Peter E. Reynolds

The vanC-3 vancomycin resistance gene cluster of Enterococcus flavescens CCM 439 (2003)

Dutta, Ireena, Reynolds, Peter E.

Enterococcus flavescens CCM 439 is phenotypically similar to Enterococcus casseliflavus; it possesses intrinsic low-level resistance to vancomycin and has the VanC phenotype. The complete vanC-3...

The vanC-3 vancomycin resistance gene cluster of Enterococcus flavescens CCM 439 (2003)

Dutta, Ireena, Reynolds, Peter E.

Enterococcus flavescens CCM 439 is phenotypically similar to Enterococcus casseliflavus; it possesses intrinsic low-level resistance to vancomycin and has the VanC phenotype. The complete vanC-3...

The vanC-3 vancomycin resistance gene cluster of Enterococcus flavescens CCM 439 (2003)

Dutta, Ireena, Reynolds, Peter E.

Enterococcus flavescens CCM 439 is phenotypically similar to Enterococcus casseliflavus; it possesses intrinsic low-level resistance to vancomycin and has the VanC phenotype. The complete vanC-3...

Vancomycin-Dependent Enterococcus faecalis Clinical Isolates and Revertant Mutants

Van Bambeke, Françoise, Chauvel, Murielle, Reynolds, Peter E., Fraimow, Henry S., Courvalin, Patrice

Three vancomycin-dependent clinical isolates of Enterococcus faecalis of the VanB type were studied by determining (i) the sequence of the ddl gene encoding the host d-Ala:d-Ala ligase and the...

vanC Cluster of Vancomycin-Resistant Enterococcus gallinarum BM4174

Arias, Cesar A., Courvalin, Patrice, Reynolds, Peter E.

Glycopeptide-resistant enterococci of the VanC type synthesize UDP-muramyl-pentapeptide[d-Ser] for cell wall assembly and prevent synthesis of peptidoglycan precursors ending in d-Ala. The vanC...

Characterization of a Divergent vanD-Type Resistance Element from the First Glycopeptide-Resistant Strain of Enterococcus faecium Isolated in Brazil

Dalla Costa, Libera M., Reynolds, Peter E., Souza, Helena A. P. H. M., Souza, Dilair C., Palepou, Marie-France I., Woodford, Neil

Enterococcus faecium 10/96A from Brazil was resistant to vancomycin (MIC, 256 μg/ml) but gave no amplification products with primers specific for known van genotypes. A 2,368-bp fragment of a van...

Regulation of Expression of the vanD Glycopeptide Resistance Gene Cluster from Enterococcus faecium BM4339

Casadewall, Barbara, Reynolds, Peter E., Courvalin, Patrice

A new open reading frame, encoding a putative integrase-like protein, was detected downstream from the six genes of the vanD glycopeptide resistance cluster in Enterococcus faecium BM4339 (B....

The Lantibiotic Mersacidin Inhibits Peptidoglycan Synthesis by Targeting Lipid II

Brötz, Heike, Bierbaum, Gabriele, Leopold, Klaus, Reynolds, Peter E., Sahl, Hans-Georg

The lantibiotic mersacidin exerts its bactericidal action by inhibition of peptidoglycan biosynthesis. It interferes with the membrane-associated transglycosylation reaction; during this step the...

d-Ala:d-Ala Ligase Gene Flanking the vanC Cluster: Evidence for Presence of Three Ligase Genes in Vancomycin-Resistant Enterococcus gallinarum BM4174

Ambúr, Ole-Herman, Reynolds, Peter E., Arias, Cesar A.

An open reading frame located 230 nucleotides downstream from the stop codon of vanSc and in the opposite direction relative to the other genes of the vanC cluster was identified in Enterococcus...

Biochemical and Genetic Characterization of the vanC-2 Vancomycin Resistance Gene Cluster of Enterococcus casseliflavus ATCC 25788

Dutta, Ireena, Reynolds, Peter E.

The vanC-2 cluster of Enterococcus casseliflavus ATCC 25788 consisted of five genes (vanC-2, vanXYC-2, vanTC-2, vanRC-2, and vanSC-2) and shared the same organization as the vanC cluster of E....

VanD-Type Vancomycin-Resistant Enterococcus faecium 10/96A

Depardieu, Florence, Reynolds, Peter E., Courvalin, Patrice

VanD type Enterococcus faecium 10/96A is constitutively resistant to vancomycin and to low levels of teicoplanin by nearly exclusive synthesis of peptidoglycan precursors terminating in...

Transcriptional Analysis of the vanC Cluster from Enterococcus gallinarum Strains with Constitutive and Inducible Vancomycin Resistance

Panesso, Diana, Abadía-Patiño, Lorena, Vanegas, Natasha, Reynolds, Peter E., Courvalin, Patrice, Arias, Cesar A.

The vanC glycopeptide resistance gene cluster encodes enzymes required for synthesis of peptidoglycan precursors ending in d-Ala-d-Ser. Enterococcus gallinarum BM4174 and SC1 are constitutively and...

