A Chimaeric ribonuclease-inhibitor gene restores fertility to male sterile plants (1992)
Mariani, C., Leemans, J., Degreef, W., Goldberg, R.B., Deblock, M., Debeuckeleer, M., ...
Induction of male sterility in plants by a chimaeric ribonuclease gene (1990)
Mariani, C., Goldberg, R.B., Treuttner, J., De Beuckeleer, M.
Imprinting of the MEDEA polycomb gene in the Arabidopsis endosperm.
Kinoshita, T, Yadegari, R, Harada, J J, Goldberg, R B, Fischer, R L
In flowering plants, two cells are fertilized in the haploid female gametophyte. Egg and sperm nuclei fuse to form the embryo. A second sperm nucleus fuses with the central cell nucleus that...
Mutations in FIE, a WD polycomb group gene, allow endosperm development without fertilization.
Ohad, N, Yadegari, R, Margossian, L, Hannon, M, Michaeli, D, Harada, J J, ...
A fundamental problem in biology is to understand how fertilization initiates reproductive development. Higher plant reproduction is unique because two fertilization events are required for sexual...
A novel cell ablation strategy blocks tobacco anther dehiscence.
We utilized a new cell ablation strategy to ablate specific anther cell types involved in the dehiscence process. The tobacco TA56 gene promoter is active within the circular cell cluster, stomium,...
Characterization of the glycinin gene family in soybean.
Nielsen, N C, Dickinson, C D, Cho, T J, Thanh, V H, Scallon, B J, Fischer, R L, ...
We characterized the structure, organization, and expression of genes that encode the soybean glycinins, a family of storage proteins synthesized exclusively in seeds during embryogenesis. Five genes...
Cho, T J, Davies, C S, Fischer, R L, Turner, N E, Goldberg, R B, Nielsen, N C
The soybean variety Forrest contains an aberrant allele for the Gy3 glycinin gene. The aberrant allele is designated gy3 because mRNA for the G3 glycinin subunit is reduced to below detectable...
Harada, J J, Barker, S J, Goldberg, R B
We investigated the chromosomal organization and developmental regulation of soybean beta-conglycinin genes. The beta-conglycinin gene family contains at least 15 members divided into two major...
A frameshift mutation prevents Kunitz trypsin inhibitor mRNA accumulation in soybean embryos.
Jofuku, K D, Schipper, R D, Goldberg, R B
We investigated the molecular basis of a soybean Kunitz trypsin inhibitor (KTi) gene mutation that prevents the accumulation of Kunitz trypsin inhibitor protein during seed development. We found that...
We investigated the structure, organization, and developmental regulation of soybean Kunitz trypsin inhibitor genes. The Kunitz trypsin inhibitor gene family contains at least 10 members, many of...
Soybean Seed Protein Genes Are Regulated Spatially during Embryogenesis.
Perez-Grau, L., Goldberg, R. B.
We used in situ hybridization to investigate Kunitz trypsin inhibitor gene expression programs at the cell level in soybean embryos and in transformed tobacco seeds. The major Kunitz trypsin...
A mutant lectin gene is rescued from an insertion element that blocks its expression.
The soybean lectin gene Le1 encodes a prevalent seed protein and is highly regulated during the life cycle. The mutant lectin gene allele le1 is not transcribed detectably, contains a 3.5-kb Tgm1...
Regional and cell-specific gene expression patterns during petal development.
Drews, G N, Beals, T P, Bui, A Q, Goldberg, R B
We investigated gene expression patterns that occur during tobacco petal development. Two petal mRNA classes were identified that are present at elevated levels relative to other organs. One class is...
Paradiso, M J, Roberts, G, Streicher, S L, Goldberg, R B
The viomycin phosphotransferase gene (vph) is expressed and confers resistance to viomycin in both Streptomyces spp. and members of the family Enterobacteriaceae. We report the isolation of UGA...
We have partially characterized the biochemical parameters of glutamine synthetase from Klebsiella pneumoniae and have shown that the differential affinity of adenylylated and unadenylylated...
Regulation of histidase synthesis in intergeneric hybrids of enteric bacteria.
Goldberg, R B, Bloom, F R, Magasanik, B
Regulation of the expression of the histidase coded by hutk of Klebsiella aerogenes in Salmonella typhimurium and in Escherichia coli and of the expression of the histidase coded by huts of S....
Transduction of chromosomal genes between enteric bacteria by bacteriophage P1.
We have used P1 transduction to create intergeneric hybrid strains of enteric bacteria by moving the genA and hut genes between Klebsiella aerogenes, Escherichia coli and Salmonella typhimurium. The...
Gene order of the histidine utilization (hut) operons in Klebsiella aerogenes.
P1-sensitive mutants of Klebsiella aerogenes were isolated and the gene order of the hut region was then determined using P1-mediated transduction. The genes are located in the Klebsiella chromosome...
Regulation of Klebsiella pneumoniae hut operons by oxygen.
We investigated the regulation of genes concerned with nitrogen metabolism by oxygen in the facultative anaerobe Klebsiella pneumoniae. We found oxygen to be required for the expression of the hut...
