Peter Vandenabeele

Influence of Temperature and Backside Roughness on the Emissivity of Si Wafers During Rapid Thermal Processing. (9999)

Vandenabeele, Peter, Maex, Karen

The influence of temperature and roughness on the backside emissivity of Si wafers was studied. In situ measurements were done in two commercial rapid thermal processing systems. An experimental...

Immunogenic Cell Death, DAMPs and Anti-cancer Therapeutics: An Emerging Amalgamation (2009)

Nowis, Dominika, Golab, Jakub, Vandenabeele, Peter, Krysko, Dmitri V

Immunogenic profile of certain cancer cell death mechanisms has been transmuted by research published over a period of last few years and this change has been so drastic that a new (sub)class of...

Cell death in the skin (2009)

Hoste, Esther, Vandenabeele, Peter, Declercq, Wim CORA

The skin is the largest organ of the body and protects the organism against external physical, chemical and biological insults, such as wounding, ultraviolet radiation and micro-organisms. The...

Inhibition of spontaneous neutrophil apoptosis by parabutoporin acts independently of NADPH oxidase inhibition but by lipid raft-dependent stimulation of Akt (2009)

Berghe, Tom Vanden, Parthoens, Eef, Asselbergh, Bob, Vandenabeele, Peter

Neutrophil cell death plays a crucial role in neutrophil homeostasis and the resolution of inflammation. The superoxide-producing NADPH oxidase is involved in pathogen degradation and subsequent...

The Biodata toolbox for MATLAB (2009)

De Gussem, Kris, De Gelder, Joke, Vandenabeele, Peter, Moens, Luc

This paper describes "The Biodata toolbox for MATLAB". a free software toolbox for MATLAB. Biodata is the result of a PhD project in Raman spectroscopy and is available under the GNU Public License...

Use of Dendrograms of Slice Spectra as a New Graphical Tool for the Interpretation of Two-Dimensional Correlation Spectra (2009)

De Gussem, Kris, Vandenabeele, Peter, Verbeken, Annemieke, Moens, Luc

In this paper a procedure is established to identify spectral bands that show similar behavior in two-dimensional correlation spectra. Slice spectra of bands that are affected in a single spectral...

Reference database of Raman spectra of pharmaceutical excipients (2009)

De Veij, Marleen, Vandenabeele, Peter, De Beer, Thomas, Remon, Jean Paul, Moens, Luc

Raman spectroscopy has evolved into an important fast, direct and nondestructive technique in pharmaceutical analysis. Usually, the focus in this field is mainly on the active ingredients and not on...

Identification of protein binders in works of art by high-performance liquid chromatography-diode array detector analysis of their tryptic digests (2009)

Fremout, Wim, Sanyova, Jana, Saverwyns, Steven, Vandenabeele, Peter, Moens, Luc

Proteins in works of art are generally determined by the relative amounts of amino acids. This method, however, implies a loss of information on the protein structure and its modifications....

Towards the differentiation of non-treated and treated corundum minerals by ion-beam-induced luminescence and other complementary techniques (2009)

Del Castillo, Calvo H., Deprez, N., Dupuis, T., Mathis, F., Deneckere, Annelien, Vandenabeele, Peter, ...

Differentiation of treated and non-treated gemstones is a chief concern for major jewellery import companies. Low-quality corundum specimens coming from Asia appear to be often treated with heat, BeO...

Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity Conditions (2009)

De Gelder, Joke, Vandenabeele, Peter, De Boever, Patrick, Mergeay, Max, Moens, Luc, De Vos, Paul

In this study, the effect of low-shear microgravity on the metabolism of Cupriavidus metallidurans LMG 1195 was studied with Raman spectroscopy. Therefore, the strain was cultured for 24 or 48 h in a...

First finding of burkeite in melt inclusions in olivine from sheared lherzolite xenoliths (2009)

Korsakov, Andrey V., Golovin, Alexander V., De Gussem, Kris, Sharygin, Igor S., Vandenabeele, Peter

For the first time burkeite was observed as a daughter phase in the melt inclusions in olivine by Raman spectroscopy. The olivine comes from sheared lherzolite xenoliths from the Udachnaya-East...

