Formin1 disruption confers oligodactylism and alters Bmp signaling (2009)
Zhou, Fen, Leder, Philip, Zuniga, Aimée, Dettenhofer, Markus
Proper limb development requires concerted communication between cells within the developing limb bud. Several molecules have been identified which contribute to the formation of a circuitry loop...
Structure and Expression of the Human Immunoglobulin X (2008)
Wedetermined theDNA sequence oftwo large regions ofchromosome 22: 33.7 kb containing the Ct, complex; and 5.2 kb 5 ' of the functionally rearranged X gene from the human myeloma, U266. Analysis...
Identifying genes preferentially expressed in undifferentiated embryonic stem cells (2007)
Abstract Background The mechanism involved in the maintenance and differentiation of embryonic stem (ES) cells is incompletely understood. Results To address this issue, we have developed a...
Leder, Aya, McMenamin, Jennifer, Fontaine, Karen, Bishop, Alexander, Leder, Philip
Thalassemia is a disease caused by a variety of mutations affecting both the adult and embryonic α- and β-globin loci. A mouse strain carrying an embryonic ζ-globin gene disrupted by the insertion...
Leder, Aya, McMenamin, Jennifer, Fontaine, Karen, Bishop, Alexander, Leder, Philip
Thalassemia is a disease caused by a variety of mutations affecting both the adult and embryonic α- and β-globin loci. A mouse strain carrying an embryonic ζ-globin gene disrupted by the insertion...
Recently, a family of stimulatory ligands for the EGF/erbB family of receptor tyrosine kinases has been described. This family includes Neu differentiation factor (NDF), heregulin (HRG),...
Cancer is a complex multi step disease and progresses through successive accumulation of genetic mutations. Loss of tumor suppressor gene p53 and activation of oncogene Neu/ErbB2 are among the most...
Cancer is a complex multistep disease and progresses through successive accumulation of genetic mutations. Loss of tumor suppressor gene p53 and activation of oncogene Neu/ErB2 are among the most...
Cancer develops through accumulation of multiple genetic mutations. Loss of tumor suppressor gene p53 and activation of oncogene Neu/ErbB2 are among the most frequent genetic alterations in human...
Rothenberg, Marc E., MacLean, James A., Pearlman, Eric, Luster, Andrew D., Leder, Philip
The chemokines are a large group of chemotactic cytokines that regulate leukocyte trafficking and have recently been shown to inhibit human immunodeficiency virus entry into cells. Eotaxin is a C-C...
Ornitz, David M., Cardiff, Robert D., Kuo, Ann, Leder, Philip
Background: Previous studies have shown associations between over-expresion of int-2 messenger RNA (mRNA) and murine mammary tumors and between amplification of the int-2 genomic locus and human...
In vitro and in vivo analysis of the c-myc RNA polymerase II promoter (1991)
Sussman, Daniel J., Chung, Jay, Leder, Philip
The c-myc promoter has the unusual property of displaying both RNA polymerase II (Pol II) and RNA polymerase III (Pol III) activities. Both Pol II and Pol III utilize the same transcription...
Complete nucleotide sequence of the membrane form of the human IgM heavy chain (1990)
Friedlander, Robert M., Nussenzweig, Michel C., Leder, Philip
Structure and expression of a human subgroup II immunoglobulin x gene (1986)
We have determined the entire nucleotide sequence of an active immunoglo-bulin Vk gene of the human subgroup II. Comparison of the main coding sequence of this gene to another member of this subgroup...
Moulding, Christopher, Rapoport, Aaron, Goldman, Paula, Battey, Jim, Lenoir, Gilbert M., Leder, Philip
The balanced translocations that occur between the c-myc and immunoglobulin loci in Burkitt lymphoma provide an unusual opportunity to analyze both products of a reciprocal recombination....
McBride, O. Wesley, Battey, James, Hollis, Gregory F., Swan, David C., Siebenlist, Ulrich, Leder, Philip
Analysis of a group of human/rodent somatic cell hybrids with nucleic acid probes prepared from cloned human variable region (VH), junctional (JH), and constant region (Cϵ) heavy chain...
Werner syndrome (WS) is an autosomal recessive disorder characterized by genomic instability and the premature onset of a number of age-related diseases. The gene responsible for WS encodes a member...
