Exploiting Thiol Modifications (2004)
Patricia J. Kiley, Gisela Storz
Molecular oxygen may be necessary for life but with its beneficial properties comes formation of potentially toxic reactive oxygen species. One of the ways in which bacteria protect themselves is...
Yisheng Kang, K. Derek Weber, Yu Qiu, Patricia J. Kiley, Frederick R. Blattner
The major regulator controlling the physiological switch between aerobic and anaerobic growth conditions in Escherichia coli is the DNA binding protein FNR. To identify genes controlled by FNR, we...
Darwin, Andrew J., Ziegelhoffer, Eva C., Kiley, Patricia J., Stewart, Valley
The expression of several Escherichia coli operons is activated by the Fnr protein during anaerobic growth and is further controlled in response to nitrate and nitrite by the homologous response...
Schwartz, Christopher J., Djaman, Ouliana, Imlay, James A., Kiley, Patricia J.
The cysteine desulfurase, IscS, provides sulfur for Fe-S cluster synthesis in vitro, but a role for IscS in in vivo Fe-S cluster formation has yet to be established. To study the in vivo function of...
Khoroshilova, Natalia, Popescu, Codrina, Münck, Eckard, Beinert, Helmut, Kiley, Patricia J.
The transcription factor FNR (fumarate nitrate reduction) requires the presence of an iron-sulfur (Fe-S) cluster for its function as a global transcription regulator in Escherichia coli when oxygen...
Popescu, Codrina V., Bates, Donna M., Beinert, Helmut, Münck, Eckard, Kiley, Patricia J.
The global regulator FNR (for fumarate nitrate reduction) controls the transcription of >100 genes whose products facilitate adaptation of Escherichia coli to growth under O2-limiting conditions....
Schwartz, Christopher J., Giel, Jennifer L., Patschkowski, Thomas, Luther, Christopher, Ruzicka, Frank J., Beinert, Helmut, ...
IscR (iron-sulfur cluster regulator) is encoded by an ORF located immediately upstream of genes coding for the Escherichia coli Fe-S cluster assembly proteins, IscS, IscU, and IscA. IscR shares amino...
Darwin, Andrew J., Ziegelhoffer, Eva C., Kiley, Patricia J., Stewart, Valley
The expression of several Escherichia coli operons is activated by the Fnr protein during anaerobic growth and is further controlled in response to nitrate and nitrite by the homologous response...
Exploiting Thiol Modifications
Kiley, Patricia J, Storz, Gisela
Molecular oxygen may be necessary for life but with its beneficial properties comes formation of potentially toxic reactive oxygen species. One of the ways in which bacteria protect themselves is...
Sutton, Victoria R., Mettert, Erin L., Beinert, Helmut, Kiley, Patricia J.
The ability of FNR to sense and respond to cellular O2 levels depends on its [4Fe-4S]2+ cluster. In the presence of O2, the [4Fe-4S]2+ cluster is converted to a [2Fe-2S]2+ cluster, which inactivates...
Kang, Yisheng, Weber, K. Derek, Qiu, Yu, Kiley, Patricia J., Blattner, Frederick R.
The major regulator controlling the physiological switch between aerobic and anaerobic growth conditions in Escherichia coli is the DNA binding protein FNR. To identify genes controlled by FNR, we...
Weber, K. Derek, Vincent, Owen D., Kiley, Patricia J.
The global anaerobic regulator FNR is a DNA binding protein that activates transcription of genes required for anaerobic metabolism in Escherichia coli through interactions with RNA polymerase...
Schwartz, Christopher J., Djaman, Ouliana, Imlay, James A., Kiley, Patricia J.
The cysteine desulfurase, IscS, provides sulfur for Fe-S cluster synthesis in vitro, but a role for IscS in in vivo Fe-S cluster formation has yet to be established. To study the in vivo function of...
Khoroshilova, Natalia, Popescu, Codrina, Münck, Eckard, Beinert, Helmut, Kiley, Patricia J.
The transcription factor FNR (fumarate nitrate reduction) requires the presence of an iron-sulfur (Fe-S) cluster for its function as a global transcription regulator in Escherichia coli when oxygen...
Popescu, Codrina V., Bates, Donna M., Beinert, Helmut, Münck, Eckard, Kiley, Patricia J.
The global regulator FNR (for fumarate nitrate reduction) controls the transcription of >100 genes whose products facilitate adaptation of Escherichia coli to growth under O2-limiting conditions....
Schwartz, Christopher J., Giel, Jennifer L., Patschkowski, Thomas, Luther, Christopher, Ruzicka, Frank J., Beinert, Helmut, ...
IscR (iron-sulfur cluster regulator) is encoded by an ORF located immediately upstream of genes coding for the Escherichia coli Fe-S cluster assembly proteins, IscS, IscU, and IscA. IscR shares amino...
Darwin, Andrew J., Ziegelhoffer, Eva C., Kiley, Patricia J., Stewart, Valley
The expression of several Escherichia coli operons is activated by the Fnr protein during anaerobic growth and is further controlled in response to nitrate and nitrite by the homologous response...
Exploiting Thiol Modifications
Kiley, Patricia J, Storz, Gisela
Molecular oxygen may be necessary for life but with its beneficial properties comes formation of potentially toxic reactive oxygen species. One of the ways in which bacteria protect themselves is...
Sutton, Victoria R., Mettert, Erin L., Beinert, Helmut, Kiley, Patricia J.
The ability of FNR to sense and respond to cellular O2 levels depends on its [4Fe-4S]2+ cluster. In the presence of O2, the [4Fe-4S]2+ cluster is converted to a [2Fe-2S]2+ cluster, which inactivates...
Kang, Yisheng, Weber, K. Derek, Qiu, Yu, Kiley, Patricia J., Blattner, Frederick R.
The major regulator controlling the physiological switch between aerobic and anaerobic growth conditions in Escherichia coli is the DNA binding protein FNR. To identify genes controlled by FNR, we...
Weber, K. Derek, Vincent, Owen D., Kiley, Patricia J.
The global anaerobic regulator FNR is a DNA binding protein that activates transcription of genes required for anaerobic metabolism in Escherichia coli through interactions with RNA polymerase...
Contributions of [4Fe-4S]-FNR and Integration Host Factor to fnr Transcriptional Regulation▿
Mettert, Erin L., Kiley, Patricia J.
Maintaining appropriate levels of the global regulator FNR is critical to its function as an O2 sensor. In this study, we examined the mechanisms that control transcription of fnr to increase our...
Yoon, Sang Sun, Karabulut, Ahmet C, Lipscomb, John D, Hennigan, Robert F, Lymar, Sergei V, Groce, Stephanie L, ...
Protection from NO gas, a toxic byproduct of anaerobic respiration in Pseudomonas aeruginosa, is mediated by nitric oxide (NO) reductase (NOR), the norCB gene product. Nevertheless, a norCB mutant...
Yan, Aixin, Kiley, Patricia J.
The role of the N-terminal region of the transcription factor FNR, which immediately precedes the first ligand (Cys20) of the [4Fe-4S] cluster, was investigated. We found that truncation mutants that...