Pamela Stanley

Mutational and functional analysis of Large in a novel CHO glycosylation mutant (2009)

Aguilan, Jennifer T, Sundaram, Subha, Nieves, Edward, Stanley, Pamela

Inactivating mutations of Large reduce the functional glycosylation of α-dystroglycan (α-DG) and lead to muscular dystrophy in mouse and humans. The N-terminal domain of Large is most similar to...

In vivoconsequences of deleting EGF repeats 8–12 including the ligand binding domain of mouse Notch1 (2008)

Ge, Changhui, Liu, Tongyi, Hou, Xinghua, Stanley, Pamela

Abstract Background Notch signaling is highly conserved in the metazoa and is critical for many cell fate decisions. Notch activation occurs following ligand binding to Notch extracellular domain. In...

Complex N-glycans are the major ligands for galectin-1, -3, and -8 on Chinese hamster ovary cells (2006)

Patnaik, Santosh Kumar, Potvin, Barry, Carlsson, Susanne, Sturm, David, Leffler, Hakon, Stanley, Pamela

Galectins are implicated in a large variety of biological functions, many of which depend on their carbohydrate-binding ability. Fifteen members of the family have been identified in vertebrates...

Molecular analysis of three gain-of-function CHO mutants that add the bisecting GlcNAc to N-glycans (2005)

Stanley, Pamela, Sundaram, Subha, Tang, Jian, Shi, Shaolin

LEC10 Chinese hamster ovary (CHO) cells are gain-of-function mutants that express N-acetylglucosaminyltransferase III (GlcNAc-TIII), the glycosyltransferase that adds the bisecting GlcNAc to complex...

Complex N-glycans are the Major Ligands for Galectin-1, Galectin-3 and Galectin-8 on Chinese Hamster Ovary Cells (2005)

Patnaik, Santosh Kumar, Potvin, Barry, Carlsson, Susanne, Sturm, David, Leffler, Hakon, Stanley, Pamela

Galectins are implicated in a large variety of biological functions, many of which depend on their carbohydrate binding ability. Fifteen members of the family have been identified in verteberates...

Identification and characterization of novel alpha 1-3 fucosyltransferases / (2004)

Patnaik, Santosh Kumar., Stanley, Pamela.

Advisor: Pamela Stanley.

Molecular analysis of three gain-of-function CHO mutants that add the bisecting GlcNAc to N-glycans (2004)

Stanley, Pamela, Sundaram, Subha, Tang, Jian, Shi, Shaolin

LEC10 Chinese hamster ovary (CHO) cells are gain-of-function mutants that express N-acetylglucosaminyltransferase III (GlcNAc-TIII), the glycosyltransferase that adds the bisecting GlcNAc to complex...

Suppressors of {alpha}(1,3)fucosylation identified by expression cloning in the LEC11B gain-of-function CHO mutant (2004)

Chen, Wei, Tang, Jian, Stanley, Pamela

Factors that regulate α(1,3)fucosyltransferase activity are important to identify because FUT genes are upregulated during inflammation, cancer progression and tumor metastasis. FUT gene activation...

Molecular analysis of three gain-of-function CHO mutants that add the bisecting GlcNAc to N-glycans (2004)

Stanley, Pamela, Sundaram, Subha, Tang, Jian, Shi, Shaolin

LEC10 Chinese hamster ovary (CHO) cells are gain-of-function mutants that express N-acetylglucosaminyltransferase III (GlcNAc-TIII), the glycosyltransferase that adds the bisecting GlcNAc to complex...

Suppressors of {alpha}(1,3)fucosylation identified by expression cloning in the LEC11B gain-of-function CHO mutant (2004)

Chen, Wei, Tang, Jian, Stanley, Pamela

Factors that regulate α(1,3)fucosyltransferase activity are important to identify because FUT genes are upregulated during inflammation, cancer progression and tumor metastasis. FUT gene activation...

Five Lec1 CHO cell mutants have distinct Mgat1 gene mutations that encode truncated N-acetylglucosaminyltransferase I (2003)

Chen, Wei, Stanley, Pamela

Lec1 CHO cell mutants lack N-acetylglucosaminyltransferase I (GlcNAc-TI) activity and do not synthesize complex or hybrid N-glycans. The origins of six independent lec1 mutations are shown to reside...

Biological roles of the bisecting G1cNAc of N-glycans in hepatocarcinogenesis and liver regeneration / (2002)

Yang, Xiaoping., Stanley, Pamela.

Advisor: Pamela Stanley.

Five Lec1 CHO Cell Mutants Have Distinct Mgat1 Gene Mutations That Encode Truncated N-acetylglucosaminyltransferase I (2002)

Chen, Wei, Stanley, Pamela

Lec1 Chinese hamster ovary (CHO) cell mutants lack N-acetylglucosaminyltransferase I (GlcNAc-TI) activity and do not synthesize complex or hybrid N-glycans. The origins of six independent lec1...

A novel missense mutation in lysosomal sulfamidase is the basis of MPS III A in a spontaneous mouse mutant (2001)

Bhattacharyya, Riddhi, Gliddon, B. L., Beccari, T., Hopwood, J. J., Stanley, Pamela.

