Baker, Diane R., Moxley, Rodney A., Steele, Mike B., LeJeune, Jeffrey T., Christopher-Hennings, Jane, Chen, Ding-Geng, ...
Escherichia coli O157:H7 causes life-threatening outbreaks of diarrhea, hemorrhagic colitis, and hemolyticuremic syndrome in humans and significant economic loss in agriculture and could be a...
Charge neutralization and DNA bending by the Escherichia coli catabolite activator protein (2002)
Hardwidge, Philip R., Zimmerman, Jeff M., Maher III, L. James
We are interested in the role of asymmetric phosphate neutralization in DNA bending induced by proteins. We describe an experimental estimate of the actual electrostatic contribution of asymmetric...
Experimental evaluation of the Liu-Beveridge dinucleotide step model of DNA structure (2001)
Hardwidge, Philip R., Maher, L. James
Methods for predicting DNA curvature have many possible applications. Dinucleotide step models describe DNA shape by characterization of helical twist, deflection angles and the direction of...
Design and calibration of a semi-synthetic DNA phasing assay (2000)
Hardwidge, Philip R., Zimmerman, Jeff M.
Electrophoretic assays of intrinsic DNA shape and shape changes induced by ligand binding are extremely useful because of their convenience and simplicity. The development of calibrations and...
Experimental evaluation of the Liu–Beveridge dinucleotide step model of DNA structure
Hardwidge, Philip R., Maher, L. James
Methods for predicting DNA curvature have many possible applications. Dinucleotide step models describe DNA shape by characterization of helical twist, deflection angles and the direction of...
HMG Proteins and DNA Flexibility in Transcription Activation
Ross, Eric D., Hardwidge, Philip R., Maher, L. James
The relative stiffness of naked DNA is evident from measured values of longitudinal persistence length (∼150 bp) and torsional persistence length (∼180 bp). These parameters predict that certain...
Charge neutralization and DNA bending by the Escherichia coli catabolite activator protein
Hardwidge, Philip R., Zimmerman, Jeff M., Maher, L. James
We are interested in the role of asymmetric phosphate neutralization in DNA bending induced by proteins. We describe an experimental estimate of the actual electrostatic contribution of asymmetric...
Design and calibration of a semi-synthetic DNA phasing assay
Hardwidge, Philip R., Zimmerman, Jeff M., Maher, L. James
Electrophoretic assays of intrinsic DNA shape and shape changes induced by ligand binding are extremely useful because of their convenience and simplicity. The development of calibrations and...
Hardwidge, Philip R., Deng, Wanyin, Vallance, Bruce A., Rodriguez-Escudero, Isabel, Cid, Victor J., Molina, Maria, ...
EspG is a conserved protein encoded by the locus of enterocyte effacement (LEE) of attaching and effacing (A/E) pathogens, including enteropathogenic and enterohemorrhagic Escherichia coli and...
Deng, Wanyin, Li, Yuling, Hardwidge, Philip R., Frey, Elizabeth A., Pfuetzner, Richard A., Lee, Sansan, ...
Human enteropathogenic Escherichia coli (EPEC), enterohemorrhagic E. coli (EHEC), and the mouse pathogen Citrobacter rodentium (CR) belong to the family of attaching and effacing (A/E) bacterial...
Dual Binding Modes for an HMG Domain from Human HMGB2 on DNA
McCauley, Micah, Hardwidge, Philip R., Maher, L. James, Williams, Mark C.
High mobility group B (HMGB) proteins contain two HMG box domains known to bind without sequence specificity into the DNA minor groove, slightly intercalating between basepairs and producing a strong...
Experimental evaluation of the Liu–Beveridge dinucleotide step model of DNA structure
Hardwidge, Philip R., Maher, L. James
Methods for predicting DNA curvature have many possible applications. Dinucleotide step models describe DNA shape by characterization of helical twist, deflection angles and the direction of...
HMG Proteins and DNA Flexibility in Transcription Activation
Ross, Eric D., Hardwidge, Philip R., Maher, L. James
The relative stiffness of naked DNA is evident from measured values of longitudinal persistence length (∼150 bp) and torsional persistence length (∼180 bp). These parameters predict that certain...
Charge neutralization and DNA bending by the Escherichia coli catabolite activator protein
Hardwidge, Philip R., Zimmerman, Jeff M., Maher, L. James
We are interested in the role of asymmetric phosphate neutralization in DNA bending induced by proteins. We describe an experimental estimate of the actual electrostatic contribution of asymmetric...
Design and calibration of a semi-synthetic DNA phasing assay
Hardwidge, Philip R., Zimmerman, Jeff M., Maher, L. James
Electrophoretic assays of intrinsic DNA shape and shape changes induced by ligand binding are extremely useful because of their convenience and simplicity. The development of calibrations and...
Hardwidge, Philip R., Deng, Wanyin, Vallance, Bruce A., Rodriguez-Escudero, Isabel, Cid, Victor J., Molina, Maria, ...
EspG is a conserved protein encoded by the locus of enterocyte effacement (LEE) of attaching and effacing (A/E) pathogens, including enteropathogenic and enterohemorrhagic Escherichia coli and...
Deng, Wanyin, Li, Yuling, Hardwidge, Philip R., Frey, Elizabeth A., Pfuetzner, Richard A., Lee, Sansan, ...
Human enteropathogenic Escherichia coli (EPEC), enterohemorrhagic E. coli (EHEC), and the mouse pathogen Citrobacter rodentium (CR) belong to the family of attaching and effacing (A/E) bacterial...
Dual Binding Modes for an HMG Domain from Human HMGB2 on DNA
McCauley, Micah, Hardwidge, Philip R., Maher, L. James, Williams, Mark C.
High mobility group B (HMGB) proteins contain two HMG box domains known to bind without sequence specificity into the DNA minor groove, slightly intercalating between basepairs and producing a strong...
Baker, Diane R., Moxley, Rodney A., Steele, Mike B., LeJeune, Jeffrey T., Christopher-Hennings, Jane, Chen, Ding-Geng, ...
Escherichia coli O157:H7 causes life-threatening outbreaks of diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome in humans and significant economic loss in agriculture and could be a...
Johnson, Amber M., Kaushik, Radhey S., Rotella, Nicholas J., Hardwidge, Philip R.
Enterotoxigenic Escherichia coli (ETEC) is a common cause of travelers' and postweaning diarrhea in humans and swine, respectively. The extent to which ETEC damages host cells is unclear. Experiments...
Heat-Labile Enterotoxin Promotes Escherichia coli Adherence to Intestinal Epithelial Cells▿
Johnson, Amber M., Kaushik, Radhey S., Francis, David H., Fleckenstein, James M., Hardwidge, Philip R.
Given recent evidence suggesting that the heat-labile enterotoxin (LT) provides a colonization advantage for enterotoxigenic Escherichia coli (ETEC) in vivo, we hypothesized that LT preconditions the...