Impairment of Human Neutrophil Oxidative Burst By (2008)
Polychlorinated Biphenyls Inhibition, P. K. Narayanan, W. O. Carter, P. E. Ganey, R. A. Roth, ...
We report evidence of a novel mechanism by which polychlorinated biphenyls might act as potent inducers of inflammation. Aroclor 1242 (A1242), a polychlorinated biphenyl mixture, and...
Systematic Evaluation of Cellular Radiosensitivity Parameters (1976)
Roth, R. A., Sharma, S. C., Katz, Robert
Cellular radiosensitivity parameters of the track structure theory of Katz and co-workers are evaluated from a sum of squares minimizing computer program for nonlinear models. Based on these...
Simplified RBE-Dose Calculations for Mixed Radiation Fields (1976)
Katz, Robert, Fullerton, B. G., Roth, R. A., Sharma, S. C.
A problem for radiobiology, radiation protection and treatment planning is the calculation of RBE as a function of the delivered dose when this dose is a result of a mixture of low and high LET...
To identify potential signaling molecules involved in mediating insulin-induced biological responses, a yeast two-hybrid screen was performed with the cytoplasmic domain of the human insulin receptor...
Molecular basis of the activation of the tumorigenic potential of Gag-insulin receptor chimeras.
Poon, B, Dixon, D, Ellis, L, Roth, R A, Rutter, W J, Wang, L H
A previous study showed that the human insulin receptor (IR) could be activated by insertion of a 3' portion of the cDNA encoding the beta subunit into a retrovirus genome to form a Gag-IR fusion...
Chimeric receptors containing different portions of the homologous human insulin receptor, insulin-like growth factor I receptor, and insulin receptor-related receptor were utilized to identify the...
Hansen, T, Stagsted, J, Pedersen, L, Roth, R A, Goldstein, A, Olsson, L
Peptides from the alpha 1 region (residues 61-85) of the D and K molecules of the major histocompatibility complex class I antigens inhibit insulin-induced tyrosine kinase activity of the purified...
Bondy, C A, Zhou, J, Chin, E, Reinhardt, R R, Ding, L, Roth, R A
Insulin-degrading enzyme (IDE) hydrolyzes both insulin and IGFs and has been proposed to play a role in signal termination after binding of these peptides to their receptors. In situ hybridization...
Ellis, L, Morgan, D O, Jong, S M, Wang, L H, Roth, R A, Rutter, W J
A hybrid receptor molecule composed of the extracellular ligand-binding domain of the human insulin receptor and the transmembrane and cytoplasmic (protein-tyrosine kinase) domains of the chicken...
Activation of transforming potential of the human insulin receptor gene.
Wang, L H, Lin, B, Jong, S M, Dixon, D, Ellis, L, Roth, R A, ...
A retrovirus containing part of the human insulin receptor (hIR) gene was constructed by replacing ros sequences in the avian sarcoma virus UR2 with hIR cDNA sequences coding for 46 amino acids of...
The role of the insulin receptor tyrosine kinase (protein-tyrosine kinase, EC 2.7.1.112) in various rapid insulin effects was studied by injecting four different cell types (by osmotic lysis of...
Ebina, Y, Araki, E, Taira, M, Shimada, F, Mori, M, Craik, C S, ...
To test whether the tyrosine kinase activity of the insulin receptor is crucial for insulin action, we have constructed mutations of the human insulin receptor at Lys-1030, which is in the presumed...
Insulin action is blocked by a monoclonal antibody that inhibits the insulin receptor kinase.
Morgan, D O, Ho, L, Korn, L J, Roth, R A
Thirty-six monoclonal antibodies to the human insulin receptor were produced. Thirty-four bound the intracellular domain of the receptor beta subunit, the domain containing the tyrosine-specific...
Four monoclonal antibodies were identified by their ability to bind to 125I-labeled insulin covalently linked to a cytosolic insulin-degrading enzyme from human erythrocytes. All four antibodies were...
Roth, R A, Cassell, D J, Wong, K Y, Maddux, B A, Goldfine, I D
Antibodies to the insulin receptor were prepared in BALB/c mice by immunization with IM-9 human lymphocytes, a cell type that has a large number of plasma membrane insulin receptors. The spleens of...
