Bellomo, Elisa A, Wijesekara, Nadeeja, Loder, Merewyn K, Baldwin, Jocelyn M, Gyulkhandanyan, Armen V, ...
Objective. Zinc ions are essential for the formation of hexameric insulin and hormone crystallisation. Correspondingly, a non-synonymous single nucleotide polymorphism rs13266634 in the SLC30A8 gene,...
Advisor: Peter Arvan.
Kuliawat, Regina, Prabakaran, Daniel, Arvan, Peter
Recently, two different prohormone-processing enzymes, prohormone convertase 1 (PC1) and carboxypeptidase E, have been implicated in enhancing the storage of peptide hormones in endocrine secretory...
Kim, Paul S., Hossain, Shaikh Abu, Park, Young-Nam, Lee, Ike, Yoo, Sung-Eun, Arvan, Peter
Newly synthesized thyroglobulin (Tg), the major secretory glycoprotein of the thyroid gland, folds and homodimerizes in the endoplasmic reticulum (ER) before its export to the site of iodination,...
Syntaxin-6 SNARE Involvement in Secretory and Endocytic Pathways of Cultured Pancreatic β-Cells
Kuliawat, Regina, Kalinina, Elena, Bock, Jason, Fricker, Lloyd, McGraw, Timothy E., Kim, Se Ryoung, ...
In pancreatic β-cells, the syntaxin 6 (Syn6) soluble N-ethylmaleimide-sensitive factor attachment protein receptor is distributed in the trans-Golgi network (TGN) (with spillover into immature...
Dunham, Philip, Arvan, Peter, Falkow, Stephen, Hoffstein, Sylvia, Weissmann, Gerald
Blood phagocytes of the dogfish Mustelus canis attack oocytes of the sea urchin Arbacia punctulata, first provoking a surrogate fertilization response and then killing the eggs. To test the...
Di Jeso, Bruno, Ulianich, Luca, Pacifico, Francesco, Leonardi, Antonio, Vito, Pasquale, Consiglio, Eduardo, ...
During its initial folding in the endoplasmic reticulum (ER), newly synthesized thyroglobulin (Tg) is known to interact with calnexin and other ER molecular chaperones, but its interaction with...
Di Jeso, Bruno, Park, Young-nam, Ulianich, Luca, Treglia, A. Sonia, Urbanas, Malene L., High, Stephen, ...
We present the first identification of transient folding intermediates of endogenous thyroglobulin (Tg; a large homodimeric secretory glycoprotein of thyrocytes), which include mixed disulfides with...
Kuliawat, Regina, Prabakaran, Daniel, Arvan, Peter
Recently, two different prohormone-processing enzymes, prohormone convertase 1 (PC1) and carboxypeptidase E, have been implicated in enhancing the storage of peptide hormones in endocrine secretory...
Kim, Paul S., Hossain, Shaikh Abu, Park, Young-Nam, Lee, Ike, Yoo, Sung-Eun, Arvan, Peter
Newly synthesized thyroglobulin (Tg), the major secretory glycoprotein of the thyroid gland, folds and homodimerizes in the endoplasmic reticulum (ER) before its export to the site of iodination,...
Syntaxin-6 SNARE Involvement in Secretory and Endocytic Pathways of Cultured Pancreatic β-Cells
Kuliawat, Regina, Kalinina, Elena, Bock, Jason, Fricker, Lloyd, McGraw, Timothy E., Kim, Se Ryoung, ...
In pancreatic β-cells, the syntaxin 6 (Syn6) soluble N-ethylmaleimide-sensitive factor attachment protein receptor is distributed in the trans-Golgi network (TGN) (with spillover into immature...
Dunham, Philip, Arvan, Peter, Falkow, Stephen, Hoffstein, Sylvia, Weissmann, Gerald
Blood phagocytes of the dogfish Mustelus canis attack oocytes of the sea urchin Arbacia punctulata, first provoking a surrogate fertilization response and then killing the eggs. To test the...
