Ritchie, Allyson V., Van Es, Saskia, Fouquet, Celine, Schaap, Pauline
Amoebas and other protists commonly encyst when faced with environmental stress. Although little is known of the signaling pathways that mediate encystation, the analogous process of spore formation...
The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase (2001)
Roelofs, Jeroen, Meima, Marcel, Schaap, Pauline
A new Dictyostelium discoideum cyclase gene was identified that encodes a protein (sGC) with 35% similarity to mammalian soluble adenylyl cyclase (sAC). Gene disruption of sGC has no effect on...
Kim, Ji-Yun, Soede, Ron D.M., Schaap, Pauline, Valkema, Romi, Borleis, Jane A., ...
In several G-protein-coupled signaling systems, ligand-induced receptor phosphorylation by specific kinases is suggested to lead to desensitization via mechanisms including receptor/G-protein...
Schaap, Pauline, Ments-Cohen, Martine Van, Soede, Ron D.M., Brandt, Raymond, Firtel, Richard A., Dostmann, Wolfgang, ...
A novel class of cAMP derivatives were tested for binding to surface cAMP receptors (CAR), protein kinase A (PKA), and cAMP-phosphodiesterase (PDE) and for induction of three classes of cAMP...
Peters, Dorien J.M., Snaar-Jagalska, B. Ewa, Schaap, Pauline
Li+ drastically alters pattern formation in Dictyostelium by inhibiting cAMP-induced prespore-gene expression and promoting cAMP-induced prestalk-gene expression. We reported previously that Li+...
Wang, Mei, Bominaar, Anthony A., Devreotes, Peter N., Schaap, Pauline
Surface cAMP receptors on Dictyostelium cells are linked to several second messenger systems and mediate multiple physiological responses, including chemotaxis and differentiation. Activation of the...
Ments-Cohen, Martine Van, Genieser, Hans-Gottfried, Jastorff, Bernd, Schaap, Pauline
Dictyostelium cells exhibit four types of kinetically distinct surface cAMP binding sites, the AH, AL, BS, and BSS sites, which are down-regulated during persistent stimulation with cAMP. Although...
Bominaar, Anthony A., Kesbeke, Fanja, Snaar-Jagalska, B. Ewa, Peters, Dorien J.M., Schaap, Pauline
Dictyostelium cells use extracellular cyclic AMP both as a chemoattractant and as a morphogen inducing cell-type-specific gene expression. Cyclic AMP binds to surface receptors, activates one or more...
Peters, Dorien J.M., Bominaar, Anthony A., Snaar-Jagalska, B. Ewa, Brandt, Raymond, Ceccarelli, Adriano, ...
During development of the cellular slime mold Dictyostelium discoideum, cAMP induces chemotaxis and expression of different classes of genes by means of interaction with surface cAMP receptors. We...
Peters, Dorien J.M., Spek, Wouter, Schaap, Pauline
We investigated the effect of Li+ on two types of cyclic AMP-regulated gene expression and on basal and cyclic AMP-stimulated inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) levels. Li+ effectively...
Wang, Mei, Devreotes, Peter N., Schaap, Pauline
cAMP chemoattractant receptors on the surface of Dictyostelium discoideum cells are visualized by means of immunocytochemistry. Receptor antigen is virtually absent from growing cells and begins to...
Schaap, Pauline, Driel, Roel Van, Spek, Wouter, Pinas, Johan
Cyclic AMP induces postaggregative differentiation in aggregation competent cells of Dictyostelium by interacting with cell surface cAMP receptors. We investigated the transduction pathway of this...
Adenosine acts as a cyclic AMP antagonist in Dictyostelium discoideum. It inhibits the binding of cyclic AMP to cell surface receptors and the induction of postaggregative differentiation by cyclic...
Kesbeke, Fanja, Schaap, Pauline
Cyclic AMP-induced cAMP and cGMP responses during development of Dictyostelium discoideum were investigated. The cAMP-induced cGMP response is maximal when aggregation is in full progress, and then...
Schaap, Pauline, Konijn, Theo M.
Aggregation in the primitive cellular slime mold Dictyostelium minutum proceeds by means of chemotaxis toward a continuously secreted folic acid analog. The onset of culmination is marked by the...
Functional Promiscuity of Gene Regulation by Serpentine Receptors in Dictyostelium discoideum
Kim, Ji-Yun, Brandt, Raymond, Devreotes, Peter N., Schaap, Pauline
Serpentine receptors such as smoothened and frizzled play important roles in cell fate determination during animal development. In Dictyostelium discoideum, four serpentine cyclic AMP (cAMP)...
