Pierre Rouzé

Open access to sequence: Browsing the Pichia pastorisgenome (2009)

Mattanovich, Diethard, Callewaert, Nico, Rouzé, Pierre, Lin, Yao-Cheng, Graf, Alexandra, Redl, Andreas, ...

Abstract The first genome sequences of the important yeast protein production host Pichia pastoris have been released into the public domain this spring. In order to provide the scientific community...

Predicting protein-protein interactions in Arabidopsis thalianathrough integration of orthology, gene ontology and co-expression (2009)

De Bodt, Stefanie, Proost, Sebastian, Vandepoele, Klaas, Rouzé, Pierre, Van De Peer, Yves

Abstract Background Large-scale identification of the interrelationships between different components of the cell, such as the interactions between proteins, has recently gained great interest....

Global expression analysis of the brown alga Ectocarpus siliculosus(Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress (2009)

Dittami, Simon M, Scornet, Delphine, Petit, Jean-Louis, Ségurens, Béatrice, Da Silva, Corinne, Corre, Erwan, ...

Abstract Background Brown algae (Phaeophyceae) are phylogenetically distant from red and green algae and an important component of the coastal ecosystem. They have developed unique mechanisms that...

Green Evolution and Dynamic Adaptations Revealed by Genomes of the Marine Picoeukaryotes Micromonas (2009)

Worden, Alexandra Z., Lee, Jea-Hyeok, Mock, Thomas, Rouzé, Pierre, Simmons, Melinda P., Aerts, Andrea L., ...

Picoeukaryotes are a taxonomically diverse group of organisms less than 2 micrometers in diameter. Photosynthetic marine picoeukaryotes in the genus Micromonas thrive in ecosystems ranging from...

Genome sequence of the recombinant protein production host Pichia pastoris (2009)

De Schutter, Kristof, Lin, Yao-Cheng, Tiels, Petra, Van Hecke, Annelies, Glinka, S, Weber-Lehmann, J, ...

The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as a model organism for studying peroxisomal biogenesis and methanol assimilation. P. pastoris strains...

Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress (2009)

Dittami, Simon, Scornet, Delphine, Petit, Jean-Louis, Ségurens, Béatrice, Da Silva, Corinne, Corre, Erwan, ...

Background: Brown algae (Phaeophyceae) are phylogenetically distant from red and green algae and an important component of the coastal ecosystem. They have developed unique mechanisms that allow them...

Predicting protein-protein interactions in Arabidopsis thaliana through integration of orthology, gene ontology and co-expression (2009)

De Bodt, Stefanie, Proost, Sebastian, Vandepoele, Klaas, Rouzé, Pierre, Van De Peer, Yves

Background: Large-scale identification of the interrelationships between different components of the cell, such as the interactions between proteins, has recently gained great interest. However,...

DOI: 10.1093/nar/gkg071 CATMA: a complete Arabidopsis GST database (2008)

Mark L. Crowe, Carine Serizet, Vincent Thareau, Sébastien Aubourg, Pierre Rouzé, Pierre Hilson, ...

The Complete Arabidopsis Transcriptome Micro Array (CATMA) database contains gene sequence tag (GST) and gene model sequences for over 70% of the predicted genes in the Arabidopsis thaliana genome as...

Original paper In search of the small ones: improved prediction of short exons in vertebrates, plants, fungi, and protists (2008)

Yvan Saeys, Pierre Rouzé, Alfonso Valencia

Motivation: Prediction of the coding potential for stretches of DNA is crucial in gene calling and genome annotation, where it is used to identify potential exons and to position their boundaries in...

Generic eukaryotic core promoter prediction using structural features of DNA (2008)

Abeel, Thomas, Saeys, Yvan, Bonnet, Eric, Rouzé, Pierre, Van De Peer, Yves

Despite many recent efforts, in silico identification of promoter regions is still in its infancy. However, the accurate identification and delineation of promoter regions is important for several...

ProSOM: core promoter prediction based on unsupervised clustering of DNA physical profiles (2008)

Abeel, Thomas, Saeys, Yvan, Rouzé, Pierre, Van De Peer, Yves

Motivation: More and more genomes are being sequenced, and to keep up with the pace of sequencing projects, automated annotation techniques are required. One of the most challenging problems in...

Gene finding integrating external evidence (2007)

Thomas Schiex, Catherine Mathé, Annick Moisan, Pierre Rouzé

EuGène, a gene finder for Arabidopsis thaliana, combines the output of several information sources, including output of other software or user information. To achieve this, a weighted DAG is built...