Pleiotropic defects in ataxia-telangiectasia protein-deficient mice
Elson, Ari, Wang, Yaoqi, Daugherty, Cathie J., Morton, Cynthia C., Zhou, Fen, Campos-Torres, Juanita, ...
We have generated a mouse model for ataxia-telangiectasia by using gene targeting to generate mice that do not express the Atm protein. Atm-deficient mice are retarded in growth, do not produce...
Wang, Y. Alan, Elson, Ari, Leder, Philip
Ataxia telangiectasia (AT) is an autosomal recessive disorder characterized by growth retardation, cerebellar ataxia, oculocutaneous telangiectasias, and a high incidence of lymphomas and leukemias....
A normal β-globin allele as a modifier gene ameliorating the severity of α-thalassemia in mice
Leder, Aya, Wiener, Edith, Lee, Matthew J., Wickramasinghe, Sunitha N., Leder, Philip
Thalassemia is a heritable human anemia caused by a variety of mutations that affect expression of the α- or the β-chain of hemoglobin. The expressivity of the phenotype is likely to be influenced...
Vollrath, Benedikt, Fitzgerald, Kevin J., Leder, Philip
The neurogenic gene brainiac was first isolated in Drosophila melanogaster, where it interacts genetically with members of the Notch signaling cascade. We have isolated a murine homologue of the...
Martin, Stuart S., Leder, Philip
Many tumor cells are impaired in adhesion-regulated apoptosis, which contributes to their metastatic potential. However, suppression of this apoptotic pathway in untransformed cells is not mediated...
Vollrath, Benedikt, Pudney, Jeffrey, Asa, Sylvia, Leder, Philip, Fitzgerald, Kevin
The Drosophila neuralized gene shows genetic interactions with Notch, Enhancer of split, and other neurogenic genes and is thought to be involved in cell fate specification in the central nervous...
Ecsedy, Jeffrey A., Michaelson, Jennifer S., Leder, Philip
We describe an interaction between homeodomain-interacting protein kinase 1 (HIPK1) and Daxx, two transcriptional regulators important in transducing growth-regulatory signals. We demonstrate that...
Critical Role for Mouse Hus1 in an S-Phase DNA Damage Cell Cycle Checkpoint
Weiss, Robert S., Leder, Philip, Vaziri, Cyrus
Mouse Hus1 encodes an evolutionarily conserved DNA damage response protein. In this study we examined how targeted deletion of Hus1 affects cell cycle checkpoint responses to genotoxic stress. Unlike...
Trupin, Joel S., Rottman, Fritz M., Brimacombe, Richard L. C., Leder, Philip, Bernfield, Merton R., Nirenberg, Marshall W.
CELL-FREE PEPTIDE SYNTHESIS DEPENDENT UPON SYNTHETIC OLIGODEOXYNUCLEOTIDES*
Leder, Philip, Clark, Brian F. C., Sly, William S., Pestka, Sidney, Nirenberg, Marshall W.
TRANSLOCATION OF MRNA CODONS, II. PROPERTIES OF AN ANTI-TRANSLOCASE ANTIBODY
Leder, Philip, Skogerson, Lawrence E., Roufa, Donald J.
A purified preparation of translocase, one of several enzymes required for protein biosynthesis, has been used to prepare a specific anti-translocase antibody. This antibody provides an extremely...
TRANSLOCATION OF MRNA CODONS, I. THE PREPARATION AND CHARACTERISTICS OF A HOMOGENEOUS ENZYME
Leder, Philip, Skogerson, Lawrence E., Nau, Marion M.
This report describes a convenient scheme for the further purification of an E. coli enzyme which is required for the translocation step in protein biosynthesis. The homogeneous enzyme translocase...
Inactivation of mouse Hus1 results in genomic instability and impaired responses to genotoxic stress
Weiss, Robert S., Enoch, Tamar, Leder, Philip
The eukaryotic cell cycle is overseen by regulatory mechanisms, termed checkpoints, that respond to DNA damage, mitotic spindle defects, and errors in the ordering of cell cycle events. The DNA...
