Fang, Tzan-Wei, Yang, Dazhi, Lewin, Nancy E, Marquez, Victor E, ...
Although protein kinase D (PKD), like protein kinase C (PKC), possesses a C1 domain that binds phorbol esters and diacylglycerol, the structural differences from PKC within this and other domains of...
Ács, Péter, Beheshti, Maryam, Szállási, Zoltán, Li, Luowei, Yuspa, Stuart H., Blumberg, Peter M.
Tyrosine phosphorylation has emerged as an important mechanism in the regulation of enzyme function. In this paper, we describe a mutant of PKCδ altered at a single tyrosine residue which has the...
Szallasi, Zoltan, Krausz, Kristopher W., Blumberg, Peter M.
In previous experiments, pretreatment of CD-1 mouse skin with prostratin (12-deoxyphorbol 13-acetate) inhibited hyperplasia, induction of ornithine decarboxylase and edema in response to acute...
Hennings, Henry, Blumberg, Peter M., Pettit, George R., Herald, Cherry L., Shores, Robert, Yuspa, Stuart H.
Bryostatin 1, like the phorbol esters, activates protein kinase C. However, bryostatin 1 induces only some of the effects in cultured cells which result from phorbol ester treatment, whereas it...
Sharkey, Nancy A., Blumberg, Peter M.
Diglycerides function as analogs of the phorbol ester tumor promoters. We compare here the activity of glycerol 1-myristate 2-acetate (GMA) with the corresponding phorbol 12-myristate 13-acetate...
Little, John B., Nagasawa, Hatsumi, Weichselbaum, Ralph R., Lovett, David R., Schmit, Ann, Kochenburger, Richard J., ...
Inhibition of colony formation by the phorbol ester tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA) over a wide range of concentrations (10−4–102 ng/ml) was examined in two normal...
Dunphy, William G., Lau, Ching-Ching, Blumberg, Peter M.
We have examined the activity of nine phorbol-related diterpene esters for stimulation of 2-deoxyglucose uptake in the Swiss 3T3 mouse cell line. The half-maximally effective doses (ED50's) of these...
Mezey, Éva, Tóth, Zsuzsanna E., Cortright, Daniel N., Arzubi, Michelle K., Krause, James E., Elde, Robert, ...
The cloned vanilloid receptor VR1 has attracted recent attention as a molecular integrator of painful stimuli on primary sensory neurons. The existence of vanilloid-sensitive neurons in the brain is,...
Caloca, Maria José, Garcia-Bermejo, Maria Laura, Blumberg, Peter M., Lewin, Nancy E., Kremmer, Elisabeth, Mischak, Harald, ...
The members of the chimaerin family of Rac-GTPase-activating proteins possess a single C1 domain with high homology to those present in protein kinase C (PKC) isozymes. This domain in PKCs is...
Li, Luowei, Lorenzo, Patricia S., Bogi, Krisztina, Blumberg, Peter M., Yuspa, Stuart H.
Inactivation of protein kinase Cδ (PKCδ) is associated with resistance to terminal cell death in epidermal tumor cells, suggesting that activation of PKCδ in normal epidermis may be a component of...
Blass, Michal, Kronfeld, Ilana, Kazimirsky, Gila, Blumberg, Peter M., Brodie, Chaya
Protein kinase Cδ (PKCδ) is involved in the apoptosis of various cells in response to diverse stimuli. In this study, we characterized the role of PKCδ in the apoptosis of C6 glioma cells in...
Rational Design of Drugs That Induce Human Immunodeficiency Virus Replication
Hamer, Dean H., Bocklandt, Sven, McHugh, Louise, Chun, Tae-Wook, Blumberg, Peter M., Sigano, Dina M., ...
Drugs that induce human immunodeficiency virus type 1 (HIV-1) replication could be used in combination with highly active antiretroviral therapy (HAART) to reduce the size of the latent reservoir...
Mechanism of Action and Development of Resistance to a New Amidino Penicillin
Matsuhashi, Sachiko, Kamiryo, Tatsuyuki, Blumberg, Peter M., Linnett, Paul, Willoughby, Eileen, Strominger, Jack L.
The mechanism of killing of Escherichia coli by a novel beta-lactam antibiotic, an amidino penicillin, has been investigated. This compound converts E. coli to relatively stable spherical forms at...
Storm, Daniel R., Blumberg, Peter M., Strominger, Jack L.
An insoluble penicillin derivative, 6-aminopenicillanic acid covalently coupled to Sepharose, was used to investigate the location of the d-alanine carboxypeptidase in the Bacillus subtilis membrane....
Penicillin Binding Components in Bacillius subtilis During Sporulation
Anwar, Rashid A., Blumberg, Peter M., Strominger, Jack L.
The penicillin binding components of sporulating Bacillus subtilis 3610 were compared with those of the vegetative organism. No qualitative difference in binding components was observed. The total...
d-Alanine Carboxypeptidase and Cell Wall Cross-Linking in Bacillus subtilis
Sharpe, Arlene, Blumberg, Peter M., Strominger, Jack L.
Inhibition of d-alanine carboxypeptidase in Bacillus subtilis by 95% caused no measurable change in the degree of cross-linking of the peptidoglycan.
Specific binding of phorbol ester tumor promoters
Driedger, Paul E., Blumberg, Peter M.
[20-3H]Phorbol 12,13-dibutyrate bound to particulate preparations from chicken embryo fibroblasts in a specific, saturable, reversible fashion. Equilibrium binding occurred with a Kd of 25 nM; this...
