Hamrick, Terri S., Harris, Sandra L., Spears, Patricia A., Havell, Edward A., Horton, John R., Russell, Perry W., ...
Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized. FimH is a minor component of type 1 pili that is...
Shelton, Celia B., Crosslin, David R., Casey, Jennifer L., Ng, S., Temple, Louise M., Orndorff, Paul E.
We discovered and characterized a temperate transducing bacteriophage (Ba1) for the avian respiratory pathogen Bordetella avium. Ba1 was initially identified along with one other phage (Ba2)...
Characterization of Escherichia coli Type 1 Pilus Mutants with Altered Binding Specificities
Harris, Sandra L., Spears, Patricia A., Havell, Edward A., Hamrick, Terri S., Horton, John R., Orndorff, Paul E.
PCR mutagenesis and a unique enrichment scheme were used to obtain two mutants, each with a single lesion in fimH, the chromosomal gene that encodes the adhesin protein (FimH) of Escherichia coli...
Immune Cells Are Required for Cutaneous Ulceration in a Swine Model of Chancroid
San Mateo, Lani R., Toffer, Kristen L., Orndorff, Paul E., Kawula, Thomas H.
Cutaneous lesions of the human sexually transmitted genital ulcer disease chancroid are characterized by the presence of intraepidermal pustules, keratinocyte cytopathology, and epidermal and dermal...
San Mateo, Lani R., Toffer, Kristen L., Orndorff, Paul E., Kawula, Thomas H.
Haemophilus ducreyi causes chancroid, a sexually transmitted cutaneous genital ulcer disease associated with increased heterosexual transmission of human immunodeficiency virus. H. ducreyi expresses...
Hamrick, Terri S., Havell, Edward A., Horton, John R., Orndorff, Paul E.
In an effort to better understand genetic and cellular factors that influence innate immunity, we examined host and bacterial factors involved in the nonopsonic phagocytosis and killing of...
Bordetella avium Virulence Measured In Vivo and In Vitro
Temple, Louise M., Weiss, Alison A., Walker, Kimberly E., Barnes, H. John, Christensen, Vern L., Miyamoto, David M., ...
Bordetella avium causes an upper-respiratory-tract disease called bordetellosis in birds. Bordetellosis shares many of the clinical and histopathological features of disease caused in mammals by...
Use of Bacteriophage Ba1 To Identify Properties Associated with Bordetella avium Virulence
Shelton, Celia B., Temple, Louise M., Orndorff, Paul E.
Bordetella avium causes bordetellosis, an upper respiratory disease of birds. Commercially raised turkeys are particularly susceptible. We report here on the use of a recently described B. avium...
Murphy, Erin R., Sacco, Randy E., Dickenson, Amy, Metzger, Daniel J., Hu, Yan, Orndorff, Paul E., ...
Iron (Fe) is an essential element for most organisms which must be obtained from the local environment. In the case of pathogenic bacteria, this fundamental element must be acquired from the fluids...
Influence of Extracellular Bactericidal Agents on Bacteria within Macrophages
Hamrick, Terri S., Diaz, Adam H., Havell, Edward A., Horton, John R., Orndorff, Paul E.
We employed gentamicin-sensitive and -resistant derivatives of Escherichia coli in a macrophage phagocytosis assay that compared λ bacteriophage and gentamicin as extracellular bactericidal agents....
Unexpected Similarities between Bordetella avium and Other Pathogenic Bordetellae
Spears, Patricia A., Temple, Louise M., Miyamoto, David M., Maskell, Duncan J., Orndorff, Paul E.
Bordetella avium causes an upper respiratory tract disease (bordetellosis) in avian species. Commercially raised turkeys are particularly susceptible. Like other pathogenic members of the genus...
The Product of the fimI Gene Is Necessary for Escherichia coli Type 1 Pilus Biosynthesis
Valenski, Mary L., Harris, Sandra L., Spears, Patricia A., Horton, John R., Orndorff, Paul E.
Site-directed mutagenesis was employed to create lesions in fimI, a gene of uncertain function located in the chromosomal gene cluster (fim) involved in Escherichia coli type 1 pilus biosynthesis....
Influence of Pregnancy on the Pathogenesis of Listeriosis in Mice Inoculated Intragastrically
Hamrick, Terri S., Horton, John R., Spears, Patricia A., Havell, Edward A., Smoak, Ida W., Orndorff, Paul E.