Binding of β-lactam antibiotics to the exocellular dd-carboxypeptidase–transpeptidase of Streptomyces R39

Frère, Jean-Marie, Ghuysen, Jean-Marie, Reynolds, Peter E., Moreno, Ramon, Perkins, Harold R.

Benzylpenicillin and cephaloridine reacted with the exocellular dd-carboxypeptidase–transpeptidase from Streptomyces R39 to form equimolar and inactive antibiotic–enzyme complexes. At saturation,...

Vancomycin-Dependent Enterococcus faecalis Clinical Isolates and Revertant Mutants

Van Bambeke, Françoise, Chauvel, Murielle, Reynolds, Peter E., Fraimow, Henry S., Courvalin, Patrice

Three vancomycin-dependent clinical isolates of Enterococcus faecalis of the VanB type were studied by determining (i) the sequence of the ddl gene encoding the host d-Ala:d-Ala ligase and the...

vanC Cluster of Vancomycin-Resistant Enterococcus gallinarum BM4174

Arias, Cesar A., Courvalin, Patrice, Reynolds, Peter E.

Glycopeptide-resistant enterococci of the VanC type synthesize UDP-muramyl-pentapeptide[d-Ser] for cell wall assembly and prevent synthesis of peptidoglycan precursors ending in d-Ala. The vanC...

Characterization of a Divergent vanD-Type Resistance Element from the First Glycopeptide-Resistant Strain of Enterococcus faecium Isolated in Brazil

Dalla Costa, Libera M., Reynolds, Peter E., Souza, Helena A. P. H. M., Souza, Dilair C., Palepou, Marie-France I., Woodford, Neil

Enterococcus faecium 10/96A from Brazil was resistant to vancomycin (MIC, 256 μg/ml) but gave no amplification products with primers specific for known van genotypes. A 2,368-bp fragment of a van...

Regulation of Expression of the vanD Glycopeptide Resistance Gene Cluster from Enterococcus faecium BM4339

Casadewall, Barbara, Reynolds, Peter E., Courvalin, Patrice

A new open reading frame, encoding a putative integrase-like protein, was detected downstream from the six genes of the vanD glycopeptide resistance cluster in Enterococcus faecium BM4339 (B....

The Lantibiotic Mersacidin Inhibits Peptidoglycan Synthesis by Targeting Lipid II

Brötz, Heike, Bierbaum, Gabriele, Leopold, Klaus, Reynolds, Peter E., Sahl, Hans-Georg

The lantibiotic mersacidin exerts its bactericidal action by inhibition of peptidoglycan biosynthesis. It interferes with the membrane-associated transglycosylation reaction; during this step the...

d-Ala:d-Ala Ligase Gene Flanking the vanC Cluster: Evidence for Presence of Three Ligase Genes in Vancomycin-Resistant Enterococcus gallinarum BM4174

Ambúr, Ole-Herman, Reynolds, Peter E., Arias, Cesar A.

An open reading frame located 230 nucleotides downstream from the stop codon of vanSc and in the opposite direction relative to the other genes of the vanC cluster was identified in Enterococcus...

Biochemical and Genetic Characterization of the vanC-2 Vancomycin Resistance Gene Cluster of Enterococcus casseliflavus ATCC 25788

Dutta, Ireena, Reynolds, Peter E.

The vanC-2 cluster of Enterococcus casseliflavus ATCC 25788 consisted of five genes (vanC-2, vanXYC-2, vanTC-2, vanRC-2, and vanSC-2) and shared the same organization as the vanC cluster of E....

VanD-Type Vancomycin-Resistant Enterococcus faecium 10/96A

Depardieu, Florence, Reynolds, Peter E., Courvalin, Patrice

VanD type Enterococcus faecium 10/96A is constitutively resistant to vancomycin and to low levels of teicoplanin by nearly exclusive synthesis of peptidoglycan precursors terminating in...

Transcriptional Analysis of the vanC Cluster from Enterococcus gallinarum Strains with Constitutive and Inducible Vancomycin Resistance

Panesso, Diana, Abadía-Patiño, Lorena, Vanegas, Natasha, Reynolds, Peter E., Courvalin, Patrice, Arias, Cesar A.

The vanC glycopeptide resistance gene cluster encodes enzymes required for synthesis of peptidoglycan precursors ending in d-Ala-d-Ser. Enterococcus gallinarum BM4174 and SC1 are constitutively and...

Binding of β-lactam antibiotics to the exocellular dd-carboxypeptidase–transpeptidase of Streptomyces R39

Frère, Jean-Marie, Ghuysen, Jean-Marie, Reynolds, Peter E., Moreno, Ramon, Perkins, Harold R.

Benzylpenicillin and cephaloridine reacted with the exocellular dd-carboxypeptidase–transpeptidase from Streptomyces R39 to form equimolar and inactive antibiotic–enzyme complexes. At saturation,...