In search of the small ones: improved prediction of short exons in vertebrates, plants, fungi and protists (2007)

Saeys, Yvan, Rouzé, Pierre, Van De Peer, Yves

Motivation: Prediction of the coding potential for stretches of DNA is crucial in gene calling and genome annotation, where it is used to identify potential exons and to position their boundaries in...

In search of the small ones: improved prediction of short exons in vertebrates, plants, fungi, and protists (2007)

Saeys, Yvan, Rouzé, Pierre, Van De Peer, Yves

Motivation: Prediction of the coding potential for stretches of DNA is crucial in gene calling and genome annotation, where it is used to identify potential exons and to position their boundaries in...

in (2005)

Kobe Florquin, Yvan Saeys, Sven Degroeve, Pierre Rouzé

Large-scale structural analysis of the core promoter

GeneFarm, structural and functional annotation of Arabidopsis gene and protein families by a network of experts (2005)

Aubourg, Sébastien, Brunaud, Véronique, Bruyère, Clémence, Cock, Mark, Cooke, Richard, Cottet, Annick, ...

Genomic projects heavily depend on genome annotations and are limited by the current deficiencies in the published predictions of gene structure and function. It follows that, improved annotation...

Large-scale structural analysis of the core promoter in mammalian and plant genomes (2005)

Florquin, Kobe, Saeys, Yvan, Degroeve, Sven, Rouzé, Pierre, Van De Peer, Yves

DNA encodes at least two independent levels of functional information. The first level is for encoding proteins and sequence targets for DNA-binding factors, while the second one is contained in the...

SpliceMachine: predicting splice sites from high-dimensional local context representations (2005)

Degroeve, Sven, Saeys, Yvan, De Baets, Bernard, Rouzé, Pierre, Van De Peer, Yves

Motivation: In this age of complete genome sequencing, finding the location and structure of genes is crucial for further molecular research. The accurate prediction of intron boundaries largely...

Feature selection for splice site prediction: A new method using EDA-based feature ranking (2004)

Saeys, Yvan, Degroeve, Sven, Aeyels, Dirk, Rouzé, Pierre, Van De Peer, Yves

Abstract Background The identification of relevant biological features in large and complex datasets is an important step towards gaining insight in the processes underlying the data. Other...

BMC Bioinformatics Methodology article Feature selection for splice site prediction: A new method using EDA-based feature ranking (2004)

Yvan Saeys, Sven Degroeve, Dirk Aeyels, Pierre Rouzé, Yves Van De, Biomed Central

Background: The identification of relevant biological features in large and complex datasets is an important step towards gaining insight in the processes underlying the data. Other advantages of...

Evidence that microRNA precursors, unlike other non-coding RNAs, have lower folding free energies than random sequences (2004)

Bonnet, Eric, Wuyts, Jan, Rouzé, Pierre, Van De Peer, Yves

Motivation: Most non-coding RNAs are characterized by a specific secondary and tertiary structure that determines their function. Here, we investigate the folding energy of the secondary structure of...

SpliceMachine: predicting splice sites from high-dimensional local context representations (2004)

Degroeve, Sven, Saeys, Yvan, De Baets, Bernard, Rouzé, Pierre, Van De Peer, Yves

Motivation: In this age of complete genome sequencing, finding the location and structure of genes is crucial for further molecular research. The accurate prediction of intron boundaries largely...

Evidence that microRNA precursors, unlike other non-coding RNAs, have lower folding free energies than random sequences (2004)

Bonnet, Eric, Wuyts, Jan, Rouzé, Pierre, Van De Peer, Yves

Motivation: Most non-coding RNAs are characterized by a specific secondary and tertiary structure that determines their function. Here, we investigate the folding energy of the secondary structure of...

Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications (2004)

Hilson, Pierre, Allemeersch, Joke, Altmann, Thomas, Aubourg, Sébastien, Avon, Alexandra, Beynon, Jim, ...

Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization...

Evidence that microRNA precursors, unlike other non-coding RNAs, have lower folding free energies than random sequences (2004)

Bonnet, Eric, Wuyts, Jan, Rouzé, Pierre, Van De Peer, Yves

Motivation: Most non-coding RNAs are characterized by a specific secondary and tertiary structure that determines their function. Here, we investigate the folding energy of the secondary structure of...

SpliceMachine: predicting splice sites from high-dimensional local context representations (2004)

Degroeve, Sven, Saeys, Yvan, De Baets, Bernard, Rouzé, Pierre, Van De Peer, Yves

Motivation: In this age of complete genome sequencing, finding the location and structure of genes is crucial for further molecular research. The accurate prediction of intron boundaries largely...

