Patrick Chames

Optimizing the exogenous antigen loading of monocyte-derived dendritic cells (2005)

Dieckmann, Detlef, Schultz, Erwin S., Ring, Bernhard, Chames, Patrick, Held, Gerhard, Hoogenboom, Hennie R., ...

Dendritic cell (DC) vaccination, i.e. the adoptive transfer of antigen-loaded DC, is still at an early stage and requires standardization. In this study, we investigated the exogenous loading of...

In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination (2005)

Chames, Patrick, Epinat, Jean-Charles, Guillier, Sophie, Patin, Amélie, Lacroix, Emmanuel, Pâques, Frédéric

Homing endonucleases, endonucleases capable of recognizing long DNA sequences, have been shown to be a tool of choice for precise and efficient genome engineering. Consequently, the possibility to...

Optimizing the exogenous antigen loading of monocyte-derived dendritic cells (2005)

Dieckmann, Detlef, Schultz, Erwin. S., Ring, Bernhard, Chames, Patrick, Held, Gerhard, Hoogenboom, Hennie. R., ...

Dendritic cell (DC) vaccination, i.e. the adoptive transfer of antigen-loaded DC, is still at an early stage and requires standardization. In this study, we investigated the exogenous loading of...

A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells (2003)

Epinat, Jean-Charles, Arnould, Sylvain, Chames, Patrick, Rochaix, Pascal, Desfontaines, Dominique, Puzin, Clémence, ...

Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site‐ specific DNA double‐strand breaks...

Direct selection of a human antibody fragment directed against the tumor T-cell epitope HLA-A1–MAGE-A1 from a nonimmunized phage-Fab library

Chames, Patrick, Hufton, Simon E., Coulie, Pierre G., Uchanska-Ziegler, Barbara, Hoogenboom, Hennie R.

Antitumor antibodies with the same specificity as cytotoxic T lymphocytes that recognize antigenic peptides encoded by tumor-associated genes and presented by MHC class I molecules would be valuable...

Intracellular Immunization of Prokaryotic Cells against a Bacteriotoxin

Chames, Patrick, Fieschi, Jacques, Baty, Daniel, Duché, Denis

Intracellularly expressed antibodies have been designed to bind and inactivate target molecules inside eukaryotic cells. Here we report that an antibody fragment can be used to probe the periplasmic...

Direct visualization of distinct T cell epitopes derived from a melanoma tumor-associated antigen by using human recombinant antibodies with MHC- restricted T cell receptor-like specificity

Denkberg, Galit, Cohen, Cyril J., Lev, Avital, Chames, Patrick, Hoogenboom, Hennie R., Reiter, Yoram

Specificity in the cellular immune system is controlled and regulated by the T cell antigen receptor (TCR), which specifically recognizes peptide/major histocompatibility complex (MHC) molecules. In...

A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells

Epinat, Jean-Charles, Arnould, Sylvain, Chames, Patrick, Rochaix, Pascal, Desfontaines, Dominique, Puzin, Clémence, ...

Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site- specific DNA double-strand breaks (DSBs) have...

In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination

Chames, Patrick, Epinat, Jean-Charles, Guillier, Sophie, Patin, Amélie, Lacroix, Emmanuel, Pâques, Frédéric

Homing endonucleases, endonucleases capable of recognizing long DNA sequences, have been shown to be a tool of choice for precise and efficient genome engineering. Consequently, the possibility to...

Direct selection of a human antibody fragment directed against the tumor T-cell epitope HLA-A1–MAGE-A1 from a nonimmunized phage-Fab library

Chames, Patrick, Hufton, Simon E., Coulie, Pierre G., Uchanska-Ziegler, Barbara, Hoogenboom, Hennie R.

Antitumor antibodies with the same specificity as cytotoxic T lymphocytes that recognize antigenic peptides encoded by tumor-associated genes and presented by MHC class I molecules would be valuable...

Intracellular Immunization of Prokaryotic Cells against a Bacteriotoxin

Chames, Patrick, Fieschi, Jacques, Baty, Daniel, Duché, Denis

Intracellularly expressed antibodies have been designed to bind and inactivate target molecules inside eukaryotic cells. Here we report that an antibody fragment can be used to probe the periplasmic...

Direct visualization of distinct T cell epitopes derived from a melanoma tumor-associated antigen by using human recombinant antibodies with MHC- restricted T cell receptor-like specificity

Denkberg, Galit, Cohen, Cyril J., Lev, Avital, Chames, Patrick, Hoogenboom, Hennie R., Reiter, Yoram

Specificity in the cellular immune system is controlled and regulated by the T cell antigen receptor (TCR), which specifically recognizes peptide/major histocompatibility complex (MHC) molecules. In...

A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells

Epinat, Jean-Charles, Arnould, Sylvain, Chames, Patrick, Rochaix, Pascal, Desfontaines, Dominique, Puzin, Clémence, ...

Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site- specific DNA double-strand breaks (DSBs) have...

In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination

Chames, Patrick, Epinat, Jean-Charles, Guillier, Sophie, Patin, Amélie, Lacroix, Emmanuel, Pâques, Frédéric

Homing endonucleases, endonucleases capable of recognizing long DNA sequences, have been shown to be a tool of choice for precise and efficient genome engineering. Consequently, the possibility to...

A combinatorial approach to create artificial homing endonucleases cleaving chosen sequences

Smith, Julianne, Grizot, Sylvestre, Arnould, Sylvain, Duclert, Aymeric, Epinat, Jean-Charles, Chames, Patrick, ...

Meganucleases, or homing endonucleases (HEs) are sequence-specific endonucleases with large (>14 bp) cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells...