Lövenklev, Maria, Artin, Ingrid, Hagberg, Oskar, Borch, Elisabeth, Holst, Elisabet, Rådström, Peter
The effects of carbon dioxide, sodium chloride, and sodium nitrite on type B botulinum neurotoxin (BoNT/B) gene (cntB) expression in nonproteolytic Clostridium botulinum were investigated in a...
Lövenklev, Maria, Holst, Elisabet, Borch, Elisabeth, Rådström, Peter
A quantitative reverse transcription-PCR (qRT-PCR) method was developed to monitor the relative expression of the type B botulinum neurotoxin (BoNT/B) gene (cntB) in Clostridium botulinum. The levels...
Diagnostic PCR: validation and sample preparation are two sides of the same coin (2004)
Hoorfar, Jeffrey, Wolffs, Petra, Rådström, Peter
Increased use of powerful PCR technology for the routine detection of pathogens has focused attention on the need for international validation and preparation of official non-commercial guidelines....
Pre-PCR processing strategies (2004)
Rådström, Peter, Lövenklev, Maria, Wolffs, Petra, Löfström, Charlotta, Knutsson, Rickard
A review describes the sample prepn. for biochem. methods, enrichment methods, immunol. methods, and phys. methods. The pretreatment of a complex biol. sample is crucial, and for successful PCR the...
Physiological function of the maltose operon regulator, MalR, in Lactococcus lactis (2002)
Andersson, Ulrika, Rådström, Peter
Abstract Background Maltose metabolism is initiated by an ATP-dependent permease system in Lactococcus lactis . The subsequent degradation of intracellular maltose is performed by the concerted...
Evaluation of selective enrichment PCR procedures for Yersinia enterocolitica. (2002)
Knutsson, Rickard, Blixt, Ylva, Grage, Halfdan, Borch, Elisabeth, Rådström, Peter
Four enrichment PCR protocols for detecting unlysed cells of pathogenic Yersinia enterocolitica were studied. First, the probability of detecting Y. enterocolitica cells of known concentrations by a...
Enhanced Exopolysaccharide Production by Metabolic Engineering of Streptococcus thermophilus. (2002)
Levander, Fredrik, Svensson, Malin, Rådström, Peter
It is possible that the low levels of production of exopolysaccharides (EPSs) by lactic acid bacteria could be improved by altering the levels of enzymes in the central metabolism that influence the...
Knutsson, Rickard, Löfström, Charlotta, Grage, Halfdan, Hoorfar, Jeffrey, Rådström, Peter
The performance of a 5' nuclease real-time PCR assay was studied to optimize an automated method of detection of preenriched Salmonella enterica cells in buffered peptone water (BPW). The...
Andersson, Ulrika, Rådström, Peter
Maltose and trehalose catabolic pathways are linked through their common enzyme, beta-phosphoglucomutase, and metabolite, beta-glucose 1-phosphate, in Lactococcus lactis. Maltose is degraded by the...
Levander, Fredrik, Svensson, Malin, Rådström, Peter
Abstract Background Exopolysaccharides (EPSs) produced by lactic acid bacteria are important for the texture of fermented foods and have received a great deal of interest recently. However, the low...
Lantz, Pär-Gunnar, Tjerneld, Folke, Hahn-Hägerdal, Bärbel, Rådström, Peter
An aqueous two-phase system, consisting of poly(ethylene glycol) (PEG) and dextran, was employed to separate polymerase chain reaction (PCR)-inhibitory substances from bacterial cells. The PCR...
Effects of Amplification Facilitators on Diagnostic PCR in the Presence of Blood, Feces, and Meat
The full potential of diagnostic PCR is limited, in part, by the presence of inhibitors in complex biological samples that reduce the amplification efficiency. Therefore, different pre-PCR treatments...
Purification and Characterization of PCR-Inhibitory Components in Blood Cells
Al-Soud, Waleed Abu, Rådström, Peter
In a recent study, immunoglobulin G in human plasma was identified as a major inhibitor of diagnostic PCR (W. Abu Al-Soud, L. J. Jönsson, and P. Rådström. J. Clin. Microbiol. 38:345–350, 2000)....
Al-Soud, Waleed Abu, Jönsson, Leif J., Rådström, Peter
A major inhibitor of diagnostic PCR in human plasma was identified and the mechanism of inhibition was characterized. Human blood was divided by centrifugation into buffy coat, plasma, platelets, and...
Levander, Fredrik, Rådström, Peter
To study the influence of phosphoglucomutase (PGM) activity on exopolysaccharide (EPS) synthesis in glucose- and lactose-growing Streptococcus thermophilus, a knockout PGM mutant and a strain with...
Physiological Role of β-Phosphoglucomutase in Lactococcus lactis
Levander, Fredrik, Andersson, Ulrika, Rådström, Peter
A β-phosphoglucomutase (β-PGM) mutant of Lactococcus lactis subsp. lactis ATCC 19435 was constructed using a minimal integration vector and double-crossover recombination. The mutant and the...
Dahlenborg, Maria, Borch, Elisabeth, Rådström, Peter
A specific and sensitive combined selection and enrichment PCR procedure was developed for the detection of Clostridium botulinum types B, E, and F in fecal samples from slaughtered pigs. Two...
The PCR is an extremely powerful method for detecting microorganisms. However, its full potential as a rapid detection method is limited by the inhibition of the thermostable DNA polymerase from...
Knutsson, Rickard, Löfström, Charlotta, Grage, Halfdan, Hoorfar, Jeffrey, Rådström, Peter
The performance of a 5′ nuclease real-time PCR assay was studied to optimize an automated method of detection of preenriched Salmonella enterica cells in buffered peptone water (BPW). The...
Enhanced Exopolysaccharide Production by Metabolic Engineering of Streptococcus thermophilus
Levander, Fredrik, Svensson, Malin, Rådström, Peter
It is possible that the low levels of production of exopolysaccharides (EPSs) by lactic acid bacteria could be improved by altering the levels of enzymes in the central metabolism that influence the...
Löfström, Charlotta, Knutsson, Rickard, Axelsson, Charlotta Engdahl, Rådström, Peter
A PCR procedure has been developed for routine analysis of viable Salmonella spp. in feed samples. The objective was to develop a simple PCR-compatible enrichment procedure to enable DNA...
Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR
Wolffs, Petra, Grage, Halfdan, Hagberg, Oskar, Rådström, Peter
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as...
Wolffs, Petra, Knutsson, Rickard, Norling, Börje, Rådström, Peter
The development of real-time PCR thermal cycles in the late 1990s has opened up the possibility of accurate quantification of microorganisms in clinical, environmental, and food samples. However, a...
Lövenklev, Maria, Holst, Elisabet, Borch, Elisabeth, Rådström, Peter
A quantitative reverse transcription-PCR (qRT-PCR) method was developed to monitor the relative expression of the type B botulinum neurotoxin (BoNT/B) gene (cntB) in Clostridium botulinum. The levels...
Lövenklev, Maria, Artin, Ingrid, Hagberg, Oskar, Borch, Elisabeth, Holst, Elisabet, Rådström, Peter
The effects of carbon dioxide, sodium chloride, and sodium nitrite on type B botulinum neurotoxin (BoNT/B) gene (cntB) expression in nonproteolytic Clostridium botulinum were investigated in a...
Svensson, Malin, Waak, Elisabet, Svensson, Ulla, Rådström, Peter
Altered levels of enzymes in the central carbon metabolism in Streptococcus thermophilus increased the exopolysaccharide (EPS) production 3.3 times over that of the parent strain. The influence of...
Wolffs, Petra, Norling, Börje, Hoorfar, Jeffrey, Griffiths, Mansel, Rådström, Peter
Real-time PCR is fast, sensitive, specific, and can deliver quantitative data; however, two disadvantages are that this technology is sensitive to inhibition by food and that it does not distinguish...
De Vin, Filip, Rådström, Peter, Herman, Lieve, De Vuyst, Luc
Lactose-limited fermentations of 49 dairy Streptococcus thermophilus strains revealed four distinct fermentation profiles with respect to galactose consumption after lactose depletion. All the...
Effects of Amplification Facilitators on Diagnostic PCR in the Presence of Blood, Feces, and Meat
The full potential of diagnostic PCR is limited, in part, by the presence of inhibitors in complex biological samples that reduce the amplification efficiency. Therefore, different pre-PCR treatments...
Purification and Characterization of PCR-Inhibitory Components in Blood Cells
Al-Soud, Waleed Abu, Rådström, Peter
In a recent study, immunoglobulin G in human plasma was identified as a major inhibitor of diagnostic PCR (W. Abu Al-Soud, L. J. Jönsson, and P. Rådström. J. Clin. Microbiol. 38:345–350, 2000)....
Al-Soud, Waleed Abu, Jönsson, Leif J., Rådström, Peter
A major inhibitor of diagnostic PCR in human plasma was identified and the mechanism of inhibition was characterized. Human blood was divided by centrifugation into buffy coat, plasma, platelets, and...
Levander, Fredrik, Rådström, Peter
To study the influence of phosphoglucomutase (PGM) activity on exopolysaccharide (EPS) synthesis in glucose- and lactose-growing Streptococcus thermophilus, a knockout PGM mutant and a strain with...
