Pike, Jason E., Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.
We have developed a system to reconstitute all of the proposed steps of Okazaki fragment processing using purified yeast proteins and model substrates. DNA polymerase δ was shown to extend an...
Stone, Jana E., Kissling, Grace E., Lujan, Scott A., Rogozin, Igor B., Stith, Carrie M., Burgers, Peter M. J., ...
To probe Pol ζ functions in vivo via its error signature, here we report the properties of Saccharomyces cerevisiae Pol ζ in which phenyalanine was substituted for the conserved Leu-979 in the...
Rossi, Marie L., Pike, Jason E., Wang, Wensheng, Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.
Eukaryotic Okazaki fragment maturation requires complete removal of the initiating RNA primer before ligation occurs. Polymerase delta (Pol delta) extends the upstream Okazaki fragment and displaces...
Rossi, Marie L., Pike, Jason E., Wang, Wensheng, Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.
Eukaryotic Okazaki fragment maturation requires complete removal of the initiating RNA primer before ligation occurs. Polymerase delta (Pol delta) extends the upstream Okazaki fragment and displaces...
Sommer, Debbie, Stith, Carrie M., Burgers, Peter M. J., Lahue, Robert S.
Small looped mispairs are corrected by DNA mismatch repair. In addition, a distinct process called large loop repair (LLR) corrects heteroduplexes up to several hundred nucleotides in bacteria, yeast...
Wardle, Josephine, Burgers, Peter M. J., Cann, Isaac K. O., Darley, Kate, Heslop, Pauline, Johansson, Erik, ...
Family B DNA polymerases from archaea such as Pyrococcus furiosus, which live at temperatures ∼100°C, specifically recognize uracil in DNA templates and stall replication in response to this base....
The fidelity of DNA synthesis by yeast DNA polymerase zeta alone and with accessory proteins (2006)
Zhong, Xuejun, Garg, Parie, Stith, Carrie M., McElhinny, Stephanie A. Nick, Kissling, Grace E., Burgers, Peter M. J., ...
DNA polymerase zeta (pol ζ) participates in several DNA transactions in eukaryotic cells that increase spontaneous and damage-induced mutagenesis. To better understand this central role in...
RPA and PCNA suppress formation of large deletion errors by yeast DNA polymerase {delta} (2006)
Fortune, John M., Stith, Carrie M., Kissling, Grace E., Burgers, Peter M. J., Kunkel, Thomas A.
In fulfilling its biosynthetic roles in nuclear replication and in several types of repair, DNA polymerase δ (pol δ) is assisted by replication protein A (RPA), the single-stranded DNA-binding...
RPA and PCNA suppress formation of large deletion errors by yeast DNA polymerase {delta} (2006)
Fortune, John M., Stith, Carrie M., Kissling, Grace E., Burgers, Peter M. J., Kunkel, Thomas A.
In fulfilling its biosynthetic roles in nuclear replication and in several types of repair, DNA polymerase δ (pol δ) is assisted by replication protein A (RPA), the single-stranded DNA-binding...
The fidelity of DNA synthesis by yeast DNA polymerase zeta alone and with accessory proteins (2006)
Zhong, Xuejun, Garg, Parie, Stith, Carrie M., McElhinny, Stephanie A. Nick, Kissling, Grace E., Burgers, Peter M. J., ...
DNA polymerase zeta (pol ζ) participates in several DNA transactions in eukaryotic cells that increase spontaneous and damage-induced mutagenesis. To better understand this central role in...
RPA and PCNA suppress formation of large deletion errors by yeast DNA polymerase {delta} (2006)
Fortune, John M., Stith, Carrie M., Kissling, Grace E., Burgers, Peter M. J., Kunkel, Thomas A.
In fulfilling its biosynthetic roles in nuclear replication and in several types of repair, DNA polymerase δ (pol δ) is assisted by replication protein A (RPA), the single-stranded DNA-binding...
