In vitro evaluation of tigemonam, a novel oral monobactam.
Tanaka, S K, Summerill, R A, Minassian, B F, Bush, K, Visnic, D A, Bonner, D P, ...
Tigemonam, a novel, orally administered monobactam, exhibited potent and specific activity in vitro against members of the family Enterobacteriaceae, Haemophilus influenzae, and Neisseria...
In vivo evaluation of tigemonam, a novel oral monobactam.
Clark, J M, Olsen, S J, Weinberg, D S, Dalvi, M, Whitney, R R, Bonner, D P, ...
Tigemonam, a new monobactam with excellent activity against gram-negative bacteria, was evaluated for in vivo efficacy and absorption after oral administration to laboratory animals. Tigemonam is...
Resistance caused by decreased penetration of beta-lactam antibiotics into Enterobacter cloacae.
Bush, K, Tanaka, S K, Bonner, D P, Sykes, R B
Strains of Enterobacter cloacae were selected on the basis of resistance to aztreonam, ceftazidime, moxalactam, or imipenem. All strains produced the same E2 beta-lactamase, with an isoelectric point...
Sykes, R B, Bonner, D P, Bush, K, Georgopapadakou, N H
Azthreonam (SQ 26,776) is a synthetic monocyclic beta-lactam antimicrobial agent belonging to the monobactam family (Sykes et al., Nature [London] 291:489-491, 1981), members of which are...
Biosynthesis of monobactam compounds: origin of the carbon atoms in the beta-lactam ring.
O'Sullivan, J, Gillum, A M, Aklonis, C A, Souser, M L, Sykes, R B
The biosynthesis of monobactams by strains of Chromobacterium violaceum, Acetobacter sp., and Agrobacterium radiobacter was studied. Monobactams were produced during logarithmic growth by C....
Georgopapadakou, N H, Smith, S A, Sykes, R B
Azthreonam (SQ 26,776) is a member of a new class of monocyclic beta-lactam antibiotics. In Escherichia coli, azthreonam caused filamentation at its lowest effective concentration (0.2 microgram/ml),...
Interaction of azthreonam and related monobactams with beta-lactamases from gram-negative bacteria.
Bush, K, Freudenberger, J S, Sykes, R B
Monobactams containing 3 beta-aminothiazolyl oxime side chains (SQ 81,377, SQ 81,402, azthreonam, and SQ 26,917) have poor affinities for the broad-spectrum beta-lactamases TEM-2 and K1. Addition of...
Georgopapadakou, N H, Smith, S A, Cimarusti, C M, Sykes, R B
A series of novel monocyclic beta-lactam antibiotics having side chains related to penicillin, piperacillin, azlocillin, and cefotaxime were examined with respect to binding to essential...
High-pressure liquid chromatographic analysis of aztreonam in sera and urine.
Pilkiewicz, F G, Remsburg, B J, Fisher, S M, Sykes, R B
Aztreonam (SQ 26,776) is a new synthetic monocyclic beta-lactam antibiotic which is specifically active against aerobic gram-negative bacteria. High-pressure liquid chromatographic (HPLC) systems...
Properties of the Beta-Lactamase Specified by the Pseudomonas Plasmid RPL11
Matthew, Margaret, Sykes, R. B.
The Pseudomonas plasmid RPL11 specifies a β-lactamase that has properties distinguishing it from other plasmid-mediated β-lactamases so far described, although it closely resembles plasmid Rms139...
Properties of an R Factor from Pseudomonas aeruginosa
Datta, Naomi, Hedges, R. W., Shaw, Elizabeth J., Sykes, R. B., Richmond, M. H.
An R factor from Pseudomonas aeruginosa, which confers resistance to penicillins, kanamycin, and tetracycline, was studied in Escherichia coli K-12. The R factor could coexist with F-like or I-like...
Properties of an R Factor Which Originated in Pseudomonas aeruginosa 1822
Grinsted, John, Saunders, J. R., Ingram, Lewis C., Sykes, R. B., Richmond, M. H.
RP1, a group of genes specifying resistance to carbenicillin, neomycin, kanamycin, and tetracycline and originating in a strain of Pseudomonas aeruginosa, was freely transmissible between strains of...
Newsom, S. W. B., Sykes, R. B., Richmond, M. H.
Examination of the substrate profile of a number of β-lactamases synthesized by strains of Pseudomonas aeruginosa has shown the presence of at least two distinct types of enzyme, one of which is...
Curtis, N. A. C., Richmond, M. H., Sykes, R. B.
Type IIIa β-lactamase (TEM β-lactamase) was “periplasmic” in Escherichia coli K-12 regardless of whether its gene was chromosomal or carried on an R factor.