Physiological Role of β-Phosphoglucomutase in Lactococcus lactis
Levander, Fredrik, Andersson, Ulrika, Rådström, Peter
A β-phosphoglucomutase (β-PGM) mutant of Lactococcus lactis subsp. lactis ATCC 19435 was constructed using a minimal integration vector and double-crossover recombination. The mutant and the...
Dahlenborg, Maria, Borch, Elisabeth, Rådström, Peter
A specific and sensitive combined selection and enrichment PCR procedure was developed for the detection of Clostridium botulinum types B, E, and F in fecal samples from slaughtered pigs. Two...
The PCR is an extremely powerful method for detecting microorganisms. However, its full potential as a rapid detection method is limited by the inhibition of the thermostable DNA polymerase from...
Knutsson, Rickard, Löfström, Charlotta, Grage, Halfdan, Hoorfar, Jeffrey, Rådström, Peter
The performance of a 5′ nuclease real-time PCR assay was studied to optimize an automated method of detection of preenriched Salmonella enterica cells in buffered peptone water (BPW). The...
Enhanced Exopolysaccharide Production by Metabolic Engineering of Streptococcus thermophilus
Levander, Fredrik, Svensson, Malin, Rådström, Peter
It is possible that the low levels of production of exopolysaccharides (EPSs) by lactic acid bacteria could be improved by altering the levels of enzymes in the central metabolism that influence the...
Löfström, Charlotta, Knutsson, Rickard, Axelsson, Charlotta Engdahl, Rådström, Peter
A PCR procedure has been developed for routine analysis of viable Salmonella spp. in feed samples. The objective was to develop a simple PCR-compatible enrichment procedure to enable DNA...
Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR
Wolffs, Petra, Grage, Halfdan, Hagberg, Oskar, Rådström, Peter
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as...
Wolffs, Petra, Knutsson, Rickard, Norling, Börje, Rådström, Peter
The development of real-time PCR thermal cycles in the late 1990s has opened up the possibility of accurate quantification of microorganisms in clinical, environmental, and food samples. However, a...
Lövenklev, Maria, Holst, Elisabet, Borch, Elisabeth, Rådström, Peter
A quantitative reverse transcription-PCR (qRT-PCR) method was developed to monitor the relative expression of the type B botulinum neurotoxin (BoNT/B) gene (cntB) in Clostridium botulinum. The levels...
Lövenklev, Maria, Artin, Ingrid, Hagberg, Oskar, Borch, Elisabeth, Holst, Elisabet, Rådström, Peter
The effects of carbon dioxide, sodium chloride, and sodium nitrite on type B botulinum neurotoxin (BoNT/B) gene (cntB) expression in nonproteolytic Clostridium botulinum were investigated in a...
De Vin, Filip, Rådström, Peter, Herman, Lieve, De Vuyst, Luc
Lactose-limited fermentations of 49 dairy Streptococcus thermophilus strains revealed four distinct fermentation profiles with respect to galactose consumption after lactose depletion. All the...
Svensson, Malin, Waak, Elisabet, Svensson, Ulla, Rådström, Peter
Altered levels of enzymes in the central carbon metabolism in Streptococcus thermophilus increased the exopolysaccharide (EPS) production 3.3 times over that of the parent strain. The influence of...
Wolffs, Petra, Norling, Börje, Hoorfar, Jeffrey, Griffiths, Mansel, Rådström, Peter
Real-time PCR is fast, sensitive, specific, and can deliver quantitative data; however, two disadvantages are that this technology is sensitive to inhibition by food and that it does not distinguish...
First Case of Type E Wound Botulism Diagnosed Using Real-Time PCR▿
Artin, Ingrid, Björkman, Per, Cronqvist, Jonas, Rådström, Peter, Holst, Elisabet
Wound botulism is a growing problem among injecting drug users. The condition is often difficult to diagnose, with laboratory confirmation in only 50% of the cases. Here we present a real-time...
Årsköld, Emma, Lohmeier-Vogel, Elke, Cao, Rong, Roos, Stefan, Rådström, Peter
Metabolic flux analysis indicated that the heterofermentative Lactobacillus reuteri strain ATCC 55730 uses both the Embden-Meyerhof pathway (EMP) and phosphoketolase pathway (PKP) when glucose or...
Artin, Ingrid, Carter, Andrew T., Holst, Elisabet, Lövenklev, Maria, Mason, David R., Peck, Michael W., ...
Carbon dioxide is an antimicrobial gas commonly used in modified atmosphere packaging. In the present study, the effects of carbon dioxide on the growth of and neurotoxin production by nonproteolytic...