Paul Blum

Review of Archaea: Molecular and Cellular Biology (2008)

Blum, Paul

Archaea along with bacteria comprise the two groups of prokaryotes. The discovery of the Archaea, now over 30 years ago, has blossomed into fascinating revelations about the origin of life and the...

Flagellar Motility and Structure in the Hyperthermoacidophilic Archaeon Sulfolobus solfataricus (2007)

Szabó, Zalán, Sani, Musa, Groeneveld, Maarten, Zolghadr, Benham, Schelert, James, Albers, Sonja-Verena, ...

Flagellation in archaea is widespread and is involved in swimming motility. Here, we demonstrate that the structural flagellin gene from the crenarchaeaon Sulfolobus solfataricus is highly expressed...

La piel / por Paul Blum (1973)

Blum, Paul

Traducción de: "La Peau"

Coordinate Transcriptional Control in the Hyperthermophilic Archaeon Sulfolobus solfataricus

Haseltine, Cynthia, Montalvo-Rodriguez, Rafael, Bini, Elisabetta, Carl, Audrey, Blum, Paul

The existence of a global gene regulatory system in the hyperthermophilic archaeon Sulfolobus solfataricus is described. The system is responsive to carbon source quality and acts at the level of...

Secreted Euryarchaeal Microhalocins Kill Hyperthermophilic Crenarchaea

Haseltine, Cynthia, Hill, Tiffany, Montalvo-Rodriguez, Rafael, Kemper, Samantha K., Shand, Richard F., Blum, Paul

Few antibiotics targeting members of the archaeal domain are currently available for genetic studies. Since bacterial antibiotics are frequently directed against competing and related organisms,...

Bacterial Growth State Distinguished by Single-Cell Protein Profiling: Does Chlorination Kill Coliforms in Municipal Effluent?

Rockabrand, David, Austin, Teresa, Kaiser, Robyn, Blum, Paul

Municipal effluent is the largest reservoir of human enteric bacteria. Its public health significance, however, depends upon the physiological status of the wastewater bacterial community. A novel...

Roles of DnaK and RpoS in Starvation-Induced Thermotolerance of Escherichia coli

Rockabrand, David, Livers, Kevin, Austin, Tess, Kaiser, Robyn, Jensen, Debra, Burgess, Richard, ...

DnaK is essential for starvation-induced resistance to heat, oxidation, and reductive division in Escherichia coli. Studies reported here indicate that DnaK is also required for starvation-induced...

Molecular Characterization of the α-Glucosidase Gene (malA) from the Hyperthermophilic Archaeon Sulfolobus solfataricus

Rolfsmeier, Michael, Haseltine, Cynthia, Bini, Elisabetta, Clark, Amy, Blum, Paul

Acidic hot springs are colonized by a diversity of hyperthermophilic organisms requiring extremes of temperature and pH for growth. To clarify how carbohydrates are consumed in such locations, the...

Targeted Disruption of the α-Amylase Gene in the Hyperthermophilic Archaeon Sulfolobus solfataricus

Worthington, Penny, Hoang, Viet, Perez-Pomares, Francisco, Blum, Paul

Sulfolobus solfataricus secretes an acid-resistant α-amylase (amyA) during growth on starch as the sole carbon and energy source. Synthesis of this activity is subject to catabolite repression. To...

Large-Scale Cultivation of Acidophilic Hyperthermophiles for Recovery of Secreted Proteins

Worthington, Penny, Blum, Paul, Perez-Pomares, Francisco, Elthon, Tom

An electric water heater was modified for large-scale cultivation of aerobic acidophilic hyperthermophiles to enable recovery of secreted proteins. Critical changes included thermostat replacement,...

Single-Cell Protein Profiling of Wastewater Enterobacterial Communities Predicts Disinfection Efficiency

Ponniah, Gomathinayagam, Chen, Han, Michielutti, Ronda, Salonen, Nancy, Blum, Paul

The efficiency of enterobacterial disinfection is dependent largely on enterobacterial community physiology. However, the relationship between enterobacterial community physiology and wastewater...

Occurrence and Characterization of Mercury Resistance in the Hyperthermophilic Archaeon Sulfolobus solfataricus by Use of Gene Disruption

Schelert, James, Dixit, Vidula, Hoang, Viet, Simbahan, Jessica, Drozda, Melissa, Blum, Paul

Mercury resistance mediated by mercuric reductase (MerA) is widespread among bacteria and operates under the control of MerR. MerR represents a unique class of transcription factors that exert both...

Mercury Inactivates Transcription and the Generalized Transcription Factor TFB in the Archaeon Sulfolobus solfataricus

Dixit, Vidula, Bini, Elisabetta, Drozda, Melissa, Blum, Paul

Mercury has a long history as an antimicrobial agent effective against eukaryotic and prokaryotic organisms. Despite its prolonged use, the basis for mercury toxicity in prokaryotes is not well...