Blumberg, Peter M., Strominger, Jack L.
The D-alanine carboxypeptidase of Bacillus subtilis is a particulate enzyme that is irreversibly inactivated by penicillins and cephalosporins. However, the lethal concentrations of these antibiotics...
Blumberg, Peter M., Strominger, Jack L.
An affinity chromatography technique was developed to isolate the five penicillin-binding components present in Bacillus subtilis membranes. The proteins were solubilized by the detergent Nonidet...
Mezey, Éva, Tóth, Zsuzsanna E., Cortright, Daniel N., Arzubi, Michelle K., Krause, James E., Elde, Robert, ...
The cloned vanilloid receptor VR1 has attracted recent attention as a molecular integrator of painful stimuli on primary sensory neurons. The existence of vanilloid-sensitive neurons in the brain is,...
Caloca, Maria José, Garcia-Bermejo, Maria Laura, Blumberg, Peter M., Lewin, Nancy E., Kremmer, Elisabeth, Mischak, Harald, ...
The members of the chimaerin family of Rac-GTPase-activating proteins possess a single C1 domain with high homology to those present in protein kinase C (PKC) isozymes. This domain in PKCs is...
Li, Luowei, Lorenzo, Patricia S., Bogi, Krisztina, Blumberg, Peter M., Yuspa, Stuart H.
Inactivation of protein kinase Cδ (PKCδ) is associated with resistance to terminal cell death in epidermal tumor cells, suggesting that activation of PKCδ in normal epidermis may be a component of...
Blass, Michal, Kronfeld, Ilana, Kazimirsky, Gila, Blumberg, Peter M., Brodie, Chaya
Protein kinase Cδ (PKCδ) is involved in the apoptosis of various cells in response to diverse stimuli. In this study, we characterized the role of PKCδ in the apoptosis of C6 glioma cells in...
Rational Design of Drugs That Induce Human Immunodeficiency Virus Replication
Hamer, Dean H., Bocklandt, Sven, McHugh, Louise, Chun, Tae-Wook, Blumberg, Peter M., Sigano, Dina M., ...
Drugs that induce human immunodeficiency virus type 1 (HIV-1) replication could be used in combination with highly active antiretroviral therapy (HAART) to reduce the size of the latent reservoir...
Mechanism of Action and Development of Resistance to a New Amidino Penicillin
Matsuhashi, Sachiko, Kamiryo, Tatsuyuki, Blumberg, Peter M., Linnett, Paul, Willoughby, Eileen, Strominger, Jack L.
The mechanism of killing of Escherichia coli by a novel beta-lactam antibiotic, an amidino penicillin, has been investigated. This compound converts E. coli to relatively stable spherical forms at...
Storm, Daniel R., Blumberg, Peter M., Strominger, Jack L.
An insoluble penicillin derivative, 6-aminopenicillanic acid covalently coupled to Sepharose, was used to investigate the location of the d-alanine carboxypeptidase in the Bacillus subtilis membrane....
Penicillin Binding Components in Bacillius subtilis During Sporulation
Anwar, Rashid A., Blumberg, Peter M., Strominger, Jack L.
The penicillin binding components of sporulating Bacillus subtilis 3610 were compared with those of the vegetative organism. No qualitative difference in binding components was observed. The total...
d-Alanine Carboxypeptidase and Cell Wall Cross-Linking in Bacillus subtilis
Sharpe, Arlene, Blumberg, Peter M., Strominger, Jack L.
Inhibition of d-alanine carboxypeptidase in Bacillus subtilis by 95% caused no measurable change in the degree of cross-linking of the peptidoglycan.
Specific binding of phorbol ester tumor promoters
Driedger, Paul E., Blumberg, Peter M.
[20-3H]Phorbol 12,13-dibutyrate bound to particulate preparations from chicken embryo fibroblasts in a specific, saturable, reversible fashion. Equilibrium binding occurred with a Kd of 25 nM; this...
Blumberg, Peter M., Strominger, Jack L.
The D-alanine carboxypeptidase of Bacillus subtilis is a particulate enzyme that is irreversibly inactivated by penicillins and cephalosporins. However, the lethal concentrations of these antibiotics...
Blumberg, Peter M., Strominger, Jack L.
An affinity chromatography technique was developed to isolate the five penicillin-binding components present in Bacillus subtilis membranes. The proteins were solubilized by the detergent Nonidet...
Wnts induce migration and invasion of myeloma plasma cells
Qiang, Ya-Wei, Walsh, Katie, Yao, Lei, Kedei, Noemi, Blumberg, Peter M., Rubin, Jeffrey S., ...
Multiple myeloma is an incurable form of lymphoid cancer characterized by accumulation of neoplastic plasma cells in the bone marrow cavity. Little is known about the mechanisms regulating myeloma...
Choi, Sung Hee, Czifra, Gabriella, Kedei, Noemi, Lewin, Nancy E., Lazar, Jozsef, Pu, Yongmei, ...
C1 domains mediate the recognition and subsequent signaling response to diacylglycerol and phorbol esters by protein kinase C (PKC) and by several other families of signal-transducing proteins such...
Lomonaco, Stephanie L., Kahana, Sarit, Blass, Michal, Brody, Yehuda, Okhrimenko, Hana, Xiang, Cunli, ...
The mechanism underlying the important role of protein kinase Cδ (PKCδ) in the apoptotic effect of etoposide in glioma cells is incompletely understood. Here, we examined the role of PKCδ in the...