Multi-disciplinary investigation of the tomb of Menna (TT69), Theban Necropolis, Egypt (2009)

Vandenabeele, Peter, Garcia-Mareno, Renata, Mathis, Francois, Leterme, Kerstin, Van Elslande, Elsa, Hocquet, Francois-Philippe, ...

The archaeometrical survey of the tomb of Menna (TT69), which took place in November-December 2007, is part of the extended research program that aims to study and preserve this tomb in all its...

SitePredicting the cleavage of proteinase substrates (2009)

Verspurten, Jelle, Gevaert, Kris, Declercq, Wim, Vandenabeele, Peter

Proteinases are enzymes that play important roles in vital cellular and extracellular processes by hydrolytically cleaving peptide bonds in their protein substrates. This cleavage can be non-specific...

RIP Kinases at the Crossroads of Cell Death and Survival (2009)

Declercq, Wim, Vanden Berghe, Tom, Vandenabeele, Peter

Protein kinases of the receptor interacting protein (RIP) family collaborate with death receptor proteins to regulate cell death. Recent studies (Cho et al., 2009; He et al., 2009; Zhang et al.,...

Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes (2009)

Galluzzi, L, Aaronson, SA, Abrams, J, Alnemri, ES, Andrews, DW, Baehrecke, EH, ...

Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms...

Suppression of Interleukin-33 Bioactivity through Proteolysis by Apoptotic Caspases (2009)

Luthi, AU, Cullen, SP, McNeela, EA, Duriez, PJ, Afonina, IS, Sheridan, C, ...

Interleukin-33 (IL-33) is amember of the IL-1 family and is involved in polarization of T cells toward a T helper 2 (Th2) cell phenotype. IL-33 is thought to be activated via caspase-1-dependent...

The emerging roles of serine protease cascades in the epidermis (2009)

Ovaere, Petra, Lippens, Saskia, Vandenabeele, Peter, Declercq, Wim

It has become clear in recent years that serine proteases have an important role in epidermal homeostasis, and the signaling cascades are gradually being identified. For example, matriptase,...

Raman Spectroscopic Analysis of Cupriavidus metallidurans LMG 1195 (CH34) Cultured in Low-shear Microgravity (2008)

De Gelder, Joke, Vandenabeele, Peter, De Boever, Patrick, Mergeay, Max, Moens, Luc, De Vos, Paul

In this study, the effect of low-shear microgravity on the metabolism of Cupriavidus metallidurans LMG 1195 was studied with Raman spectroscopy. Therefore, the strain was cultured for 24 or 48 h in a...

NIR-FT-Raman spectroscopic analytical characterization of the fruits, seeds, and phytotherapeutic oils from rosehips (2008)

Da Silva, Carlos Eduardo, Vandenabeele, Peter, Edwards, Howell G. M., Cappa De Oliveira, Luiz Fernando

In this study nondestructive Raman spectroscopic analysis of rosehips has been conducted by laser excitation at 1064 nm, with the objective of direct measurement of different parts of the fruit,...

In vivo imaging of NF-kappa B activity during Escherichia coli-induced mammary gland infection (2008)

Notebaert, Sofie, Carlsen, Harald, Janssen, Daniel, Vandenabeele, Peter, Blomhoff, Rune, Meyer, Evelyne

In mammary gland infections, the contribution of NF-kappa B is not well defined, and was therefore investigated following intramammary inoculation of Escherichia coli. Non-invasive real-time in vivo...

Inflammatory mediators in Escherichia coli-induced mastitis in mice (2008)

Notebaert, Sofie, Demon, Dieter, Vanden Berghe, Tom, Vandenabeele, Peter, Meyer, Evelyne

Escherichia coli (E coli) infections in mouse mammary glands are rarely described and poorly characterized. In order to investigate the host immune response during coliform mastitis, several...

