Blanchfield, JT, Gallagher, OP, Cros, C, Lewis, RJ, Alewood, PF, Toth, I
Conotoxins are highly constrained peptide toxins that exhibit pharmaceutically relevant biological activities. We herein report the extent of absorption and profile of distribution of a native...
Conopeptide to drug: The development, structure and activity correlation of XEN2174 (2006)
Brust, A, Alewood, D, Colless, B, Palant, E, Patterson, B, Wilson, D, ...
Loughnan, M., Nicke, A., Jones, A., Schroeder, C. I., Nevin, S. T., Adams, D. J., ...
The venoms of predatory marine snails ( Conus spp.) contain diverse mixtures of peptide toxins with high potency and selectivity for a variety of voltage-gated and ligand-gated ion channels. Here we...
Identification of physiologically functional peptides in dairy products (2000)
Dionysius, DA, Marschke, RJ, Wood, AJ, Milne, J, Beattie, TR, Jiang, H, ...
A wide range of peptides produced from milk proteins have been demonstrated to produce a physiological response in model systems. These peptides may be released from intact proteins in the...
Hill, JM, Alewood, PF, Craik, DJ
The three-dimensional solution structure of conotoxin TVIIA, a 30-residue polypeptide from the venom of the piscivorous cone snail Conus tulipa, has been determined using 2D H-1 NMR spectroscopy....
The Human Genome Project and other major sequencing projects have rapidly provided a vast array of new protein sequences. In the postgenomic era, the physical form of many of these gene-encoded...
Cavanagh, AC, Greer, JM, Alewood, PF, Love, SG, Alewood, D, Mould, AW, ...
Early pregnancy factor (EPF) is a secreted protein with immunosuppressive and growth factor properties. During pregnancy, it appears in maternal serum within 6-24 h of fertilization, is present for...
Bansal, PS, Grieve, PA, Marschke, RJ, Daly, NL, Junius, K, Craik, DJ, ...
We have shown that 44 amino acid residues N-terminal segment of kappa-casein exhibits considerable a-helical structure. This prompted us to investigate the structures of the remaining segments of...
Bansal, PS, Grieve, PA, Marschke, RJ, Daly, NL, Mcghie, E, Craik, DJ, ...
Zhang, B, Harness, J, Somodevilla-Torres, MJ, Hillyard, NC, Mould, AW, Alewood, D, ...
Early pregnancy factor (EPF) is a secreted protein with immunosuppressive and growth factor properties. During pregnancy, it appears in maternal serum within 6-24 h of fertilization, is present for...
Preparation and characterization of polyclonal antibodies against human chaperonin 10 (2000)
Somodevilla-Torres, MJ, Hillyard, NC, Morton, H, Alewood, D, Halliday, JA, Alewood, PF, ...
Early pregnancy factor (EPF) has been identified as an extracellular homologue of chaperonin 10 (Cpn10), a heat shock protein that functions within the cell as a molecular chaperone. Here, we report...
The Human Genome Project and other major sequencing projects have rapidly provided a vast array of new protein sequences. In the postgenomic era, the physical form of many of these gene-encoded...
Accelerated chemical synthesis of peptides and small proteins (1999)
The chemical synthesis of peptides and small proteins is a powerful complementary strategy to recombinant protein overexpression and is widely used in structural biology, immunology, protein...
Broxton, NM, Down, JG, Gehrman, J, Alewood, PF, Satchell, DG, Livett, BG
The activity of alpha-conotoxin (alpha-CTX) lml, from the vermivorous marine snail Conus imperialis, has been studied on mammalian nicotinic receptors on bovine chromaffin cells and at the rat...
Watson, AA, Scanlon, M, McGeary, RP, Alewood, PF, Meutermans, WDF, Bourne, GT, ...
We have developed a new 4-alkoxybenzyl-derived linker that anchors the C-terminal amino acid to the resin through the alpha-nitrogen atom. The linker allows BOC solid-phase peptide assembly and...
Nielsen, KJ, Adams, DA, Alewood, PF, Lewis, RJ, Thomas, L, Schroeder, T, ...
The effects of chirality inversions of Tyr13 on the structure-activity relationships of omega-conotoxins MVIIA and MVIIC were examined using a combination of 2D H-1 NMR spectroscopy and radioligand...
Nielsen, KJ, Adams, D, Thomas, L, Bond, T, Alewood, PF, Craik, DJ, ...
The omega-conotoxins are a set of structurally related, four-loop, six cysteine containing peptides, that have a range of selectivities for different subtypes of the voltage-sensitive calcium channel...
