Paul D. Lampe

Use of cancer-specific yeast-secreted in vivobiotinylated recombinant antibodies for serum biomarker discovery (2008)

Scholler, Nathalie, Gross, Jennifer A, Garvik, Barbara, Wells, Lance, Liu, Yan, Loch, Christian M, ...

Abstract Background Strategies to discover circulating protein markers of ovarian cancer are urgently needed. We developed a novel technology that permits us to isolate recombinant antibodies...

A Cautionary Note on the Evaluation of Biomarkers of Subtypes of a Single Disease (2008)

Adewale, Adeniyi J., Liu, Qi, Dinu, Irina, Lampe, Paul D., Mitchell, Bree L., Yasui, Yutaka

Heterogeneity in the molecular characteristics of a disease presents a challenge to investigators attempting to identify biomarkers of the disease. Preceding the biomarker discovery effort with...

Impact of Freeze-thaw Cycles and Storage Time on Plasma (2005)

Breeana L Mitchell, Yutaka Yasui, Christopher I Li, Annette L. Fitzpatrick, Paul D Lampe

Abstract: Mass spectrometry approaches to biomarker discovery in human fluids have received a great deal of attention in recent years. While mass spectrometry instrumentation and analysis approaches...

Impact of Freeze-thaw Cycles and Storage Time on Plasma Samples Used in Mass Spectrometry Based Biomarker Discovery Projects (2005)

Breeana L Mitchell, Yutaka Yasui, Christopher I Li, Annette L. Fitzpatrick, Paul D Lampe

Abstract: Mass spectrometry approaches to biomarker discovery in human fluids have received a great deal of attention in recent years. While mass spectrometry instrumentation and analysis approaches...

Mice deficient for the gap junction protein Connexin32 exhibit increased radiation-induced tumorigenesis associated with elevated mitogen-activated protein kinase (p44/Erk1, p42/Erk2) activation (2004)

King, Timothy J., Lampe, Paul D.

Loss of connexin expression/gap junction intercellular communication (GJIC) has been correlated with decreased growth control and increased tumorigenesis. Studies utilizing Connexin32...

Mice deficient for the gap junction protein connexin32 exhibit increased radiation-induced tumorigenesis associated with elevated mitogen-activated protein kinase (p44/Erk1, p42/Erk2) activation (2004)

King, Timothy J., Lampe, Paul D.

Loss of connexin expression/gap junction intercellular communication (GJIC) has been correlated with decreased growth control and increased tumorigenesis. Studies utilizing Connexin32...

Enhanced myocyte contractility and Ca2+ handling in a calcineurin transgenic model of heart failure (2002)

Chu, Guoxiang, Carr, Andrew N., Young, Karen B., Lester, J.William, Yatani, Atsuko, Sanbe, Atsushi, ...

Objective: Impaired myocyte Ca2+ handling is a common characteristic of failing hearts and increases in calcineurin activity, a Ca2+-sensitive phosphatase, have been implicated in heart failure...

Clustering of connexin 43–enhanced green fluorescent protein gap junction channels and functional coupling in living cells

Bukauskas, Feliksas F., Jordan, Karen, Bukauskiene, Angele, Bennett, Michael V. L., Lampe, Paul D., Laird, Dale W., ...

Communication-incompetent cell lines were transfected with connexin (Cx) 43 fused with enhanced green fluorescent protein (EGFP) to examine the relation between Cx distribution determined by...

Trafficking, Assembly, and Function of a Connexin43-Green Fluorescent Protein Chimera in Live Mammalian CellsV⃞

Jordan, Karen, Solan, Joell L., Dominguez, Michel, Sia, Michael, Hand, Art, Lampe, Paul D., ...

To examine the trafficking, assembly, and turnover of connexin43 (Cx43) in living cells, we used an enhanced red-shifted mutant of green fluorescent protein (GFP) to construct a Cx43-GFP chimera....

