Genetic Analysis of the Capsular Biosynthetic Locus from All 90 Pneumococcal Serotypes (2006)
Stephen D. Bentley, David M. Aanensen, Angeliki Mavroidi, David Saunders, Ester Rabbinowitsch, Matthew Collins, ...
Several major invasive bacterial pathogens are encapsulated. Expression of a polysaccharide capsule is essential for survival in the blood, and thus for virulence, but also is a target for host...
Sex and virulence in Escherichia coli: an evolutionary perspective (2006)
Wirth, Thierry, Falush, Daniel, Lan, Ruiting, Colles, Frances, Mensa, Patience, Wieler, Lothar H., ...
Pathogenic Escherichia coli cause over 160 million cases of dysentery and one million deaths per year, whereas non-pathogenic E. coli constitute part of the normal intestinal flora of healthy mammals...
Ribotyping has been widely used to characterise the seventh pandemic clone including South American and O139 variants which appeared in 1991 and 1992 respectively. To reveal the molecular basis of...
Ribotyping has been widely used to characterise the seventh pandemic clone including South American and O139 variants which appeared in 1991 and 1992 respectively. To reveal the molecular basis of...
A Vibrio cholerae pathogenicity island associated with epidemic and pandemic strains
Karaolis, David K. R., Johnson, Judith A., Bailey, Camella C., Boedeker, Edgar C., Kaper, James B., Reeves, Peter R.
The bacterial species Vibrio cholerae includes harmless aquatic strains as well as strains capable of causing epidemics and global pandemics of cholera. While investigating the relationship between...
Pupo, Gulietta M., Lan, Ruiting, Reeves, Peter R.
The evolutionary relationships of 46 Shigella strains representing each of the serotypes belonging to the four traditional Shigella species (subgroups), Dysenteriae, Flexneri, Boydii, and Sonnei,...
Wang, Lei, Rothemund, Deborah, Curd, Heather, Reeves, Peter R.
Flagellar (H) antigens are mostly encoded by genes at the fliC locus in E. coli. We have sequenced 11 H7 fliC genes from Escherichia coli strains that belong to seven O serotypes. These sequences,...
O antigen is part of the lipopolysaccharide present in the outer membrane of gram-negative bacteria. Escherichia coli and Salmonella enterica each have many forms of O antigen, but only three are...
Byun, Roy, Elbourne, Liam D. H., Lan, Ruiting, Reeves, Peter R.
Studies of the Vibrio cholerae population, using molecular typing techniques, have shown the existence of several pathogenic clones, mainly sixth-pandemic, seventh-pandemic, and U.S. Gulf Coast...
Jiang, Sheng-Mei, Wang, Lei, Reeves, Peter R.
Capsular polysaccharide (CPS) is a major virulence factor in Streptococcus pneumoniae. CPS gene clusters of S. pneumoniae types 4, 6B, 8, and 18C were sequenced and compared with those of CPS types...
Comparison of Vibrio cholerae Pathogenicity Islands in Sixth and Seventh Pandemic Strains
Karaolis, David K. R., Lan, Ruiting, Kaper, James B., Reeves, Peter R.
Epidemic Vibrio cholerae strains possess a large cluster of essential virulence genes on the chromosome called the Vibrio pathogenicity island (VPI). The VPI contains the tcp gene cluster encoding...
Lan, Ruiting, Lumb, Brad, Ryan, David, Reeves, Peter R.
Three genes, ipgD, mxiC, and mxiA, all in the invasion region of the Shigella virulence plasmid, were sequenced from strains representing a range of Shigella serotypes and from two enteroinvasive...
Wang, Lei, Qu, Wenjia, Reeves, Peter R.
Shigella strains are in reality clones of Escherichia coli and are believed to have emerged relatively recently (G. M. Pupo, R. Lan, and P. R. Reeves, Proc. Natl. Acad. Sci. USA 97:10567–10572,...
Shepherd, James G., Wang, Lei, Reeves, Peter R.
Escherichia coli Sonnei has an O antigen identical to that of Plesiomonas shigelloides O17, and its O-antigen gene cluster is located on a plasmid. By sequencing the chromosomal O-antigen gene...
