Zhou, Xue-Rong, Christie, Peter J.
The VirE2 single-stranded DNA-binding protein (SSB) of Agrobacterium tumefaciens is required for delivery of T-DNA to the nuclei of susceptible plant cells. By yeast two-hybrid and...
VirB7 Lipoprotein Is Exocellular and Associates with the Agrobacterium tumefaciens T Pilus
Sagulenko, Vitaliya, Sagulenko, Evgeniy, Jakubowski, Simon, Spudich, Elena, Christie, Peter J.
Agrobacterium tumefaciens transfers oncogenic T-DNA and effector proteins to plant cells via a type IV secretion pathway. This transfer system, assembled from the products of the virB operon, is...
Zhao, Zhenming, Sagulenko, Evgeniy, Ding, Zhiyong, Christie, Peter J.
Agrobacterium tumefaciens uses a type IV secretion system to deliver oncogenic nucleoprotein particles and effector proteins, such as the multifunctional VirE2 protein, to plant cells. In this study,...
Role of Agrobacterium VirB11 ATPase in T-Pilus Assembly and Substrate Selection
Sagulenko, Evgeniy, Sagulenko, Vitaliya, Chen, Jun, Christie, Peter J.
The VirB11 ATPase is a subunit of the Agrobacterium tumefaciens transfer DNA (T-DNA) transfer system, a type IV secretion pathway required for delivery of T-DNA and effector proteins to plant cells...
Self-Assembly of the Agrobacterium tumefaciens VirB11 Traffic ATPase
Rashkova, Svetlana, Zhou, Xue-Rong, Chen, Jun, Christie, Peter J.
The Agrobacterium tumefaciens VirB11 ATPase is a component of a type IV transporter dedicated to T-DNA delivery to plant cells. In this study, we tested a prediction from genetic findings that VirB11...
Ding, Zhiyong, Zhao, Zhenming, Jakubowski, Simon J., Krishnamohan, Atmakuri, Margolin, William, Christie, Peter J.
DivIVA of Bacillus subtilis and FtsZ of Escherichia coli were used to target heterologous protein complexes to cell division sites of E. coli and Agrobacterium tumefaciens. DivIVA and FtsZ that were...
Ding, Zhiyong, Christie, Peter J.
This study characterized the contribution of the twin-arginine translocation (TAT) pathway to growth, motility, and virulence of the phytopathogen Agrobacterium tumefaciens. In contrast to wild-type...
Jakubowski, Simon J., Krishnamoorthy, Vidhya, Christie, Peter J.
This study characterized the contribution of Agrobacterium tumefaciens VirB6, a polytopic inner membrane protein, to the formation of outer membrane VirB7 lipoprotein and VirB9 protein multimers...
Agrobacterium VirB10, an ATP energy sensor required for type IV secretion
Cascales, Eric, Christie, Peter J.
Bacteria use type IV secretion systems (T4SS) to translocate DNA and protein substrates to target cells of phylogenetically diverse taxa. Recently, by use of an assay termed transfer DNA...
Christie, Peter J., Hahn, Matthias, Walbot, Virginia
Low-temperature stress was shown to cause a rapid increase in steady-state levels of alcohol dehydrogenase-1 message (Adh1) and protein activity (ADH1) in maize (Zea mays) (B37N, A188) and rice...
Jakubowski, Simon J., Cascales, Eric, Krishnamoorthy, Vidhya, Christie, Peter J.
Agrobacterium tumefaciens translocates DNA and protein substrates between cells via a type IV secretion system (T4SS) whose channel subunits include the VirD4 coupling protein, VirB11 ATPase, VirB6,...
Zhou, Xue-Rong, Christie, Peter J.
The VirE2 single-stranded DNA-binding protein (SSB) of Agrobacterium tumefaciens is required for delivery of T-DNA to the nuclei of susceptible plant cells. By yeast two-hybrid and...
VirB7 Lipoprotein Is Exocellular and Associates with the Agrobacterium tumefaciens T Pilus
Sagulenko, Vitaliya, Sagulenko, Evgeniy, Jakubowski, Simon, Spudich, Elena, Christie, Peter J.