The fidelity of DNA synthesis by yeast DNA polymerase zeta alone and with accessory proteins (2006)
Zhong, Xuejun, Garg, Parie, Stith, Carrie M., McElhinny, Stephanie A. Nick, Kissling, Grace E., Burgers, Peter M. J., ...
DNA polymerase zeta (pol ζ) participates in several DNA transactions in eukaryotic cells that increase spontaneous and damage-induced mutagenesis. To better understand this central role in...
Enzymatic switching for efficient and accurate translesion DNA replication (2004)
McCulloch, Scott D., Kokoska, Robert J., Chilkova, Olga, Welch, Carrie M., Johansson, Erik, Burgers, Peter M. J., ...
When cyclobutane pyrimidine dimers stall DNA replication by DNA polymerase (Pol) δ or ε, a switch occurs to allow translesion synthesis by DNA polymerase η, followed by another switch that...
Corrette-Bennett, Stephanie E., Borgeson, Claudia, Sommer, Debbie, Burgers, Peter M. J., Lahue, Robert S.
Small looped mispairs are corrected by DNA mismatch repair (MMR). In addition, a distinct process called large loop repair (LLR) corrects loops up to several hundred nucleotides in extracts of...
Roles of yeast DNA polymerases {delta} and {zeta} and of Rev1 in the bypass of abasic sites (2001)
Haracska, Lajos, Unk, Ildiko, Johnson, Robert E., Johansson, Erik, Burgers, Peter M.J., Prakash, Satya, ...
Identification of the fifth subunit os Saccharomyces cerevisiae Replication Factor C (1995)
Gary, Sonja L., Burgers, Peter M.J.
Yeast replication factor C (RF-C) is a muttipolypeptide complex required for chromosomal DNA replication. Previously this complex was known to consist of at least four subunits. We here report the...
Human cyclin/PCNA (proliferating cell nuclear antigen) is structurally, functionally, and immunologically homologous to the calf thymus auxiliary protein for DNA polymerase δ. This auxiliary protein...
Jin, Yong Hwan, Obert, Robyn, Burgers, Peter M. J., Kunkel, Thomas A., Resnick, Michael A., Gordenin, Dmitry A.
Many DNA polymerases (Pol) have an intrinsic 3′→5′ exonuclease (Exo) activity which corrects polymerase errors and prevents mutations. We describe a role of the 3′→5′ Exo of Pol δ as a...
Unk, Ildiko, Haracska, Lajos, Gomes, Xavier V., Burgers, Peter M. J., Prakash, Louise, Prakash, Satya
The Apn2 protein of Saccharomyces cerevisiae contains 3′→5′ exonuclease and 3′-phosphodiesterase activities, and these activities function in the repair of DNA strand breaks that have...
Yeast Rad17/Mec3/Ddc1: A sliding clamp for the DNA damage checkpoint
Majka, Jerzy, Burgers, Peter M. J.
The Saccharomyces cerevisiae Rad24 and Rad17 checkpoint proteins are part of an early response to DNA damage in a signal transduction pathway leading to cell cycle arrest. Rad24 interacts with the...
Two modes of FEN1 binding to PCNA regulated by DNA
Gomes, Xavier V., Burgers, Peter M.J.
The FEN1 nuclease functions during Okazaki fragment maturation in the eukaryotic cell. Like many other proliferating cell nuclear antigen (PCNA)-binding proteins, FEN1 interacts with the interdomain...
Enzymatic switching for efficient and accurate translesion DNA replication
McCulloch, Scott D., Kokoska, Robert J., Chilkova, Olga, Welch, Carrie M., Johansson, Erik, Burgers, Peter M. J., ...
When cyclobutane pyrimidine dimers stall DNA replication by DNA polymerase (Pol) δ or ε, a switch occurs to allow translesion synthesis by DNA polymerase η, followed by another switch that allows...
Corrette-Bennett, Stephanie E., Borgeson, Claudia, Sommer, Debbie, Burgers, Peter M. J., Lahue, Robert S.
