Role of L2 cysteines in papillomavirus infection and neutralization (2009)
Gambhira, Ratish, Jagu, Subhashini, Karanam, Balasubramanyam, Day, Patricia M, Roden, Richard
Abstract Vaccination of mice with minor capsid protein L2 or passive transfer with the L2-specific neutralizing monoclonal antibody RG-1 protects against human papillomavirus type 16 (HPV16)...
Day, Patricia M., Yewdell, Jonathan W., Porgador, Angel, Germain, Ronald N., Bennink, Jack R.
After brief incubation of cells with fluorescein-conjugated peptides that bind major histocompatibility complex (MHC) class I molecules, peptides were detected within the endoplasmic reticulum (ER)...
Day, Patricia M., Roden, Richard B. S., Lowy, Douglas R., Schiller, John T.
We have used immunofluorescent staining and confocal microscopy to examine the subcellular localization of structural and nonstructural bovine papillomavirus (BPV) proteins in cultured cells that...
L1 Interaction Domains of Papillomavirus L2 Necessary for Viral Genome Encapsidation
Okun, Martin M., Day, Patricia M., Greenstone, Heather L., Booy, Frank P., Lowy, Douglas R., Schiller, John T., ...
BPHE-1 cells, which harbor 50 to 200 viral episomes, encapsidate viral genome and generate infectious bovine papillomavirus type 1 (BPV1) upon coexpression of capsid proteins L1 and L2 of BPV1, but...
Positively Charged Termini of the L2 Minor Capsid Protein Are Necessary for Papillomavirus Infection
Roden, Richard B. S., Day, Patricia M., Bronzo, Brian K., Yutzy, William H., Yang, Yanqin, Lowy, Douglas R., ...
Coexpression of bovine papillomavirus L1 with L2 mutants lacking either eight N-terminal or nine C-terminal amino acids that encode positively charged domains resulted in wild-type levels of viral...
Cell Surface-Binding Motifs of L2 That Facilitate Papillomavirus Infection
Yang, Rongcun, Day, Patricia M., Yutzy IV, William H., Lin, Ken-Yu, Hung, Chien-Fu, Roden, Richard B. S.
Human papillomavirus type 16 (HPV16) is the primary etiologic agent of cervical carcinoma, whereas bovine papillomavirus type 1 (BPV1) causes benign fibropapillomas. However, the capsid proteins, L1...
Day, Patricia M., Baker, Carl C., Lowy, Douglas R., Schiller, John T.
Previous studies have suggested that most papillomaviruses enter the host cell via clathrin-dependent receptor-mediated endocytosis but have not addressed later steps in viral entry. To examine these...
Kämper, Nadine, Day, Patricia M., Nowak, Thorsten, Selinka, Hans-Christoph, Florin, Luise, Bolscher, Jan, ...
Papillomaviruses are internalized via clathrin-dependent endocytosis. However, the mechanism by which viral genomes pass endosomal membranes has not been elucidated. In this report we show that the...
Human α-defensins block papillomavirus infection
Buck, Christopher B., Day, Patricia M., Thompson, Cynthia D., Lubkowski, Jacek, Lu, Wuyuan, Lowy, Douglas R., ...
Sexually transmitted human papillomaviruses (HPVs) are the primary cause of cervical cancer. Recent advances in techniques for production of papillomaviral vectors [known as pseudoviruses (PsVs)]...
Richards, Rebecca M., Lowy, Douglas R., Schiller, John T., Day, Patricia M.
Papillomaviruses (PV) comprise a large family of nonenveloped DNA viruses that include the oncogenic PV types that are the causative agents of human cervical cancer. As is true of many animal DNA...
Day, Patricia M., Yewdell, Jonathan W., Porgador, Angel, Germain, Ronald N., Bennink, Jack R.
After brief incubation of cells with fluorescein-conjugated peptides that bind major histocompatibility complex (MHC) class I molecules, peptides were detected within the endoplasmic reticulum (ER)...
Day, Patricia M., Roden, Richard B. S., Lowy, Douglas R., Schiller, John T.
We have used immunofluorescent staining and confocal microscopy to examine the subcellular localization of structural and nonstructural bovine papillomavirus (BPV) proteins in cultured cells that...
L1 Interaction Domains of Papillomavirus L2 Necessary for Viral Genome Encapsidation
Okun, Martin M., Day, Patricia M., Greenstone, Heather L., Booy, Frank P., Lowy, Douglas R., Schiller, John T., ...
BPHE-1 cells, which harbor 50 to 200 viral episomes, encapsidate viral genome and generate infectious bovine papillomavirus type 1 (BPV1) upon coexpression of capsid proteins L1 and L2 of BPV1, but...