Di Jeso, Bruno, Ulianich, Luca, Pacifico, Francesco, Leonardi, Antonio, Vito, Pasquale, Consiglio, Eduardo, ...
During its initial folding in the endoplasmic reticulum (ER), newly synthesized thyroglobulin (Tg) is known to interact with calnexin and other ER molecular chaperones, but its interaction with...
Di Jeso, Bruno, Park, Young-nam, Ulianich, Luca, Treglia, A. Sonia, Urbanas, Malene L., High, Stephen, ...
We present the first identification of transient folding intermediates of endogenous thyroglobulin (Tg; a large homodimeric secretory glycoprotein of thyrocytes), which include mixed disulfides with...
Protein disulfide isomerase–like proteins play opposing roles during retrotranslocation
Forster, Michele L., Sivick, Kelsey, Park, Young-nam, Arvan, Peter, Lencer, Wayne I., Tsai, Billy
Misfolded proteins in the endoplasmic reticulum (ER) are retained in the organelle or retrotranslocated to the cytosol for proteasomal degradation. ER chaperones that guide these opposing processes...
Proinsulin maturation, misfolding, and proteotoxicity
Liu, Ming, Hodish, Israel, Rhodes, Christopher J., Arvan, Peter
As a tool to explore proinsulin (PI) trafficking, a human PI cDNA has been constructed with GFP fused within the C peptide. In regulated secretory cells containing appropriate prohormone convertases,...
Kuliawat, Regina, Klumperman, Judith, Ludwig, Thomas, Arvan, Peter
In cells specialized for secretory granule exocytosis, lysosomal hydrolases may enter the regulated secretory pathway. Using mouse pancreatic islets and the INS-1 β-cell line as models, we have...
Klumperman, Judith, Kuliawat, Regina, Griffith, Janice M., Geuze, Hans J., Arvan, Peter
The occurrence of clathrin-coated buds on immature granules (IGs) of the regulated secretory pathway suggests that specific transmembrane proteins are sorted into these buds through interaction with...
Zhang, Bao-yan, Chang, Amy, Kjeldsen, Thomas B., Arvan, Peter
An insulin-containing fusion protein (ICFP, encoding the yeast prepro-α factor leader peptide fused via a lysine-arginine cleavage site to a single chain insulin) has been expressed in Saccharomyces...
Colombo, Carlo, Porzio, Ottavia, Liu, Ming, Massa, Ornella, Vasta, Mario, Salardi, Silvana, ...
Permanent neonatal diabetes mellitus (PNDM) is a rare disorder usually presenting within 6 months of birth. Although several genes have been linked to this disorder, in almost half the cases...
The cholinesterase-like domain of thyroglobulin functions as an intramolecular chaperone
Lee, Jaemin, Di Jeso, Bruno, Arvan, Peter
Thyroid hormonogenesis requires secretion of thyroglobulin, a protein comprising Cys-rich regions I, II, and III (referred to collectively as region I-II-III) followed by a cholinesterase-like (ChEL)...
Yamaguchi, Yukihiro, Larkin, Dennis, Lara-Lemus, Roberto, Ramos-Castañeda, Jose, Liu, Ming, Arvan, Peter
The activity of PERK, an endoplasmic reticulum (ER) transmembrane protein kinase, assists in an ER stress response designed to inhibit general protein synthesis while allowing upregulated synthesis...
Kim, Paul S., Lee, Jaemin, Jongsamak, Piyanuch, Menon, Shekar, Li, Bailing, Hossain, Shaikh A., ...
It has been suggested that a thyroglobulin (Tg)-R19K missense mutation may be a newly identified cause of human congenital goiter, which is surprising for this seemingly conservative substitution....
Targeted Induction of Endoplasmic Reticulum Stress Induces Cartilage Pathology
Rajpar, M. Helen, McDermott, Ben, Kung, Louise, Eardley, Rachel, Knowles, Lynette, Heeran, Mel, ...
Pathologies caused by mutations in extracellular matrix proteins are generally considered to result from the synthesis of extracellular matrices that are defective. Mutations in type X collagen cause...