Regulation of nitrogen metabolism in glutamine auxotrophs of Klebsiella pneumoniae.
We examined the regulation of nitrogen metabolism in four classes (glnA, glnB, glnF, and glnG) of Gln- auxotrophs of Klebsiella pneumoniae. These studies indicate that glutamine synthetase does not...
Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group.
Cornelissen, B J, Horowitz, J, Van Kan, J A, Goldberg, R B, Bol, J F
Infection of Samsun NN tobacco with tobacco mosaic virus (TMV) was found to induce the synthesis of mRNA encoding a basic protein with a 67% amino acid sequence homology to the known acidic...
Organ-specific nuclear RNAs in tobacco.
We investigated the developmental regulation of nuclear RNA sequences in tobacco vegetative (leaf, root, stem) and floral (petal, ovary, anther) organ systems using RNA-excess X single-copy DNA...
Electron microscopy of Achlya deoxyribonucleic acid sequence organization.
Pellegrini, M, Timberlake, W E, Goldberg, R B
Electron microscopic analysis of reassociated deoxyribonucleic acid (DNA) from the aquatic fungus Achlya bisexualis revealed details of the sequence arrangement of the inverted repeats and both the...
Okamuro, J K, Jofuku, K D, Goldberg, R B
We introduced a 17.1-kilobase soybean DNA fragment containing the lectin gene and at least four nonseed protein genes into the tobacco genome. As in soybean plants, lectin mRNA is present in tobacco...
Female sterile tobacco plants are produced by stigma-specific cell ablation.
Goldman, M H, Goldberg, R B, Mariani, C
We identified a tobacco stigma-specific gene, designated STIG1. The STIG1 gene is developmentally regulated and expressed specifically in the stigmatic secretory zone. We used a chimeric STIG1-GUS...
Stacpoole, P W, Bridge, D M, Alvarez, I M, Goldberg, R B, Harwood, H J
3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoA reductase) controls the rate of cholesterol biosynthesis and is itself modulated through feedback suppression by internalized low density...
Joffe, B I, Goldberg, R B, Feinstein, J, Kark, A, Seftel, H C
Aspects of adipose tissue cellularity were examined in 15 non-diabetic premenopausal African women with simple obesity living in Johannesburg. A smaller group of six non-obese Black women served as...
Spondyloepiphyseal dysplasia congenita: genetic linkage to type II collagen (COL2AI).
Anderson, I J, Goldberg, R B, Marion, R W, Upholt, W B, Tsipouras, P
Spondyloepiphyseal dysplasia congenita (SEDC) is an autosomal dominantly inherited chondrodysplasia characterized by disproportionate short stature (short trunk), abnormal epiphyses, and flattened...
Imprinting of the MEDEA polycomb gene in the Arabidopsis endosperm.
Kinoshita, T, Yadegari, R, Harada, J J, Goldberg, R B, Fischer, R L
In flowering plants, two cells are fertilized in the haploid female gametophyte. Egg and sperm nuclei fuse to form the embryo. A second sperm nucleus fuses with the central cell nucleus that...
Mutations in FIE, a WD polycomb group gene, allow endosperm development without fertilization.
Ohad, N, Yadegari, R, Margossian, L, Hannon, M, Michaeli, D, Harada, J J, ...
A fundamental problem in biology is to understand how fertilization initiates reproductive development. Higher plant reproduction is unique because two fertilization events are required for sexual...
A novel cell ablation strategy blocks tobacco anther dehiscence.
We utilized a new cell ablation strategy to ablate specific anther cell types involved in the dehiscence process. The tobacco TA56 gene promoter is active within the circular cell cluster, stomium,...
Characterization of the glycinin gene family in soybean.
Nielsen, N C, Dickinson, C D, Cho, T J, Thanh, V H, Scallon, B J, Fischer, R L, ...
We characterized the structure, organization, and expression of genes that encode the soybean glycinins, a family of storage proteins synthesized exclusively in seeds during embryogenesis. Five genes...
Cho, T J, Davies, C S, Fischer, R L, Turner, N E, Goldberg, R B, Nielsen, N C
The soybean variety Forrest contains an aberrant allele for the Gy3 glycinin gene. The aberrant allele is designated gy3 because mRNA for the G3 glycinin subunit is reduced to below detectable...
Harada, J J, Barker, S J, Goldberg, R B
We investigated the chromosomal organization and developmental regulation of soybean beta-conglycinin genes. The beta-conglycinin gene family contains at least 15 members divided into two major...
A frameshift mutation prevents Kunitz trypsin inhibitor mRNA accumulation in soybean embryos.
Jofuku, K D, Schipper, R D, Goldberg, R B
We investigated the molecular basis of a soybean Kunitz trypsin inhibitor (KTi) gene mutation that prevents the accumulation of Kunitz trypsin inhibitor protein during seed development. We found that...
We investigated the structure, organization, and developmental regulation of soybean Kunitz trypsin inhibitor genes. The Kunitz trypsin inhibitor gene family contains at least 10 members, many of...
