Origin and function of the two major tail proteins of bacteriophage SPP1 (2008)
Auzat, Isabelle, Dröge, Anja, Weise, Frank, Lurz, Rudi, Tavares, Paulo
The majority of bacteriophages have a long non-contractile tail (Siphoviridae) that serves as a conduit for viral DNA traffic from the phage capsid to the host cell at the beginning of infection. The...
Oligomerization of the SPP1 scaffolding protein (2008)
Poh, Siew Lay, El Khadali, Fatima, Berrier, Catherine, Lurz, Rudi, Melki, Ronald, Tavares, Paulo
Viral scaffolding proteins direct polymerization of major capsid protein subunits into icosahedral procapsid structures. The scaffolding protein of bacteriophage SPP1 was engineered with a C-terminal...
Resende,Jefferson G., Menezes,Cristiane G., Paula,Ana M. C., Ferreira,Antonio C. P., Zaconeta,Carlos A. M., Silva,César A. M., ...
OBJETIVO: Avaliar o pico de pressão inspiratória e a freqüência ventilatória obtidos por médicos quando da utilização de balão auto-inflável neonatal em modelo de pulmão. MÉTODOS: Quinze...
Resende,Jefferson G., Zaconeta,Carlos A. M., Ferreira,Antonio C. P., Silva,César A. M., Rodrigues,Marcelo P., Rebello,Celso M., ...
OBJETIVO: Avaliar o pico de pressão inspiratória, o volume corrente e a freqüência respiratória obtidos durante ventilação manual de carneiros prematuros, utilizando balão auto-inflável....
Análise da mecânica pulmonar em modelo experimental de sepse (2006)
Carvalho,Rodrigo Storck, Gaio,Carlos Eduardo, Tavares,Paulo
OBJETIVO: Verificar se há alterações na mecânica pulmonar de camundongos com sepse. MÉTODOS: Foram utilizados 40 camundongos Balb/c divididos em dois grupos: sobrevida (n = 21) e mecânica...
Análise da mecânica pulmonar em modelo experimental de sepse (2006)
Carvalho,Rodrigo Storck, Gaio,Carlos Eduardo, Tavares,Paulo
OBJETIVO: Verificar se há alterações na mecânica pulmonar de camundongos com sepse. MÉTODOS: Foram utilizados 40 camundongos Balb/c divididos em dois grupos: sobrevida (n = 21) e mecânica...
Stiege,Asita C., Isidro,Anabela, Droege,Anja, Tavares,Paulo
An essential component in the assembly of nucleocapsids of tailed bacteriophages and of herpes viruses is the portal protein that is located at the unique vertex of the icosahedral capsid through...
Camacho,Ana G., Gual,Aranzazu, Lurz,Rudi, Tavares,Paulo, Alonso,Juan C.
Initiation of headful packaging of SPP1 DNA concatemers involves the interaction of the terminase, G1P and G2P, and the portal protein, G6P. G1P, which specifically recognizes the non-adjacent pacL...
Camacho,Ana G., Gual,Aranzazu, Lurz,Rudi, Tavares,Paulo, Alonso,Juan C.
Initiation of headful packaging of SPP1 DNA concatemers involves the interaction of the terminase, G1P and G2P, and the portal protein, G6P. G1P, which specifically recognizes the non-adjacent pacL...
Structure of a viral DNA gatekeeper at 10 angstrom resolution by cryo-electron microscopy (2003)
Orlova,Elena V., Gowen,Brent, Dröge,Anja, Stiege,Asita, Weise,Frank, Lurz,Rudi, ...
In tailed bacteriophages and herpes viruses, the viral DNA is packaged through the portal protein channel. Channel closure is essential to prevent DNA release after packaging. Here we present the...
Camacho, Ana G., Gual, Aranzazu, Lurz, Rudi, Tavares, Paulo, Alonso, Juan C.
Initiation of headful packaging of SPP1 DNA concatemers involves the interaction of the terminase, G1P and G2P, and the portal protein, G6P. G1P, which specifically recognizes the non-adjacent pacL...
Camacho, Ana G., Gual, Aranzazu, Lurz, Rudi, Tavares, Paulo, Alonso, Juan C.
Initiation of headful packaging of SPP1 DNA concatemers involves the interaction of the terminase, G1P and G2P, and the portal protein, G6P. G1P, which specifically recognizes the non-adjacent pacL...
Structure of a viral DNA gatekeeper at 10 angstrom resolution by cryo-electron microscopy (2003)
Orlova, Elena V., Gowen, Brent, Dröge, Anja, Stiege, Asita, Weise, Frank, Lurz, Rudi, ...