CATMA: a complete Arabidopsis GST database (2003)

Crowe, Mark L., Serizet, Carine, Thareau, Vincent, Aubourg, Sébastien, Rouzé, Pierre, Hilson, Pierre, ...

The Complete Arabidopsis Transcriptome Micro Array (CATMA) database contains gene sequence tag (GST) and gene model sequences for over 70% of the predicted genes in the Arabidopsis thaliana...

Fast feature selection using a simple estimation of distribution algorithm: a case study on splice site prediction (2003)

Saeys, Yvan, Degroeve, Sven, Aeyels, Dirk, Van De Peer, Yves, Rouzé, Pierre

Motivation: Feature subset selection is an important preprocessing step for classification. In biology, where structures or processes are described by a large number of features, the elimination of...

Automatic design of gene-specific sequence tags for genome-wide functional studies (2003)

Thareau, Vincent, Déhais, Patrice, Serizet, Carine, Hilson, Pierre, Rouzé, Pierre, Aubourg, Sébastien

Motivation: The availability of complete genome sequences allows the identification of short DNA segments that are specific to each annotated gene. Such unique gene sequence tags (GSTs) replace...

CATMA: a complete Arabidopsis GST database (2002)

Crowe,Mark L., Serizet,Carine, Thareau,Vincent, Aubourg,Sébastien, Rouzé,Pierre, Hilson,Pierre, ...

The Complete Arabidopsis Transcriptome Micro Array (CATMA) database contains gene sequence tag (GST) and gene model sequences for over 70% of the predicted genes in the Arabidopsis thaliana genome as...

CATMA: a complete Arabidopsis GST database (2002)

Crowe, Mark L., Serizet, Carine, Thareau, Vincent, Aubourg, Sébastien, Rouzé, Pierre, Hilson, Pierre, ...

The Complete Arabidopsis Transcriptome Micro Array (CATMA) database contains gene sequence tag (GST) and gene model sequences for over 70% of the predicted genes in the Arabidopsis thaliana genome as...

A Gibbs sampling method to detect overrepresented motifs in the upstream regions of coexpressed genes (2002)

Gert Thijs, Kathleen Marchal, Magali Lescot, Stephane Rombauts, Bart De Moor, Pierre Rouzé, ...

Microarray experiments can reveal important information about transcriptional regulation. In our case, we look for potential promoter regulatory elements in the upstream region of coexpressed genes....

Pages S75–S83 Feature subset selection for splice site prediction (2002)

Sven Degroeve, Bernard De Baets, Pierre Rouzé

Motivation: The large amount of available annotated Arabidopsis thaliana sequences allows the induction of splice site prediction models with supervised learning algorithms (see Haussler (1998) for a...

PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences (2002)

Lescot, Magali, Déhais, Patrice, Thijs, Gert, Marchal, Kathleen, Moreau, Yves, Van De Peer, Yves, ...

PlantCARE is a database of plant cis-acting regulatory elements, enhancers and repressors. Regulatory elements are represented by positional matrices, consensus sequences and individual sites on...

Feature subset selection for splice site prediction (2002)

Degroeve, Sven, De Baets, Bernard, Van De Peer, Yves, Rouzé, Pierre

Motivation: The large amount of available annotated Arabidopsis thaliana sequences allows the induction of splice site prediction models with supervised learning algorithms (see Haussler (1998) for a...

Current methods of gene prediction, their strengths and weaknesses (2002)

Mathé, Catherine, Sagot, Marie-France, Schiex, Thomas, Rouzé, Pierre

While the genomes of many organisms have been sequenced over the last few years, transforming such raw sequence data into knowledge remains a hard task. A great number of prediction programs have...

A higher-order background model improves the detection of promoter regulatory elements by Gibbs sampling (2001)

Thijs, Gert, Lescot, Magali, Marchal, Kathleen, Rombauts, Stephane, De Moor, Bart, Rouzé, Pierre, ...

Motivation: Transcriptome analysis allows detection and clustering of genes that are coexpressed under various biological circumstances. Under the assumption that coregulated genes share cis-acting...

Evaluation of gene prediction software using a genomic data set: application to Arabidopsis thalianasequences (1999)

Pavy, Nathalie, Rombauts, Stephane, Déhais, Patrice, Mathé, Catherine, V. V.Ramana, Davuluri, Leroy, Philippe, ...

