R. C. Miller

Publication List Details

Period

1968 - 2008

Number

106

Co-Authors

252 (2008)

R. C. Miller

for system printing, text, and graphics applications requiring flexibility and high print quality. Features such as their print density of 240 X 240 pelslin. ’ and all-point addressability extend...

Ovulation detection methods for urinary hormones: precision, daily and intermittent sampling and a combined hierarchical method (2006)

O’Connor, K.A., Brindle, E., Miller, R.C., Shofer, J.B., Ferrell, R.J., Klein, N.A., ...

BACKGROUND: We evaluate the performance of ovulation detection methods and present new approaches, including evaluation of methods for precision, combining multiple markers into a hierarchical system...

Ovulation detection methods for urinary hormones: precision, daily and intermittent sampling and a combined hierarchical method (2006)

O’Connor, K.A., Brindle, E., Miller, R.C., Shofer, J.B., Ferrell, R.J., Klein, N.A., ...

BACKGROUND: We evaluate the performance of ovulation detection methods and present new approaches, including evaluation of methods for precision, combining multiple markers into a hierarchical system...

Ovulation detection methods for urinary hormones: precision, daily and intermittent sampling and a combined hierarchical method (2006)

O’Connor, K.A., Brindle, E., Miller, R.C., Shofer, J.B., Ferrell, R.J., Klein, N.A., ...

BACKGROUND: We evaluate the performance of ovulation detection methods and present new approaches, including evaluation of methods for precision, combining multiple markers into a hierarchical system...

MAGNETIC AND ELECTRICAL ANOMALIES IN LACOO3, (2005)

HEIKES,R. R., Miller,R. C., Mazelsky,R.

The electrical conductivity and Seebeck coefficient of LaCoO3 drops rapidly with increasing temperature in the vicinity of 500K. The magnetic moment per cobalt atom also changes by about one Bohr...

Analysis of nitrogen and carbon tetrachloride adsorption isotherms and pore size distribution for siliceous MCM-41 synthesized from rice husk silica (2004)

Siriluk Chiarakorn, Nurak Grisdanurak, Miller, R. C.

RH-MCM-41 particles were synthesized using sodium silicate prepared from rice husk as a silica source and hexadecyltrimethylammonium bromide (CTAB) as a surfactant. The molar compositions were...

Analysis of nitrogen and carbon tetrachloride adsorption isotherms and pore size distribution for siliceous MCM-41 synthesized from rice husk silica (2004)

Siriluk Chiarakorn, Nurak Grisdanurak, Miller, R. C.

RH-MCM-41 particles were synthesized using sodium silicate prepared from rice husk as a silica source and hexadecyltrimethylammonium bromide (CTAB) as a surfactant. The molar compositions were...

Atlas of Oceanographic Information for Norton Sound, Alaska. (1998)

Ostrom, J. E., Comiskey, A. L., Miller, R. C.

This is a reference document on oceanography, meteorology, climate and ice conditions for the Norton Sound. The Norton Sound area is defined as the area between 160 degrees 30 min W to 170 degrees W,...

A Review, Evolution, and Correlation of the Phase Equilibria, Heat of Mixing, and Change in Volume on Mixing for Liquid Mixtures of Methane + Ethane, (1998)

Hiza, M. J., Miller, R. C., Kidnay, A. J.

The available experimental data for liquid-vapor equilibria, heat of mixing, and change in volume on mixing for the methane + ethane system have been reviewed and where possible evaluated for...

A Review, Evaluation, and Correlation of the Phase Equilibria, Heat of Mixing, and Change in Volume on Mixing for Liquid Mixtures of Methane + Propane, (1998)

Miller, R. C., Kidnay, A. J., Hiza, M. J.

The available experimental data for liquid-vapor equilibria, heat of mixing, and change in volume on mixing for the methane + propane system have been reviewed and where possible evaluated for...

Equilibrium Properties of Fluid Mixtures, A Bibliography of Data on Fluids of Cryogenic Interest, (1998)

Hiza, M. J., Kidnay, A. J., Miller, R. C.

This bibliography contains references to experimental phase equilibria and thermophysical property data for ten properties of fluid mixtures. The phase equilibria data considered are: solid-liquid,...

Biweekly List of Papers on Radiation Chemistry and Photochemistry. Annual Cumulation with Keyword and Author Indexes. Volume 15, 1982, (1998)

Hiza, M. J., Kidnay, A. J., Miller, R. C.

The Biweekly List of Papers on Radiation Chemistry and Photochemistry is a current-awareness service published by the Radiation Chemistry Data Center (RCDC), with special emphasis on the kinetics and...