Positively Charged Termini of the L2 Minor Capsid Protein Are Necessary for Papillomavirus Infection
Roden, Richard B. S., Day, Patricia M., Bronzo, Brian K., Yutzy, William H., Yang, Yanqin, Lowy, Douglas R., ...
Coexpression of bovine papillomavirus L1 with L2 mutants lacking either eight N-terminal or nine C-terminal amino acids that encode positively charged domains resulted in wild-type levels of viral...
Cell Surface-Binding Motifs of L2 That Facilitate Papillomavirus Infection
Yang, Rongcun, Day, Patricia M., Yutzy IV, William H., Lin, Ken-Yu, Hung, Chien-Fu, Roden, Richard B. S.
Human papillomavirus type 16 (HPV16) is the primary etiologic agent of cervical carcinoma, whereas bovine papillomavirus type 1 (BPV1) causes benign fibropapillomas. However, the capsid proteins, L1...
Day, Patricia M., Baker, Carl C., Lowy, Douglas R., Schiller, John T.
Previous studies have suggested that most papillomaviruses enter the host cell via clathrin-dependent receptor-mediated endocytosis but have not addressed later steps in viral entry. To examine these...
Kämper, Nadine, Day, Patricia M., Nowak, Thorsten, Selinka, Hans-Christoph, Florin, Luise, Bolscher, Jan, ...
Papillomaviruses are internalized via clathrin-dependent endocytosis. However, the mechanism by which viral genomes pass endosomal membranes has not been elucidated. In this report we show that the...
Human α-defensins block papillomavirus infection
Buck, Christopher B., Day, Patricia M., Thompson, Cynthia D., Lubkowski, Jacek, Lu, Wuyuan, Lowy, Douglas R., ...
Sexually transmitted human papillomaviruses (HPVs) are the primary cause of cervical cancer. Recent advances in techniques for production of papillomaviral vectors [known as pseudoviruses (PsVs)]...
Richards, Rebecca M., Lowy, Douglas R., Schiller, John T., Day, Patricia M.
Papillomaviruses (PV) comprise a large family of nonenveloped DNA viruses that include the oncogenic PV types that are the causative agents of human cervical cancer. As is true of many animal DNA...
Day, Patricia M., Thompson, Cynthia D., Buck, Christopher B., Pang, Yuk-Ying S., Lowy, Douglas R., Schiller, John T.
The mechanisms of human papillomavirus (HPV) neutralization by antibodies are incompletely understood. We have used HPV16 pseudovirus infection of HaCaT cells to analyze how several neutralizing...
Intracellular Localization of Proteasomal Degradation of a Viral Antigen
Antón, Luis C., Schubert, Ulrich, Bacík, Igor, Princiotta, Michael F., Wearsch, Pamela A., Gibbs, James, ...
To better understand proteasomal degradation of nuclear proteins and viral antigens we studied mutated forms of influenza virus nucleoprotein (NP) that misfold and are rapidly degraded by...
Day, Patricia M., Gambhira, Ratish, Roden, Richard B. S., Lowy, Douglas R., Schiller, John T.
Pseudovirions of human papillomavirus type 16 (HPV16), the principal etiologic agent in 50% of cervical cancers, were used as a model system to investigate the cell surface interactions involved in...
Human Papillomavirus Type 16 Entry: Retrograde Cell Surface Transport along Actin-Rich Protrusions
Schelhaas, Mario, Ewers, Helge, Rajamäki, Minna-Liisa, Day, Patricia M., Schiller, John T., Helenius, Ari
The lateral mobility of individual, incoming human papillomavirus type 16 pseudoviruses (PsV) bound to live HeLa cells was studied by single particle tracking using fluorescence video microscopy. The...
Heparan Sulfate-Independent Cell Binding and Infection with Furin-Precleaved Papillomavirus Capsids▿
Day, Patricia M., Lowy, Douglas R., Schiller, John T.
Papillomavirus infection normally involves virion binding to cell surface heparan sulfate proteoglycans (HSPGs). However, we found that human papillomavirus type 16 pseudovirions efficiently bound...
Johnson, Katherine M., Kines, Rhonda C., Roberts, Jeffrey N., Lowy, Douglas R., Schiller, John T., Day, Patricia M.
The host factors required for in vivo infection have not been investigated for any papillomavirus. Using a recently developed murine cervicovaginal challenge model, we evaluated the importance of...
Role of L2 cysteines in papillomavirus infection and neutralization
Gambhira, Ratish, Jagu, Subhashini, Karanam, Balasubramanyam, Day, Patricia M, Roden, Richard
Vaccination of mice with minor capsid protein L2 or passive transfer with the L2-specific neutralizing monoclonal antibody RG-1 protects against human papillomavirus type 16 (HPV16) challenge. Here...