Pierre-Emmanuel Gleizes

Mutation of ribosomal protein RPS24 in Diamond-Blackfan anemia results in a ribosome biogenesis disorder (2008)

Choesmel, Valérie, Fribourg, Sébastien, Aguissa-Touré, Almass-Houd, Pinaud, Noël, Legrand, Pierre, Gazda, Hanna T., ...

Diamond-Blackfan anemia (DBA) is a rare congenital disease affecting erythroid precursor differentiation. DBA is emerging as a paradigm for a new class of pathologies potentially linked to disorders...

Molecular basis of Diamond Blackfan anemia: structure and function analysis of RPS19 (2007)

Gregory, Lynn A., Aguissa-Touré, Almass-Houd, Pinaud, Noël, Legrand, Pierre, Gleizes, Pierre-Emmanuel, Fribourg, Sébastien

Diamond–Blackfan anemia (DBA) is a rare congenital disease linked to mutations in the ribosomal protein genes rps19, rps24 and rps17. It belongs to the emerging class of ribosomal disorders. To...

Molecular basis of Diamond Blackfan anemia: structure and function analysis of RPS19. (2007)

Gregory, Lynn, Aguissa-Touré, Almass-Houd, Pinaud, Noël, Legrand, Pierre, Gleizes, Pierre-Emmanuel, Fribourg, Sébastien

Diamond-Blackfan anemia (DBA) is a rare congenital disease linked to mutations in the ribosomal protein genes rps19, rps24 and rps17. It belongs to the emerging class of ribosomal disorders. To...

Molecular basis of Diamond Blackfan anemia: structure and function analysis of RPS19. (2007)

Gregory, Lynn, Aguissa-Touré, Almass-Houd, Pinaud, Noël, Legrand, Pierre, Gleizes, Pierre-Emmanuel, Fribourg, Sébastien

Diamond-Blackfan anemia (DBA) is a rare congenital disease linked to mutations in the ribosomal protein genes rps19, rps24 and rps17. It belongs to the emerging class of ribosomal disorders. To...

Molecular basis of Diamond Blackfan anemia: structure and function analysis of RPS19. (2007)

Gregory, Lynn, Aguissa-Touré, Almass-Houd, Pinaud, Noël, Legrand, Pierre, Gleizes, Pierre-Emmanuel, Fribourg, Sébastien

Diamond-Blackfan anemia (DBA) is a rare congenital disease linked to mutations in the ribosomal protein genes rps19, rps24 and rps17. It belongs to the emerging class of ribosomal disorders. To...

Assembly and Functional Organization of the Nucleolus: Ultrastructural Analysis of Saccharomyces cerevisiae Mutants

Trumtel, Stéphanie, Léger-Silvestre, Isabelle, Gleizes, Pierre-Emmanuel, Teulières, Frédéric, Gas, Nicole

Using Saccharomyces cerevisiae strains with genetically modified nucleoli, we show here that changing parameters as critical as the tandem organization of the ribosomal genes and the polymerase...

Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps

Saveanu, Cosmin, Bienvenu, David, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Gas, Nicole, Jacquier, Alain, ...

Eukaryotic ribosome maturation depends on a set of well ordered processing steps. Here we describe the functional characterization of yeast Nog2p (Ynr053cp), a highly conserved nuclear protein. Nog2p...

Processing of 20S pre-rRNA to 18S ribosomal RNA in yeast requires Rrp10p, an essential non-ribosomal cytoplasmic protein

Vanrobays, Emmanuel, Gleizes, Pierre-Emmanuel, Bousquet-Antonelli, Cécile, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Gélugne, Jean-Paul

Numerous non-ribosomal trans-acting factors involved in pre-ribosomal RNA processing have been characterized, but none of them is specifically required for the last cytoplasmic steps of 18S rRNA...

Late Cytoplasmic Maturation of the Small Ribosomal Subunit Requires RIO Proteins in Saccharomyces cerevisiae

Vanrobays, Emmanuel, Gelugne, Jean-Paul, Gleizes, Pierre-Emmanuel, Caizergues-Ferrer, Michele

Numerous nonribosomal trans-acting factors involved in pre-rRNA processing have been characterized, but few of them are specifically required for the last cytoplasmic steps of 18S rRNA maturation. We...

Sequential Protein Association with Nascent 60S Ribosomal Particles

Saveanu, Cosmin, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Lebreton, Alice, Rousselle, Jean-Claude, Noaillac-Depeyre, Jacqueline, ...

Ribosome biogenesis in eukaryotes depends on the coordinated action of ribosomal and nonribosomal proteins that guide the assembly of preribosomal particles. These intermediate particles follow a...

The ribosomal protein Rps15p is required for nuclear exit of the 40S subunit precursors in yeast

Léger-Silvestre, Isabelle, Milkereit, Philipp, Ferreira-Cerca, Sébastien, Saveanu, Cosmin, Rousselle, Jean-Claude, Choesmel, Valérie, ...

We have conducted a genetic screen in order to identify ribosomal proteins of Saccharomyces cerevisiae involved in nuclear export of the small subunit precursors. This has led us to distinguish...

Nuclear export and cytoplasmic processing of precursors to the 40S ribosomal subunits in mammalian cells

Rouquette, Jacques, Choesmel, Valérie, Gleizes, Pierre-Emmanuel

It is generally assumed that, in mammalian cells, preribosomal RNAs are entirely processed before nuclear exit. Here, we show that pre-40S particles exported to the cytoplasm in HeLa cells contain...

Assembly and Functional Organization of the Nucleolus: Ultrastructural Analysis of Saccharomyces cerevisiae Mutants

Trumtel, Stéphanie, Léger-Silvestre, Isabelle, Gleizes, Pierre-Emmanuel, Teulières, Frédéric, Gas, Nicole

Using Saccharomyces cerevisiae strains with genetically modified nucleoli, we show here that changing parameters as critical as the tandem organization of the ribosomal genes and the polymerase...

Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps

Saveanu, Cosmin, Bienvenu, David, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Gas, Nicole, Jacquier, Alain, ...

Eukaryotic ribosome maturation depends on a set of well ordered processing steps. Here we describe the functional characterization of yeast Nog2p (Ynr053cp), a highly conserved nuclear protein. Nog2p...

Processing of 20S pre-rRNA to 18S ribosomal RNA in yeast requires Rrp10p, an essential non-ribosomal cytoplasmic protein

Vanrobays, Emmanuel, Gleizes, Pierre-Emmanuel, Bousquet-Antonelli, Cécile, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Gélugne, Jean-Paul

Numerous non-ribosomal trans-acting factors involved in pre-ribosomal RNA processing have been characterized, but none of them is specifically required for the last cytoplasmic steps of 18S rRNA...

Late Cytoplasmic Maturation of the Small Ribosomal Subunit Requires RIO Proteins in Saccharomyces cerevisiae

Vanrobays, Emmanuel, Gelugne, Jean-Paul, Gleizes, Pierre-Emmanuel, Caizergues-Ferrer, Michele

Numerous nonribosomal trans-acting factors involved in pre-rRNA processing have been characterized, but few of them are specifically required for the last cytoplasmic steps of 18S rRNA maturation. We...

Sequential Protein Association with Nascent 60S Ribosomal Particles

Saveanu, Cosmin, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Lebreton, Alice, Rousselle, Jean-Claude, Noaillac-Depeyre, Jacqueline, ...

Ribosome biogenesis in eukaryotes depends on the coordinated action of ribosomal and nonribosomal proteins that guide the assembly of preribosomal particles. These intermediate particles follow a...

The ribosomal protein Rps15p is required for nuclear exit of the 40S subunit precursors in yeast

Léger-Silvestre, Isabelle, Milkereit, Philipp, Ferreira-Cerca, Sébastien, Saveanu, Cosmin, Rousselle, Jean-Claude, Choesmel, Valérie, ...

We have conducted a genetic screen in order to identify ribosomal proteins of Saccharomyces cerevisiae involved in nuclear export of the small subunit precursors. This has led us to distinguish...

Nuclear export and cytoplasmic processing of precursors to the 40S ribosomal subunits in mammalian cells

Rouquette, Jacques, Choesmel, Valérie, Gleizes, Pierre-Emmanuel

It is generally assumed that, in mammalian cells, preribosomal RNAs are entirely processed before nuclear exit. Here, we show that pre-40S particles exported to the cytoplasm in HeLa cells contain...

Molecular basis of Diamond–Blackfan anemia: structure and function analysis of RPS19

Gregory, Lynn A., Aguissa-Touré, Almass-Houd, Pinaud, Noël, Legrand, Pierre, Gleizes, Pierre-Emmanuel, Fribourg, Sébastien

Diamond–Blackfan anemia (DBA) is a rare congenital disease linked to mutations in the ribosomal protein genes rps19, rps24 and rps17. It belongs to the emerging class of ribosomal disorders. To...

Impaired ribosome biogenesis in Diamond-Blackfan anemia

Choesmel, Valérie, Bacqueville, Daniel, Rouquette, Jacques, Noaillac-Depeyre, Jacqueline, Fribourg, Sébastien, Crétien, Aurore, ...

The gene encoding the ribosomal protein S19 (RPS19) is frequently mutated in Diamond-Blackfan anemia (DBA), a congenital erythroblastopenia. The consequence of these mutations on the onset of the...

Latent Transforming Growth Factor-β Binding Protein Domains Involved in Activation and Transglutaminase-dependent Cross-Linking of Latent Transforming Growth Factor-β

Nunes, Irene, Gleizes, Pierre-Emmanuel, Metz, Christine N., Rifkin, Daniel B

Transforming growth factor-β (TGF-β) is secreted by many cell types as part of a large latent complex composed of three subunits: TGF-β, the TGF-β propeptide, and the latent TGF-β binding...

Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex

Gleizes, Pierre-Emmanuel, Noaillac-Depeyre, Jacqueline, Léger-Silvestre, Isabelle, Teulières, Frédéric, Dauxois, Jean-Yves, Pommet, Denys, ...

To study the nuclear export of preribosomes, ribosomal RNAs were detected by in situ hybridization using fluorescence and EM, in the yeast Saccharomyces cerevisiae. In wild-type cells,...

Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processing

Gadal, Olivier, Strauss, Daniela, Petfalski, Elisabeth, Gleizes, Pierre-Emmanuel, Gas, Nicole, Tollervey, David, ...

Many analyses have examined subnucleolar structures in eukaryotic cells, but the relationship between morphological structures, pre-rRNA processing, and ribosomal particle assembly has remained...

Ribosomal Protein L5 and L11 Mutations Are Associated with Cleft Palate and Abnormal Thumbs in Diamond-Blackfan Anemia Patients

Gazda, Hanna T., Sheen, Mee Rie, Vlachos, Adrianna, Choesmel, Valerie, O'Donohue, Marie-Françoise, Schneider, Hal, ...

Diamond-Blackfan anemia (DBA), a congenital bone-marrow-failure syndrome, is characterized by red blood cell aplasia, macrocytic anemia, clinical heterogeneity, and increased risk of malignancy....