Transfer of a Plasmid-Specified Beta-Lactamase Gene from Haemophilus influenzae
A number of ampicillin-resistant strains of Haemophilus influenzae could donate a gene specifying the type IIIa (TEM) β-lactamase to Haemophilus parainfluenzae, Escherichia coli, and Pseudomonas...
Comparative Activity of Ampicillin and Cefuroxime Against Three Types of Haemophilus influenzae
Sykes, R. B., Griffiths, A., Ryan, D. M.
On the basis of their susceptibility to ampicillin, strains of Haemophilus influenzae can be divided into three types: type 1 are normally susceptible strains, type 2 produce stable spheroplasts from...
Novel R-Plasmid-Mediated Beta-Lactamase from Klebsiella aerogenes
Petrocheilou, V., Sykes, R. B., Richmond, M. H.
A novel β-lactamase with a broad spectrum of activity against penicillins and cephalosporins has been detected in a strain of Klebsiella aerogenes. Its synthesis is mediated by an R-plasmid of...
Cefuroxime, a New Cephalosporin Antibiotic: Activity In Vitro
O'Callaghan, Cynthia H., Sykes, R. B., Griffiths, A., Thornton, J. E.
Cefuroxime is a new broad-spectrum cephalosporin antibiotic with increased stability to β-lactamases. This stability, although no absolute in all cases, has the effect of widening the antibacterial...
A New Cephalosporin with a Dual Mode of Action
O'Callaghan, Cynthia H., Sykes, R. B., Staniforth, Susan E.
A cephalosporin, (6R,7R)-7-[(2R)-2-hydroxy-2-phenylacetamido]-3-(pyrid-2-yl-N-oxide) thiomethylceph-3-em-4-carboxylic acid (MCO), that could lead to a novel approach to the problem of β-lactamase...
Microiodometric Determination of β-Lactamase Activity
The product of β-lactamase activity (a penicilloic acid in the case of a penicillin) is stoichiometrically oxidized by iodine. Hence, the β-lactamase activity can be measured as decolorization of...
Ingram, Lewis C., Richmond, M. H., Sykes, R. B.
An outbreak of R-factor-mediated carbenicillin resistance in Pseudomonas aeruginosa in burned patients in March 1969 was followed by a second outbreak 6 months later. No R-factor-carrying P....
In vitro evaluation of tigemonam, a novel oral monobactam.
Tanaka, S K, Summerill, R A, Minassian, B F, Bush, K, Visnic, D A, Bonner, D P, ...
Tigemonam, a novel, orally administered monobactam, exhibited potent and specific activity in vitro against members of the family Enterobacteriaceae, Haemophilus influenzae, and Neisseria...
In vivo evaluation of tigemonam, a novel oral monobactam.
Clark, J M, Olsen, S J, Weinberg, D S, Dalvi, M, Whitney, R R, Bonner, D P, ...
Tigemonam, a new monobactam with excellent activity against gram-negative bacteria, was evaluated for in vivo efficacy and absorption after oral administration to laboratory animals. Tigemonam is...
Resistance caused by decreased penetration of beta-lactam antibiotics into Enterobacter cloacae.
Bush, K, Tanaka, S K, Bonner, D P, Sykes, R B
Strains of Enterobacter cloacae were selected on the basis of resistance to aztreonam, ceftazidime, moxalactam, or imipenem. All strains produced the same E2 beta-lactamase, with an isoelectric point...
Sykes, R B, Bonner, D P, Bush, K, Georgopapadakou, N H
Azthreonam (SQ 26,776) is a synthetic monocyclic beta-lactam antimicrobial agent belonging to the monobactam family (Sykes et al., Nature [London] 291:489-491, 1981), members of which are...
Biosynthesis of monobactam compounds: origin of the carbon atoms in the beta-lactam ring.
O'Sullivan, J, Gillum, A M, Aklonis, C A, Souser, M L, Sykes, R B
The biosynthesis of monobactams by strains of Chromobacterium violaceum, Acetobacter sp., and Agrobacterium radiobacter was studied. Monobactams were produced during logarithmic growth by C....
Georgopapadakou, N H, Smith, S A, Sykes, R B
Azthreonam (SQ 26,776) is a member of a new class of monocyclic beta-lactam antibiotics. In Escherichia coli, azthreonam caused filamentation at its lowest effective concentration (0.2 microgram/ml),...
Interaction of azthreonam and related monobactams with beta-lactamases from gram-negative bacteria.
Bush, K, Freudenberger, J S, Sykes, R B
Monobactams containing 3 beta-aminothiazolyl oxime side chains (SQ 81,377, SQ 81,402, azthreonam, and SQ 26,917) have poor affinities for the broad-spectrum beta-lactamases TEM-2 and K1. Addition of...
