Paul R. Young

Modular alpha-helical mimetics with antiviral activity against respiratory syncitial virus (2006)

Shepherd, Nicholas E., Hoang, Huy N., Desai, Vishal S., Letouze, Eric, Young, Paul R., Fairlie, David P.

A 13-residue peptide sequence from a respiratory syncitial virus fusion protein was constrained in an alpha-helical conformation by fusing two back-to-back cyclic alpha-turn mimetics. The resulting...

The role of histidine residues in low-pH-mediated viral membrane fusion (2006)

Kampmann,Thorsten, Meuller, Daniela S., Mark, Alan E., Young, Paul R., Kobe, Bostjan

A central event in the invasion of a host cell by an enveloped virus is the fusion of viral and cell membranes. For many viruses, membrane fusion is driven by specific viral surface proteins that...

Site-directed Mutagenesis and Kinetic Studies of the West Nile Virus NS3 Protease Identify Key Enzyme-Substrate Interactions (2005)

Keith J. Chappell, Tessa A. Nall, Martin J. Stoermer, Ning-Xia Fang, David P. Fairlie, ...

A series of papers that have been published since our 2001 paper (above) has built on our understanding of flavivirus protease specificity. We have expanded our studies to incorporate West Nile virus...

Site-directed Mutagenesis and Kinetic Studies of the West Nile Virus NS3 Protease Identify Key Enzyme-Substrate Interactions (2005)

Keith J. Chappell, Tessa A. Nall, Martin J. Stoermer, Ning-Xia Fang, David P. Fairlie, ...

A series of papers that have been published since our 2001 paper (above) has built on our understanding of flavivirus protease specificity. We have expanded our studies to incorporate West Nile virus...

Site-directed mutagenesis and kinetic studies of the West Nile virus NS3 protease identify key enzyme-substrate interactions (2005)

Chappell, Keith J., Nall, Tessa A., Stoermer, Martin J., Fang, Ning-Xia, Tyndall, Joel D. A., Fairlie, David P., ...

The flavivirus West Nile virus (WNV) has spread rapidly throughout the world in recent years causing fever, meningitis, encephalitis, and fatalities. Because the viral protease NS2B/NS3 is essential...

High Circulating Levels of the Dengue Virus Nonstructural Protein NS1 Early in Dengue Illness Correlate with the Development of Dengue Hemorrhagic Fever (2002)

Daniel H. Libraty, Paul R. Young, Darren Pickering, Timothy P. Endy, Siripen Kalayanarooj, Sharone Green, ...

This paper builds clinical experience onto our earlier development and publication (Young et al, Journal of Clinical Microbiology, 2000) of an antigen capture assay for dengue diagnosis. The assay...

Properties of Selected S-Nitrosothiols Compared to Nitrosylated WR-1065 (2002)

William Michael Whiteside, Devin N. Sears, Paul R. Young, David B. Rubin

Whiteside, W. M., Sears, D. N., Young, P. R. and Rubin, D. B. Properties of Selected S-Nitrosothiols Compared to Nitrosylated WR-1065. Radiat. Res. 157, 578–588 (2002). WR-1065...

High Circulating Levels of the Dengue Virus Nonstructural Protein NS1 Early in Dengue Illness Correlate with the Development of Dengue Hemorrhagic Fever (2002)

Daniel H. Libraty, Paul R. Young, Darren Pickering, Timothy P. Endy, Siripen Kalayanarooj, Sharone Green, ...

This paper builds clinical experience onto our earlier development and publication (Young et al, Journal of Clinical Microbiology, 2000) of an antigen capture assay for dengue diagnosis. The assay...

Activity of Recombinant Dengue 2 Virus NS3 Protease in the Presence of a Truncated NS2B Co-factor, Small Peptide Substrates, and Inhibitors (2001)

Donmienne Leung, Kate Schroder, Helen White, Ning-Xia Fang, Martin J. Stoermer, Giovanni Abbenante, ...

Our paper describes the expression and purification of the first active recombinant flavivirus protease suitable for in vitro assays. We used this assay to probe substrate specificity and develop the...

Basic Mass Spectroscopy (2001)

Young, Paul R.

This site presents material on fragmentation and the interpretation of mass spectra. This site also contains problems consisting of the mass spectra of 5 unknowns along with solutions for the...

An Antigen Capture Enzyme-Linked Immunosorbent Assay Reveals High Levels of the Dengue Virus Protein NS1 in the Sera of Infected Patients

Young, Paul R., Hilditch, Paige A., Bletchly, Cheryl, Halloran, Wendy

We describe the development of a capture enzyme-linked immunosorbent assay for the detection of the dengue virus nonstructural protein NS1. The assay employs rabbit polyclonal and monoclonal...

Stable High-Level Expression of Heterologous Genes In Vitro and In Vivo by Noncytopathic DNA-Based Kunjin Virus Replicon Vectors†

Varnavski, Andrei N., Young, Paul R., Khromykh, Alexander A.

Primary features of the flavivirus Kunjin (KUN) subgenomic replicons include continuous noncytopathic replication in host cell cytoplasm and the ability to be encapsidated into secreted virus-like...

An Antigen Capture Enzyme-Linked Immunosorbent Assay Reveals High Levels of the Dengue Virus Protein NS1 in the Sera of Infected Patients

Young, Paul R., Hilditch, Paige A., Bletchly, Cheryl, Halloran, Wendy

We describe the development of a capture enzyme-linked immunosorbent assay for the detection of the dengue virus nonstructural protein NS1. The assay employs rabbit polyclonal and monoclonal...

Stable High-Level Expression of Heterologous Genes In Vitro and In Vivo by Noncytopathic DNA-Based Kunjin Virus Replicon Vectors†

Varnavski, Andrei N., Young, Paul R., Khromykh, Alexander A.

Primary features of the flavivirus Kunjin (KUN) subgenomic replicons include continuous noncytopathic replication in host cell cytoplasm and the ability to be encapsidated into secreted virus-like...

NMR Spectroscopy Tutorial

Paul R. Young

This is a straight forward elementary approach to proton and carbon NMR analysis targeted at students who are just at the most beginning stages of NMR spectroscopy, for example those in a...

Activity of Recombinant Dengue 2 Virus NS3 Protease in the Presence of a Truncated NS2B Co-factor, Small Peptide Substrates, and Inhibitors

Donmienne Leung, Kate Schroder, Helen White, Ning-Xia Fang, Martin J. Stoermer, Giovanni Abbenante, ...

Our paper describes the expression and purification of the first active recombinant flavivirus protease suitable for in vitro assays. We used this assay to probe substrate specificity and develop the...