A freestanding proofreading domain is required for protein synthesis quality control in Archaea

Korencic, Dragana, Ahel, Ivan, Schelert, James, Sacher, Meik, Ruan, Benfang, Stathopoulos, Constantinos, ...

Threonyl-tRNA synthetase (ThrRS) participates in protein synthesis quality control by selectively editing the misacylated species Ser-tRNAThr. In bacteria and eukaryotes the editing function of ThrRS...

Culture-Independent Analysis of Fecal Enterobacteria in Environmental Samples by Single-Cell mRNA Profiling

Chen, Han, Ponniah, Gomathinayagam, Salonen, Nancy, Blum, Paul

A culture-independent method called mRNA profiling has been developed for the analysis of fecal enterobacteria and their physiological status in environmental samples. This taxon-specific approach...

Community Analysis of a Mercury Hot Spring Supports Occurrence of Domain-Specific Forms of Mercuric Reductase

Simbahan, Jessica, Kurth, Elizabeth, Schelert, James, Dillman, Amanda, Moriyama, Etsuko, Jovanovich, Stevan, ...

Mercury is a redox-active heavy metal that reacts with active thiols and depletes cellular antioxidants. Active resistance to the mercuric ion is a widely distributed trait among bacteria and results...

Stability of mRNA in the hyperthermophilic archaeon Sulfolobus solfataricus.

Bini, Elisabetta, Dikshit, Vidula, Dirksen, Kristi, Drozda, Melissa, Blum, Paul

Archaea-like bacteria are prokaryotes but, in contrast, use eukaryotic-like systems for key aspects of DNA, RNA, and protein metabolism. mRNA is typically unstable in bacteria and stable in...

The role of cis-acting sequences governing catabolite repression control of lacS expression in the archaeon Sulfolobus solfataricus.

Hoang, Viet, Bini, Elisabetta, Dixit, Vidula, Drozda, Melissa, Blum, Paul

The archaeon Sulfolobus solfataricus uses a catabolite repression-like system to control production of several glycoside hydrolases. To better understand this regulatory system, studies of the...

Coordinate Transcriptional Control in the Hyperthermophilic Archaeon Sulfolobus solfataricus

Haseltine, Cynthia, Montalvo-Rodriguez, Rafael, Bini, Elisabetta, Carl, Audrey, Blum, Paul

The existence of a global gene regulatory system in the hyperthermophilic archaeon Sulfolobus solfataricus is described. The system is responsive to carbon source quality and acts at the level of...

Secreted Euryarchaeal Microhalocins Kill Hyperthermophilic Crenarchaea

Haseltine, Cynthia, Hill, Tiffany, Montalvo-Rodriguez, Rafael, Kemper, Samantha K., Shand, Richard F., Blum, Paul

Few antibiotics targeting members of the archaeal domain are currently available for genetic studies. Since bacterial antibiotics are frequently directed against competing and related organisms,...

Bacterial Growth State Distinguished by Single-Cell Protein Profiling: Does Chlorination Kill Coliforms in Municipal Effluent?

Rockabrand, David, Austin, Teresa, Kaiser, Robyn, Blum, Paul

Municipal effluent is the largest reservoir of human enteric bacteria. Its public health significance, however, depends upon the physiological status of the wastewater bacterial community. A novel...

Roles of DnaK and RpoS in Starvation-Induced Thermotolerance of Escherichia coli

Rockabrand, David, Livers, Kevin, Austin, Tess, Kaiser, Robyn, Jensen, Debra, Burgess, Richard, ...

DnaK is essential for starvation-induced resistance to heat, oxidation, and reductive division in Escherichia coli. Studies reported here indicate that DnaK is also required for starvation-induced...

Molecular Characterization of the α-Glucosidase Gene (malA) from the Hyperthermophilic Archaeon Sulfolobus solfataricus

Rolfsmeier, Michael, Haseltine, Cynthia, Bini, Elisabetta, Clark, Amy, Blum, Paul

Acidic hot springs are colonized by a diversity of hyperthermophilic organisms requiring extremes of temperature and pH for growth. To clarify how carbohydrates are consumed in such locations, the...

Targeted Disruption of the α-Amylase Gene in the Hyperthermophilic Archaeon Sulfolobus solfataricus

Worthington, Penny, Hoang, Viet, Perez-Pomares, Francisco, Blum, Paul

Sulfolobus solfataricus secretes an acid-resistant α-amylase (amyA) during growth on starch as the sole carbon and energy source. Synthesis of this activity is subject to catabolite repression. To...

Large-Scale Cultivation of Acidophilic Hyperthermophiles for Recovery of Secreted Proteins

Worthington, Penny, Blum, Paul, Perez-Pomares, Francisco, Elthon, Tom

An electric water heater was modified for large-scale cultivation of aerobic acidophilic hyperthermophiles to enable recovery of secreted proteins. Critical changes included thermostat replacement,...