Bollag, G E, Roth, R A, Beaudoin, J, Mochly-Rosen, D, Koshland, D E
The beta subunit of purified insulin receptor is phosphorylated on a serine residue by purified preparations of protein kinase C (ATP: protein phosphotransferase, EC 2.7.1.37). This phosphorylation...
Linking functional domains of the human insulin receptor with the bacterial aspartate receptor.
Ellis, L, Morgan, D O, Koshland, D E, Clauser, E, Moe, G R, Bollag, G, ...
A hybrid receptor has been constructed that is composed of the extracellular domain of the human insulin receptor fused to the transmembrane and cytoplasmic domains of the bacterial aspartate...
Expression of a functional human insulin receptor from a cloned cDNA in Chinese hamster ovary cells.
Ebina, Y, Edery, M, Ellis, L, Standring, D, Beaudoin, J, Roth, R A, ...
We have placed human insulin receptor cDNA into a vector under the control of the simian virus 40 (SV40) early promoter and tested its function by transient expression in microinjected Xenopus...
Kohn, A D, Kovacina, K S, Roth, R A
In the present study, insulin is shown to rapidly stimulate by 8- to 12-fold the enzymatic activity of RAC-PK alpha, a pleckstrin homology domain containing ser/thr kinase. In contrast, activation of...
Verspohl, E J, Roth, R A, Vigneri, R, Goldfine, I D
Insulin and the insulinlike growth factors (IGF-I and IGF-II) are members of a family of hormones that regulate the metabolism and growth of many tissues. Cultured HEP-G2 cells (a minimal deviation...
An unusual active site identified in a family of zinc metalloendopeptidases.
An unusual active site has been identified in a family of zinc metalloendopeptidases that includes bacterial protease III and the human and Drosophila insulin-degrading enzymes. All of these enzymes...
The receptor for insulin-like growth factor II mediates an insulin-like response.
Hari, J, Pierce, S B, Morgan, D O, Sara, V, Smith, M C, Roth, R A
Insulin-like growth factor II (IGF-II) shares sequence homology and predicted three-dimensional structure with insulin and IGF-I. IGF-II can bind, therefore, to a limited extent with the receptors...
Dickens, M, Chin, J E, Roth, R A, Ellis, L, Denton, R M, Tavaré, J M
The activation of insulin-stimulated protein-serine/threonine kinases has been investigated in CHO cell lines transfected with cDNAs encoding either wild-type or mutant human insulin receptors. (1)...
A novel active site has been identified in a family of zinc-dependent metalloendopeptidases that includes bacterial proteinase III, the human and Drosophila insulin-degrading enzymes, and the...
A line of Chinese hamster ovary cells overexpressing protein kinase C alpha was transfected with cDNAs encoding either the wild-type human insulin receptor or one of two mutant insulin receptors with...
Holland, S J, Gale, N W, Gish, G D, Roth, R A, Songyang, Z, Cantley, L C, ...
Eph-related receptor tyrosine kinases have been implicated in the control of axonal navigation and fasciculation. To investigate the biochemical mechanisms underlying such functions, we have...
Iynedjian, P B, Roth, R A, Fleischmann, M, Gjinovci, A
Inhibitors of signalling pathways were used to dissect the mechanism of insulin action on expression of the gene encoding glucokinase in cultured rat hepatocytes. Wortmannin and LY 294002 completely...
Lipopolysaccharide-induced hepatic injury is enhanced by polychlorinated biphenyls.
Brown, A P, Schultze, A E, Holdan, W L, Buchweitz, J P, Roth, R A, Ganey, P E
After intravenous administration of bacterial lipopolysaccharide (LPS) to rats, polymorphonuclear neutrophils (PMNs) rapidly accumulate in the liver, and midzonal hepatic necrosis is prominent by 6...
Characterization of acute 4,4'-methylene dianiline hepatotoxicity in the rat.
Bailie, M B, Mullaney, T P, Roth, R A
Methylene dianiline (DDM) is a chemical intermediate in the production of isocyanates and other industrial chemicals, and it is hepatotoxic in humans. The acute hepatotoxicity of orally administered...
Neutrophil function after exposure to polychlorinated biphenyls in vitro.
Ganey, P E, Sirois, J E, Denison, M, Robinson, J P, Roth, R A
Polychlorinated biphenyls (PCBs) are known to be immunotoxic, yet the effects on neutrophil (PMN) function are not well characterized. We incubated PMNs isolated from rat peritoneum with a mixture of...
To identify potential signaling molecules involved in mediating insulin-induced biological responses, a yeast two-hybrid screen was performed with the cytoplasmic domain of the human insulin receptor...
Molecular basis of the activation of the tumorigenic potential of Gag-insulin receptor chimeras.
Poon, B, Dixon, D, Ellis, L, Roth, R A, Rutter, W J, Wang, L H
A previous study showed that the human insulin receptor (IR) could be activated by insertion of a 3' portion of the cDNA encoding the beta subunit into a retrovirus genome to form a Gag-IR fusion...
Chimeric receptors containing different portions of the homologous human insulin receptor, insulin-like growth factor I receptor, and insulin receptor-related receptor were utilized to identify the...
Hansen, T, Stagsted, J, Pedersen, L, Roth, R A, Goldstein, A, Olsson, L
Peptides from the alpha 1 region (residues 61-85) of the D and K molecules of the major histocompatibility complex class I antigens inhibit insulin-induced tyrosine kinase activity of the purified...
Bondy, C A, Zhou, J, Chin, E, Reinhardt, R R, Ding, L, Roth, R A
Insulin-degrading enzyme (IDE) hydrolyzes both insulin and IGFs and has been proposed to play a role in signal termination after binding of these peptides to their receptors. In situ hybridization...
Ellis, L, Morgan, D O, Jong, S M, Wang, L H, Roth, R A, Rutter, W J
A hybrid receptor molecule composed of the extracellular ligand-binding domain of the human insulin receptor and the transmembrane and cytoplasmic (protein-tyrosine kinase) domains of the chicken...
Activation of transforming potential of the human insulin receptor gene.
Wang, L H, Lin, B, Jong, S M, Dixon, D, Ellis, L, Roth, R A, ...
A retrovirus containing part of the human insulin receptor (hIR) gene was constructed by replacing ros sequences in the avian sarcoma virus UR2 with hIR cDNA sequences coding for 46 amino acids of...
The role of the insulin receptor tyrosine kinase (protein-tyrosine kinase, EC 2.7.1.112) in various rapid insulin effects was studied by injecting four different cell types (by osmotic lysis of...
Ebina, Y, Araki, E, Taira, M, Shimada, F, Mori, M, Craik, C S, ...
To test whether the tyrosine kinase activity of the insulin receptor is crucial for insulin action, we have constructed mutations of the human insulin receptor at Lys-1030, which is in the presumed...
Insulin action is blocked by a monoclonal antibody that inhibits the insulin receptor kinase.
Morgan, D O, Ho, L, Korn, L J, Roth, R A
Thirty-six monoclonal antibodies to the human insulin receptor were produced. Thirty-four bound the intracellular domain of the receptor beta subunit, the domain containing the tyrosine-specific...
Four monoclonal antibodies were identified by their ability to bind to 125I-labeled insulin covalently linked to a cytosolic insulin-degrading enzyme from human erythrocytes. All four antibodies were...
Roth, R A, Cassell, D J, Wong, K Y, Maddux, B A, Goldfine, I D
Antibodies to the insulin receptor were prepared in BALB/c mice by immunization with IM-9 human lymphocytes, a cell type that has a large number of plasma membrane insulin receptors. The spleens of...
Bollag, G E, Roth, R A, Beaudoin, J, Mochly-Rosen, D, Koshland, D E
The beta subunit of purified insulin receptor is phosphorylated on a serine residue by purified preparations of protein kinase C (ATP: protein phosphotransferase, EC 2.7.1.37). This phosphorylation...
Linking functional domains of the human insulin receptor with the bacterial aspartate receptor.
Ellis, L, Morgan, D O, Koshland, D E, Clauser, E, Moe, G R, Bollag, G, ...
A hybrid receptor has been constructed that is composed of the extracellular domain of the human insulin receptor fused to the transmembrane and cytoplasmic domains of the bacterial aspartate...
Expression of a functional human insulin receptor from a cloned cDNA in Chinese hamster ovary cells.
Ebina, Y, Edery, M, Ellis, L, Standring, D, Beaudoin, J, Roth, R A, ...
We have placed human insulin receptor cDNA into a vector under the control of the simian virus 40 (SV40) early promoter and tested its function by transient expression in microinjected Xenopus...
