Chen, Qiang, Fischer, Joshua Richard, Benoit, Vivian M., Dufour, Nicholas P., Youderian, Philip, Leong, John M.
Borrelia burgdorferi is the causative agent of Lyme disease, the most common vector-borne illness in the Northern hemisphere. Low-passage-number infectious strains of B. burgdorferi exhibit extremely...
High-Throughput Functional Analysis of the Synechococcus elongatus PCC 7942 Genome (2005)
Holtman, C. Kay, Chen, You, Sandoval, Pamela, Gonzales, Alejandra, Nalty, Mark S., Thomas, Terry L., ...
Synechococcus elongatus PCC 7942 was the first cyanobacterial strain to be reliably transformed by exogenously added DNA and has become the model organism for cyanobacterial circadian rhythms. With a...
The tryptophan repressor sequence is highly conserved among the Enterobacteriaceae (1994)
Arvidson, Dennis N., Grove Arvidson, Cindy, Lawson, Catherine L., Miner, Jami, Adams, Curtis, Youderian, Philip
Tryptophan biosynthesis in Escherichia coli is regulated by the product of the trpR gene, the tryptophan (Trp) repressor. Trp aporepressor binds the corepressor, L-tryptophan, to form a holorepressor...
Shapiro, Michael, Arvidson, Dennis N., Pfau, James, Youderian, Philip
The phenotypes of four mutant Escherichia coli Trp repressor proteins with increased activities have been examined in vivo using the challenge-phage assay, an assay based on a positive genetic...
Hughes, Kelly T., Youderian, Philip; Simon, Melvin I.
The bacteriophage P22-based challenge phase selection was used to characterize the binding of Salmonella Hin recombinase to the wild-type hixL and hixR recombination sites, as well as to mutant and...
Hughes, Kelly T., Youderian, Philip; Simon, Melvin I.
The bacteriophage P22-based challenge phase selection was used to characterize the binding of Salmonella Hin recombinase to the wild-type hixL and hixR recombination sites, as well as to mutant and...
Spontaneous mutations occur near dam recognition sites in a dam- Escherichia coli host (1987)
Carraway, Margaretha, Youderian, Philip, Marinus, Martin G.
The mismatch repair system of Escherichia coli K12 removes mispaired bases from DNA. Mismatch repair can occur on either strand of DNA if it lacks N6-methyladenines within 5'-GATC-3' sequences. In...
Youderian, Philip, Lawes, Matthew C., Creighton, Chad, Cook, Jessica C., Saier, Milton H.
The glucose analog 2-deoxyglucose (2dGlc) inhibits the growth and multicellular development of Myxococcus xanthus. Mutants of M. xanthus resistant to 2dGlc, designated hex mutants, arise at a low...
Magrini, Vincent, Creighton, Chad, Youderian, Philip
Like most temperate bacteriophages, phage Mx8 integrates into a preferred locus on the genome of its host, Myxococcus xanthus, by a mechanism of site-specific recombination. The Mx8 int-attP genes...
Magrini, Vincent, Storms, Michael L., Youderian, Philip
Temperate Myxococcus xanthus phage Mx8 integrates into the attB locus of the M. xanthus genome. The phage attachment site, attP, is required in cis for integration and lies within the int (integrase)...
Biocontrol of Escherichia coli O157 with O157-Specific Bacteriophages
Kudva, Indira T., Jelacic, Srdjan, Tarr, Phillip I., Youderian, Philip, Hovde, Carolyn J.
Escherichia coli O157 antigen-specific bacteriophages were isolated and tested to determine their ability to lyse laboratory cultures of Escherichia coli O157:H7. A total of 53 bovine or ovine fecal...
Genetic Determinants of Immunity and Integration of Temperate Myxococcus xanthus Phage Mx8
Salmi, Daniel, Magrini, Vincent, Hartzell, Patricia L., Youderian, Philip
An 8.1-kb fragment of the temperate Myxococcus xanthus phage Mx8 genome, when cloned into a plasmid vector, permits site-specific integration of the plasmid and confers superinfection immunity....
A Chaperone in the HSP70 Family Controls Production of Extracellular Fibrils in Myxococcus xanthus
Weimer, Robby M., Creighton, Chad, Stassinopoulos, Angela, Youderian, Philip, Hartzell, Patricia L.
Three independent Tn5-lac insertions in the S1 locus of Myxococcus xanthus inactivate the sglK gene, which is nonessential for growth but required for social motility and multicellular development....
Magrini, Vincent, Creighton, Chad, White, David, Hartzell, Patricia L., Youderian, Philip
Plasmids with the aadA gene from plasmid R100, which confers resistance to the aminoglycosides spectinomycin and streptomycin in Escherchia coli, can be introduced into wild-type Myxococcus xanthus,...
Global Regulation of the Salmonella enterica Serovar Typhimurium Major Porin, OmpD
Santiviago, Carlos A., Toro, Cecilia S., Hidalgo, Alejandro A., Youderian, Philip, Mora, Guido C.
