Pierre Legrain

French Roadmap for complex Systems 2008-2009 (2009)

Chavalarias, David, Bourgine, Paul, Perrier, Edith, Amblard, Fréderic, Arlabosse, François, Auger, Pierre, ...

A “complex system” is in general any system comprised of a great number of heterogeneous entities, among which local interactions create multiple levels of collective structure and organization....

French Roadmap for complex Systems 2008-2009 (2009)

Chavalarias, David, Bourgine, Paul, Perrier, Edith, Amblard, Fréderic, Arlabosse, François, Auger, Pierre, ...

A “complex system” is in general any system comprised of a great number of heterogeneous entities, among which local interactions create multiple levels of collective structure and organization....

InteroPORC: automated inference of highly conserved protein interaction networks (2008)

Michaut, Magali, Kerrien, Samuel, Montecchi-Palazzi, Luisa, Chauvat, Franck, Cassier-Chauvat, Corinne, Aude, Jean-Christophe, ...

Motivation: Protein–protein interaction networks provide insights into the relationships between the proteins of an organism thereby contributing to a better understanding of cellular processes....

Cadmium triggers an integrated reprogramming of the metabolism of SynechocystisPCC6803, under the control of the Slr1738 regulator (2007)

Houot, Laetitia, Floutier, Martin, Marteyn, Benoit, Michaut, Magali, Picciocchi, Antoine, Legrain, Pierre, ...

Abstract Background Cadmium is a persistent pollutant that threatens most biological organisms, including cyanobacteria that support a large part of the biosphere. Using a multifaceted approach, we...

ARHGAP10 is necessary for alpha-catenin recruitment at adherens junctions and for Listeria invasion. (2005)

Sousa, Sandra, Cabanes, Didier, Archambaud, Cristel, Colland, Frédéric, Lemichez, Emmanuel, Popoff, Michel, ...

E-cadherin mediates the formation of adherens junctions between epithelial cells. It serves as a receptor for Listeria monocytogenes, a bacterial pathogen that enters epithelial cells. The L....

Myosin XVa and whirlin, two deafness gene products required for hair bundle growth, are located at the stereocilia tips and interact directly (2005)

Delprat, Benjamin, Michel, Vincent, Goodyear, Richard, Yamasaki, Yasuhiro, Michalski, Nicolas, El-Amraoui, Aziz, ...

Defects in myosin XVa and the PDZ domain-containing protein, whirlin, underlie deafness in humans and mice. Hair bundles of mutant mice defective for either protein have abnormally short stereocilia....

Protein interaction mapping: A Drosophila case study (2005)

Formstecher, Etienne, Aresta, Sandra, Collura, Vincent, Hamburger, Alexandre, Meil, Alain, Trehin, Alexandra, ...

The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based...

Protein interaction mapping: A Drosophila case study (2005)

Formstecher, Etienne, Aresta, Sandra, Collura, Vincent, Hamburger, Alexandre, Meil, Alain, Trehin, Alexandra, ...

The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based...

Myosin XVa and whirlin, two deafness gene products required for hair bundle growth, are located at the stereocilia tips and interact directly (2004)

Delprat, Benjamin, Michel, Vincent, Goodyear, Richard, Yamasaki, Yasuhiro, Michalski, Nicolas, El-Amraoui, Aziz, ...

Defects in myosin XVa and the PDZ domain-containing protein, whirlin, underlie deafness in humans and mice. Hair bundles of mutant mice defective for either protein have abnormally short stereocilia....

Functional Proteomics Mapping of a Human Signaling Pathway (2004)

Colland, Frédéric, Jacq, Xavier, Trouplin, Virginie, Mougin, Christelle, Groizeleau, Caroline, Hamburger, Alexandre, ...

Access to the human genome facilitates extensive functional proteomics studies. Here, we present an integrated approach combining large-scale protein interaction mapping, exploration of the...

Myosin XVa and whirlin, two deafness gene products required for hair bundle growth, are located at the stereocilia tips and interact directly (2004)

Delprat, Benjamin, Michel, Vincent, Goodyear, Richard, Yamasaki, Yasuhiro, Michalski, Nicolas, El-Amraoui, Aziz, ...

Defects in myosin XVa and the PDZ domain-containing protein, whirlin, underlie deafness in humans and mice. Hair bundles of mutant mice defective for either protein have abnormally short stereocilia....

Genome-Wide Protein Interaction Screens Reveal Functional Networks Involving Sm-Like Proteins (2000)

Micheline Fromont-Racine, Andrew E. Mayes, Adeline Brunet-Simon, Jean-Christophe Rain, Alan Colley, Ian Dix, ...