Motivation: The annotation of the Arabidopsis thalianagenome remains a problem in terms of time and quality. To improve the annotation process, we want to choose the most appropriate tools to use...

Splice site prediction in Arabidopsis thaliana pr-emRNA by combining local and global sequence information (1996)

Stefan M. Hebsgaard, Peter G. Korning, Niels Tolstrup, Jacob Engelbrecht, Pierre Rouzé, Søren Brunak

Artificial neural networks have been combined with a rule based system to predict intron splice sites in the dicot plant Arabidopsis thaliana. A two step prediction scheme, where a global prediction...

PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences

Lescot, Magali, Déhais, Patrice, Thijs, Gert, Marchal, Kathleen, Moreau, Yves, Van De Peer, Yves, ...

PlantCARE is a database of plant cis-acting regulatory elements, enhancers and repressors. Regulatory elements are represented by positional matrices, consensus sequences and individual sites on...

SURVEY AND SUMMARY: Current methods of gene prediction, their strengths and weaknesses

Mathé, Catherine, Sagot, Marie-France, Schiex, Thomas, Rouzé, Pierre

While the genomes of many organisms have been sequenced over the last few years, transforming such raw sequence data into knowledge remains a hard task. A great number of prediction programs have...

Genome-Wide Analysis of Core Cell Cycle Genes in Arabidopsis

Vandepoele, Klaas, Raes, Jeroen, De Veylder, Lieven, Rouzé, Pierre, Rombauts, Stephane, Inzé, Dirk

Cyclin-dependent kinases and cyclins regulate with the help of different interacting proteins the progression through the eukaryotic cell cycle. A high-quality, homology-based annotation protocol was...

CATMA: a complete Arabidopsis GST database

Crowe, Mark L., Serizet, Carine, Thareau, Vincent, Aubourg, Sébastien, Rouzé, Pierre, Hilson, Pierre, ...

The Complete Arabidopsis Transcriptome Micro Array (CATMA) database contains gene sequence tag (GST) and gene model sequences for over 70% of the predicted genes in the Arabidopsis thaliana genome as...

Computational Approaches to Identify Promoters and cis-Regulatory Elements in Plant Genomes1

Rombauts, Stephane, Florquin, Kobe, Lescot, Magali, Marchal, Kathleen, Rouzé, Pierre, Van De Peer, Yves

The identification of promoters and their regulatory elements is one of the major challenges in bioinformatics and integrates comparative, structural, and functional genomics. Many different...

Detection of 91 potential conserved plant microRNAs in Arabidopsis thaliana and Oryza sativa identifies important target genes

Bonnet, Eric, Wuyts, Jan, Rouzé, Pierre, Van De Peer, Yves

MicroRNAs (miRNAs) are an extensive class of tiny RNA molecules that regulate the expression of target genes by means of complementary base pair interactions. Although the first miRNAs were...

Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications

Hilson, Pierre, Allemeersch, Joke, Altmann, Thomas, Aubourg, Sébastien, Avon, Alexandra, Beynon, Jim, ...

Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization...

GeneFarm, structural and functional annotation of Arabidopsis gene and protein families by a network of experts

Aubourg, Sébastien, Brunaud, Véronique, Bruyère, Clémence, Cock, Mark, Cooke, Richard, Cottet, Annick, ...

Genomic projects heavily depend on genome annotations and are limited by the current deficiencies in the published predictions of gene structure and function. It follows that, improved annotation...

Large-scale structural analysis of the core promoter in mammalian and plant genomes

Florquin, Kobe, Saeys, Yvan, Degroeve, Sven, Rouzé, Pierre, Van De Peer, Yves

DNA encodes at least two independent levels of functional information. The first level is for encoding proteins and sequence targets for DNA-binding factors, while the second one is contained in the...

PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences

Lescot, Magali, Déhais, Patrice, Thijs, Gert, Marchal, Kathleen, Moreau, Yves, Van De Peer, Yves, ...

PlantCARE is a database of plant cis-acting regulatory elements, enhancers and repressors. Regulatory elements are represented by positional matrices, consensus sequences and individual sites on...

SURVEY AND SUMMARY: Current methods of gene prediction, their strengths and weaknesses

Mathé, Catherine, Sagot, Marie-France, Schiex, Thomas, Rouzé, Pierre

While the genomes of many organisms have been sequenced over the last few years, transforming such raw sequence data into knowledge remains a hard task. A great number of prediction programs have...