The OmpD porin is the most abundant outer membrane protein in Salmonella enterica serovar Typhimurium and represents about 1% of total cell protein. Unlike the case with the less abundant OmpC and...
Tantaleán, Juan C., Araya, Manuel A., Saavedra, Claudia P., Fuentes, Derie E., Pérez, José M., Calderón, Iván L., ...
Many eubacteria are resistant to the toxic oxidizing agent potassium tellurite, and tellurite resistance involves diverse biochemical mechanisms. Expression of the iscS gene from Geobacillus...
Precise Excision of the Large Pathogenicity Island, SPI7, in Salmonella enterica Serovar Typhi
Bueno, Susan M., Santiviago, Carlos A., Murillo, Alejandro A., Fuentes, Juan A., Trombert, A. Nicole, Rodas, Paula I., ...
The large pathogenicity island (SPI7) of Salmonella enterica serovar Typhi is a 133,477-bp segment of DNA flanked by two 52-bp direct repeats overlapping the pheU (phenylalanyl-tRNA) gene, contains...
Bucarey, Sergio A., Villagra, Nicolás A., Martinic, Mara P., Trombert, A. Nicole, Santiviago, Carlos A., Maulén, Nancy P., ...
The Salmonella enterica serovar Typhi tsx gene encodes a porin that facilitates the import of nucleosides. When serovar Typhi is grown under anaerobic conditions, Tsx is among the outer membrane...
Mutations That Improve the ANT Promoter of Salmonella Phage P22
Graña, Dolores, Youderian, Philip, Susskind, Miriam M.
Mutations that increase the activity of the promoter for the antirepressor gene of phage P22 were isolated by pseudoreversion of four severe promoter-down mutations. The sequence changes in these...
General Selection for Specific DNA-Binding Activities
Benson, Nicholas, Sugiono, Paul, Bass, Steven, Mendelman, Lynn V., Youderian, Philip
We present a general strategy for the selection of bacterial clones that express DNA-binding activities corresponding to particular DNA recognition sites. The selection uses a "challenge phage"...
Spontaneous Mutations Occur near Dam Recognition Sites in a dam-Escherichia coli Host
Carraway, Margaretha, Youderian, Philip, Marinus, M. G.
The mismatch repair system of Escherichia coli K12 removes mispaired bases from DNA. Mismatch repair can occur on either strand of DNA if it lacks N6-methyladenines within 5'-GATC-3' sequences. In...
Transposon Insertions of magellan-4 That Impair Social Gliding Motility in Myxococcus xanthus
Youderian, Philip, Hartzell, Patricia L.
Myxococcus xanthus has two different mechanisms of motility, adventurous (A) motility, which permits individual cells to glide over solid surfaces, and social (S) motility, which permits groups of...
Insertions of mini-Tn10 transposon T-POP in Salmonella enterica sv. typhi.
Hidalgo, Alejandro A, Trombert, A Nicole, Castro-Alonso, J C, Santiviago, Carlos A, Tesser, Bruno R, Youderian, Philip, ...
We have mutagenized a clinical strain of Salmonella enterica sv. typhi with mini-transposon Tn10dTet (T-POP) to obtain conditional lethal (tetracycline-dependent) mutants with T-POP insertions...
Youderian, Philip, Lawes, Matthew C., Creighton, Chad, Cook, Jessica C., Saier, Milton H.
The glucose analog 2-deoxyglucose (2dGlc) inhibits the growth and multicellular development of Myxococcus xanthus. Mutants of M. xanthus resistant to 2dGlc, designated hex mutants, arise at a low...
Magrini, Vincent, Creighton, Chad, Youderian, Philip
Like most temperate bacteriophages, phage Mx8 integrates into a preferred locus on the genome of its host, Myxococcus xanthus, by a mechanism of site-specific recombination. The Mx8 int-attP genes...
Magrini, Vincent, Storms, Michael L., Youderian, Philip
Temperate Myxococcus xanthus phage Mx8 integrates into the attB locus of the M. xanthus genome. The phage attachment site, attP, is required in cis for integration and lies within the int (integrase)...
Biocontrol of Escherichia coli O157 with O157-Specific Bacteriophages
Kudva, Indira T., Jelacic, Srdjan, Tarr, Phillip I., Youderian, Philip, Hovde, Carolyn J.
Escherichia coli O157 antigen-specific bacteriophages were isolated and tested to determine their ability to lyse laboratory cultures of Escherichia coli O157:H7. A total of 53 bovine or ovine fecal...
Genetic Determinants of Immunity and Integration of Temperate Myxococcus xanthus Phage Mx8
Salmi, Daniel, Magrini, Vincent, Hartzell, Patricia L., Youderian, Philip
An 8.1-kb fragment of the temperate Myxococcus xanthus phage Mx8 genome, when cloned into a plasmid vector, permits site-specific integration of the plasmid and confers superinfection immunity....
