C. Francke, P. W. Postma, H. V. Westerhoff, J. G. Blom, M. A. Peletier, Christof Francke, ...
Why the phosphotransferase system of Escherichia coli escapes the diffusion limitation of signal transduction, transport and metabolism that confronts mammalian cells
Postma, Pieter W., Gunnewijk, Marga G.W., Poolman, Bert
The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the...
Poolman, Bert, Postma, Pieter W., Gunnewijk, Marga G.W.
The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the...
Chen, Ruizhen, Hatzimanikatis, Vassily, Postma, Pieter W., Bailey, James E.
E. coli strain PPA305, which has a wild-type PTS system, and PPA316, which utilizes a proton-galactose symport system for glucose uptake, were used as host strains to harbor a phenylalanine...
Hoff, Wouter D., Sprenger, Wander W., Postma, Pieter W., Meyer, Terry E., Veenhuis, Marten, Leguijt, Tina, ...
A rabbit antiserum was raised against the photoactive yellow protein (PYP) from Ectothiorhodospira halophila and purified by adsorption experiments to obtain a highly specific polyclonal antiserum....
Postma, Pieter W., Blaauw, Mieke, Misset, Onno, Robillard, George T.
The phosphoryl-group transfer from PHPr to glucose or α-methylglucose and from glucose 6-phosphate to these same sugars catalyzed by membrane-bound EIIBGlc of the bacterial...
Postma, Pieter W., Blaauw, Mieke, Misset, Onno, Robillard, George T.
The phosphoryl-group transfer from PHPr to glucose or α-methylglucose and from glucose 6-phosphate to these same sugars catalyzed by membrane-bound EIIBGlc of the bacterial...
Implications of macromolecular crowding for signal transduction and metabolite channeling
Rohwer, Johann M., Postma, Pieter W., Kholodenko, Boris N., Westerhoff, Hans V.
The effect of different total enzyme concentrations on the flux through the bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in vitro was determined by measuring...
Chaillou, Stéphane, Bor, Yeou-Cherng, Batt, Carl A., Postma, Pieter W., Pouwels, Peter H.
A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding d-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could...
Gunnewijk, Marga G. W., Postma, Pieter W., Poolman, Bert
The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the...
Chaillou, Stéphane, Pouwels, Peter H., Postma, Pieter W.
We have identified and characterized the d-xylose transport system of Lactobacillus pentosus. Uptake of d-xylose was not driven by the proton motive force generated by malolactic fermentation and...
Autoamplification of a Two-Component Regulatory System Results in “Learning” Behavior
Hoffer, Sally M., Westerhoff, Hans V., Hellingwerf, Klaas J., Postma, Pieter W., Tommassen, Jan
We have tested the hypothesis that the autoamplification of two-component regulatory systems results in “learning” behavior, i.e., that bacteria respond faster or more extensively to a signal...
Chen, Qing, Postma, Pieter W., Amster-Choder, Orna
The Escherichia coli BglF protein catalyzes transport and phosphorylation of β-glucosides. In addition, BglF is a membrane sensor which reversibly phosphorylates the transcriptional regulator BglG,...
Chaillou, Stéphane, Lokman, B. Christien, Leer, Rob J., Posthuma, Clara, Postma, Pieter W., Pouwels, Peter H.
Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is...
Chaillou, Stéphane, Postma, Pieter W., Pouwels, Peter H.
The xylP gene of Lactobacillus pentosus, the first gene of the xylPQR operon, was recently found to be involved in isoprimeverose metabolism. By expression of xylP on a multicopy plasmid in...
Monedero, Vicente, Postma, Pieter W., Pérez-Martínez, Gaspar
A DNA fragment from Lactobacillus casei that restores growth to Escherichia coli and Salmonella typhimurium ptsH mutants on glucose and other substrates of the phosphoenolpyruvate:carbohydrate...
Butler, Michael J., Bruheim, Per, Jovetic, Srdjan, Marinelli, Flavia, Postma, Pieter W., Bibb, Mervyn J.
Deletions were made in Streptomyces lividans in either of two genes (zwf1 and zwf2) encoding isozymes of glucose-6-phosphate dehydrogenase, the first enzyme in the oxidative pentose phosphate pathway...
Posthuma, Clara C., Bader, Rechien, Engelmann, Roswitha, Postma, Pieter W., Hengstenberg, Wolfgang, Pouwels, Peter H.
Purification of xylulose 5-phosphate phosphoketolase (XpkA), the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene,...