In tailed bacteriophages and herpes viruses, the viral DNA is packaged through the portal protein channel. Channel closure is essential to prevent DNA release after packaging. Here we present the...
Stiege, Asita C., Isidro, Anabela, Droege, Anja, Tavares, Paulo
An essential component in the assembly of nucleocapsids of tailed bacteriophages and of herpes viruses is the portal protein that is located at the unique vertex of the icosahedral capsid through...
Daytime sleepiness and academic performance in medical students (2002)
Rodrigues,Raimundo Nonato D., Viegas,Carlos A.A., Tavares,Paulo
This report presents an analysis of the complaints of increasing daytime sleepiness as well as a study on their possible effects on the academic performance of medical students at the University of...
Jekow,Petra, Behlke,Joachim, Tichelaar,Willem, Lurz,Rudi, Regalla,Manuela, Hinrichs,Winfried, ...
Bacteriophage SPP1 portal protein is a large cyclical homo-oligomer composed of 13 subunits. The solution structure and assembly behavior of this protein with high-point rotational symmetry was...
Jekow, Petra, Behlke, Joachim, Tichelaar, Willem, Lurz, Rudi, Regalla, Manuela, Hinrichs, Winfried, ...
Bacteriophage SPP1 portal protein is a large cyclical homo-oligomer composed of 13 subunits. The solution structure and assembly behavior of this protein with high-point rotational symmetry was...
Rigothier, Marie-Christine, Khun, Hout, Tavares, Paulo, Cardona, Ana, Huerre, Michel, Guillén, Nancy
The protozoan parasite Entamoeba histolytica is the causative agent of amoebiasis, a human disease characterized by dysentery and liver abscess. The physiopathology of hepatic lesions can be...
Structure of a viral DNA gatekeeper at 10 Å resolution by cryo-electron microscopy
Orlova, Elena V., Gowen, Brent, Dröge, Anja, Stiege, Asita, Weise, Frank, Lurz, Rudi, ...
In tailed bacteriophages and herpes viruses, the viral DNA is packaged through the portal protein channel. Channel closure is essential to prevent DNA release after packaging. Here we present the...
Tavares, Paulo, Rigothier, Marie-Christine, Khun, Huot, Roux, Pascal, Huerre, Michel, Guillén, Nancy
The protozoan parasite Entamoeba histolytica colonizes the human large bowel. Invasion of the intestinal epithelium causes amoebic colitis and opens the route for amoebic liver abscesses. The...
Rigothier, Marie-Christine, Khun, Hout, Tavares, Paulo, Cardona, Ana, Huerre, Michel, Guillén, Nancy
The protozoan parasite Entamoeba histolytica is the causative agent of amoebiasis, a human disease characterized by dysentery and liver abscess. The physiopathology of hepatic lesions can be...
Structure of a viral DNA gatekeeper at 10 Å resolution by cryo-electron microscopy
Orlova, Elena V., Gowen, Brent, Dröge, Anja, Stiege, Asita, Weise, Frank, Lurz, Rudi, ...
In tailed bacteriophages and herpes viruses, the viral DNA is packaged through the portal protein channel. Channel closure is essential to prevent DNA release after packaging. Here we present the...
Tavares, Paulo, Rigothier, Marie-Christine, Khun, Huot, Roux, Pascal, Huerre, Michel, Guillén, Nancy
The protozoan parasite Entamoeba histolytica colonizes the human large bowel. Invasion of the intestinal epithelium causes amoebic colitis and opens the route for amoebic liver abscesses. The...
Structural framework for DNA translocation via the viral portal protein
Lebedev, Andrey A, Krause, Margret H, Isidro, Anabela L, Vagin, Alexei A, Orlova, Elena V, Turner, Joanne, ...
Tailed bacteriophages and herpesviruses load their capsids with DNA through a tunnel formed by the portal protein assembly. Here we describe the X-ray structure of the bacteriophage SPP1 portal...
Structure of bacteriophage SPP1 tail reveals trigger for DNA ejection
Plisson, Celia, White, Helen E, Auzat, Isabelle, Zafarani, Amineh, São-José, Carlos, Lhuillier, Sophie, ...
The majority of known bacteriophages have long noncontractile tails (Siphoviridae) that serve as a pipeline for genome delivery into the host cytoplasm. The tail extremity distal from the phage head...
A Kinetic Analysis of DNA Ejection from Tailed Phages Revealing the Prerequisite Activation Energy
Raspaud, Eric, Forth, Thomas, São-José, Carlos, Tavares, Paulo, De Frutos, Marta
All tailed bacteriophages follow the same general scheme of infection: they bind to their specific host receptor and then transfer their genome into the bacterium. DNA translocation is thought to be...