Single-Cell Protein Profiling of Wastewater Enterobacterial Communities Predicts Disinfection Efficiency

Ponniah, Gomathinayagam, Chen, Han, Michielutti, Ronda, Salonen, Nancy, Blum, Paul

The efficiency of enterobacterial disinfection is dependent largely on enterobacterial community physiology. However, the relationship between enterobacterial community physiology and wastewater...

Occurrence and Characterization of Mercury Resistance in the Hyperthermophilic Archaeon Sulfolobus solfataricus by Use of Gene Disruption

Schelert, James, Dixit, Vidula, Hoang, Viet, Simbahan, Jessica, Drozda, Melissa, Blum, Paul

Mercury resistance mediated by mercuric reductase (MerA) is widespread among bacteria and operates under the control of MerR. MerR represents a unique class of transcription factors that exert both...

Mercury Inactivates Transcription and the Generalized Transcription Factor TFB in the Archaeon Sulfolobus solfataricus

Dixit, Vidula, Bini, Elisabetta, Drozda, Melissa, Blum, Paul

Mercury has a long history as an antimicrobial agent effective against eukaryotic and prokaryotic organisms. Despite its prolonged use, the basis for mercury toxicity in prokaryotes is not well...

A freestanding proofreading domain is required for protein synthesis quality control in Archaea

Korencic, Dragana, Ahel, Ivan, Schelert, James, Sacher, Meik, Ruan, Benfang, Stathopoulos, Constantinos, ...

Threonyl-tRNA synthetase (ThrRS) participates in protein synthesis quality control by selectively editing the misacylated species Ser-tRNAThr. In bacteria and eukaryotes the editing function of ThrRS...

Culture-Independent Analysis of Fecal Enterobacteria in Environmental Samples by Single-Cell mRNA Profiling

Chen, Han, Ponniah, Gomathinayagam, Salonen, Nancy, Blum, Paul

A culture-independent method called mRNA profiling has been developed for the analysis of fecal enterobacteria and their physiological status in environmental samples. This taxon-specific approach...

Community Analysis of a Mercury Hot Spring Supports Occurrence of Domain-Specific Forms of Mercuric Reductase

Simbahan, Jessica, Kurth, Elizabeth, Schelert, James, Dillman, Amanda, Moriyama, Etsuko, Jovanovich, Stevan, ...

Mercury is a redox-active heavy metal that reacts with active thiols and depletes cellular antioxidants. Active resistance to the mercuric ion is a widely distributed trait among bacteria and results...

Stability of mRNA in the hyperthermophilic archaeon Sulfolobus solfataricus.

Bini, Elisabetta, Dikshit, Vidula, Dirksen, Kristi, Drozda, Melissa, Blum, Paul

Archaea-like bacteria are prokaryotes but, in contrast, use eukaryotic-like systems for key aspects of DNA, RNA, and protein metabolism. mRNA is typically unstable in bacteria and stable in...

The role of cis-acting sequences governing catabolite repression control of lacS expression in the archaeon Sulfolobus solfataricus.

Hoang, Viet, Bini, Elisabetta, Dixit, Vidula, Drozda, Melissa, Blum, Paul

The archaeon Sulfolobus solfataricus uses a catabolite repression-like system to control production of several glycoside hydrolases. To better understand this regulatory system, studies of the...

Regulation of Mercury Resistance in the Crenarchaeote Sulfolobus solfataricus†

Schelert, James, Drozda, Melissa, Dixit, Vidula, Dillman, Amanda, Blum, Paul

Mercuric ion, Hg(II), inactivates generalized transcription in the crenarchaeote Sulfolobus solfataricus. Metal challenge simultaneously derepresses transcription of mercuric reductase (merA) by...

Flagellar Motility and Structure in the Hyperthermoacidophilic Archaeon Sulfolobus solfataricus▿

Szabó, Zalán, Sani, Musa, Groeneveld, Maarten, Zolghadr, Benham, Schelert, James, Albers, Sonja-Verena, ...

Flagellation in archaea is widespread and is involved in swimming motility. Here, we demonstrate that the structural flagellin gene from the crenarchaeaon Sulfolobus solfataricus is highly expressed...

Something Old, Something New, Something Borrowed; How the Thermoacidophilic Archaeon Sulfolobus solfataricus Responds to Oxidative Stress

Maaty, Walid S., Wiedenheft, Blake, Tarlykov, Pavel, Schaff, Nathan, Heinemann, Joshua, Robison-Cox, Jim, ...

To avoid molecular damage of biomolecules due to oxidation, all cells have evolved constitutive and responsive systems to mitigate and repair chemical modifications. Archaea have adapted to some of...