Gómez-Rodríguez, Julio, Washington, Valance, Cheng, Jun, Dutra, Amalia, Pak, Evgenia, Liu, Pentao, ...
We evaluate here the use of real-time quantitative PCR (q-PCR) as a method for screening for homologous recombinants generated in mammalian cells from either conventional gene-targeting constructs or...
Chan, Waiin, Costantino, Nina, Li, Ruixue, Lee, Song Choon, Su, Qin, Melvin, David, ...
Functional analysis of mammalian genes in vivo is primarily achieved through analysing knockout mice. Now that the sequencing of several mammalian genomes has been completed, understanding functions...
David J. Adams, Michael A. Quail, Tony Cox, Barbara D. Gorick, Qin Su, ...
The majority of gene-targeting experiments in mice are performed in 129Sv-derived embryonic stem (ES) cell lines, which are generally considered to be more reliable at colonizing the germ line than...
A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout Mutations (2003)
Liu, Pentao, Jenkins, Nancy A., Copeland, Neal G.
Phage-based Escherichia coli homologous recombination systems have recently been developed that now make it possible to subclone or modify DNA cloned into plasmids, BACs, or PACs without the need for...
Chromosome engineering in mice / (1998)
Thesis (Ph. D.)--Baylor College of Medicine. Developmental Biology Program, 1998.
A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout Mutations
Liu, Pentao, Jenkins, Nancy A., Copeland, Neal G.
Phage-based Escherichia coli homologous recombination systems have recently been developed that now make it possible to subclone or modify DNA cloned into plasmids, BACs, or PACs without the need for...
Chen, You-Tzung, Liu, Pentao, Bradley, Allan
Gene trapping in mouse embryonic stem cells is an important genetic approach that allows simultaneous mutation of genes and generation of corresponding mutant mice. We designed a selection scheme...
A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout Mutations
Liu, Pentao, Jenkins, Nancy A., Copeland, Neal G.
Phage-based Escherichia coli homologous recombination systems have recently been developed that now make it possible to subclone or modify DNA cloned into plasmids, BACs, or PACs without the need for...
Chen, You-Tzung, Liu, Pentao, Bradley, Allan
Gene trapping in mouse embryonic stem cells is an important genetic approach that allows simultaneous mutation of genes and generation of corresponding mutant mice. We designed a selection scheme...
Chan, Waiin, Costantino, Nina, Li, Ruixue, Lee, Song Choon, Su, Qin, Melvin, David, ...
Functional analysis of mammalian genes in vivo is primarily achieved through analysing knockout mice. Now that the sequencing of several mammalian genomes has been completed, understanding functions...
BCL11B is required for positive selection and survival of double-positive thymocytes
Albu, Diana I., Feng, Dongyun, Bhattacharya, Debarati, Jenkins, Nancy A., Copeland, Neal G., Liu, Pentao, ...
Transcriptional control of gene expression in double-positive (DP) thymocytes remains poorly understood. We show that the transcription factor BCL11B plays a critical role in DP thymocytes by...
Gómez-Rodríguez, Julio, Washington, Valance, Cheng, Jun, Dutra, Amalia, Pak, Evgenia, Liu, Pentao, ...
We evaluate here the use of real-time quantitative PCR (q-PCR) as a method for screening for homologous recombinants generated in mammalian cells from either conventional gene-targeting constructs or...
Chromosomal transposition of PiggyBac in mouse embryonic stem cells
Wang, Wei, Lin, Chengyi, Lu, Dong, Ning, Zeming, Cox, Tony, Melvin, David, ...
Transposon systems are widely used for generating mutations in various model organisms. PiggyBac (PB) has recently been shown to transpose efficiently in the mouse germ line and other mammalian cell...
A DNA transposon-based approach to validate oncogenic mutations in the mouse
Su, Qin, Prosser, Haydn M., Campos, Lia S., Ortiz, Mariaestela, Nakamura, Takuro, Warren, Madhuri, ...
Large-scale cancer genome projects will soon be able to sequence many cancer genomes to comprehensively identify genetic changes in human cancer. Genome-wide association studies have also identified...