Pregnancy increases the risk of listeriosis, a systemic disease caused by Listeria monocytogenes. However, there is incomplete agreement on the reasons for this increased risk. We examined two...
A Humoral Immune Response Confers Protection against Haemophilus ducreyi Infection
Cole, Leah E., Toffer, Kristen L., Fulcher, Robert A., San Mateo, Lani R., Orndorff, Paul E., Kawula, Thomas H.
Haemophilus ducreyi is the etiologic agent of the sexually transmitted genital ulcer disease chancroid. Neither naturally occurring chancroid nor experimental infection with H. ducreyi results in...
Orndorff, Paul E., Devapali, Aditya, Palestrant, Sarah, Wyse, Aaron, Everett, Mary Lou, Bollinger, R. Randal, ...
The binding of human secretory immunoglobulin A (SIgA), the primary immunoglobulin in the gut, to Escherichia coli is thought to be dependent on type 1 pili. Type 1 pili are filamentous bacterial...
Afonina, Galyna, Leduc, Isabelle, Nepluev, Igor, Jeter, Chrystina, Routh, Patty, Almond, Glen, ...
The etiologic agent of chancroid is Haemophilus ducreyi. To fulfill its obligate requirement for heme, H. ducreyi uses two TonB-dependent receptors: the hemoglobin receptor (HgbA) and a receptor for...
Fulcher, Robert A., Cole, Leah E., Janowicz, Diane M., Toffer, Kristen L., Fortney, Kate R., Katz, Barry P., ...
Haemophilus ducreyi, the etiologic agent of the sexually transmitted genital ulcer disease chancroid, has been shown to associate with dermal collagen fibers within infected skin lesions. Here we...
Sebaihia, Mohammed, Preston, Andrew, Maskell, Duncan J., Kuzmiak, Holly, Connell, Terry D., King, Natalie D., ...
Bordetella avium is a pathogen of poultry and is phylogenetically distinct from Bordetella bronchiseptica, Bordetella pertussis, and Bordetella parapertussis, which are other species in the...
Hamrick, Terri S., Harris, Sandra L., Spears, Patricia A., Havell, Edward A., Horton, John R., Russell, Perry W., ...
Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized. FimH is a minor component of type 1 pili that is...
Shelton, Celia B., Crosslin, David R., Casey, Jennifer L., Ng, S., Temple, Louise M., Orndorff, Paul E.
We discovered and characterized a temperate transducing bacteriophage (Ba1) for the avian respiratory pathogen Bordetella avium. Ba1 was initially identified along with one other phage (Ba2)...
Characterization of Escherichia coli Type 1 Pilus Mutants with Altered Binding Specificities
Harris, Sandra L., Spears, Patricia A., Havell, Edward A., Hamrick, Terri S., Horton, John R., Orndorff, Paul E.
PCR mutagenesis and a unique enrichment scheme were used to obtain two mutants, each with a single lesion in fimH, the chromosomal gene that encodes the adhesin protein (FimH) of Escherichia coli...
Immune Cells Are Required for Cutaneous Ulceration in a Swine Model of Chancroid
San Mateo, Lani R., Toffer, Kristen L., Orndorff, Paul E., Kawula, Thomas H.
Cutaneous lesions of the human sexually transmitted genital ulcer disease chancroid are characterized by the presence of intraepidermal pustules, keratinocyte cytopathology, and epidermal and dermal...
San Mateo, Lani R., Toffer, Kristen L., Orndorff, Paul E., Kawula, Thomas H.
Haemophilus ducreyi causes chancroid, a sexually transmitted cutaneous genital ulcer disease associated with increased heterosexual transmission of human immunodeficiency virus. H. ducreyi expresses...
Hamrick, Terri S., Havell, Edward A., Horton, John R., Orndorff, Paul E.
In an effort to better understand genetic and cellular factors that influence innate immunity, we examined host and bacterial factors involved in the nonopsonic phagocytosis and killing of...
Bordetella avium Virulence Measured In Vivo and In Vitro
Temple, Louise M., Weiss, Alison A., Walker, Kimberly E., Barnes, H. John, Christensen, Vern L., Miyamoto, David M., ...
Bordetella avium causes an upper-respiratory-tract disease called bordetellosis in birds. Bordetellosis shares many of the clinical and histopathological features of disease caused in mammals by...
Use of Bacteriophage Ba1 To Identify Properties Associated with Bordetella avium Virulence
Shelton, Celia B., Temple, Louise M., Orndorff, Paul E.
Bordetella avium causes bordetellosis, an upper respiratory disease of birds. Commercially raised turkeys are particularly susceptible. We report here on the use of a recently described B. avium...
Murphy, Erin R., Sacco, Randy E., Dickenson, Amy, Metzger, Daniel J., Hu, Yan, Orndorff, Paul E., ...
Iron (Fe) is an essential element for most organisms which must be obtained from the local environment. In the case of pathogenic bacteria, this fundamental element must be acquired from the fluids...
Influence of Extracellular Bactericidal Agents on Bacteria within Macrophages
Hamrick, Terri S., Diaz, Adam H., Havell, Edward A., Horton, John R., Orndorff, Paul E.
We employed gentamicin-sensitive and -resistant derivatives of Escherichia coli in a macrophage phagocytosis assay that compared λ bacteriophage and gentamicin as extracellular bactericidal agents....
Unexpected Similarities between Bordetella avium and Other Pathogenic Bordetellae
Spears, Patricia A., Temple, Louise M., Miyamoto, David M., Maskell, Duncan J., Orndorff, Paul E.
Bordetella avium causes an upper respiratory tract disease (bordetellosis) in avian species. Commercially raised turkeys are particularly susceptible. Like other pathogenic members of the genus...
The Product of the fimI Gene Is Necessary for Escherichia coli Type 1 Pilus Biosynthesis
Valenski, Mary L., Harris, Sandra L., Spears, Patricia A., Horton, John R., Orndorff, Paul E.
Site-directed mutagenesis was employed to create lesions in fimI, a gene of uncertain function located in the chromosomal gene cluster (fim) involved in Escherichia coli type 1 pilus biosynthesis....
Influence of Pregnancy on the Pathogenesis of Listeriosis in Mice Inoculated Intragastrically
Hamrick, Terri S., Horton, John R., Spears, Patricia A., Havell, Edward A., Smoak, Ida W., Orndorff, Paul E.
Pregnancy increases the risk of listeriosis, a systemic disease caused by Listeria monocytogenes. However, there is incomplete agreement on the reasons for this increased risk. We examined two...
A Humoral Immune Response Confers Protection against Haemophilus ducreyi Infection
Cole, Leah E., Toffer, Kristen L., Fulcher, Robert A., San Mateo, Lani R., Orndorff, Paul E., Kawula, Thomas H.
Haemophilus ducreyi is the etiologic agent of the sexually transmitted genital ulcer disease chancroid. Neither naturally occurring chancroid nor experimental infection with H. ducreyi results in...
Orndorff, Paul E., Devapali, Aditya, Palestrant, Sarah, Wyse, Aaron, Everett, Mary Lou, Bollinger, R. Randal, ...
The binding of human secretory immunoglobulin A (SIgA), the primary immunoglobulin in the gut, to Escherichia coli is thought to be dependent on type 1 pili. Type 1 pili are filamentous bacterial...
Afonina, Galyna, Leduc, Isabelle, Nepluev, Igor, Jeter, Chrystina, Routh, Patty, Almond, Glen, ...
The etiologic agent of chancroid is Haemophilus ducreyi. To fulfill its obligate requirement for heme, H. ducreyi uses two TonB-dependent receptors: the hemoglobin receptor (HgbA) and a receptor for...
Fulcher, Robert A., Cole, Leah E., Janowicz, Diane M., Toffer, Kristen L., Fortney, Kate R., Katz, Barry P., ...
Haemophilus ducreyi, the etiologic agent of the sexually transmitted genital ulcer disease chancroid, has been shown to associate with dermal collagen fibers within infected skin lesions. Here we...
Sebaihia, Mohammed, Preston, Andrew, Maskell, Duncan J., Kuzmiak, Holly, Connell, Terry D., King, Natalie D., ...
Bordetella avium is a pathogen of poultry and is phylogenetically distinct from Bordetella bronchiseptica, Bordetella pertussis, and Bordetella parapertussis, which are other species in the...