Cooperativity in Signal Transfer through the Uhp System of Escherichia coli
Verhamme, Daniël T., Postma, Pieter W., Crielaard, Wim, Hellingwerf, Klaas J.
The UhpABC regulatory system in enterobacteria controls the expression of the hexose phosphate transporter UhpT. Signaling is initiated through sensing of extracellular glucose 6-phosphate by...
Why the Phosphotransferase System of Escherichia coli Escapes Diffusion Limitation
Francke, Christof, Postma, Pieter W., Westerhoff, Hans V., Blom, Joke G., Peletier, Mark A.
We calculated the implications of diffusion for the phosphoenolpyruvate:glucose phosphotransferase system (glucose-PTS) of Escherichia coli in silicon cells of various magnitudes. For a cell of...
Implications of macromolecular crowding for signal transduction and metabolite channeling
Rohwer, Johann M., Postma, Pieter W., Kholodenko, Boris N., Westerhoff, Hans V.
The effect of different total enzyme concentrations on the flux through the bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in vitro was determined by measuring...
Chaillou, Stéphane, Bor, Yeou-Cherng, Batt, Carl A., Postma, Pieter W., Pouwels, Peter H.
A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding d-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could...
Gunnewijk, Marga G. W., Postma, Pieter W., Poolman, Bert
The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the...
Chaillou, Stéphane, Pouwels, Peter H., Postma, Pieter W.
We have identified and characterized the d-xylose transport system of Lactobacillus pentosus. Uptake of d-xylose was not driven by the proton motive force generated by malolactic fermentation and...
Autoamplification of a Two-Component Regulatory System Results in “Learning” Behavior
Hoffer, Sally M., Westerhoff, Hans V., Hellingwerf, Klaas J., Postma, Pieter W., Tommassen, Jan
We have tested the hypothesis that the autoamplification of two-component regulatory systems results in “learning” behavior, i.e., that bacteria respond faster or more extensively to a signal...
Chen, Qing, Postma, Pieter W., Amster-Choder, Orna
The Escherichia coli BglF protein catalyzes transport and phosphorylation of β-glucosides. In addition, BglF is a membrane sensor which reversibly phosphorylates the transcriptional regulator BglG,...
Chaillou, Stéphane, Lokman, B. Christien, Leer, Rob J., Posthuma, Clara, Postma, Pieter W., Pouwels, Peter H.
Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is...
Chaillou, Stéphane, Postma, Pieter W., Pouwels, Peter H.
The xylP gene of Lactobacillus pentosus, the first gene of the xylPQR operon, was recently found to be involved in isoprimeverose metabolism. By expression of xylP on a multicopy plasmid in...
Monedero, Vicente, Postma, Pieter W., Pérez-Martínez, Gaspar
A DNA fragment from Lactobacillus casei that restores growth to Escherichia coli and Salmonella typhimurium ptsH mutants on glucose and other substrates of the phosphoenolpyruvate:carbohydrate...
Butler, Michael J., Bruheim, Per, Jovetic, Srdjan, Marinelli, Flavia, Postma, Pieter W., Bibb, Mervyn J.
Deletions were made in Streptomyces lividans in either of two genes (zwf1 and zwf2) encoding isozymes of glucose-6-phosphate dehydrogenase, the first enzyme in the oxidative pentose phosphate pathway...
Posthuma, Clara C., Bader, Rechien, Engelmann, Roswitha, Postma, Pieter W., Hengstenberg, Wolfgang, Pouwels, Peter H.
Purification of xylulose 5-phosphate phosphoketolase (XpkA), the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene,...
Cooperativity in Signal Transfer through the Uhp System of Escherichia coli
Verhamme, Daniël T., Postma, Pieter W., Crielaard, Wim, Hellingwerf, Klaas J.
The UhpABC regulatory system in enterobacteria controls the expression of the hexose phosphate transporter UhpT. Signaling is initiated through sensing of extracellular glucose 6-phosphate by...
Why the Phosphotransferase System of Escherichia coli Escapes Diffusion Limitation
Francke, Christof, Postma, Pieter W., Westerhoff, Hans V., Blom, Joke G., Peletier, Mark A.
We calculated the implications of diffusion for the phosphoenolpyruvate:glucose phosphotransferase system (glucose-PTS) of Escherichia coli in silicon cells of various magnitudes. For a cell of...
Deutscher, Josef, Francke, Christof, Postma, Pieter W.
The phosphoenolpyruvate(PEP):carbohydrate phosphotransferase system (PTS) is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides,...