A Chaperone in the HSP70 Family Controls Production of Extracellular Fibrils in Myxococcus xanthus
Weimer, Robby M., Creighton, Chad, Stassinopoulos, Angela, Youderian, Philip, Hartzell, Patricia L.
Three independent Tn5-lac insertions in the S1 locus of Myxococcus xanthus inactivate the sglK gene, which is nonessential for growth but required for social motility and multicellular development....
Magrini, Vincent, Creighton, Chad, White, David, Hartzell, Patricia L., Youderian, Philip
Plasmids with the aadA gene from plasmid R100, which confers resistance to the aminoglycosides spectinomycin and streptomycin in Escherchia coli, can be introduced into wild-type Myxococcus xanthus,...
Global Regulation of the Salmonella enterica Serovar Typhimurium Major Porin, OmpD
Santiviago, Carlos A., Toro, Cecilia S., Hidalgo, Alejandro A., Youderian, Philip, Mora, Guido C.
The OmpD porin is the most abundant outer membrane protein in Salmonella enterica serovar Typhimurium and represents about 1% of total cell protein. Unlike the case with the less abundant OmpC and...
Tantaleán, Juan C., Araya, Manuel A., Saavedra, Claudia P., Fuentes, Derie E., Pérez, José M., Calderón, Iván L., ...
Many eubacteria are resistant to the toxic oxidizing agent potassium tellurite, and tellurite resistance involves diverse biochemical mechanisms. Expression of the iscS gene from Geobacillus...
Precise Excision of the Large Pathogenicity Island, SPI7, in Salmonella enterica Serovar Typhi
Bueno, Susan M., Santiviago, Carlos A., Murillo, Alejandro A., Fuentes, Juan A., Trombert, A. Nicole, Rodas, Paula I., ...
The large pathogenicity island (SPI7) of Salmonella enterica serovar Typhi is a 133,477-bp segment of DNA flanked by two 52-bp direct repeats overlapping the pheU (phenylalanyl-tRNA) gene, contains...
Mutations That Improve the ANT Promoter of Salmonella Phage P22
Graña, Dolores, Youderian, Philip, Susskind, Miriam M.
Mutations that increase the activity of the promoter for the antirepressor gene of phage P22 were isolated by pseudoreversion of four severe promoter-down mutations. The sequence changes in these...
General Selection for Specific DNA-Binding Activities
Benson, Nicholas, Sugiono, Paul, Bass, Steven, Mendelman, Lynn V., Youderian, Philip
We present a general strategy for the selection of bacterial clones that express DNA-binding activities corresponding to particular DNA recognition sites. The selection uses a "challenge phage"...
Spontaneous Mutations Occur near Dam Recognition Sites in a dam-Escherichia coli Host
Carraway, Margaretha, Youderian, Philip, Marinus, M. G.
The mismatch repair system of Escherichia coli K12 removes mispaired bases from DNA. Mismatch repair can occur on either strand of DNA if it lacks N6-methyladenines within 5'-GATC-3' sequences. In...
Bucarey, Sergio A., Villagra, Nicolás A., Martinic, Mara P., Trombert, A. Nicole, Santiviago, Carlos A., Maulén, Nancy P., ...
The Salmonella enterica serovar Typhi tsx gene encodes a porin that facilitates the import of nucleosides. When serovar Typhi is grown under anaerobic conditions, Tsx is among the outer membrane...
Transposon Insertions of magellan-4 That Impair Social Gliding Motility in Myxococcus xanthus
Youderian, Philip, Hartzell, Patricia L.
Myxococcus xanthus has two different mechanisms of motility, adventurous (A) motility, which permits individual cells to glide over solid surfaces, and social (S) motility, which permits groups of...
Insertions of mini-Tn10 transposon T-POP in Salmonella enterica sv. typhi.
Hidalgo, Alejandro A, Trombert, A Nicole, Castro-Alonso, J C, Santiviago, Carlos A, Tesser, Bruno R, Youderian, Philip, ...
We have mutagenized a clinical strain of Salmonella enterica sv. typhi with mini-transposon Tn10dTet (T-POP) to obtain conditional lethal (tetracycline-dependent) mutants with T-POP insertions...
Triple Mutants Uncover Three New Genes Required for Social Motility in Myxococcus xanthus
Youderian, Philip, Hartzell, Patricia L.
The bacterium Myxococcus xanthus glides over surfaces using two different locomotive mechanisms, called S (social) and A (adventurous) motility that enable cells to move both as groups and as...
Chen, Qiang, Fischer, Joshua R., Benoit, Vivian M., Dufour, Nicholas P., Youderian, Philip, Leong, John M.
Borrelia burgdorferi is the causative agent of Lyme disease, the most common vector-borne illness in the Northern hemisphere. Low-passage-number infectious strains of B. burgdorferi exhibit extremely...