Kohn, A D, Kovacina, K S, Roth, R A
In the present study, insulin is shown to rapidly stimulate by 8- to 12-fold the enzymatic activity of RAC-PK alpha, a pleckstrin homology domain containing ser/thr kinase. In contrast, activation of...
Verspohl, E J, Roth, R A, Vigneri, R, Goldfine, I D
Insulin and the insulinlike growth factors (IGF-I and IGF-II) are members of a family of hormones that regulate the metabolism and growth of many tissues. Cultured HEP-G2 cells (a minimal deviation...
An unusual active site identified in a family of zinc metalloendopeptidases.
An unusual active site has been identified in a family of zinc metalloendopeptidases that includes bacterial protease III and the human and Drosophila insulin-degrading enzymes. All of these enzymes...
The receptor for insulin-like growth factor II mediates an insulin-like response.
Hari, J, Pierce, S B, Morgan, D O, Sara, V, Smith, M C, Roth, R A
Insulin-like growth factor II (IGF-II) shares sequence homology and predicted three-dimensional structure with insulin and IGF-I. IGF-II can bind, therefore, to a limited extent with the receptors...
Dickens, M, Chin, J E, Roth, R A, Ellis, L, Denton, R M, Tavaré, J M
The activation of insulin-stimulated protein-serine/threonine kinases has been investigated in CHO cell lines transfected with cDNAs encoding either wild-type or mutant human insulin receptors. (1)...
A novel active site has been identified in a family of zinc-dependent metalloendopeptidases that includes bacterial proteinase III, the human and Drosophila insulin-degrading enzymes, and the...
A line of Chinese hamster ovary cells overexpressing protein kinase C alpha was transfected with cDNAs encoding either the wild-type human insulin receptor or one of two mutant insulin receptors with...
Holland, S J, Gale, N W, Gish, G D, Roth, R A, Songyang, Z, Cantley, L C, ...
Eph-related receptor tyrosine kinases have been implicated in the control of axonal navigation and fasciculation. To investigate the biochemical mechanisms underlying such functions, we have...
Iynedjian, P B, Roth, R A, Fleischmann, M, Gjinovci, A
Inhibitors of signalling pathways were used to dissect the mechanism of insulin action on expression of the gene encoding glucokinase in cultured rat hepatocytes. Wortmannin and LY 294002 completely...
Lipopolysaccharide-induced hepatic injury is enhanced by polychlorinated biphenyls.
Brown, A P, Schultze, A E, Holdan, W L, Buchweitz, J P, Roth, R A, Ganey, P E
After intravenous administration of bacterial lipopolysaccharide (LPS) to rats, polymorphonuclear neutrophils (PMNs) rapidly accumulate in the liver, and midzonal hepatic necrosis is prominent by 6...
Characterization of acute 4,4'-methylene dianiline hepatotoxicity in the rat.
Bailie, M B, Mullaney, T P, Roth, R A
Methylene dianiline (DDM) is a chemical intermediate in the production of isocyanates and other industrial chemicals, and it is hepatotoxic in humans. The acute hepatotoxicity of orally administered...
Neutrophil function after exposure to polychlorinated biphenyls in vitro.
Ganey, P E, Sirois, J E, Denison, M, Robinson, J P, Roth, R A
Polychlorinated biphenyls (PCBs) are known to be immunotoxic, yet the effects on neutrophil (PMN) function are not well characterized. We incubated PMNs isolated from rat peritoneum with a mixture of...
Monocrotaline pyrrole (MCTP), a reactive electrophile, induces delayed and progressive pulmonary edema, vascular remodeling, and pulmonary hypertension after a single intravenous administration to...
Dahm, L. J., Schultze, A. E., Roth, R. A.
Under certain circumstances, segmented neutrophils (PMNs) injure extrahepatic tissue by releasing toxic oxygen species and degradative enzymes. The authors used an isolated, perfused rat liver...
The effects of intraperitoneal hydrallazine, dexamethasone, or sulphinpyrazone on the toxicity of monocrotaline pyrrole (MCTP) were examined in rats 14 days after injection of MCTP (5 mg kg-1, i.v.)....
1. Changes in the structural and functional integrity of endothelium have been recognized as relatively early features of delayed and progressive pulmonary vascular injury caused by the pyrrolizidine...