Blattner, F R, Owens, J D, Finkelman, F D, Kessler, S W, Fitzmaurice, L, ...
TEPC 1017 is a BALB/c plasmacytoma that synthesizes IgD in large enough amounts to permit the isolation of mRNA for mouse delta chains. cDNA has been prepared from this mRNA, and an 880-base-pair...
Functional analysis of the V gamma 3 promoter of the murine gamma delta T-cell receptor.
The initial day 14 wave of fetal thymocytes express a gamma delta T-cell receptor (TCR). This surface TCR is generated by preferential rearrangement of V gamma 3 and V delta 1 recombination segments....
Yang, Y S, Hanke, J H, Carayannopoulos, L, Craft, C M, Capra, J D, Tucker, P W
We have cloned the ubiquitous form of an octamer-binding, 60-kDa protein (NonO) that appears to be the mammalian equivalent of the Drosophila visual and courtship song behavior protein,...
Ariizumi, K, Ghosh, M R, Tucker, P W
In a previous study, we showed that the immunoglobulin heavy-chain (IgH) enhancer (IgHe) is near or in an initiation zone of chromosomal DNA replication, which is preferentially active in B cells (K....
Immunoglobulin D switching can occur through homologous recombination in human B cells.
White, M B, Word, C J, Humphries, C G, Blattner, F R, Tucker, P W
Prototypical class switching in mouse and human immunoglobulin heavy chains occurs through recombination of tandem blocks of short repeats located 5' to each heavy chain constant region (CH) except C...
The ubiquitous octamer-binding protein(s) is sufficient for transcription of immunoglobulin genes.
Johnson, D G, Carayannopoulos, L, Capra, J D, Tucker, P W, Hanke, J H
All immunoglobulin genes contain a conserved octanucleotide promoter element, ATGCAAAT, which has been shown to be required for their normal B-cell-specific transcription. Proteins that bind this...
Webb, C F, Das, C, Eaton, S, Calame, K, Tucker, P W
Although much has been learned about basal levels of immunoglobulin (Ig) transcription, the regulatory effects of cytokines and antigen (Ag) upon Ig expression in lymphocytes have not been fully...
Webb, C F, Das, C, Eneff, K L, Tucker, P W
In the accompanying report (C. F. Webb, C. Das, S. Eaton, K. Calame, and P. Tucker, Mol. Cell. Biol. 11:5197-5205, 1991), we characterize B-cell-specific protein-DNA interactions at -500 and -200 bp...
Tucker, P W, Phillips, S E, Perutz, M F, Houtchens, R, Caughey, W S
In hemoglobin Zürich the side chain of the distal arginine attaches itself to the propionate of the heme, leaving the heme pocket wide open, allowing sulfanilamides easy access to the iron, and...
Cotton, F A, Gillen, R G, Gohil, R N, Hazen, E E, Kirchner, C R, Nagyvary, J, ...
The P-O-ethyl ester of cAMP has been synthesized, its inhibition of solid and ascites tumors studied, and its pattern of urinary excretion followed. Et-cAMP is more effective than cAMP against solid...
Organization of the murine immunoglobulin VH complex in the inbred strains.
Rathbun, G A, Capra, J D, Tucker, P W
Deletion mapping analyses have been employed to order the heavy chain variable region (VH) gene families in three inbred murine strains. These nine VH gene families have been positioned with respect...
The murine immunoglobulin alpha gene expresses multiple transcripts from a unique membrane exon.
Word, C J, Mushinski, J F, Tucker, P W
Immunoglobulin heavy chains of each class exist in two forms, membrane (m) and secreted (s), which have different carboxy-terminal sequences encoded by different gene segments. We have sequenced the...
Structural analysis of the murine IgG3 constant region gene.
Wels, J A, Word, C J, Rimm, D, Der-Balan, G P, Martinez, H M, Tucker, P W, ...
We have determined the complete sequence of the gamma 3 heavy chain constant (C gamma 3) region gene of the BALB/c mouse including the 5'-flanking region up to the switch site and the 3'-flanking...
Ariizumi, K, Wang, Z, Tucker, P W
In several animal viruses, enhancers have been implicated in both DNA replication and transcriptional activation. The linkage of the two mechanisms appears intimate, in that common DNA binding...
Tuaillon, N, Taylor, L D, Lonberg, N, Tucker, P W, Capra, J D
We (N.L. and L.D.T.) have introduced a human heavy-chain minilocus into mice transgenically. Constructs contain 2 heavy-chain variable (VH; psi VH3-105 and VH5-251), 10 diversity (D), 6 heavy-chain...