Michaelson, Jennifer S., Bader, Debra, Kuo, Frank, Kozak, Christine, Leder, Philip
The mammalian Daxx gene has been identified in a diverse set of yeast interaction trap experiments. Although a facilitating role for Daxx in Fas-induced apoptosis has been suggested, Daxx’s...
Chester, Nicholas, Kuo, Frank, Kozak, Christine, O’Hara, Cathie D., Leder, Philip
Bloom’s syndrome is a human autosomal genetic disorder characterized at the cellular level by genome instability and increased sister chomatid exchanges (SCEs). Clinical features of the disease...
An Association Between Globin Messenger RNA and 60S RNA Derived from Friend Leukemia Virus
Ikawa, Yoji, Ross, Jeffrey, Leder, Philip
The association between certain cellular RNAs and purified RNA tumor viruses prompted us to examine the possibility that specific host messenger RNAs might also be incorporated into RNA tumor...
Characterization of the Initial Peptide of Qβ RNA Polymerase and Control of Its Synthesis
Skogerson, Lawrence, Roufa, Donald, Leder, Philip
Bacteriophage Qβ RNA directs the cell-free synthesis of two fMet-containing dipeptides prior to the first round of ribosomal translocation. One of these, fMet-Ala, corresponds to the initial segment...
Globin Messenger-RNA Induction During Erythroid Differentiation of Cultured Leukemia Cells
Ross, Jeffrey, Ikawa, Yoji, Leder, Philip
A cloned line of murine proerythroblastoid cells (T-3-Cl-2), transformed by Friend leukemia virus, undergoes changes associated with erythroid differentiation when treated with dimethylsulfoxide in...
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to...
In Vitro Synthesis of DNA Complementary to Purified Rabbit Globin mRNA
Ross, Jeffrey, Aviv, Haim, Scolnick, Edward, Leder, Philip
Several properties of the viral RNA-dependent DNA polymerases and of rabbit globin mRNA make it possible to consider synthesis of the globin gene in vitro. These enzymes copy an RNA template using a...
The Organization and Diversity of Immunoglobulin Genes*
Leder, Philip, Honjo, Tasuku, Packman, Seymour, Swan, David, Nau, Marion, Norman, Barbara
We have used purified mouse immunoglobulin light chain mRNA and synthetic DNA which is complementary to it to assess the reiteration frequency of gene sequences corresponding to the κ constant...
Enzymatic Synthesis of Solid Phase-Bound DNA Sequences Corresponding to Specific Mammalian Genes
Venetianer, Pal, Leder, Philip
With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid...
Direct induction of T lymphocyte-specific gene expression by the mammalian Notch signaling pathway
The Notch signaling pathway regulates the commitment and early development of T lymphocytes. We studied Notch-mediated induction of the pre-T cell receptor α (pTa) gene, a T-cell-specific...
Werner syndrome (WS) is an autosomal recessive disorder characterized by genomic instability and the premature onset of a number of age-related diseases. The gene responsible for WS encodes a member...
Pleiotropic defects in ataxia-telangiectasia protein-deficient mice
Elson, Ari, Wang, Yaoqi, Daugherty, Cathie J., Morton, Cynthia C., Zhou, Fen, Campos-Torres, Juanita, ...
We have generated a mouse model for ataxia-telangiectasia by using gene targeting to generate mice that do not express the Atm protein. Atm-deficient mice are retarded in growth, do not produce...
Wang, Y. Alan, Elson, Ari, Leder, Philip
Ataxia telangiectasia (AT) is an autosomal recessive disorder characterized by growth retardation, cerebellar ataxia, oculocutaneous telangiectasias, and a high incidence of lymphomas and leukemias....
A normal β-globin allele as a modifier gene ameliorating the severity of α-thalassemia in mice
Leder, Aya, Wiener, Edith, Lee, Matthew J., Wickramasinghe, Sunitha N., Leder, Philip
Thalassemia is a heritable human anemia caused by a variety of mutations that affect expression of the α- or the β-chain of hemoglobin. The expressivity of the phenotype is likely to be influenced...
Vollrath, Benedikt, Fitzgerald, Kevin J., Leder, Philip
The neurogenic gene brainiac was first isolated in Drosophila melanogaster, where it interacts genetically with members of the Notch signaling cascade. We have isolated a murine homologue of the...