Small looped mispairs are corrected by DNA mismatch repair (MMR). In addition, a distinct process called large loop repair (LLR) corrects loops up to several hundred nucleotides in extracts of...
Replication Protein A-Directed Unloading of PCNA by the Ctf18 Cohesion Establishment Complex
Bylund, Göran O., Burgers, Peter M. J.
The replication clamp PCNA is loaded around DNA by replication factor C (RFC) and functions in DNA replication and repair. Regulated unloading of PCNA during the progression and termination of DNA...
Roles of yeast DNA polymerases δ and ζ and of Rev1 in the bypass of abasic sites
Haracska, Lajos, Unk, Ildiko, Johnson, Robert E., Johansson, Erik, Burgers, Peter M.J., Prakash, Satya, ...
Abasic (AP) sites are one of the most frequently formed lesions in DNA, and they present a strong block to continued synthesis by the replicative DNA machinery. Here we show efficient bypass of an AP...
Zhuang, Zhihao, Yoder, Bonita L., Burgers, Peter M. J., Benkovic, Stephen J.
Numerous proteins that function in DNA metabolic pathways are known to interact with the proliferating cell nuclear antigen (PCNA). The important function of PCNA in stimulating various cellular...
The fidelity of DNA synthesis by yeast DNA polymerase zeta alone and with accessory proteins
Zhong, Xuejun, Garg, Parie, Stith, Carrie M., McElhinny, Stephanie A. Nick, Kissling, Grace E., Burgers, Peter M. J., ...
DNA polymerase zeta (pol ζ) participates in several DNA transactions in eukaryotic cells that increase spontaneous and damage-induced mutagenesis. To better understand this central role in...
RPA and PCNA suppress formation of large deletion errors by yeast DNA polymerase δ
Fortune, John M., Stith, Carrie M., Kissling, Grace E., Burgers, Peter M. J., Kunkel, Thomas A.
In fulfilling its biosynthetic roles in nuclear replication and in several types of repair, DNA polymerase δ (pol δ) is assisted by replication protein A (RPA), the single-stranded DNA-binding...
Jin, Yong Hwan, Obert, Robyn, Burgers, Peter M. J., Kunkel, Thomas A., Resnick, Michael A., Gordenin, Dmitry A.
Many DNA polymerases (Pol) have an intrinsic 3′→5′ exonuclease (Exo) activity which corrects polymerase errors and prevents mutations. We describe a role of the 3′→5′ Exo of Pol δ as a...
Unk, Ildiko, Haracska, Lajos, Gomes, Xavier V., Burgers, Peter M. J., Prakash, Louise, Prakash, Satya
The Apn2 protein of Saccharomyces cerevisiae contains 3′→5′ exonuclease and 3′-phosphodiesterase activities, and these activities function in the repair of DNA strand breaks that have...
Yeast Rad17/Mec3/Ddc1: A sliding clamp for the DNA damage checkpoint
Majka, Jerzy, Burgers, Peter M. J.
The Saccharomyces cerevisiae Rad24 and Rad17 checkpoint proteins are part of an early response to DNA damage in a signal transduction pathway leading to cell cycle arrest. Rad24 interacts with the...
Roles of yeast DNA polymerases δ and ζ and of Rev1 in the bypass of abasic sites
Haracska, Lajos, Unk, Ildiko, Johnson, Robert E., Johansson, Erik, Burgers, Peter M.J., Prakash, Satya, ...
Abasic (AP) sites are one of the most frequently formed lesions in DNA, and they present a strong block to continued synthesis by the replicative DNA machinery. Here we show efficient bypass of an AP...
Two modes of FEN1 binding to PCNA regulated by DNA
Gomes, Xavier V., Burgers, Peter M.J.
The FEN1 nuclease functions during Okazaki fragment maturation in the eukaryotic cell. Like many other proliferating cell nuclear antigen (PCNA)-binding proteins, FEN1 interacts with the interdomain...
Enzymatic switching for efficient and accurate translesion DNA replication
McCulloch, Scott D., Kokoska, Robert J., Chilkova, Olga, Welch, Carrie M., Johansson, Erik, Burgers, Peter M. J., ...
When cyclobutane pyrimidine dimers stall DNA replication by DNA polymerase (Pol) δ or ε, a switch occurs to allow translesion synthesis by DNA polymerase η, followed by another switch that allows...
Corrette-Bennett, Stephanie E., Borgeson, Claudia, Sommer, Debbie, Burgers, Peter M. J., Lahue, Robert S.
Small looped mispairs are corrected by DNA mismatch repair (MMR). In addition, a distinct process called large loop repair (LLR) corrects loops up to several hundred nucleotides in extracts of...
Replication Protein A-Directed Unloading of PCNA by the Ctf18 Cohesion Establishment Complex
Bylund, Göran O., Burgers, Peter M. J.
The replication clamp PCNA is loaded around DNA by replication factor C (RFC) and functions in DNA replication and repair. Regulated unloading of PCNA during the progression and termination of DNA...
Zhuang, Zhihao, Yoder, Bonita L., Burgers, Peter M. J., Benkovic, Stephen J.
Numerous proteins that function in DNA metabolic pathways are known to interact with the proliferating cell nuclear antigen (PCNA). The important function of PCNA in stimulating various cellular...
The fidelity of DNA synthesis by yeast DNA polymerase zeta alone and with accessory proteins
Zhong, Xuejun, Garg, Parie, Stith, Carrie M., McElhinny, Stephanie A. Nick, Kissling, Grace E., Burgers, Peter M. J., ...
DNA polymerase zeta (pol ζ) participates in several DNA transactions in eukaryotic cells that increase spontaneous and damage-induced mutagenesis. To better understand this central role in...
RPA and PCNA suppress formation of large deletion errors by yeast DNA polymerase δ
Fortune, John M., Stith, Carrie M., Kissling, Grace E., Burgers, Peter M. J., Kunkel, Thomas A.
In fulfilling its biosynthetic roles in nuclear replication and in several types of repair, DNA polymerase δ (pol δ) is assisted by replication protein A (RPA), the single-stranded DNA-binding...
A novel function of DNA polymerase ζ regulated by PCNA
Northam, Matthew R, Garg, Parie, Baitin, Dmitri M, Burgers, Peter M J, Shcherbakova, Polina V
DNA polymerase ζ (Polζ) participates in translesion DNA synthesis and is involved in the generation of the majority of mutations induced by DNA damage. The mechanisms that license access of Polζ...
Wardle, Josephine, Burgers, Peter M. J., Cann, Isaac K. O., Darley, Kate, Heslop, Pauline, Johansson, Erik, ...
Family B DNA polymerases from archaea such as Pyrococcus furiosus, which live at temperatures ∼100°C, specifically recognize uracil in DNA templates and stall replication in response to this base....
Partial reconstitution of DNA large loop repair with purified proteins from Saccharomyces cerevisiae
Sommer, Debbie, Stith, Carrie M., Burgers, Peter M. J., Lahue, Robert S.
Small looped mispairs are corrected by DNA mismatch repair. In addition, a distinct process called large loop repair (LLR) corrects heteroduplexes up to several hundred nucleotides in bacteria, yeast...
Stone, Jana E., Kissling, Grace E., Lujan, Scott A., Rogozin, Igor B., Stith, Carrie M., Burgers, Peter M. J., ...
To probe Pol ζ functions in vivo via its error signature, here we report the properties of Saccharomyces cerevisiae Pol ζ in which phenyalanine was substituted for the conserved Leu-979 in the...
Rossi, Marie L., Pike, Jason E., Wang, Wensheng, Burgers, Peter M. J., Campbell, Judith L., Bambara, Robert A.
Eukaryotic Okazaki fragment maturation requires complete removal of the initiating RNA primer before ligation occurs. Polymerase δ (Pol δ) extends the upstream Okazaki fragment and displaces the...