Agrobacterium tumefaciens transfers oncogenic T-DNA and effector proteins to plant cells via a type IV secretion pathway. This transfer system, assembled from the products of the virB operon, is...
Zhao, Zhenming, Sagulenko, Evgeniy, Ding, Zhiyong, Christie, Peter J.
Agrobacterium tumefaciens uses a type IV secretion system to deliver oncogenic nucleoprotein particles and effector proteins, such as the multifunctional VirE2 protein, to plant cells. In this study,...
Role of Agrobacterium VirB11 ATPase in T-Pilus Assembly and Substrate Selection
Sagulenko, Evgeniy, Sagulenko, Vitaliya, Chen, Jun, Christie, Peter J.
The VirB11 ATPase is a subunit of the Agrobacterium tumefaciens transfer DNA (T-DNA) transfer system, a type IV secretion pathway required for delivery of T-DNA and effector proteins to plant cells...
Self-Assembly of the Agrobacterium tumefaciens VirB11 Traffic ATPase
Rashkova, Svetlana, Zhou, Xue-Rong, Chen, Jun, Christie, Peter J.
The Agrobacterium tumefaciens VirB11 ATPase is a component of a type IV transporter dedicated to T-DNA delivery to plant cells. In this study, we tested a prediction from genetic findings that VirB11...
Ding, Zhiyong, Zhao, Zhenming, Jakubowski, Simon J., Krishnamohan, Atmakuri, Margolin, William, Christie, Peter J.
DivIVA of Bacillus subtilis and FtsZ of Escherichia coli were used to target heterologous protein complexes to cell division sites of E. coli and Agrobacterium tumefaciens. DivIVA and FtsZ that were...
Ding, Zhiyong, Christie, Peter J.
This study characterized the contribution of the twin-arginine translocation (TAT) pathway to growth, motility, and virulence of the phytopathogen Agrobacterium tumefaciens. In contrast to wild-type...
Jakubowski, Simon J., Krishnamoorthy, Vidhya, Christie, Peter J.
This study characterized the contribution of Agrobacterium tumefaciens VirB6, a polytopic inner membrane protein, to the formation of outer membrane VirB7 lipoprotein and VirB9 protein multimers...
Agrobacterium VirB10, an ATP energy sensor required for type IV secretion
Cascales, Eric, Christie, Peter J.
Bacteria use type IV secretion systems (T4SS) to translocate DNA and protein substrates to target cells of phylogenetically diverse taxa. Recently, by use of an assay termed transfer DNA...
Christie, Peter J., Hahn, Matthias, Walbot, Virginia
Low-temperature stress was shown to cause a rapid increase in steady-state levels of alcohol dehydrogenase-1 message (Adh1) and protein activity (ADH1) in maize (Zea mays) (B37N, A188) and rice...
Jakubowski, Simon J., Cascales, Eric, Krishnamoorthy, Vidhya, Christie, Peter J.
Agrobacterium tumefaciens translocates DNA and protein substrates between cells via a type IV secretion system (T4SS) whose channel subunits include the VirD4 coupling protein, VirB11 ATPase, VirB6,...
Bayliss, Richard, Harris, Richard, Coutte, Loic, Monier, Amy, Fronzes, Remi, Christie, Peter J., ...
Type IV secretion (T4S) systems translocate DNA and protein effectors through the double membrane of Gram-negative bacteria. The paradigmatic T4S system in Agrobacterium tumefaciens is assembled from...
Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions
Atmakuri, Krishnamohan, Cascales, Eric, Burton, Oliver T, Banta, Lois M, Christie, Peter J
Agrobacterium tumefaciens translocates T-DNA through a polar VirB/D4 type IV secretion (T4S) system. VirC1, a factor required for efficient T-DNA transfer, bears a deviant Walker A and other sequence...
Chen, Yuqing, Zhang, Xiaolin, Manias, Dawn, Yeo, Hye-Jeong, Dunny, Gary M., Christie, Peter J.
Upon sensing of peptide pheromone, Enterococcus faecalis efficiently transfers plasmid pCF10 through a type IV secretion (T4S) system to recipient cells. The PcfF accessory factor and PcfG relaxase...