Georgopapadakou, N H, Smith, S A, Cimarusti, C M, Sykes, R B
A series of novel monocyclic beta-lactam antibiotics having side chains related to penicillin, piperacillin, azlocillin, and cefotaxime were examined with respect to binding to essential...
High-pressure liquid chromatographic analysis of aztreonam in sera and urine.
Pilkiewicz, F G, Remsburg, B J, Fisher, S M, Sykes, R B
Aztreonam (SQ 26,776) is a new synthetic monocyclic beta-lactam antibiotic which is specifically active against aerobic gram-negative bacteria. High-pressure liquid chromatographic (HPLC) systems...
Properties of the Beta-Lactamase Specified by the Pseudomonas Plasmid RPL11
Matthew, Margaret, Sykes, R. B.
The Pseudomonas plasmid RPL11 specifies a β-lactamase that has properties distinguishing it from other plasmid-mediated β-lactamases so far described, although it closely resembles plasmid Rms139...
Properties of an R Factor from Pseudomonas aeruginosa
Datta, Naomi, Hedges, R. W., Shaw, Elizabeth J., Sykes, R. B., Richmond, M. H.
An R factor from Pseudomonas aeruginosa, which confers resistance to penicillins, kanamycin, and tetracycline, was studied in Escherichia coli K-12. The R factor could coexist with F-like or I-like...
Properties of an R Factor Which Originated in Pseudomonas aeruginosa 1822
Grinsted, John, Saunders, J. R., Ingram, Lewis C., Sykes, R. B., Richmond, M. H.
RP1, a group of genes specifying resistance to carbenicillin, neomycin, kanamycin, and tetracycline and originating in a strain of Pseudomonas aeruginosa, was freely transmissible between strains of...
Newsom, S. W. B., Sykes, R. B., Richmond, M. H.
Examination of the substrate profile of a number of β-lactamases synthesized by strains of Pseudomonas aeruginosa has shown the presence of at least two distinct types of enzyme, one of which is...
Curtis, N. A. C., Richmond, M. H., Sykes, R. B.
Type IIIa β-lactamase (TEM β-lactamase) was “periplasmic” in Escherichia coli K-12 regardless of whether its gene was chromosomal or carried on an R factor.
Transfer of a Plasmid-Specified Beta-Lactamase Gene from Haemophilus influenzae
A number of ampicillin-resistant strains of Haemophilus influenzae could donate a gene specifying the type IIIa (TEM) β-lactamase to Haemophilus parainfluenzae, Escherichia coli, and Pseudomonas...
Comparative Activity of Ampicillin and Cefuroxime Against Three Types of Haemophilus influenzae
Sykes, R. B., Griffiths, A., Ryan, D. M.
On the basis of their susceptibility to ampicillin, strains of Haemophilus influenzae can be divided into three types: type 1 are normally susceptible strains, type 2 produce stable spheroplasts from...
Novel R-Plasmid-Mediated Beta-Lactamase from Klebsiella aerogenes
Petrocheilou, V., Sykes, R. B., Richmond, M. H.
A novel β-lactamase with a broad spectrum of activity against penicillins and cephalosporins has been detected in a strain of Klebsiella aerogenes. Its synthesis is mediated by an R-plasmid of...
Cefuroxime, a New Cephalosporin Antibiotic: Activity In Vitro
O'Callaghan, Cynthia H., Sykes, R. B., Griffiths, A., Thornton, J. E.
Cefuroxime is a new broad-spectrum cephalosporin antibiotic with increased stability to β-lactamases. This stability, although no absolute in all cases, has the effect of widening the antibacterial...
A New Cephalosporin with a Dual Mode of Action
O'Callaghan, Cynthia H., Sykes, R. B., Staniforth, Susan E.
A cephalosporin, (6R,7R)-7-[(2R)-2-hydroxy-2-phenylacetamido]-3-(pyrid-2-yl-N-oxide) thiomethylceph-3-em-4-carboxylic acid (MCO), that could lead to a novel approach to the problem of β-lactamase...
Microiodometric Determination of β-Lactamase Activity
The product of β-lactamase activity (a penicilloic acid in the case of a penicillin) is stoichiometrically oxidized by iodine. Hence, the β-lactamase activity can be measured as decolorization of...
Ingram, Lewis C., Richmond, M. H., Sykes, R. B.
An outbreak of R-factor-mediated carbenicillin resistance in Pseudomonas aeruginosa in burned patients in March 1969 was followed by a second outbreak 6 months later. No R-factor-carrying P....