Clustering of connexin 43–enhanced green fluorescent protein gap junction channels and functional coupling in living cells

Bukauskas, Feliksas F., Jordan, Karen, Bukauskiene, Angele, Bennett, Michael V. L., Lampe, Paul D., Laird, Dale W., ...

Communication-incompetent cell lines were transfected with connexin (Cx) 43 fused with enhanced green fluorescent protein (EGFP) to examine the relation between Cx distribution determined by...

Trafficking, Assembly, and Function of a Connexin43-Green Fluorescent Protein Chimera in Live Mammalian CellsV⃞

Jordan, Karen, Solan, Joell L., Dominguez, Michel, Sia, Michael, Hand, Art, Lampe, Paul D., ...

To examine the trafficking, assembly, and turnover of connexin43 (Cx43) in living cells, we used an enhanced red-shifted mutant of green fluorescent protein (GFP) to construct a Cx43-GFP chimera....

Protein kinase C spatially and temporally regulates gap junctional communication during human wound repair via phosphorylation of connexin43 on serine368

Richards, Theresa S., Dunn, Clarence A., Carter, William G., Usui, Marcia L., Olerud, John E., Lampe, Paul D.

Phosphorylation of connexin43 (Cx43) on serine368 (S368) has been shown to decrease gap junctional communication via a reduction in unitary channel conductance. Examination of phosphoserine368...

Cellular Interaction of Integrin α3β1 with Laminin 5 Promotes Gap Junctional Communication

Lampe, Paul D., Nguyen, Beth P., Gil, Susana, Usui, Marcia, Olerud, John, Takada, Yoshikazu, ...

Wounding of skin activates epidermal cell migration over exposed dermal collagen and fibronectin and over laminin 5 secreted into the provisional basement membrane. Gap junctional intercellular...

Phosphorylation of Connexin43 on Serine368 by Protein Kinase C Regulates Gap Junctional Communication

Lampe, Paul D., TenBroek, Erica M., Burt, Janis M., Kurata, Wendy E., Johnson, Ross G., Lau, Alan F.

Phorbol esters (e.g., TPA) activate protein kinase C (PKC), increase connexin43 (Cx43) phosphorylation, and decrease cell–cell communication via gap junctions in many cell types. We asked whether...

Ser364 of connexin43 and the upregulation of gap junction assembly by cAMP

TenBroek, Erica M., Lampe, Paul D., Solan, Joell L., Reynhout, James K., Johnson, Ross G.

The assembly of gap junctions (GJs) is a process coordinated by growth factors, kinases, and other signaling molecules. GJ assembly can be enhanced via the elevation of cAMP and subsequent...

Phosphorylation at S365 is a gatekeeper event that changes the structure of Cx43 and prevents down-regulation by PKC

Solan, Joell L., Marquez-Rosado, Lucrecia, Sorgen, Paul L., Thornton, Perry J., Gafken, Philip R., Lampe, Paul D.

Phosphorylation at unspecified sites is known to regulate the life cycle (assembly, gating, and turnover) of the gap junction protein, Cx43. In this paper, we show that Cx43 is phosphorylated on S365...

Gap junction remodeling and cardiac arrhythmogenesis in a murine model of oculodentodigital dysplasia

Kalcheva, Nellie, Qu, Jiaxiang, Sandeep, Nefthi, Garcia, Luis, Zhang, Jie, Wang, Zhiyong, ...

Gap junction channels are required for normal cardiac impulse propagation, and gap junction remodeling is associated with enhanced arrhythmic risk. Oculodentodigital dysplasia (ODDD) is a multisystem...

Impact of Freeze-thaw Cycles and Storage Time on Plasma Samples Used in Mass Spectrometry Based Biomarker Discovery Projects

Mitchell, Breeana L, Yasui, Yutaka, Li, Christopher I, Fitzpatrick, Annette L., Lampe, Paul D

Mass spectrometry approaches to biomarker discovery in human fluids have received a great deal of attention in recent years. While mass spectrometry instrumentation and analysis approaches have been...