Solution structure of alpha-conotoxin ImI by H-1 nuclear magnetic resonance (1999)
Gehrmann, J, Daly, NL, Alewood, PF, Craik, DJ
alpha-Conotoxin ImI derives from the venom of Conus imperialis and is the first and only small-peptide ligand that selectively binds to the neuronal alpha(7) homopentameric subtype of the nicotinic...
Accelerated chemical synthesis of peptides and small proteins (1999)
The chemical synthesis of peptides and small proteins is a powerful complementary strategy to recombinant protein overexpression and is widely used in structural biology, immunology, protein...
Bourne, GT, Meutermans, WDF, Alewood, PF, McGeary, RP, Scanlon, M, Watson, AA, ...
We have developed a new 4-alkoxybenzyl-derived linker that anchors the C-terminal amino acid to the resin through the alpha-nitrogen atom. The linker allows BOC solid-phase peptide assembly and...
Meutermans, WDF, Golding, SW, Bourne, GT, Miranda, LP, Dooley, MJ, Alewood, PF, ...
Cyclic peptides comprise a large and important class of biologically active molecules. They are generally synthesized through amide bond-forming reactions of the C- and N- termini under high dilution...
Lackmann, M, Harpur, AG, Oates, AC, Mann, RJ, Gabriel, A, Meutermans, W, ...
The basic framework for the JAK/STAT pathway is well documented. Recruitment of latent cytoplasmic STAT transcription factors to tyrosine phosphorylated docking sites on cytokine receptors and their...
Hu, SH, Loughnan, M, Miller, R, Weeks, CM, Blessing, RH, Alewood, PF, ...
Conotoxins are valuable probes of receptors and ion channels because of their small size and highly selective activity. alpha-Conotoxin EpI, a 16-residue peptide from the mollusk-hunting Conus...
p-cresol as a reversible acylium ion scavenger in solid-phase peptide synthesis (1998)
Miranda, LP, Jones, A, Meutermans, WDF, Alewood, PF
In this work we have defined the nature of the p-cresol and p-thiocresol adducts generated from acylium ions during HF cleavage, following contemporary Boc/benzyl solid-phase peptide synthesis....
Gehrmann, J, Alewood, PF, Craik, DJ
The three possible disulfide bonded isomers of alpha-conotoxin GI have been selectively synthesised and their structures determined by H-1 NMR spectroscopy. alpha-Conotoxin GI derives from the venom...
Hill, JM, Oomen, CJ, Miranda, LP, Bingham, JP, Alewood, PF, Craik, DJ
alpha-Conotoxin MII, a 16-residue polypeptide from the venom of the piscivorous cone snail Conus magus, is a potent and highly specific blocker of mammalian neuronal nicotinic acetylcholine receptors...
Role of the 6-20 disulfide bridge in the structure and activity of epidermal growth factor (1998)
Barnham, KJ, Torres, AM, Alewood, D, Alewood, PF, Domagala, T, Nice, EC, ...
Two synthetic analogues of murine epidermal. growth factor, [Abu6, 20] mEGF4-48 (where Abu denotes amino-butyric acid) and [G1, M3, K21, H40] mEGF1-48, have been investigated by NMR spectroscopy....
Hu, SH, Gehrmann, J, Alewood, PF, Craik, DJ, Martin, JL
Conotoxins are small, cysteine-rich peptides isolated from the venom of Conus spp. of predatory marine snails, which selectively target specific receptors and ion channels critical to the functioning...
Pallaghy, PK, Alewood, D, Alewood, PF, Norton, RS
The solution structure of robustoxin, the lethal neurotoxin from the Sydney funnel-web spider Atrax robustus, has been determined from 2D H-1 NMR data, Robustoxin is a polypeptide of 42 residues...
Scanlon, MJ, Naranjo, D, Thomas, L, Alewood, PF, Lewis, RJ, Craik, DJ
Background: kappa-PVIIA is a 27-residue polypeptide isolated from the venom of Conus purpurascens and is the first member of a new class of conotoxins that block potassium channels. By comparison to...
Synthesis of alpha-aspartyl-containing cyclic peptides (1997)
Meutermans, WDF, Alewood, PF, Bourne, GT, Hawkins, B, Smythe, ML
alpha-Aspartyl-containing cyclic pentapeptides were synthesised in high yields using a strategy that maintained fluorenylmethyl protection on the aspartic acid side chain during chain assembly, resin...
Hill, JM, Alewood, PF, Craik, DJ
Background: The venoms of Conus snails contain small, disulfide-rich inhibitors of voltage-dependent sodium channels. Conotoxin GS is a 34-residue polypeptide isolated from Conus geographus that...