A novel missense mutation in lysosomal sulfamidase is the basis of MPS III A in a spontaneous mouse mutant (2001)

Bhattacharyya, Riddhi, Gliddon, B. L., Beccari, T., Hopwood, J. J., Stanley, Pamela.

Molecular basis of loss-of-function and gain-of-function CHO glycosylation mutants / (2001)

Chen, Wei., Stanley, Pamela.

Advisor: Pamela Stanley.

A novel missense mutation in lysosomal sulfamidase is the basis of MPS III A in a spontaneous mouse mutant (2001)

Bhattacharyya, Riddhi, Gliddon, Briony, Beccari, Tommaso, Hopwood, John J., Stanley, Pamela

Sanfilippo syndrome type III A (Mucopolysaccharidosis (MPS) III A) is a rare, autosomal recessive, lysosomal storage disease, characterized by the accumulation of heparan sulfate and the loss of...

A mouse model for mucopolysaccharidosistype III A (Sanfilippo syndrome) (1999)

Bhaumik, Mantu, J.Muller, Vivienne, Rozaklis, Tina, Johnson, Linda, Dobrenis, Kostantin, Bhattacharyya, Riddhi, ...

Mucopolysaccharidosis type III A (MPS III A, Sanfilippo syndrome)is a rare, autosomal recessive, lysosomal storage disease characterizedby accumulation of heparan sulfate secondary to defective...

Organization and regulation of Chinese hamster [alpha](1,3)fucosyltransferase genes / (1998)

Zhang, Aimin., Stanley, Pamela.

Advisor: Pamela Stanley.

Complex N-glycans in Mgat1 null preimplantation embryos arise from maternal Mgat1 RNA (1997)

Ioffe, Ella, Liu, Yun, Stanley, Pamela

Mice with a null mutation in the Mgat1 gene lack N-acetyl-glucosaminyltransferase I (GlcNAc-TI; EC 2.4.1.101), and die at mid-gestation. This result suggested that development of Mgat1−1−...

CHO cells provide access to novel N-glycans and developmentally regulated glycosyltransferases (1996)

Stanley, Pamela, Raju, T.Shantha, Bhaumik, Mantu

Chinese hamster ovary (CHO) cells express only a subset of the glycosyltransferase activities known to exist. They do not express several fucosyltransferases, galactosyltransferases,...

Identification of essential roles for N-linked carbohydrates in mouse development /--by Ella Ioffe. (1995)

Ioffe, Ella., Stanley, Pamela.

Advisor: Pamela Stanley.

Regulation of N-linked glycosylation. Neuronal cell-specific expression of a 5' extended transcript from the gene encoding N-acetylglucosaminyltranserase I (1995)

Yang, Jing, Bhaumik, Mantu, Liu, Yun, Stanley, Pamela

During the reproduction process, several of the major bands in Figure 2 of the paper became difficult to discern. An earlier photograph of the same blot is presented below.

Regulation of N-linked glycosylation. Neuronal cell-specific expression of a 5' extended transcript from the gene encoding N-acetylglucosaminyltransferase I (1994)

Yang, Jing, Bhaumik, Mantu, Liu, Yun, Stanley, Pamela

A key transferase in the generation of mature N-linked carbohydrates is the medial Golgi enzyme N-acetylglucos-aminyltransferase I (EC 2.4.1.101; GlcNAc-TI) which is encoded by the Mgat-1 gene....

Mutants in dolichol synthesis: conversion of polyprenol to dolichol appears to be a rate-limiting step in dolichol synthesis (1993)

Rosenwald, Anne G., Stanley, Pamela, McLachlan, Karen R., Krag, Sharon S.

Chinese hamster ovary (CHO) cells of the Lec9 recessive complementation group display a distinctive profile of resistance to a variety of toxic lectins. In addition, they accumulate...

Glycosylation engineering (1992)

Stanley, Pamela

Cloning and expression of the murine gene and chromosomal location of the human gene encoding N-acetylglucosaminyltransferase I (1992)

Kumar, Ravindra, Yang, Jing, Eddy, Roger L., Byers, Mary G., Shows, Thomas B., Stanley, Pamela

A mouse cDNA clone previously isolated from an F9 teratocarcinoma cell library and shown to confer N-acetylglucosaminyltransferase I (GlcNAc-TI) activity on Lec1 Chinese hamster ovary (CHO) cell...

A subclass of cell surface carbohydrates revealed by a CHO mutant with two glycosylation mutations (1991)

Stanley, Pamela, Sundaram, Subha, Sallustio, Sandra

A novel lectin-resistance phenotype was displayed by a LEC10 Chinese hamster ovary (CHO) cell mutant that was selected for resistance to the erythroagglutinin, E-PHA. Biochemical and genetic analyses...

Mammalian cytidine 5′-monophosphate N-acetylneuraminic acid synthetase: A nuclear protein with evolutionarily conserved structural motifs

Münster, Anja-K., Eckhardt, Matthias, Potvin, Barry, Mühlenhoff, Martina, Stanley, Pamela, Gerardy-Schahn, Rita

Sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. The pattern of cell surface sialylation is species- and...

