Live cell imaging of repetitive DNA sequences via GFP-tagged polydactyl zinc finger proteins (2007)
Lindhout, Beatrice I., Fransz, Paul, Tessadori, Federico, Meckel, Tobias, Hooykaas, Paul J.J., Van Der Zaal, Bert J.
Several techniques are available to study chromosomes or chromosomal domains in nuclei of chemically fixed or living cells. Current methods to detect DNA sequences in vivo are limited to trans...
Van Attikum, Haico, Hooykaas, Paul J. J.
Agrobacterium tumefaciens delivers transferred DNA (T‐DNA) into cells of plants and yeast. In plants, the T‐DNA integrates at random positions into the genome by non‐homologous...
Schrammeijer, Barbara, Dulk-Ras, Amke Den, Vergunst, Annette C., Jurado Jácome, Esmeralda, Hooykaas, Paul J. J.
Agrobacterium tumefaciens causes crown gall disease on a variety of plants. During the infection process Agrobacterium transfers a nucleoprotein complex, the VirD2 T‐complex, and at least two...
Van Attikum, Haico, Bundock, Paul, Overmeer, René M., Lee, Lan-Ying, Gelvin, Stanton B., Hooykaas, Paul J. J.
The joining of breaks in the chromosomal DNA backbone by ligases in processes of replication, recombination and repair plays a crucial role in the maintenance of genomic stability. Four...
Neuteboom, Leon W., Veth-Tello, Luz M., Clijdesdale, Olton R., Hooykaas, Paul J. J., Van Der Zaal, Bert J.
Differential screening of a cDNA library for mRNA species that specifically accumulate during auxin-induced lateral root formation in Arabidopsis thaliana led to the isolation of the AIR3 cDNA clone....
Analysis of the complete nucleotide sequence of the Agrobacterium tumefaciens virB operon (1988)
Thompson, David V., Melchers, Leo S., Idler, Ken B., Schilperoort, Rob A., Hooykaas, Paul J.J.
The complete nucleotide sequence of the virB locus, from the octopine Ti plasmid of Agrobacterium tumefaciens strain 15955, has been determined. In the large virB-operon (9600 nucleotides) we have...
Van Der Zaal, Bert J., Neuteboom, Leon W., Pinas, Johan E., Chardonnens, Agnes N., Schat, Henk, Verkleij, Jos A.C., ...
We describe the isolation of an Arabidopsis gene that is closely related to the animal ZnT genes (Zn transporter). The protein encoded by the ZAT (Zn transporter of Arabidopsis thaliana) gene has 398...
The Bases of Crown Gall Tumorigenesis
Zhu, Jun, Oger, Philippe M., Schrammeijer, Barbara, Hooykaas, Paul J. J., Farrand, Stephen K., Winans, Stephen C.
Non-homologous end-joining proteins are required for Agrobacterium T-DNA integration
Van Attikum, Haico, Bundock, Paul, Hooykaas, Paul J.J.
Agrobacterium tumefaciens causes crown gall disease in dicotyledonous plants by introducing a segment of DNA (T-DNA), derived from its tumour-inducing (Ti) plasmid, into plant cells at infection...
Schrammeijer, Barbara, Vergunst, Annette C., Jurado Jácome, Esmeralda, Hooykaas, Paul J. J.
Agrobacterium tumefaciens causes crown gall disease on a variety of plants. During the infection process Agrobacterium transfers a nucleoprotein complex, the VirD2 T-complex, and at least two Vir...
Genetic requirements for the targeted integration of Agrobacterium T-DNA in Saccharomyces cerevisiae
Van Attikum, Haico, Hooykaas, Paul J. J.
Agrobacterium tumefaciens delivers transferred DNA (T-DNA) into cells of plants and yeast. In plants, the T-DNA integrates at random positions into the genome by non-homologous recombination (NHR),...
Van Attikum, Haico, Bundock, Paul, Overmeer, René M., Lee, Lan-Ying, Gelvin, Stanton B., Hooykaas, Paul J. J.
The joining of breaks in the chromosomal DNA backbone by ligases in processes of replication, recombination and repair plays a crucial role in the maintenance of genomic stability. Four ATP-dependent...
PINOID-Mediated Signaling Involves Calcium-Binding Proteins
Benjamins, René, Ampudia, Carlos S. Galván, Hooykaas, Paul J.J., Offringa, Remko
The plant hormone auxin is a central regulator of plant development. In Arabidopsis, the PINOID (PID) protein serine/threonine kinase is a key component in the signaling of this phytohormone. To...
Vergunst, Annette C., Hooykaas, Paul J.J.
Agrobacterium tumefaciens uses a type IV secretion system to deliver a nucleoprotein complex and effector proteins directly into plant cells. The single-stranded DNA-binding protein VirE2, the F-box...
Weijers, Dolf, Van Hamburg, Jan-Piet, Van Rijn, Erwin, Hooykaas, Paul J.J., Offringa, Remko
Fertilization of the female gametophyte in angiosperm plants initiates a process of coordinated development of embryo, endosperm, and seed coat that ensures the production of a viable seed. Mutant...
Michielse, Caroline B., Ram, Arthur F. J., Hooykaas, Paul J. J.
Reductions to 2, 5, and 42% of the wild-type transformation efficiency were found when Agrobacterium mutants carrying transposon insertions in virD2, virC2, and virE2, respectively, were used to...