A set of seven structurally related Sm proteins forms the core of the snRNP particles containing the spliceosomal U1, U2, U4 and U5 snRNAs. A search of the genomic sequence of Saccharomyces...

Homodimerization of the human U1 snRNP-specific protein C (1995)

Van Aarssen, Yvonne, Wassenaar, Roel, Legrain, Pierre, Van Venrooij, Walther J., Nelissen, Rob L.H.

The U1 snRNP-specific protein C contains an N-terminal zinc finger-like CH motif which Is required for the binding of the U1C protein to the U1 snRNP particle. Recently a similar motif was reported...

Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo (1994)

Kramer, Angela, Legrain, Pierre, Mulhauser, Frank, Groöining, Karsten, Brosi, Reto, Bilbe, Graeme

A cDNA encoding the 60 kDa subunit of mammalian splicing factor SF3a has been isolated. The deduced protein sequence reveals a 30% Identity to the PRP9 splicing protein of the yeast S.cerevisiae. The...

Interaction between the first and last nucleotides of pre-mRNA introns is a determinant of 3' splice site selection in S.cerevisiae (1994)

Chanfreau, Guillaume, Legrain, Pierre, Dujon, Bernard, Jacquier, Alain

The splicing of group II and nuclear pre-mRNAs introns occurs via a similar splicing pathway and some of the RNA-RNA interactions involved in these splicing reactions show structural similarities....

The biochemical defects of prp4-1 and prp6-1 yeast splicing mutants reveal that the PRP6 protein is required for the accumulation of the [U4/U6.U5] tri-snRNP (1993)

Galisson, Frederique, Legrain, Pierre

We have raised specific antibodies against the PRP6 protein and shown that the U4, U5 and U6 snRNAs are co-precipitated with this protein. Using splicing extracts prepared from In vivo...

Parallel analysis of genetic selections using whole genome oligonucleotide arrays

Cho, Raymond J., Fromont-Racine, Micheline, Wodicka, Lisa, Feierbach, Becket, Stearns, Timothy, Legrain, Pierre, ...

Thousands of genes have recently been sequenced in organisms ranging from Escherichia coli to human. For the majority of these genes, however, available sequence does not define a biological role....

Identification of Novel Saccharomyces cerevisiae Proteins with Nuclear Export Activity: Cell Cycle-Regulated Transcription Factor Ace2p Shows Cell Cycle-Independent Nucleocytoplasmic Shuttling

Jensen, Torben Heick, Neville, Megan, Rain, Jean Christophe, McCarthy, Terri, Legrain, Pierre, Rosbash, Michael

Nuclear export of proteins containing leucine-rich nuclear export signals (NESs) is mediated by the NES receptor CRM1/Crm1p. We have carried out a yeast two-hybrid screen with Crm1p as a bait. The...

Functional Conservation of the Transportin Nuclear Import Pathway in Divergent Organisms

Siomi, Mikiko C., Fromont, Micheline, Rain, Jean-Christophe, Wan, Lili, Wang, Fan, Legrain, Pierre, ...

Human transportin1 (hTRN1) is the nuclear import receptor for a group of pre-mRNA/mRNA-binding proteins (heterogeneous nuclear ribonucleoproteins [hnRNP]) represented by hnRNP A1, which shuttle...

Myosin VIIa, harmonin and cadherin 23, three Usher I gene products that cooperate to shape the sensory hair cell bundle

Boëda, Batiste, El-Amraoui, Aziz, Bahloul, Amel, Goodyear, Richard, Daviet, Laurent, Blanchard, Stéphane, ...

Deaf-blindness in three distinct genetic forms of Usher type I syndrome (USH1) is caused by defects in myosin VIIa, harmonin and cadherin 23. Despite being critical for hearing, the functions of...

Antiviral Drug Discovery Strategy Using Combinatorial Libraries of Structurally Constrained Peptides

Real, Eléonore, Rain, Jean-Christophe, Battaglia, Véronique, Jallet, Corinne, Perrin, Pierre, Tordo, Noël, ...

We have developed a new strategy for antiviral peptide discovery by using lyssaviruses (rabies virus and rabies-related viruses) as models. Based on the mimicry of natural bioactive peptides, two...

Functional Proteomics Mapping of a Human Signaling Pathway

Colland, Frédéric, Jacq, Xavier, Trouplin, Virginie, Mougin, Christelle, Groizeleau, Caroline, Hamburger, Alexandre, ...

Access to the human genome facilitates extensive functional proteomics studies. Here, we present an integrated approach combining large-scale protein interaction mapping, exploration of the...