Martin, Stuart S., Leder, Philip
Many tumor cells are impaired in adhesion-regulated apoptosis, which contributes to their metastatic potential. However, suppression of this apoptotic pathway in untransformed cells is not mediated...
Vollrath, Benedikt, Pudney, Jeffrey, Asa, Sylvia, Leder, Philip, Fitzgerald, Kevin
The Drosophila neuralized gene shows genetic interactions with Notch, Enhancer of split, and other neurogenic genes and is thought to be involved in cell fate specification in the central nervous...
Ecsedy, Jeffrey A., Michaelson, Jennifer S., Leder, Philip
We describe an interaction between homeodomain-interacting protein kinase 1 (HIPK1) and Daxx, two transcriptional regulators important in transducing growth-regulatory signals. We demonstrate that...
Critical Role for Mouse Hus1 in an S-Phase DNA Damage Cell Cycle Checkpoint
Weiss, Robert S., Leder, Philip, Vaziri, Cyrus
Mouse Hus1 encodes an evolutionarily conserved DNA damage response protein. In this study we examined how targeted deletion of Hus1 affects cell cycle checkpoint responses to genotoxic stress. Unlike...
Direct induction of T lymphocyte-specific gene expression by the mammalian Notch signaling pathway
The Notch signaling pathway regulates the commitment and early development of T lymphocytes. We studied Notch-mediated induction of the pre-T cell receptor α (pTa) gene, a T-cell-specific...
Trupin, Joel S., Rottman, Fritz M., Brimacombe, Richard L. C., Leder, Philip, Bernfield, Merton R., Nirenberg, Marshall W.
CELL-FREE PEPTIDE SYNTHESIS DEPENDENT UPON SYNTHETIC OLIGODEOXYNUCLEOTIDES*
Leder, Philip, Clark, Brian F. C., Sly, William S., Pestka, Sidney, Nirenberg, Marshall W.
TRANSLOCATION OF MRNA CODONS, II. PROPERTIES OF AN ANTI-TRANSLOCASE ANTIBODY
Leder, Philip, Skogerson, Lawrence E., Roufa, Donald J.
A purified preparation of translocase, one of several enzymes required for protein biosynthesis, has been used to prepare a specific anti-translocase antibody. This antibody provides an extremely...
TRANSLOCATION OF MRNA CODONS, I. THE PREPARATION AND CHARACTERISTICS OF A HOMOGENEOUS ENZYME
Leder, Philip, Skogerson, Lawrence E., Nau, Marion M.
This report describes a convenient scheme for the further purification of an E. coli enzyme which is required for the translocation step in protein biosynthesis. The homogeneous enzyme translocase...
Inactivation of mouse Hus1 results in genomic instability and impaired responses to genotoxic stress
Weiss, Robert S., Enoch, Tamar, Leder, Philip
The eukaryotic cell cycle is overseen by regulatory mechanisms, termed checkpoints, that respond to DNA damage, mitotic spindle defects, and errors in the ordering of cell cycle events. The DNA...
Michaelson, Jennifer S., Bader, Debra, Kuo, Frank, Kozak, Christine, Leder, Philip
The mammalian Daxx gene has been identified in a diverse set of yeast interaction trap experiments. Although a facilitating role for Daxx in Fas-induced apoptosis has been suggested, Daxx’s...
Chester, Nicholas, Kuo, Frank, Kozak, Christine, O’Hara, Cathie D., Leder, Philip
Bloom’s syndrome is a human autosomal genetic disorder characterized at the cellular level by genome instability and increased sister chomatid exchanges (SCEs). Clinical features of the disease...
An Association Between Globin Messenger RNA and 60S RNA Derived from Friend Leukemia Virus
Ikawa, Yoji, Ross, Jeffrey, Leder, Philip
The association between certain cellular RNAs and purified RNA tumor viruses prompted us to examine the possibility that specific host messenger RNAs might also be incorporated into RNA tumor...
Characterization of the Initial Peptide of Qβ RNA Polymerase and Control of Its Synthesis
Skogerson, Lawrence, Roufa, Donald, Leder, Philip
Bacteriophage Qβ RNA directs the cell-free synthesis of two fMet-containing dipeptides prior to the first round of ribosomal translocation. One of these, fMet-Ala, corresponds to the initial segment...
