Paul L. Greenhaff

Modulation of extracellular matrix genes reflects the magnitude of physiological adaptation to aerobic exercise training in humans (2005)

Timmons, James A, Jansson, Eva, Fischer, Helene, Gustafsson, Thomas, Greenhaff, Paul L, Ridden, John, ...

Abstract Background Regular exercise reduces cardiovascular and metabolic disease partly through improved aerobic fitness. The determinants of exercise-induced gains in aerobic fitness in humans are...

Gender and age differences in plasma carnitine, muscle strength, and exercise tolerance in haemodialysis patients (2002)

Constantin-Teodosiu, Dumitru, Young, Stephanie, Wellock, Fiona, Short, Anthony H., Burden, Richard P., Morgan, Anthony G., ...

Background. Haemodialysis patients have impaired exercise tolerance that may be related to impaired carnitine availability and/or alterations in carnitine metabolism. As carnitine metabolism is...

Increased uncoupling protein 3 content does not affect mitochondrial function in human skeletal muscle in vivo

Hesselink, Matthijs K.C., Greenhaff, Paul L., Constantin-Teodosiu, Dimitru, Hultman, Eric, Saris, Wim H.M., Nieuwlaat, Robby, ...

Phosphocreatine (PCr) resynthesis rate following intense anoxic contraction can be used as a sensitive index of in vivo mitochondrial function. We examined the effect of a diet-induced increase in...

Modulation of extracellular matrix genes reflects the magnitude of physiological adaptation to aerobic exercise training in humans

Timmons, James A, Jansson, Eva, Fischer, Helene, Gustafsson, Thomas, Greenhaff, Paul L, Ridden, John, ...

An acute decrease in TCA cycle intermediates does not affect aerobic energy delivery in contracting rat skeletal muscle

Dawson, Kristen D, Baker, David J, Greenhaff, Paul L, Gibala, Martin J

We tested the hypothesis that an acute decrease in muscle TCA cycle intermediates during contraction would compromise aerobic energy delivery. Male Wistar rats were anaesthetized and the...

Muscle pyruvate availability can limit the flux, but not activation, of the pyruvate dehydrogenase complex during submaximal exercise in humans

Constantin-Teodosiu, Dumitru, Peirce, Nicholas S, Fox, John, Greenhaff, Paul L

While lowering muscle glycogen availability to an extent that would reduce muscle pyruvate formation during intense exercise, we investigated the importance of muscle pyruvate availability to...

Acetyl group availability influences phosphocreatine degradation even during intense muscle contraction

Timmons, James A, Constantin-Teodosiu, Dumitru, Poucher, Simon M, Greenhaff, Paul L

We previously established that activation of the pyruvate dehydrogenase complex (PDC) using dichloroacetate (DCA) reduced the reliance on substrate-level phosphorylation (SLP) at the onset of...

Increased uncoupling protein 3 content does not affect mitochondrial function in human skeletal muscle in vivo

Hesselink, Matthijs K.C., Greenhaff, Paul L., Constantin-Teodosiu, Dimitru, Hultman, Eric, Saris, Wim H.M., Nieuwlaat, Robby, ...

Phosphocreatine (PCr) resynthesis rate following intense anoxic contraction can be used as a sensitive index of in vivo mitochondrial function. We examined the effect of a diet-induced increase in...

Modulation of extracellular matrix genes reflects the magnitude of physiological adaptation to aerobic exercise training in humans

Timmons, James A, Jansson, Eva, Fischer, Helene, Gustafsson, Thomas, Greenhaff, Paul L, Ridden, John, ...

An acute decrease in TCA cycle intermediates does not affect aerobic energy delivery in contracting rat skeletal muscle

Dawson, Kristen D, Baker, David J, Greenhaff, Paul L, Gibala, Martin J

We tested the hypothesis that an acute decrease in muscle TCA cycle intermediates during contraction would compromise aerobic energy delivery. Male Wistar rats were anaesthetized and the...

Muscle pyruvate availability can limit the flux, but not activation, of the pyruvate dehydrogenase complex during submaximal exercise in humans

Constantin-Teodosiu, Dumitru, Peirce, Nicholas S, Fox, John, Greenhaff, Paul L

While lowering muscle glycogen availability to an extent that would reduce muscle pyruvate formation during intense exercise, we investigated the importance of muscle pyruvate availability to...

Acetyl group availability influences phosphocreatine degradation even during intense muscle contraction

Timmons, James A, Constantin-Teodosiu, Dumitru, Poucher, Simon M, Greenhaff, Paul L

We previously established that activation of the pyruvate dehydrogenase complex (PDC) using dichloroacetate (DCA) reduced the reliance on substrate-level phosphorylation (SLP) at the onset of...

Dual effects of dichloroacetate on cardiac ischaemic preconditioning in the rat isolated perfused heart

Randall, Michael D, Keon, Claudia A, Greenhaff, Paul L, Constantin-Teodosiu, Dumitru

Ischaemic cardiac preconditioning represents an important cardioprotective mechanism which limits myocardial ischaemic damage. The aim of this investigation was to assess the impact of...