Soybean Seed Protein Genes Are Regulated Spatially during Embryogenesis.
Perez-Grau, L., Goldberg, R. B.
We used in situ hybridization to investigate Kunitz trypsin inhibitor gene expression programs at the cell level in soybean embryos and in transformed tobacco seeds. The major Kunitz trypsin...
A mutant lectin gene is rescued from an insertion element that blocks its expression.
The soybean lectin gene Le1 encodes a prevalent seed protein and is highly regulated during the life cycle. The mutant lectin gene allele le1 is not transcribed detectably, contains a 3.5-kb Tgm1...
Regional and cell-specific gene expression patterns during petal development.
Drews, G N, Beals, T P, Bui, A Q, Goldberg, R B
We investigated gene expression patterns that occur during tobacco petal development. Two petal mRNA classes were identified that are present at elevated levels relative to other organs. One class is...
Paradiso, M J, Roberts, G, Streicher, S L, Goldberg, R B
The viomycin phosphotransferase gene (vph) is expressed and confers resistance to viomycin in both Streptomyces spp. and members of the family Enterobacteriaceae. We report the isolation of UGA...
We have partially characterized the biochemical parameters of glutamine synthetase from Klebsiella pneumoniae and have shown that the differential affinity of adenylylated and unadenylylated...
Regulation of histidase synthesis in intergeneric hybrids of enteric bacteria.
Goldberg, R B, Bloom, F R, Magasanik, B
Regulation of the expression of the histidase coded by hutk of Klebsiella aerogenes in Salmonella typhimurium and in Escherichia coli and of the expression of the histidase coded by huts of S....
Transduction of chromosomal genes between enteric bacteria by bacteriophage P1.
We have used P1 transduction to create intergeneric hybrid strains of enteric bacteria by moving the genA and hut genes between Klebsiella aerogenes, Escherichia coli and Salmonella typhimurium. The...
Gene order of the histidine utilization (hut) operons in Klebsiella aerogenes.
P1-sensitive mutants of Klebsiella aerogenes were isolated and the gene order of the hut region was then determined using P1-mediated transduction. The genes are located in the Klebsiella chromosome...
Regulation of Klebsiella pneumoniae hut operons by oxygen.
We investigated the regulation of genes concerned with nitrogen metabolism by oxygen in the facultative anaerobe Klebsiella pneumoniae. We found oxygen to be required for the expression of the hut...
Regulation of nitrogen metabolism in glutamine auxotrophs of Klebsiella pneumoniae.
We examined the regulation of nitrogen metabolism in four classes (glnA, glnB, glnF, and glnG) of Gln- auxotrophs of Klebsiella pneumoniae. These studies indicate that glutamine synthetase does not...
Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group.
Cornelissen, B J, Horowitz, J, Van Kan, J A, Goldberg, R B, Bol, J F
Infection of Samsun NN tobacco with tobacco mosaic virus (TMV) was found to induce the synthesis of mRNA encoding a basic protein with a 67% amino acid sequence homology to the known acidic...
Organ-specific nuclear RNAs in tobacco.
We investigated the developmental regulation of nuclear RNA sequences in tobacco vegetative (leaf, root, stem) and floral (petal, ovary, anther) organ systems using RNA-excess X single-copy DNA...
Electron microscopy of Achlya deoxyribonucleic acid sequence organization.
Pellegrini, M, Timberlake, W E, Goldberg, R B
Electron microscopic analysis of reassociated deoxyribonucleic acid (DNA) from the aquatic fungus Achlya bisexualis revealed details of the sequence arrangement of the inverted repeats and both the...
Okamuro, J K, Jofuku, K D, Goldberg, R B
We introduced a 17.1-kilobase soybean DNA fragment containing the lectin gene and at least four nonseed protein genes into the tobacco genome. As in soybean plants, lectin mRNA is present in tobacco...
Female sterile tobacco plants are produced by stigma-specific cell ablation.
Goldman, M H, Goldberg, R B, Mariani, C
We identified a tobacco stigma-specific gene, designated STIG1. The STIG1 gene is developmentally regulated and expressed specifically in the stigmatic secretory zone. We used a chimeric STIG1-GUS...
Stacpoole, P W, Bridge, D M, Alvarez, I M, Goldberg, R B, Harwood, H J
3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoA reductase) controls the rate of cholesterol biosynthesis and is itself modulated through feedback suppression by internalized low density...
Joffe, B I, Goldberg, R B, Feinstein, J, Kark, A, Seftel, H C
Aspects of adipose tissue cellularity were examined in 15 non-diabetic premenopausal African women with simple obesity living in Johannesburg. A smaller group of six non-obese Black women served as...
Spondyloepiphyseal dysplasia congenita: genetic linkage to type II collagen (COL2AI).
Anderson, I J, Goldberg, R B, Marion, R W, Upholt, W B, Tsipouras, P
Spondyloepiphyseal dysplasia congenita (SEDC) is an autosomal dominantly inherited chondrodysplasia characterized by disproportionate short stature (short trunk), abnormal epiphyses, and flattened...