Tryptic Peptide Analysis of Protein Binders in Works of Art by Liquid Chromatography – Tandem Mass Spectrometry (2008)

Fremout, Wim, Dhaenens, Maarten, Saverwyns, S., Sanyova, J., Vandenabeele, Peter, Deforce, Dieter, ...

Comprehension of the materials, such as binding media, used by artists is of uttermost importance in restoration and in art historical studies. The most frequently used binders are drying oils and...

Caspase-mediated cleavage of the exosome subunit PM/Scl-75 during apoptosis (2007)

Schilders, Geurt, Raijmakers, Reinout, Malmegrim, Kelen CR, Walle, Lieselotte, Saelens, Xavier, Vree Egberts, Wilma, ...

Abstract Recent studies have implicated the dying cell as a potential reservoir of modified autoantigens that might initiate and drive systemic autoimmunity in susceptible hosts. A number of subunits...

Raman spectroscopy : new light on ancient artefacts (2007)

Vandenabeele, Peter, Moens, Luc

Raman spectroscopy, being a laser spectroscopic method, is gaining increasingly more interest for applications in the field of art and archaeology. The technique is especially appreciated for its...

Activation of p38 MAPK is required for Bax translocation to mitochondria, cytochrome c release and apoptosis induced by UVB irradiation in human keratinocytes (2004)

Van Laethem, An; U0041712, Van Kelst, Sofie, Lippens, Saskia, Declercq, Wim, Vandenabeele, Peter; U0004818, Janssens, Stefan, ...

This study establishes that activation of p38 MAPK by UVB represents a crucial signal required for the conformational change and translocation of Bax to the mitochondria in human keratinocytes....

Different Pathways Mediate Cytochrome c Release After Photodynamic Therapy with Hypericin (2001)

Annelies Vantieghem, Yan Xu, Wim Declercq, Peter Vandenabeele, Geertrui Denecker, Jackie R. Vandenheede, ...

In this study we show that overexpression of Bcl-2 in PC60R1R2 cells reveals a caspase-dependent mechanism of cytochrome c release following photodynamic therapy (PDT) with hypericin. Bcl-2...

Different pathways mediate cytochrome c release after photodynamic therapy with hypericin (2001)

Xu, Yan, Declerck, Wim, Vandenabeele, Peter, Denecker, Geertrui, ...

In this study we show that overexpression of Bcl-2 in PC60R1R2 cells reveals a caspase-dependent mechanism of cytochrome c release following photodynamic therapy (PDT) with hypericin. Bcl-2...

Phagocytosis of Necrotic Cells by Macrophages Is Phosphatidylserine Dependent and Does Not Induce Inflammatory Cytokine Production

Brouckaert, Greet, Kalai, Michael, Krysko, Dmitri V., Saelens, Xavier, Vercammen, Dominique, Ndlovu, `Matladi, ...

Apoptotic cells are cleared by phagocytosis during development, homeostasis, and pathology. However, it is still unclear how necrotic cells are removed. We compared the phagocytic uptake by...

Inhibition of apoptosis induced by ischemia-reperfusion prevents inflammation

Van ‘t Veer, Cornelis, Denecker, Geertrui, Heemskerk, Vincent H., Clauss, Matthias, ...

Ischemia followed by reperfusion leads to severe organ injury and dysfunction. Inflammation is considered to be the most important cause of tissue injury in organs subjected to ischemia. The...

The p55 tumour necrosis factor receptor TNFR1 contains a trans-Golgi network localization signal in the C-terminal region of its cytoplasmic tail.

Storey, Helen, Stewart, Abigail, Vandenabeele, Peter, Luzio, J Paul

It has been reported in some human cells that, in addition to a plasma membrane localization, members of the tumour necrosis factor receptor superfamily may be localized to the Golgi complex. We have...

Tumour necrosis factor-induced activation of c-Jun N-terminal kinase is sensitive to caspase-dependent modulation while activation of mitogen-activated protein kinase (MAPK) or p38 MAPK is not.