Sequencing of Escherichia coli O111 O-Antigen Gene Cluster and Identification of O111-Specific Genes
Wang, Lei, Curd, Heather, Qu, Wenjia, Reeves, Peter R.
Shiga toxin (Stx)-producing Escherichia coli strains of serogroup O111 are the most frequently isolated non-O157 strains causing outbreaks of gastroenteritis with hemolytic-uremic syndrome. The O111...
Andrianopoulos, Kanella, Wang, Lei, Reeves, Peter R.
GDP–l-fucose, the substrate for fucosyltransferases for addition of fucose to polysaccharides or glycoproteins in both procaryotes and eucaryotes, is made from GDP–d-mannose. l-Fucose is a...
Relationships among the O-Antigen Gene Clusters of Salmonella enterica Groups B, D1, D2, and D3
Curd, Heather, Liu, Dan, Reeves, Peter R.
The O antigen is an important cell wall antigen of gram-negative bacteria, and the genes responsible for its biosynthesis are located in a gene cluster. We have cloned and sequenced the DNA segment...
Luz, Sara Pérez, Rodríguez-Valera, Francisco, Lan, Ruiting, Reeves, Peter R.
The 16S-23S spacer regions of two ribosomal operons (rrnA and rrnE) have been sequenced in seven representatives of the Salmonella enterica subspecies. Isolated nucleotide substitutions were found at...
Franco, Agustin V., Liu, Dan, Reeves, Peter R.
The O antigen is a polymer with a repeated unit. The chain length in most Escherichia coli strains has a modal value of 10 to 18 O units, but other strains have higher or lower modal values. wzz...
Lai, Vincent, Wang, Lei, Reeves, Peter R.
O antigen is part of the lipopolysaccharide present in the outer membrane of gram-negative bacteria. The surface-exposed O antigen is subject to selection by the host immune system, which may account...
The O157:H7 clone of Escherichia coli, which causes major, often prolonged outbreaks of gastroenteritis with hemolytic-uremic syndrome (HUS) such as those in Japan, Scotland, and the United States...
Lan, Ruiting, Reeves, Peter R.
The seventh cholera pandemic started in 1961 and continues today. A collection of 45 seventh pandemic isolates of V. cholerae sampled over a 33-year period were analyzed by amplified fragment length...
Pacinelli, Elvia, Wang, Lei, Reeves, Peter R.
O antigen is part of the lipopolysaccharide present in the outer membrane of gram-negative bacteria and is highly polymorphic. In this study, we obtained sequences of the O-antigen gene clusters for...
Hu, Honghua, Lan, Ruiting, Reeves, Peter R.
Fluorescent amplified fragment length polymorphism (AFLP) was applied to 46 Salmonella enterica serovar Typhimurium isolates of Australian origin comprising nine phage types, by using the restriction...
Wang, Lei, Andrianopoulos, Kanella, Liu, Dan, Popoff, Michel Y., Reeves, Peter R.
The 46 serogroups of Salmonella enterica have different O-antigens, and each is thought to have a specific form of the O-antigen cluster. Comparison of the 145 serovars of serogroup B revealed much...
Wang, Lei, Huskic, Sandy, Cisterne, Adam, Rothemund, Deborah, Reeves, Peter R.
Escherichia coli O55 is an important antigen which is often associated with enteropathogenic E. coli clones. We sequenced the genes responsible for its synthesis and identified genes for O-antigen...
Species-Wide Variation in the Escherichia coli Flagellin (H-Antigen) Gene
Wang, Lei, Rothemund, Deborah, Curd, Heather, Reeves, Peter R.
Escherichia coli is a clonal species. The best-understood components of its clonal variation are the flagellar (H) and polysaccharide (O) antigens, both well documented since the mid-1930s because of...
Lan, Ruiting, Stevenson, Gordon, Reeves, Peter R.
All Shigella and enteroinvasive Escherichia coli (EIEC) strains carry a 230-kb virulence plasmid (pINV) which is essential for their invasiveness. There are two sequence forms, pINV A and pINV B, of...
Feng, Lu, Senchenkova, Sof'ya N., Yang, Jinghua, Shashkov, Alexander S., Tao, Jiang, Guo, Hongjie, ...
The structural and genetic organization of the Escherichia coli O52 O antigen was studied. As identified by sugar and methylation analysis and nuclear magnetic resonance spectroscopy, the O antigen...