The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase
Roelofs, Jeroen, Meima, Marcel, Schaap, Pauline
A new Dictyostelium discoideum cyclase gene was identified that encodes a protein (sGC) with 35% similarity to mammalian soluble adenylyl cyclase (sAC). Gene disruption of sGC has no effect on...
Meima, Marcel E., Biondi, Ricardo M., Schaap, Pauline
StmF mutants are chemotactic mutants that are defective in a cGMP phosphodiesterase (PDE) activity. We identified a novel gene, PdeD, that harbors two cyclic nucleotide–binding domains and a...
Schaap, Pauline, Konijn, Theo M.
Aggregation in the primitive cellular slime mold Dictyostelium minutum proceeds by means of chemotaxis toward a continuously secreted folic acid analog [De Wit, R. J. W. & Konijn, T. M. (1983) Cell...
Adenylyl Cyclase G Is Activated by an Intramolecular Osmosensor
Saran, Shweta, Schaap, Pauline
Adenylyl cyclase G (ACG) is activated by high osmolality and mediates inhibition of spore germination by this stress factor. The catalytic domains of all eukaryote cyclases are active as dimers and...
Evolutionary origin of cAMP-based chemoattraction in the social amoebae
Alvarez-Curto, Elisa, Rozen, Daniel E., Ritchie, Allyson V., Fouquet, Celine, Baldauf, Sandra L., Schaap, Pauline
Phenotypic novelties can arise if integrated developmental pathways are expressed at new developmental stages and then recruited to serve new functions. We analyze the origin of a novel developmental...
Functional Promiscuity of Gene Regulation by Serpentine Receptors in Dictyostelium discoideum
Kim, Ji-Yun, Brandt, Raymond, Devreotes, Peter N., Schaap, Pauline
Serpentine receptors such as smoothened and frizzled play important roles in cell fate determination during animal development. In Dictyostelium discoideum, four serpentine cyclic AMP (cAMP)...
The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase
Roelofs, Jeroen, Meima, Marcel, Schaap, Pauline
A new Dictyostelium discoideum cyclase gene was identified that encodes a protein (sGC) with 35% similarity to mammalian soluble adenylyl cyclase (sAC). Gene disruption of sGC has no effect on...
Meima, Marcel E., Biondi, Ricardo M., Schaap, Pauline
StmF mutants are chemotactic mutants that are defective in a cGMP phosphodiesterase (PDE) activity. We identified a novel gene, PdeD, that harbors two cyclic nucleotide–binding domains and a...
Schaap, Pauline, Konijn, Theo M.
Aggregation in the primitive cellular slime mold Dictyostelium minutum proceeds by means of chemotaxis toward a continuously secreted folic acid analog [De Wit, R. J. W. & Konijn, T. M. (1983) Cell...
Adenylyl Cyclase G Is Activated by an Intramolecular Osmosensor
Saran, Shweta, Schaap, Pauline
Adenylyl cyclase G (ACG) is activated by high osmolality and mediates inhibition of spore germination by this stress factor. The catalytic domains of all eukaryote cyclases are active as dimers and...
Evolutionary origin of cAMP-based chemoattraction in the social amoebae
Alvarez-Curto, Elisa, Rozen, Daniel E., Ritchie, Allyson V., Fouquet, Celine, Baldauf, Sandra L., Schaap, Pauline
Phenotypic novelties can arise if integrated developmental pathways are expressed at new developmental stages and then recruited to serve new functions. We analyze the origin of a novel developmental...
Meima, Marcel E., Weening, Karin E., Schaap, Pauline
We constructed a series of expression vectors for purification of native proteins and protein complexes in Dictyostelium. Protein purification is achieved by either a C-terminal or N-terminal fusion...
Pharmacological profiling of the Dictyostelium adenylate cyclases ACA, ACB and ACG
Alvarez-Curto, Elisa, Weening, Karin E., Schaap, Pauline
Intracellular and secreted cAMPs play crucial roles in controlling cell movement and gene regulation throughout development of the social amoeba Dictyostelium discoideum. cAMP is produced by three...
From Drought Sensing to Developmental Control: Evolution of Cyclic AMP Signaling in Social Amoebas
Ritchie, Allyson V., Van Es, Saskia, Fouquet, Celine, Schaap, Pauline
Amoebas and other protists commonly encyst when faced with environmental stress. Although little is known of the signaling pathways that mediate encystation, the analogous process of spore formation...
Activated cAMP receptors switch encystation into sporulation
Kawabe, Yoshinori, Morio, Takahiro, James, John L., Prescott, Alan R., Tanaka, Yoshimasa, Schaap, Pauline
Metazoan embryogenesis is controlled by a limited number of signaling modules that are used repetitively at successive developmental stages. The development of social amoebas shows similar reiterated...