An 84-amino acid segment of QRF-1 [glutamine (Q)-rich factor 1], a newly cloned, B-cell-derived DNA-binding protein, shows significant sequence homology with the DNA-binding domains of the hepatocyte...
Recombinant antibody-metallothionein: design and evaluation for radioimmunoimaging.
Das, C, Kulkarni, P V, Constantinescu, A, Antich, P, Blattner, F R, Tucker, P W
We have produced a chimeric antibody (Ab) in which metallothionein, a well-characterized biological chelator of metals, was genetically fused to the F(ab') domain of the S107 Ab heavy chain....
Metal-binding chimeric antibodies expressed in Escherichia coli.
Sawyer, J R, Tucker, P W, Blattner, F R
Metallothionein, a well-characterized biological chelator of metals, has been genetically fused to the binding domain of an antibody and expressed in the periplasm of Escherichia coli. Specific...
NonO enhances the association of many DNA-binding proteins to their targets.
Yang, Y S, Yang, M C, Tucker, P W, Capra, J D
NonO is an unusual nucleic acid binding protein not only in that it binds both DNA and RNA but that it does so via functionally separable domains. Here we document that NonO enhances the binding of...
Galli, G, Guise, J, Tucker, P W, Nevins, J R
Alternative processing of the immunoglobulin mu primary transcript results in regulated production of mRNAs encoding the secreted (microseconds) and membrane-bound (micro m) form of IgM heavy chain...
Hanke, J H, Landolfi, N F, Tucker, P W, Capra, J D
Sequence-specific DNA-affinity chromatography was used to purify a nuclear protein from the B-cell leukemia cell line BCL1 that specifically binds to the octamer sequence ATTTGCAT, previously shown...
Immunoglobulin receptor signaling depends on the carboxyl terminus but not the heavy-chain class.
Webb, C F, Nakai, C, Tucker, P W
To examine the isotypic and structural requirements involved in signaling through the immunoglobulin (Ig) receptor on B lymphocytes, we established a panel of T15 idiotype-positive transfectants that...
Protein-nucleotide contacts in the immunoglobulin heavy-chain promoter region.
Landolfi, N F, Capra, J D, Tucker, P W
Immunoglobulin heavy-chain variable-region genes contain the octanucleotide ATGCAAAT upstream from the site of transcription initiation. The complement of this sequence, in the reverse orientation,...
Production and characterization of recombinant human Ku antigen.
Ono, M, Tucker, P W, Capra, J D
Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an...
We have developed a strategy for DNA sequencing based on exonuclease III digestion followed by double strand specific endonuclease digestion and direct dideoxynucleotide sequencing reaction. This...
Regulatory elements necessary for termination of transcription within the Ig heavy chain gene locus.
Moore, B B, Tan, J, Lim, P L, Tucker, P W, Yuan, D
Previous experiments have shown that the extent of delta gene transcription during B cell development is regulated primarily at the transcriptional level. We have shown that deletion of a sequence...
Construction of recombinant DNA by exonuclease recession.
Yang, Y S, Watson, W J, Tucker, P W, Capra, J D
We describe a new exonuclease-based method for joining and/or constructing two or more DNA molecules. DNA fragments containing ends complementary to those of a vector or another independent molecules...
McGuire, K L, Duncan, W R, Tucker, P W
We have analyzed the JH-Cmu locus of the Syrian hamster by DNA cloning and sequencing. The single Cmu gene is highly homologous to that of the mouse. The hamster equivalents of the JH and switch (S)...
Landolfi, N F, Yin, X M, Capra, J D, Tucker, P W
Immunoglobulin genes contain a conserved eight base sequence element 5' to the site of transcription initiation. This octamer can serve as a site for the binding of nuclear proteins which are...
Gilliam, A C, Shen, A, Richards, J E, Blattner, F R, Mushinski, J F, Tucker, P W
We present here the sequence characterization of a C mu----C delta immunoglobulin (Ig) heavy chain class switch. In the murine IgD-secreting plasmacytoma TEPC 1017, deletion of most of the mu switch...
Knapp, M R, Liu, C P, Newell, N, Ward, R B, Tucker, P W, Strober, S, ...
The cloned murine B-cell lymphoma line (BCL1) that expresses surface IgM and IgD is considered to be a model for the immunoglobulin gene expression of the mature virgin B cell. Of particular interest...
Robinson, R A, Tucker, P W, Dauenhauer, S A, O'Callaghan, D J
Genomic DNA sequences of equine herpesvirus type 1 (EHV-1) have been cloned as BamHI and EcoRI restriction fragments into the plasmid pBR322 and propagated in Escherichia coli. With the exception of...
Mouse IgA heavy chain gene sequence: implications for evolution of immunoglobulin hinge axons.
Tucker, P W, Slightom, J L, Blattner, F R
The complete nucleotide sequence of the gene and mRNA coding for the constant (C) region of the secreted form of the BALB/c mouse IgA immunoglobulin alpha heavy (H) chain has been determined. As in...
Mouse immunoglobulin D: construction and characterization of a cloned delta chain cDNA.
Mushinski, J F, Blattner, F R, Owens, J D, Finkelman, F D, Kessler, S W, Fitzmaurice, L, ...
TEPC 1017 is a BALB/c plasmacytoma that synthesizes IgD in large enough amounts to permit the isolation of mRNA for mouse delta chains. cDNA has been prepared from this mRNA, and an 880-base-pair...
Functional analysis of the V gamma 3 promoter of the murine gamma delta T-cell receptor.
The initial day 14 wave of fetal thymocytes express a gamma delta T-cell receptor (TCR). This surface TCR is generated by preferential rearrangement of V gamma 3 and V delta 1 recombination segments....
Yang, Y S, Hanke, J H, Carayannopoulos, L, Craft, C M, Capra, J D, Tucker, P W
We have cloned the ubiquitous form of an octamer-binding, 60-kDa protein (NonO) that appears to be the mammalian equivalent of the Drosophila visual and courtship song behavior protein,...
Ariizumi, K, Ghosh, M R, Tucker, P W
In a previous study, we showed that the immunoglobulin heavy-chain (IgH) enhancer (IgHe) is near or in an initiation zone of chromosomal DNA replication, which is preferentially active in B cells (K....
Immunoglobulin D switching can occur through homologous recombination in human B cells.
White, M B, Word, C J, Humphries, C G, Blattner, F R, Tucker, P W
Prototypical class switching in mouse and human immunoglobulin heavy chains occurs through recombination of tandem blocks of short repeats located 5' to each heavy chain constant region (CH) except C...
The ubiquitous octamer-binding protein(s) is sufficient for transcription of immunoglobulin genes.
Johnson, D G, Carayannopoulos, L, Capra, J D, Tucker, P W, Hanke, J H
All immunoglobulin genes contain a conserved octanucleotide promoter element, ATGCAAAT, which has been shown to be required for their normal B-cell-specific transcription. Proteins that bind this...
Webb, C F, Das, C, Eaton, S, Calame, K, Tucker, P W
Although much has been learned about basal levels of immunoglobulin (Ig) transcription, the regulatory effects of cytokines and antigen (Ag) upon Ig expression in lymphocytes have not been fully...
Webb, C F, Das, C, Eneff, K L, Tucker, P W
In the accompanying report (C. F. Webb, C. Das, S. Eaton, K. Calame, and P. Tucker, Mol. Cell. Biol. 11:5197-5205, 1991), we characterize B-cell-specific protein-DNA interactions at -500 and -200 bp...
Tucker, P W, Phillips, S E, Perutz, M F, Houtchens, R, Caughey, W S
In hemoglobin Zürich the side chain of the distal arginine attaches itself to the propionate of the heme, leaving the heme pocket wide open, allowing sulfanilamides easy access to the iron, and...
Cotton, F A, Gillen, R G, Gohil, R N, Hazen, E E, Kirchner, C R, Nagyvary, J, ...
The P-O-ethyl ester of cAMP has been synthesized, its inhibition of solid and ascites tumors studied, and its pattern of urinary excretion followed. Et-cAMP is more effective than cAMP against solid...
Organization of the murine immunoglobulin VH complex in the inbred strains.
Rathbun, G A, Capra, J D, Tucker, P W
Deletion mapping analyses have been employed to order the heavy chain variable region (VH) gene families in three inbred murine strains. These nine VH gene families have been positioned with respect...
The murine immunoglobulin alpha gene expresses multiple transcripts from a unique membrane exon.
Word, C J, Mushinski, J F, Tucker, P W
Immunoglobulin heavy chains of each class exist in two forms, membrane (m) and secreted (s), which have different carboxy-terminal sequences encoded by different gene segments. We have sequenced the...