The effects of increasing exercise intensity on muscle fuel utilisation in humans

Greenhaff, Paul L, Constantin-Teodosiu, D, Saris, Wim H M, Wagenmakers, Anton J M

Contemporary stable isotope methodology was applied in combination with muscle biopsy sampling to accurately quantify substrate utilisation and study the regulation of muscle fuel selection during...

Oral creatine supplementation facilitates the rehabilitation of disuse atrophy and alters the expression of muscle myogenic factors in humans

Hespel, Peter, Op't Eijnde, Bert, Leemputte, Marc Van, Ursø, Birgitte, Greenhaff, Paul L, Labarque, Valery, ...

We investigated the effect of oral creatine supplementation during leg immobilization and rehabilitation on muscle volume and function, and on myogenic transcription factor expression in human...

The creatine-phosphocreatine system: there's more than one song in its repertoire

Greenhaff, Paul L

Oxygen uptake on-kinetics in dog gastrocnemius in situ following activation of pyruvate dehydrogenase by dichloroacetate

Grassi, Bruno, Hogan, Michael C, Greenhaff, Paul L, Hamann, Jason J, Kelley, Kevin M, Aschenbach, William G, ...

The aim of the present study was to determine whether the activation of the pyruvate dehydrogenase complex (PDC) by dichloroacetate (DCA) is associated with faster O2 uptake (V̇O2) on-kinetics....

Low intensity exercise in humans accelerates mitochondrial ATP production and pulmonary oxygen kinetics during subsequent more intense exercise

Campbell-O'Sullivan, Síun P, Constantin-Teodosiu, Dumitru, Peirce, Nicholas, Greenhaff, Paul L

We undertook this study to determine whether low intensity exercise (55 % V̇O2,max) would significantly alter the metabolic and ventilatory responses observed during 10 min of subsequent moderate...

Exercise with low muscle glycogen augments TCA cycle anaplerosis but impairs oxidative energy provision in humans

Gibala, Martin J, Peirce, Nick, Constantin-Teodosiu, Dimitru, Greenhaff, Paul L

We tested the hypotheses that: (i) exercise with low muscle glycogen would reduce pyruvate flux through the alanine aminotransferase (AAT) reaction and attenuate the increase in tricarboxylic acid...

The acetyl group deficit at the onset of contraction in ischaemic canine skeletal muscle

Roberts, Paul A, Loxham, Susan J G, Poucher, Simon M, Constantin-Teodosiu, Dumitru, Greenhaff, Paul L

Considerable debate surrounds the identity of the precise cellular site(s) of inertia that limit the contribution of mitochondrial ATP resynthesis towards a step increase in workload at the onset of...

The temporal relationship between glycogen phosphorylase and activation of the pyruvate dehydrogenase complex during adrenaline infusion in resting canine skeletal muscle

Roberts, Paul A, Loxham, Susan J G, Poucher, Simon M, Constantin-Teodosiu, Dumitru, Greenhaff, Paul L

The present study examined the effect of adrenaline infusion on the activation status of glycogen phosphorylase and the pyruvate dehydrogenase complex (PDC) and on the accumulation of...

Milestones in human physiology: muscle energy metabolism and blood flow during contraction

Greenhaff, Paul L

New insights concerning the role of carnitine in the regulation of fuel metabolism in skeletal muscle

Stephens, Francis B, Constantin-Teodosiu, Dumitru, Greenhaff, Paul L

In skeletal muscle, carnitine plays an essential role in the translocation of long-chain fatty-acids into the mitochondrial matrix for subsequent β-oxidation, and in the regulation of the...

PPARδ agonism induces a change in fuel metabolism and activation of an atrophy programme, but does not impair mitochondrial function in rat skeletal muscle

Constantin, Despina, Constantin-Teodosiu, Dumitru, Layfield, Robert, Tsintzas, Kostas, Bennett, Andrew J, Greenhaff, Paul L

PPARα agonism impairs mitochondrial function, but the effect of PPARδ agonism on mitochondrial function is equivocal. Furthermore, PPARα and δ agonism increases muscle fatty acid oxidation,...

Effects of Endotoxaemia on Protein Metabolism in Rat Fast-Twitch Skeletal Muscle and Myocardium

Murton, Andrew J., Alamdari, Nima, Gardiner, Sheila M., Constantin-Teodosiu, Dumitru, Layfield, Robert, Bennett, Terence, ...

A potential role for Akt/FOXO signalling in both protein loss and the impairment of muscle carbohydrate oxidation during sepsis in rodent skeletal muscle

Crossland, Hannah, Constantin-Teodosiu, Dumitru, Gardiner, Sheila M, Constantin, Despina, Greenhaff, Paul L

Sepsis causes muscle atrophy and insulin resistance, but the underlying mechanisms are unclear. Therefore, the present study examined the effects of lipopolysaccharide (LPS)-induced endotoxaemia on...