Mohamed, Ahmed A A, Jupp, Orla J, Anderson, Helen M, Littlejohn, Alison F, Vandenabeele, Peter, MacEwan, David J

The activation of the extracellular signal-regulated kinases (ERKs) by tumour necrosis factor-alpha (TNF) receptors (TNFRs) is an integral part of the cytokine's pleiotropic cellular responses. Here...

Distinct regulation of cytosolic phospholipase A2 phosphorylation, translocation, proteolysis and activation by tumour necrosis factor-receptor subtypes.

Jupp, Orla J, Vandenabeele, Peter, MacEwan, David J

The hormonally regulated Ca(2+)-dependent enzyme, cytosolic phospholipase A(2) (cPLA(2)) is activated by a range of inflammatory stimuli. Tumour necrosis factor-alpha (TNF) is one of the first known...

Phagocytosis of Necrotic Cells by Macrophages Is Phosphatidylserine Dependent and Does Not Induce Inflammatory Cytokine Production

Brouckaert, Greet, Kalai, Michael, Krysko, Dmitri V., Saelens, Xavier, Vercammen, Dominique, Ndlovu, `Matladi, ...

Apoptotic cells are cleared by phagocytosis during development, homeostasis, and pathology. However, it is still unclear how necrotic cells are removed. We compared the phagocytic uptake by...

Inhibition of apoptosis induced by ischemia-reperfusion prevents inflammation

Van ‘t Veer, Cornelis, Denecker, Geertrui, Heemskerk, Vincent H., Clauss, Matthias, ...

Ischemia followed by reperfusion leads to severe organ injury and dysfunction. Inflammation is considered to be the most important cause of tissue injury in organs subjected to ischemia. The...

The p55 tumour necrosis factor receptor TNFR1 contains a trans-Golgi network localization signal in the C-terminal region of its cytoplasmic tail.

Storey, Helen, Stewart, Abigail, Vandenabeele, Peter, Luzio, J Paul

It has been reported in some human cells that, in addition to a plasma membrane localization, members of the tumour necrosis factor receptor superfamily may be localized to the Golgi complex. We have...

Tumour necrosis factor-induced activation of c-Jun N-terminal kinase is sensitive to caspase-dependent modulation while activation of mitogen-activated protein kinase (MAPK) or p38 MAPK is not.

Mohamed, Ahmed A A, Jupp, Orla J, Anderson, Helen M, Littlejohn, Alison F, Vandenabeele, Peter, MacEwan, David J

The activation of the extracellular signal-regulated kinases (ERKs) by tumour necrosis factor-alpha (TNF) receptors (TNFRs) is an integral part of the cytokine's pleiotropic cellular responses. Here...

Distinct regulation of cytosolic phospholipase A2 phosphorylation, translocation, proteolysis and activation by tumour necrosis factor-receptor subtypes.

Jupp, Orla J, Vandenabeele, Peter, MacEwan, David J

The hormonally regulated Ca(2+)-dependent enzyme, cytosolic phospholipase A(2) (cPLA(2)) is activated by a range of inflammatory stimuli. Tumour necrosis factor-alpha (TNF) is one of the first known...

Non-destructive analysis of museum objects by fibre-optic Raman spectroscopy

Vandenabeele, Peter, Tate, Jim, Moens, Luc

Raman spectroscopy is a versatile technique that has frequently been applied for the investigation of art objects. By using mobile Raman instrumentation it is possible to investigate the artworks...

Vitamin D3 Induces Caspase-14 Expression in Psoriatic Lesions and Enhances Caspase-14 Processing in Organotypic Skin Cultures

Lippens, Saskia, Kockx, Mark, Denecker, Geertrui, Knaapen, Michiel, Verheyen, An, Christiaen, Ruben, ...

Caspase-14 is a nonapoptotic caspase family member whose expression in the epidermis is confined to the suprabasal layers, which consist of differentiating keratinocytes. Proteolytic activation of...

