P. Wingfield

Characterization of recombinant-derived granulocyte-colony stimulating factor (G-CSF) (2006)

Wingfield, P., Benedict, R., Turcatti, G., Allet, B., Mermod, J. J., DeLamarter, J., ...

Human granulocyte colony-stimulating factor (G-CSF), and a mutant having a Ser for Cys substitution at residue 18 were produced in Escherichia coli strain W3110. About 60 mg of pure protein was...

Purification and characterization of a methionine-specific aminopeptidase from Salmonella typhimurium (2006)

Wingfield, P., Graber, P., Turcatti, G., Movva, N. R., Pelletier, M., Craig, S., ...

An aminopeptidase specific for methionine (peptidase M) has been purified from wild-type and mutant Salmonella typhimurium strains. Recombinant peptidase M was also purified from Escherichia coli....

Structure and partial amino acid sequence of calf thymus DNA topoisomerase II: Comparison with other type II enzymes (2006)

Austin, C. A., Barot, H. A., Margerrison, E. E. C., Turcatti, G., Wingfield, P., Hayes, M. V., ...

The partial amino acid sequence of p140 calf thymus DNA topoisomerase II was determined by analysis of cyanogen bromide peptides. Five peptides were aligned and shared extensive homology with...

Tumor necrosis factor inhibitor: purification, NH2-terminal amino acid sequence and evidence for anti-inflammatory and immunomodulatory activities (2006)

Seckinger, P., Vey, E., Turcatti, G., Wingfield, P., Dayer, J. M.

The urine of some febrile patients has been shown to contain a tumor necrosis factor-alpha-inhibiting activity (TNF-alpha INH) when tested in a cytotoxicity assay using the TNF-susceptible cell line...

Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding.

Bushman, F D, Engelman, A, Palmer, I, Wingfield, P, Craigie, R

The integrase protein of human immunodeficiency virus type 1 carries out a set of polynucleotidyl transfer reactions that result in the covalent attachment of the retroviral cDNA to host DNA. We have...

Release, uptake, and effects of extracellular human immunodeficiency virus type 1 Tat protein on cell growth and viral transactivation.

Ensoli, B, Buonaguro, L, Barillari, G, Fiorelli, V, Gendelman, R, Morgan, R A, ...

During acute human immunodeficiency virus type 1 (HIV-1) infection or after transfection of the tat gene, Tat protein is released into the cell culture supernatant. In this extracellular form, Tat...

Human immunodeficiency virus rev protein recognizes a target sequence in rev-responsive element RNA within the context of RNA secondary structure.

Holland, S M, Ahmad, N, Maitra, R K, Wingfield, P, Venkatesan, S

Human immunodeficiency virus type 1 Rev protein modulates the distribution of viral mRNAs from the nucleus to the cytoplasm by interaction with a highly structured viral RNA sequence, the...

Specific complex of human immunodeficiency virus type 1 rev and nucleolar B23 proteins: dissociation by the Rev response element.

Fankhauser, C, Izaurralde, E, Adachi, Y, Wingfield, P, Laemmli, U K

The human immunodeficiency virus type 1 (HIV) Rev protein is thought to be involved in the export of unspliced or singly spliced viral mRNAs from the nucleus to the cytoplasm. This function is...

Hepatitis B virus antigens made in microbial cells immunise against viral infection.

Murray, K, Bruce, S A, Hinnen, A, Wingfield, P, Van Erd, P M, De Reus, A, ...

Chimpanzees have been vaccinated successfully against hepatitis B virus with preparations of the viral antigens made in microbial cells by genetic engineering methods.

Characterization of recombinant-derived granulocyte-colony stimulating factor (G-CSF).

Wingfield, P, Benedict, R, Turcatti, G, Allet, B, Mermod, J J, DeLamarter, J, ...

Human granulocyte colony-stimulating factor (G-CSF), and a mutant having a Ser for Cys substitution at residue 18 were produced in Escherichia coli strain W3110. About 60 mg of pure protein was...

Characterization of human and mouse granulocyte-macrophage-colony-stimulating factors derived from Escherichia coli.

Schrimsher, J L, Rose, K, Simona, M G, Wingfield, P

Human and mouse granulocyte-macrophage-colony-stimulating factors (hGM-CSF and mGM-CSF, respectively), isolated from Escherichia coli cells expressing the corresponding human and mouse genes, have...

C-terminal peptide identification by fast atom bombardment mass spectrometry.

Rose, K, Savoy, L A, Simona, M G, Offord, R E, Wingfield, P

A previously described technique [Rose, Simona, Offord, Prior, Otto & Thatcher (1983) Biochem. J. 215, 273-277] permits the identification of the C-terminal peptide of a protein as the only peptide...

Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding.

Bushman, F D, Engelman, A, Palmer, I, Wingfield, P, Craigie, R

The integrase protein of human immunodeficiency virus type 1 carries out a set of polynucleotidyl transfer reactions that result in the covalent attachment of the retroviral cDNA to host DNA. We have...

Release, uptake, and effects of extracellular human immunodeficiency virus type 1 Tat protein on cell growth and viral transactivation.

Ensoli, B, Buonaguro, L, Barillari, G, Fiorelli, V, Gendelman, R, Morgan, R A, ...

During acute human immunodeficiency virus type 1 (HIV-1) infection or after transfection of the tat gene, Tat protein is released into the cell culture supernatant. In this extracellular form, Tat...

Human immunodeficiency virus rev protein recognizes a target sequence in rev-responsive element RNA within the context of RNA secondary structure.

Holland, S M, Ahmad, N, Maitra, R K, Wingfield, P, Venkatesan, S

Human immunodeficiency virus type 1 Rev protein modulates the distribution of viral mRNAs from the nucleus to the cytoplasm by interaction with a highly structured viral RNA sequence, the...

Specific complex of human immunodeficiency virus type 1 rev and nucleolar B23 proteins: dissociation by the Rev response element.

Fankhauser, C, Izaurralde, E, Adachi, Y, Wingfield, P, Laemmli, U K

The human immunodeficiency virus type 1 (HIV) Rev protein is thought to be involved in the export of unspliced or singly spliced viral mRNAs from the nucleus to the cytoplasm. This function is...

Hepatitis B virus antigens made in microbial cells immunise against viral infection.

Murray, K, Bruce, S A, Hinnen, A, Wingfield, P, Van Erd, P M, De Reus, A, ...

Chimpanzees have been vaccinated successfully against hepatitis B virus with preparations of the viral antigens made in microbial cells by genetic engineering methods.

Characterization of recombinant-derived granulocyte-colony stimulating factor (G-CSF).

Wingfield, P, Benedict, R, Turcatti, G, Allet, B, Mermod, J J, DeLamarter, J, ...

Human granulocyte colony-stimulating factor (G-CSF), and a mutant having a Ser for Cys substitution at residue 18 were produced in Escherichia coli strain W3110. About 60 mg of pure protein was...

Characterization of human and mouse granulocyte-macrophage-colony-stimulating factors derived from Escherichia coli.

Schrimsher, J L, Rose, K, Simona, M G, Wingfield, P

Human and mouse granulocyte-macrophage-colony-stimulating factors (hGM-CSF and mGM-CSF, respectively), isolated from Escherichia coli cells expressing the corresponding human and mouse genes, have...

C-terminal peptide identification by fast atom bombardment mass spectrometry.

Rose, K, Savoy, L A, Simona, M G, Offord, R E, Wingfield, P

A previously described technique [Rose, Simona, Offord, Prior, Otto & Thatcher (1983) Biochem. J. 215, 273-277] permits the identification of the C-terminal peptide of a protein as the only peptide...

Three-dimensional solution structure of the HIV-1 protease complexed with DMP323, a novel cyclic urea-type inhibitor, determined by nuclear magnetic resonance spectroscopy.

Yamazaki, T., Hinck, A. P., Wang, Y. X., Nicholson, L. K., Torchia, D. A., Wingfield, P., ...

The three-dimensional solution structure of the HIV-1 protease homodimer, MW 22.2 kDa, complexed to a potent, cyclic urea-based inhibitor, DMP323, is reported. This is the first solution structure of...

Monomer-trimer equilibrium of the ectodomain of SIV gp41: insight into the mechanism of peptide inhibition of HIV infection.

Caffrey, M., Kaufman, J., Stahl, S., Wingfield, P., Gronenborn, A. M., Clore, G. M.

The monomer-trimer equilibrium of the ectodomain of SIV gp41 (residues 27-149, e-gp41) has been characterized by analytical ultracentrifugation, circular dichroism (CD), and NMR spectroscopy. Based...