Genome-Wide Analysis of Core Cell Cycle Genes in Arabidopsis

Vandepoele, Klaas, Raes, Jeroen, De Veylder, Lieven, Rouzé, Pierre, Rombauts, Stephane, Inzé, Dirk

Cyclin-dependent kinases and cyclins regulate with the help of different interacting proteins the progression through the eukaryotic cell cycle. A high-quality, homology-based annotation protocol was...

CATMA: a complete Arabidopsis GST database

Crowe, Mark L., Serizet, Carine, Thareau, Vincent, Aubourg, Sébastien, Rouzé, Pierre, Hilson, Pierre, ...

The Complete Arabidopsis Transcriptome Micro Array (CATMA) database contains gene sequence tag (GST) and gene model sequences for over 70% of the predicted genes in the Arabidopsis thaliana genome as...

Computational Approaches to Identify Promoters and cis-Regulatory Elements in Plant Genomes1

Rombauts, Stephane, Florquin, Kobe, Lescot, Magali, Marchal, Kathleen, Rouzé, Pierre, Van De Peer, Yves

The identification of promoters and their regulatory elements is one of the major challenges in bioinformatics and integrates comparative, structural, and functional genomics. Many different...

Detection of 91 potential conserved plant microRNAs in Arabidopsis thaliana and Oryza sativa identifies important target genes

Bonnet, Eric, Wuyts, Jan, Rouzé, Pierre, Van De Peer, Yves

MicroRNAs (miRNAs) are an extensive class of tiny RNA molecules that regulate the expression of target genes by means of complementary base pair interactions. Although the first miRNAs were...

Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications

Hilson, Pierre, Allemeersch, Joke, Altmann, Thomas, Aubourg, Sébastien, Avon, Alexandra, Beynon, Jim, ...

Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization...

GeneFarm, structural and functional annotation of Arabidopsis gene and protein families by a network of experts

Aubourg, Sébastien, Brunaud, Véronique, Bruyère, Clémence, Cock, Mark, Cooke, Richard, Cottet, Annick, ...

Genomic projects heavily depend on genome annotations and are limited by the current deficiencies in the published predictions of gene structure and function. It follows that, improved annotation...

Large-scale structural analysis of the core promoter in mammalian and plant genomes

Florquin, Kobe, Saeys, Yvan, Degroeve, Sven, Rouzé, Pierre, Van De Peer, Yves

DNA encodes at least two independent levels of functional information. The first level is for encoding proteins and sequence targets for DNA-binding factors, while the second one is contained in the...

Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features

Derelle, Evelyne, Ferraz, Conchita, Rombauts, Stephane, Rouzé, Pierre, Worden, Alexandra Z., Robbens, Steven, ...

The green lineage is reportedly 1,500 million years old, evolving shortly after the endosymbiosis event that gave rise to early photosynthetic eukaryotes. In this study, we unveil the complete genome...

The tiny eukaryote Ostreococcus provides genomic insights into the paradox of plankton speciation

Palenik, Brian, Grimwood, Jane, Aerts, Andrea, Rouzé, Pierre, Salamov, Asaf, Putnam, Nicholas, ...

The smallest known eukaryotes, at ≈1-μm diameter, are Ostreococcus tauri and related species of marine phytoplankton. The genome of Ostreococcus lucimarinus has been completed and compared with...

Generic eukaryotic core promoter prediction using structural features of DNA

Abeel, Thomas, Saeys, Yvan, Bonnet, Eric, Rouzé, Pierre, Van De Peer, Yves

Despite many recent efforts, in silico identification of promoter regions is still in its infancy. However, the accurate identification and delineation of promoter regions is important for several...

Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress

Dittami, Simon M, Scornet, Delphine, Petit, Jean-Louis, Ségurens, Béatrice, Da Silva, Corinne, Corre, Erwan, ...

The brown alga Ectocarpus siliculosus, unlike terrestrial plants, undergoes extensive reprogramming of its transcriptome during the acclimation to mild abiotic stress.

ProSOM: core promoter prediction based on unsupervised clustering of DNA physical profiles

Abeel, Thomas, Saeys, Yvan, Rouzé, Pierre, Van De Peer, Yves

Motivation: More and more genomes are being sequenced, and to keep up with the pace of sequencing projects, automated annotation techniques are required. One of the most challenging problems in...

Open access to sequence: Browsing the Pichia pastoris genome

Mattanovich, Diethard, Callewaert, Nico, Rouzé, Pierre, Lin, Yao-Cheng, Graf, Alexandra, Redl, Andreas, ...

The first genome sequences of the important yeast protein production host Pichia pastoris have been released into the public domain this spring. In order to provide the scientific community easy and...