Globin Messenger-RNA Induction During Erythroid Differentiation of Cultured Leukemia Cells
Ross, Jeffrey, Ikawa, Yoji, Leder, Philip
A cloned line of murine proerythroblastoid cells (T-3-Cl-2), transformed by Friend leukemia virus, undergoes changes associated with erythroid differentiation when treated with dimethylsulfoxide in...
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to...
In Vitro Synthesis of DNA Complementary to Purified Rabbit Globin mRNA
Ross, Jeffrey, Aviv, Haim, Scolnick, Edward, Leder, Philip
Several properties of the viral RNA-dependent DNA polymerases and of rabbit globin mRNA make it possible to consider synthesis of the globin gene in vitro. These enzymes copy an RNA template using a...
The Organization and Diversity of Immunoglobulin Genes*
Leder, Philip, Honjo, Tasuku, Packman, Seymour, Swan, David, Nau, Marion, Norman, Barbara
We have used purified mouse immunoglobulin light chain mRNA and synthetic DNA which is complementary to it to assess the reiteration frequency of gene sequences corresponding to the κ constant...
Enzymatic Synthesis of Solid Phase-Bound DNA Sequences Corresponding to Specific Mammalian Genes
Venetianer, Pal, Leder, Philip
With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid...
Mutation of the Murine Bloom's Syndrome Gene Produces Global Genome Destabilization
Chester, Nicholas, Babbe, Holger, Pinkas, Jan, Manning, Charlene, Leder, Philip
Bloom's syndrome (BS) is a genetic disorder characterized cellularly by increases in sister chromatid exchanges (SCEs) and numbers of micronuclei. BS is caused by mutation in the BLM DNA helicase...
The Bloom's Syndrome Helicase Is Critical for Development and Function of the αβ T-Cell Lineage▿ †
Babbe, Holger, Chester, Nicholas, Leder, Philip, Reizis, Boris
Bloom's syndrome is a genetic disorder characterized by increased incidence of cancer and an immunodeficiency of unknown origin. The BLM gene mutated in Bloom's syndrome encodes a DNA helicase...
Lu, Jianrong, Ruhf, Marie-Laure, Perrimon, Norbert, Leder, Philip
Regulation of chromatin structure is critical in many fundamental cellular processes. Previous studies have suggested that the Rb tumor suppressor may recruit multiple chromatin regulatory proteins...
The expression of the pre-T cell receptor α (pTa) gene occurs exclusively in immature T lymphocytes and is regulated by poorly defined mechanisms. We have analyzed the role of the upstream enhancer...
The pre-T cell receptor α (pTα) protein is a critical component of the pre-T cell receptor complex in early thymocytes. The expression of the pTα gene is one of the earliest markers of the T cell...
Targeted Disruption of the Chemokine Eotaxin Partially Reduces Antigen-induced Tissue Eosinophilia
Rothenberg, Marc E., MacLean, James A., Pearlman, Eric, Luster, Andrew D., Leder, Philip
The chemokines are a large group of chemotactic cytokines that regulate leukocyte trafficking and have recently been shown to inhibit human immunodeficiency virus entry into cells. Eotaxin is a C–C...
Apoptosis is a genetically programmed series of events that results in cell death. As a consequence, it is difficult to identify dominant genes that play a role in this process using genetic...
Neu Differentiation Factor (NDF), a Dominant Oncogene, Causes Apoptosis In Vitro and In Vivo
Grimm, Stefan, Weinstein, Edward J., Krane, Ian M., Leder, Philip
Neu differentiation factor (NDF, also called neuregulin) is a potent inducer of epithelial cell proliferation and has been shown to induce mammary carcinomas in transgenic mice. Notwithstanding this...
A Network of Conserved Damage Survival Pathways Revealed by a Genomic RNAi Screen
Ravi, Dashnamoorthy, Wiles, Amy M., Bhavani, Selvaraj, Ruan, Jianhua, Leder, Philip, Bishop, Alexander J. R.
Damage initiates a pleiotropic cellular response aimed at cellular survival when appropriate. To identify genes required for damage survival, we used a cell-based RNAi screen against the Drosophila...