Fringe modulation of Jagged1-induced Notch signaling requires the action of β4galactosyltransferase-1

Chen, Jihua, Moloney, Daniel J., Stanley, Pamela

Fringe modulates Notch signaling resulting in the establishment of compartmental boundaries in developing organisms. Fringe is a β3N-acetylglucosaminyltransferase (β3GlcNAcT) that transfers GlcNAc...

Protein O-fucosyltransferase 1 is an essential component of Notch signaling pathways

Shi, Shaolin, Stanley, Pamela

Notch receptor signaling regulates cell growth and differentiation, and core components of Notch signaling pathways are conserved from Drosophila to humans. Fringe glycosyltransferases are crucial...

Inactivation of the Mgat1 Gene in Oocytes Impairs Oogenesis, but Embryos Lacking Complex and Hybrid N-Glycans Develop and Implant

Shi, Shaolin, Williams, Suzannah A., Seppo, Antti, Kurniawan, Henry, Chen, Wei, Ye, Zhengyi, ...

Complex and hybrid N-glycans contain sugar residues that have been implicated in fertilization, compaction of the embryo, and implantation. Inactivation of the Mgat1 gene responsible for their...

Inactivation of the Mgat1 Gene in Oocytes Impairs Oogenesis, but Embryos Lacking Complex and Hybrid N-Glycans Develop and Implant

Shi, Shaolin, Williams, Suzannah A., Seppo, Antti, Kurniawan, Henry, Chen, Wei, Ye, Zhengyi, ...

Canonical Notch Signaling Is Dispensable for Early Cell Fate Specifications in Mammals

Shi, Shaolin, Stahl, Mark, Lu, Linchao, Stanley, Pamela

The canonical Notch signaling pathway mediated by Delta- and Jagged-like Notch ligands determines a variety of cell fates in metazoa. In Caenorhabditis elegans and sea urchins, canonical Notch...

Mammalian cytidine 5′-monophosphate N-acetylneuraminic acid synthetase: A nuclear protein with evolutionarily conserved structural motifs

Münster, Anja-K., Eckhardt, Matthias, Potvin, Barry, Mühlenhoff, Martina, Stanley, Pamela, Gerardy-Schahn, Rita

Sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. The pattern of cell surface sialylation is species- and...

Fringe modulation of Jagged1-induced Notch signaling requires the action of β4galactosyltransferase-1

Chen, Jihua, Moloney, Daniel J., Stanley, Pamela

Fringe modulates Notch signaling resulting in the establishment of compartmental boundaries in developing organisms. Fringe is a β3N-acetylglucosaminyltransferase (β3GlcNAcT) that transfers GlcNAc...

Protein O-fucosyltransferase 1 is an essential component of Notch signaling pathways

Shi, Shaolin, Stanley, Pamela

Notch receptor signaling regulates cell growth and differentiation, and core components of Notch signaling pathways are conserved from Drosophila to humans. Fringe glycosyltransferases are crucial...

Inactivation of the Mgat1 Gene in Oocytes Impairs Oogenesis, but Embryos Lacking Complex and Hybrid N-Glycans Develop and Implant

Shi, Shaolin, Williams, Suzannah A., Seppo, Antti, Kurniawan, Henry, Chen, Wei, Ye, Zhengyi, ...

Complex and hybrid N-glycans contain sugar residues that have been implicated in fertilization, compaction of the embryo, and implantation. Inactivation of the Mgat1 gene responsible for their...

Inactivation of the Mgat1 Gene in Oocytes Impairs Oogenesis, but Embryos Lacking Complex and Hybrid N-Glycans Develop and Implant

Shi, Shaolin, Williams, Suzannah A., Seppo, Antti, Kurniawan, Henry, Chen, Wei, Ye, Zhengyi, ...

Canonical Notch Signaling Is Dispensable for Early Cell Fate Specifications in Mammals

Shi, Shaolin, Stahl, Mark, Lu, Linchao, Stanley, Pamela

The canonical Notch signaling pathway mediated by Delta- and Jagged-like Notch ligands determines a variety of cell fates in metazoa. In Caenorhabditis elegans and sea urchins, canonical Notch...

In vivo consequences of deleting EGF repeats 8–12 including the ligand binding domain of mouse Notch1

Ge, Changhui, Liu, Tongyi, Hou, Xinghua, Stanley, Pamela

The O-fucose glycan in the ligand-binding domain of Notch1 regulates embryogenesis and T cell development

Ge, Changhui, Stanley, Pamela

Mechanisms by which the extracellular domain of Notch1 controls Notch1 signaling are not well defined. Here, we show that the O-fucose glycan in the Notch1 ligand-binding domain regulates the...

Roles of Pofut1 and O-Fucose in Mammalian Notch Signaling*S⃞

Stahl, Mark, Uemura, Kazuhide, Ge, Changhui, Shi, Shaolin, Tashima, Yuko, Stanley, Pamela

Mammalian Notch receptors contain 29–36 epidermal growth factor (EGF)-like repeats that may be modified by protein O-fucosyltransferase 1 (Pofut1), an essential component of the canonical Notch...