The Shigella flexneri effector OspG interferes with innate immune responses by targeting ubiquitin-conjugating enzymes

Kim, Dong Wook, Lenzen, Gerlinde, Page, Anne-Laure, Legrain, Pierre, Sansonetti, Philippe J., Parsot, Claude

Bacteria of Shigella spp. are responsible for shigellosis in humans. They use a type III secretion system to inject effector proteins into host cells and induce their entry into epithelial cells or...

Protein interaction mapping: A Drosophila case study

Formstecher, Etienne, Aresta, Sandra, Collura, Vincent, Hamburger, Alexandre, Meil, Alain, Trehin, Alexandra, ...

The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based...

Parallel analysis of genetic selections using whole genome oligonucleotide arrays

Cho, Raymond J., Fromont-Racine, Micheline, Wodicka, Lisa, Feierbach, Becket, Stearns, Timothy, Legrain, Pierre, ...

Thousands of genes have recently been sequenced in organisms ranging from Escherichia coli to human. For the majority of these genes, however, available sequence does not define a biological role....

Identification of Novel Saccharomyces cerevisiae Proteins with Nuclear Export Activity: Cell Cycle-Regulated Transcription Factor Ace2p Shows Cell Cycle-Independent Nucleocytoplasmic Shuttling

Jensen, Torben Heick, Neville, Megan, Rain, Jean Christophe, McCarthy, Terri, Legrain, Pierre, Rosbash, Michael

Nuclear export of proteins containing leucine-rich nuclear export signals (NESs) is mediated by the NES receptor CRM1/Crm1p. We have carried out a yeast two-hybrid screen with Crm1p as a bait. The...

Functional Conservation of the Transportin Nuclear Import Pathway in Divergent Organisms

Siomi, Mikiko C., Fromont, Micheline, Rain, Jean-Christophe, Wan, Lili, Wang, Fan, Legrain, Pierre, ...

Human transportin1 (hTRN1) is the nuclear import receptor for a group of pre-mRNA/mRNA-binding proteins (heterogeneous nuclear ribonucleoproteins [hnRNP]) represented by hnRNP A1, which shuttle...

Myosin VIIa, harmonin and cadherin 23, three Usher I gene products that cooperate to shape the sensory hair cell bundle

Boëda, Batiste, El-Amraoui, Aziz, Bahloul, Amel, Goodyear, Richard, Daviet, Laurent, Blanchard, Stéphane, ...

Deaf-blindness in three distinct genetic forms of Usher type I syndrome (USH1) is caused by defects in myosin VIIa, harmonin and cadherin 23. Despite being critical for hearing, the functions of...

Antiviral Drug Discovery Strategy Using Combinatorial Libraries of Structurally Constrained Peptides

Real, Eléonore, Rain, Jean-Christophe, Battaglia, Véronique, Jallet, Corinne, Perrin, Pierre, Tordo, Noël, ...

We have developed a new strategy for antiviral peptide discovery by using lyssaviruses (rabies virus and rabies-related viruses) as models. Based on the mimicry of natural bioactive peptides, two...

Functional Proteomics Mapping of a Human Signaling Pathway

Colland, Frédéric, Jacq, Xavier, Trouplin, Virginie, Mougin, Christelle, Groizeleau, Caroline, Hamburger, Alexandre, ...

Access to the human genome facilitates extensive functional proteomics studies. Here, we present an integrated approach combining large-scale protein interaction mapping, exploration of the...

Protein interaction mapping: A Drosophila case study

Formstecher, Etienne, Aresta, Sandra, Collura, Vincent, Hamburger, Alexandre, Meil, Alain, Trehin, Alexandra, ...

The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based...

The Shigella flexneri effector OspG interferes with innate immune responses by targeting ubiquitin-conjugating enzymes

Kim, Dong Wook, Lenzen, Gerlinde, Page, Anne-Laure, Legrain, Pierre, Sansonetti, Philippe J., Parsot, Claude

Bacteria of Shigella spp. are responsible for shigellosis in humans. They use a type III secretion system to inject effector proteins into host cells and induce their entry into epithelial cells or...

Genome-Wide Protein Interaction Screens Reveal Functional Networks Involving Sm-Like Proteins

Fromont-Racine, Micheline, Mayes, Andrew E., Brunet-Simon, Adeline, Rain, Jean-Christophe, Colley, Alan, Dix, Ian, ...

A set of seven structurally related Sm proteins forms the core of the snRNP particles containing the spliceosomal U1, U2, U4 and U5 snRNAs. A search of the genomic sequence of Saccharomyces...