Samuel, Gabrielle, Hogbin, John-Paul, Wang, Lei, Reeves, Peter R.
Escherichia coli O157, Salmonella enterica O30, and Citrobacter freundii F90 have identical O-antigen structures, as do E. coli O55 and S. enterica O50. The O-antigen gene cluster sequences for E....
Molecular Evolutionary Relationships of Enteroinvasive Escherichia coli and Shigella spp.
Lan, Ruiting, Alles, M. Chehani, Donohoe, Kathy, Martinez, Marina B., Reeves, Peter R.
Enteroinvasive Escherichia coli (EIEC), a distinctive pathogenic form of E. coli causing dysentery, is similar in many properties to bacteria placed in the four species of Shigella. Shigella has been...
Rezwan, Ferdousi, Lan, Ruiting, Reeves, Peter R.
The molecular basis of the loss of tryptophan utilization (indole-negative phenotype) of Shigella strains, in effect clones of Escherichia coli, was investigated. Analysis of the tna operon of 23...
Feng, Lu, Senchenkova, Sof'ya N., Tao, Jiang, Shashkov, Alexander S., Liu, Bin, Shevelev, Sergei D., ...
Enterohemorrhagic Escherichia coli O145 strains are emerging as causes of hemorrhagic colitis and hemolytic uremic syndrome. In this study, we present the structure of the E. coli O145 O antigen and...
Structural and Genetic Characterization of the Shigella boydii Type 10 and Type 6 O Antigens
Senchenkova, Sof'ya N., Feng, Lu, Yang, Jinghua, Shashkov, Alexander S., Cheng, Jiansong, Liu, Dan, ...
Comparison of the O antigens of Shigella boydii types 10 and 6 by chemical analysis and nuclear magnetic resonance spectroscopy showed that their structures are similar, with the only difference...
Evolutionary Changes of the flhDC Flagellar Master Operon in Shigella Strains
Tominaga, Akira, Lan, Ruiting, Reeves, Peter R.
Shigella strains are nonmotile. The master operon of flagellar synthesis, flhDC, was analyzed for genetic damage in 46 Shigella strains representing all known serotypes. In 11 strains (B1, B3, B6,...
Genetic Analysis of the Capsular Biosynthetic Locus from All 90 Pneumococcal Serotypes
Bentley, Stephen D, Aanensen, David M, Mavroidi, Angeliki, Saunders, David, Rabbinowitsch, Ester, Collins, Matthew, ...
Several major invasive bacterial pathogens are encapsulated. Expression of a polysaccharide capsule is essential for survival in the blood, and thus for virulence, but also is a target for host...
Hu, Honghua, Lan, Ruiting, Reeves, Peter R.
Serovar Typhimurium of Salmonella enterica is a model organism for studies of pathogenesis that exhibits phage-type variation and variation in host range and virulence, but in a recent study showed...
Sex and virulence in Escherichia coli: an evolutionary perspective
Wirth, Thierry, Falush, Daniel, Lan, Ruiting, Colles, Frances, Mensa, Patience, Wieler, Lothar H, ...
Pathogenic Escherichia coli cause over 160 million cases of dysentery and one million deaths per year, whereas non-pathogenic E. coli constitute part of the normal intestinal flora of healthy mammals...
Genetic Relatedness of the Streptococcus pneumoniae Capsular Biosynthetic Loci▿ †
Mavroidi, Angeliki, Aanensen, David M., Godoy, Daniel, Skovsted, Ian C., Kaltoft, Margit S., Reeves, Peter R., ...
Streptococcus pneumoniae (the pneumococcus) produces 1 of 91 capsular polysaccharides (CPS) that define the serotype. The cps loci of 88 pneumococcal serotypes whose CPS is synthesized by the...
Aanensen, David M., Mavroidi, Angeliki, Bentley, Stephen D., Reeves, Peter R., Spratt, Brian G.
The sequences of the capsular biosynthetic (cps) loci of 90 serotypes of Streptococcus pneumoniae have recently been determined. Bioinformatic procedures were used to predict the general functions of...
Stevenson, Gordon, Dieckelmann, Manuela, Reeves, Peter R.
We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle...