Philip C. Hanna

Macrophage Responses to B. Anthracis (2006)

Hanna, Philip C.

The goal of these studies is to monitor changes occurring in host macrophages using a cell culture model of the early establishment stage of anthrax. Global changes in cultured murine macrophage...

Operon Prediction for Sequenced Bacterial Genomes without Experimental Information � † (2006)

Nicholas H. Bergman, Karla D. Passalacqua, Philip C. Hanna, Zhaohui S. Qin

Various computational approaches have been proposed for operon prediction, but most algorithms rely on experimental or functional data that are only available for a small subset of sequenced genomes....

Corresponding Author: (2006)

Nicholas H. Bergman, Karla D. Passalacqua, Philip C. Hanna, Zhaohui S. Qin, Nicholas H. Bergman, Ph. D

Various computational approaches have been proposed for operon prediction, but most algorithms rely on experimental or functional data that are only available for a small subset of sequenced genomes....

Coordinate Intracellular Expression of Salmonella Genes Induced during Infection

Heithoff, Douglas M., Conner, Christopher P., Hentschel, Ute, Govantes, Fernando, Hanna, Philip C., Mahan, Michael J.

Salmonella typhimurium in vivo-induced (ivi) genes were grouped by their coordinate behavior in response to a wide variety of environmental and genetic signals, including pH, Mg2+, Fe2+, and PhoPQ....

Macrophage-Enhanced Germination of Bacillus anthracis Endospores Requires gerS

Ireland, John A. W., Hanna, Philip C.

Germination of Bacillus anthracis Sterne and plasmidless Δ-Sterne endospores was dramatically enhanced in RAW264.7 macrophage-like cells, while germination of nonpathogenic Bacillus endospores was...

Amino Acid- and Purine Ribonucleoside-Induced Germination of Bacillus anthracis ΔSterne Endospores: gerS Mediates Responses to Aromatic Ring Structures

Ireland, John A. W., Hanna, Philip C.

Specific combinations of amino acids or purine ribonucleosides and amino acids are required for efficient germination of endospores of Bacillus anthracis ΔSterne, a plasmidless strain, at ligand...

Identification and Characterization of the gerH Operon of Bacillus anthracis Endospores: a Differential Role for Purine Nucleosides in Germination

Weiner, Matthew A., Read, Timothy D., Hanna, Philip C.

We identified a tri-cistronic operon, gerH, in Bacillus anthracis that is important for endospore germination triggered by two distinct germination response pathways termed inosine-His and...

Macrophage-Mediated Germination of Bacillus anthracis Endospores Requires the gerH Operon

Weiner, Matthew A., Hanna, Philip C.

The gerHabc operon of Bacillus anthracis, encoding a gerA-like family member of germinant sensors, was shown to be required for endospore germination in the presence of macrophages and in...

Formation and Composition of the Bacillus anthracis Endospore†

Liu, Hongbin, Bergman, Nicholas H., Thomason, Brendan, Shallom, Shamira, Hazen, Alyson, Crossno, Joseph, ...

The endospores of Bacillus anthracis are the infectious particles of anthrax. Spores are dormant bacterial morphotypes able to withstand harsh environments for decades, which contributes to their...

Murine Macrophage Transcriptional Responses to Bacillus anthracis Infection and Intoxication

Bergman, Nicholas H., Passalacqua, Karla D., Gaspard, Renee, Shetron-Rama, Lynne M., Quackenbush, John, Hanna, Philip C.

Interactions between Bacillus anthracis and host macrophages represent critical early events in anthrax pathogenesis, but their details are not clearly understood. Here we report the first genomewide...

Cytokine Response and Survival of Mice Immunized with an Adenovirus Expressing Bacillus anthracis Protective Antigen Domain 4

McConnell, Michael J., Hanna, Philip C., Imperiale, Michael J.

Adenovirus vectors are promising for use in vaccinating against potential agents of bioterrorism and emerging infections because of their proven safety in humans and their ability to elicit rapid...

Coordinate Intracellular Expression of Salmonella Genes Induced during Infection

Heithoff, Douglas M., Conner, Christopher P., Hentschel, Ute, Govantes, Fernando, Hanna, Philip C., Mahan, Michael J.

Salmonella typhimurium in vivo-induced (ivi) genes were grouped by their coordinate behavior in response to a wide variety of environmental and genetic signals, including pH, Mg2+, Fe2+, and PhoPQ....

Macrophage-Enhanced Germination of Bacillus anthracis Endospores Requires gerS

Ireland, John A. W., Hanna, Philip C.

Germination of Bacillus anthracis Sterne and plasmidless Δ-Sterne endospores was dramatically enhanced in RAW264.7 macrophage-like cells, while germination of nonpathogenic Bacillus endospores was...

Amino Acid- and Purine Ribonucleoside-Induced Germination of Bacillus anthracis ΔSterne Endospores: gerS Mediates Responses to Aromatic Ring Structures

Ireland, John A. W., Hanna, Philip C.

Specific combinations of amino acids or purine ribonucleosides and amino acids are required for efficient germination of endospores of Bacillus anthracis ΔSterne, a plasmidless strain, at ligand...

Identification and Characterization of the gerH Operon of Bacillus anthracis Endospores: a Differential Role for Purine Nucleosides in Germination

Weiner, Matthew A., Read, Timothy D., Hanna, Philip C.

We identified a tri-cistronic operon, gerH, in Bacillus anthracis that is important for endospore germination triggered by two distinct germination response pathways termed inosine-His and...

Macrophage-Mediated Germination of Bacillus anthracis Endospores Requires the gerH Operon

Weiner, Matthew A., Hanna, Philip C.

The gerHabc operon of Bacillus anthracis, encoding a gerA-like family member of germinant sensors, was shown to be required for endospore germination in the presence of macrophages and in...

Formation and Composition of the Bacillus anthracis Endospore†

Liu, Hongbin, Bergman, Nicholas H., Thomason, Brendan, Shallom, Shamira, Hazen, Alyson, Crossno, Joseph, ...

The endospores of Bacillus anthracis are the infectious particles of anthrax. Spores are dormant bacterial morphotypes able to withstand harsh environments for decades, which contributes to their...

Murine Macrophage Transcriptional Responses to Bacillus anthracis Infection and Intoxication

Bergman, Nicholas H., Passalacqua, Karla D., Gaspard, Renee, Shetron-Rama, Lynne M., Quackenbush, John, Hanna, Philip C.

Interactions between Bacillus anthracis and host macrophages represent critical early events in anthrax pathogenesis, but their details are not clearly understood. Here we report the first genomewide...

Cytokine Response and Survival of Mice Immunized with an Adenovirus Expressing Bacillus anthracis Protective Antigen Domain 4

McConnell, Michael J., Hanna, Philip C., Imperiale, Michael J.

Adenovirus vectors are promising for use in vaccinating against potential agents of bioterrorism and emerging infections because of their proven safety in humans and their ability to elicit rapid...

Transcriptional Profiling of the Bacillus anthracis Life Cycle In Vitro and an Implied Model for Regulation of Spore Formation†

Bergman, Nicholas H., Anderson, Erica C., Swenson, Ellen E., Niemeyer, Matthew M., Miyoshi, Amy D., Hanna, Philip C.

The life cycle of Bacillus anthracis includes both vegetative and endospore morphologies which alternate based on nutrient availability, and there is considerable evidence indicating that the ability...

Operon Prediction for Sequenced Bacterial Genomes without Experimental Information▿ †

Bergman, Nicholas H., Passalacqua, Karla D., Hanna, Philip C., Qin, Zhaohui S.

Various computational approaches have been proposed for operon prediction, but most algorithms rely on experimental or functional data that are only available for a small subset of sequenced genomes....

Biosynthetic Analysis of the Petrobactin Siderophore Pathway from Bacillus anthracis▿

Lee, Jung Yeop, Janes, Brian K., Passalacqua, Karla D., Pfleger, Brian F., Bergman, Nicholas H., Liu, Haichuan, ...

The asbABCDEF gene cluster from Bacillus anthracis is responsible for biosynthesis of petrobactin, a catecholate siderophore that functions in both iron acquisition and virulence in a murine model of...

The Global Transcriptional Responses of Bacillus anthracis Sterne (34F2) and a ΔsodA1 Mutant to Paraquat Reveal Metal Ion Homeostasis Imbalances during Endogenous Superoxide Stress▿ †

Passalacqua, Karla D., Bergman, Nicholas H., Lee, Jung Yeop, Sherman, David H., Hanna, Philip C.

Microarray analyses were conducted to evaluate the paraquat-induced global transcriptional response of Bacillus anthracis Sterne (34F2) to varying levels of endogenous superoxide stress. Data...

Transcriptional Profiling of Bacillus anthracis during Infection of Host Macrophages▿ †

Bergman, Nicholas H., Anderson, Erica C., Swenson, Ellen E., Janes, Brian K., Fisher, Nathan, Niemeyer, Matthew M., ...

The interaction between Bacillus anthracis and the mammalian phagocyte is one of the central stages in the progression of inhalational anthrax, and it is commonly believed that the host cell plays a...

The Type III Pantothenate Kinase Encoded by coaX Is Essential for Growth of Bacillus anthracis▿

Paige, Carleitta, Reid, Sean D., Hanna, Philip C., Claiborne, Al

In Bacillus anthracis, the novel type III pantothenate kinase (PanKBa; encoded by coaX) catalyzes the first committed step in coenzyme A biosynthesis. We have demonstrated by analyzing the growth...

Structural and functional analysis of AsbF: Origin of the stealth 3,4-dihydroxybenzoic acid subunit for petrobactin biosynthesis

Pfleger, Brian F., Kim, Youngchang, Nusca, Tyler D., Maltseva, Natalia, Lee, Jung Yeop, Rath, Christopher M., ...

Petrobactin, a virulence-associated siderophore produced by Bacillus anthracis, chelates ferric iron through the rare 3,4-isomer of dihydroxybenzoic acid (3,4-DHBA). Most catechol siderophores,...

Transcriptional Profiling of Bacillus anthracis Sterne (34F2) during Iron Starvation

Carlson, Paul E., Carr, Katherine A., Janes, Brian K., Anderson, Erica C., Hanna, Philip C.

Lack of available iron is one of many environmental challenges that a bacterium encounters during infection and adaptation to iron starvation is important for the pathogen to efficiently replicate...