Caspase-mediated cleavage of the exosome subunit PM/Scl-75 during apoptosis

Schilders, Geurt, Raijmakers, Reinout, Malmegrim, Kelen CR, Walle, Lieselotte Vande, Saelens, Xavier, Vree Egberts, Wilma, ...

Recent studies have implicated the dying cell as a potential reservoir of modified autoantigens that might initiate and drive systemic autoimmunity in susceptible hosts. A number of subunits of the...

Caspases leave the beaten track: caspase-mediated activation of NF-κB

Lamkanfi, Mohamed, Declercq, Wim, Vanden Berghe, Tom, Vandenabeele, Peter

The proteolytic activity of the cysteinyl aspartate–specific proteases, named caspases, mainly connotes their central role in apoptosis and inflammation. In this review we report on recent data on...

Protein synthesis persists during necrotic cell death

Saelens, Xavier, Festjens, Nele, Parthoens, Eef, Vanoverberghe, Isabel, Kalai, Michael, Van Kuppeveld, Frank, ...

Cell death is an intrinsic part of metazoan development and mammalian immune regulation. Whereas the molecular events orchestrating apoptosis have been characterized extensively, little is known...

Inhibition of Caspases Increases the Sensitivity of L929 Cells to Necrosis Mediated by Tumor Necrosis Factor

Vercammen, Dominique, Beyaert, Rudi, Denecker, Geertrui, Goossens, Vera, Van Loo, Geert, Declercq, Wim, ...

Murine L929 fibrosarcoma cells treated with tumor necrosis factor (TNF) rapidly die in a necrotic way, due to excessive formation of reactive oxygen intermediates. We investigated the role of...

A Caspase-activated Factor (CAF) Induces Mitochondrial Membrane Depolarization and Cytochrome c Release by a Nonproteolytic Mechanism

Steemans, Margino, Goossens, Vera, Van De Craen, Marc, Van Herreweghe, Franky, Vancompernolle, Katia, De Vos, Kurt, ...

It is well established that apoptosis is accompanied by activation of procaspases and by mitochondrial changes, such as decrease in mitochondrial transmembrane potential (ΔΨm) and release of...

Dual Signaling of the Fas Receptor: Initiation of Both Apoptotic and Necrotic Cell Death Pathways

Vercammen, Dominique, Brouckaert, Greet, Denecker, Geertrui, Van De Craen, Marc, Declercq, Wim, Fiers, Walter, ...

Murine L929 fibrosarcoma cells were transfected with the human Fas (APO-1/CD95) receptor, and the role of various caspases in Fas-mediated cell death was assessed. Proteolytic activation of...

Regulation of the expression and processing of caspase-12

Kalai, Michael, Lamkanfi, Mohamed, Denecker, Geertrui, Boogmans, Michael, Lippens, Saskia, Meeus, Ann, ...

Phylogenetic analysis clusters caspase-12 with the inflammatory caspases 1 and 11. We analyzed the expression of caspase-12 in mouse embryos, adult organs, and different cell types and tested the...

Are metacaspases caspases?

Vercammen, Dominique, Declercq, Wim, Vandenabeele, Peter, Van Breusegem, Frank

The identification of caspases as major regulators of apoptotic cell death in animals initiated a quest for homologous peptidases in other kingdoms. With the discovery of metacaspases in plants,...

Acute Modulations in Permeability Barrier Function Regulate Epidermal Cornification : Role of Caspase-14 and the Protease-Activated Receptor Type 2

Demerjian, Marianne, Hachem, Jean-Pierre, Tschachler, Erwin, Denecker, Geertrui, Declercq, Wim, Vandenabeele, Peter, ...

Stratum corneum comprises corneocytes, derived from outer stratum granulosum during terminal differentiation, embedded in a lipid-enriched extracellular matrix, secreted from epidermal lamellar...

Caspase-14 reveals its secrets

Denecker, Geertrui, Ovaere, Petra, Vandenabeele, Peter, Declercq, Wim

Caspase-14 is a unique member of the evolutionarily conserved family of cysteinyl aspartate–specific proteinases, which are mainly involved in inflammation and apoptosis. However, recent evidence...