Vergunst, Annette C., Grosse Stüve, Thomas A., Ouwehand, Anette, Hooykaas, Paul J. J.
Several human pathogens and the plant pathogen Agrobacterium tumefaciens use a type IV secretion system for translocation of effector proteins into host cells. How effector proteins are selected for...
Van Der Zaal, Bert J., Neuteboom, Leon W., Pinas, Johan E., Chardonnens, Agnes N., Schat, Henk, Verkleij, Jos A.C., ...
We describe the isolation of an Arabidopsis gene that is closely related to the animal ZnT genes (Zn transporter). The protein encoded by the ZAT (Zn transporter of Arabidopsis thaliana) gene has 398...
The Bases of Crown Gall Tumorigenesis
Zhu, Jun, Oger, Philippe M., Schrammeijer, Barbara, Hooykaas, Paul J. J., Farrand, Stephen K., Winans, Stephen C.
Non-homologous end-joining proteins are required for Agrobacterium T-DNA integration
Van Attikum, Haico, Bundock, Paul, Hooykaas, Paul J.J.
Agrobacterium tumefaciens causes crown gall disease in dicotyledonous plants by introducing a segment of DNA (T-DNA), derived from its tumour-inducing (Ti) plasmid, into plant cells at infection...
Schrammeijer, Barbara, Vergunst, Annette C., Jurado Jácome, Esmeralda, Hooykaas, Paul J. J.
Agrobacterium tumefaciens causes crown gall disease on a variety of plants. During the infection process Agrobacterium transfers a nucleoprotein complex, the VirD2 T-complex, and at least two Vir...
Genetic requirements for the targeted integration of Agrobacterium T-DNA in Saccharomyces cerevisiae
Van Attikum, Haico, Hooykaas, Paul J. J.
Agrobacterium tumefaciens delivers transferred DNA (T-DNA) into cells of plants and yeast. In plants, the T-DNA integrates at random positions into the genome by non-homologous recombination (NHR),...
Van Attikum, Haico, Bundock, Paul, Overmeer, René M., Lee, Lan-Ying, Gelvin, Stanton B., Hooykaas, Paul J. J.
The joining of breaks in the chromosomal DNA backbone by ligases in processes of replication, recombination and repair plays a crucial role in the maintenance of genomic stability. Four ATP-dependent...
PINOID-Mediated Signaling Involves Calcium-Binding Proteins
Benjamins, René, Ampudia, Carlos S. Galván, Hooykaas, Paul J.J., Offringa, Remko
The plant hormone auxin is a central regulator of plant development. In Arabidopsis, the PINOID (PID) protein serine/threonine kinase is a key component in the signaling of this phytohormone. To...
Vergunst, Annette C., Hooykaas, Paul J.J.
Agrobacterium tumefaciens uses a type IV secretion system to deliver a nucleoprotein complex and effector proteins directly into plant cells. The single-stranded DNA-binding protein VirE2, the F-box...
Weijers, Dolf, Van Hamburg, Jan-Piet, Van Rijn, Erwin, Hooykaas, Paul J.J., Offringa, Remko
Fertilization of the female gametophyte in angiosperm plants initiates a process of coordinated development of embryo, endosperm, and seed coat that ensures the production of a viable seed. Mutant...
Michielse, Caroline B., Ram, Arthur F. J., Hooykaas, Paul J. J.
Reductions to 2, 5, and 42% of the wild-type transformation efficiency were found when Agrobacterium mutants carrying transposon insertions in virD2, virC2, and virE2, respectively, were used to...
Vergunst, Annette C., Grosse Stüve, Thomas A., Ouwehand, Anette, Hooykaas, Paul J. J.
Several human pathogens and the plant pathogen Agrobacterium tumefaciens use a type IV secretion system for translocation of effector proteins into host cells. How effector proteins are selected for...
The Agrobacterium VirE3 effector protein: a potential plant transcriptional activator
García-Rodríguez, Fernando M., Schrammeijer, Barbara, Hooykaas, Paul J. J.
During the infection of plants, Agrobacterium tumefaciens introduces several Virulence proteins including VirE2, VirF, VirD5 and VirE3 into plant cells in addition to the T-DNA. Here, we report that...
Hodges, Larry D., Vergunst, Annette C., Neal-McKinney, Jason, Moyer, Deborah M., Hooykaas, Paul J. J., ...
Agrobacterium tumefaciens and Agrobacterium rhizogenes are closely related plant pathogens that cause different diseases, crown gall and hairy root. Both diseases result from transfer, integration,...
Live cell imaging of repetitive DNA sequences via GFP-tagged polydactyl zinc finger proteins
Lindhout, Beatrice I., Fransz, Paul, Tessadori, Federico, Meckel, Tobias, Hooykaas, Paul J.J., Van Der Zaal, Bert J.
Several techniques are available to study chromosomes or chromosomal domains in nuclei of chemically fixed or living cells. Current methods to detect DNA sequences in vivo are limited to trans...
Stable Recombinase-Mediated Cassette Exchange in Arabidopsis Using Agrobacterium tumefaciens1
Louwerse, Jeanine D., Van Der Steen, Dirk M., Hooykaas, Paul J.J., Vergunst, Annette C.
Site-specific integration is an attractive method for the improvement of current transformation technologies aimed at the production of stable transgenic plants. Here, we present a Cre-based...