Equilibrium Properties of Fluid Mixtures. 2. A Bibliography of Experimental Data on Selected Fluids. Update, (1998)

Hiza, M. J., Kidnay, A. J., Miller, R. C.

This bibliography updates to January 1980 an earlier bibliography on equilibrium properties of fluid mixtures of cryogenic interest, and is expanded to include pentane and aqueous mixtures. It...

Oncogenic Transformation Through the Cell Cycle and the LET Dependent Inverse Dose Rate Effect (1994)

Geard, C.R., Miller, R.C., Brenner, D.J., Hall, E.J.

Synchronised populations of mouse C3H/10T-1/2 cells were obtained by a stringent mitotic dislodgement procedure. Mitotic cells rapidly attach and progress sequentially through the cell cycle....

Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene (1993)

Graham, Roger W., Atkinson, Tom, Kilburn, D.G., Miller, R.C., Warren, R.A.J

A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Threelong oligonucleotides were synthesized and used in combination with flanking PCR...

Inverse Dose Rate Effects for Neutrons: General Features and Biophysical Consequences (1992)

Brenner, D.J., Miller, R.C., Marino, S.A., Geard, C.R., Randers-Pehrson, G., Hall, E.J.

Evidence has accumulated to suggest that when a neutron dose is protracted, its effectiveness is enhanced for transformational endpoints. A pattern has emerged as to the dependence of the effect on...

The Microdosimetric Link Between Oncogenic Transformation Data with Neutrons and Charged Particles (1990)

Hall, E.J., Brenner, D.J., Hei, T.K., Miller, R.C.

One of the most important challenges in biophysics is to predict the variation of biological effectiveness with radiation quality in terms of a physical quantity that can be measured or deduced from...

C1-Cx revisited: intramolecular synergism in a cellulase.

Din, N, Damude, H G, Gilkes, N R, Miller, R C, Warren, R A, Kilburn, D G

Endoglucanase A (CenA) from the bacterium Cellulomonas fimi is composed of a catalytic domain and a nonhydrolytic cellulose-binding domain that can function independently. The individual domains...

Comparison of a fungal (family I) and bacterial (family II) cellulose-binding domain.

Tomme, P, Driver, D P, Amandoron, E A, Miller, R C, Antony, R, Warren, J, ...

A family II cellulose-binding domain (CBD) of an exoglucanase/xylanase (Cex) from the bacterium Cellulomonas fimi was replaced with the family I CBD of cellobiohydrolase I (CbhI) from the fungus...

Purification and characterization of endoglucanase C of Cellulomonas fimi, cloning of the gene, and analysis of in vivo transcripts of the gene.

Moser, B, Gilkes, N R, Kilburn, D G, Warren, R A, Miller, R C

Two nonglycosylated endoglucanases which bind to Sephadex were purified from culture supernatants of Cellulomonas fimi grown on microcrystalline cellulose. Their Mrs were 120,000 and 130,000. The...

Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase.

Meinke, A, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A

Five cellulose-binding polypeptides were detected in Cellulomonas fimi culture supernatants. Two of them are CenA and CenB, endo-beta-1,4-glucanases which have been characterized previously; the...

Streptomyces lividans glycosylates the linker region of a beta-1,4-glycanase from Cellulomonas fimi.

Ong, E, Kilburn, D G, Miller, R C, Warren, R A

The beta-1,4-glycanase Cex of the gram-positive bacterium Cellulomonas fimi is a glycoprotein comprising a C-terminal cellulose-binding domain connected to an N-terminal catalytic domain by a linker...

Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi.

Meinke, A, Braun, C, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A

The nucleotide sequence of the cenB gene was determined and used to deduce the amino acid sequence of endoglucanase B (CenB) of Cellulomonas fimi. CenB comprises 1,012 amino acids and has a molecular...

Multiple domains in endoglucanase B (CenB) from Cellulomonas fimi: functions and relatedness to domains in other polypeptides.

Meinke, A, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A

Endoglucanase B (CenB) from the bacterium Cellulomonas fimi is divided into five discrete domains by linker sequences rich in proline and hydroxyamino acids (A. Meinke, C. Braun, N. R. Gilkes, D. G....

Structure and transcription analysis of the gene encoding a cellobiase from Agrobacterium sp. strain ATCC 21400.

Wakarchuk, W W, Greenberg, N M, Kilburn, D G, Miller, R C, Warren, R A

The DNA sequence was determined for the cloned Agrobacterium sp. strain ATCC 21400 beta-glucosidase gene, abg. High-resolution nuclease S1 protection studies were used to map the abg mRNA 5' and 3'...

Regulation, initiation, and termination of the cenA and cex transcripts of Cellulomonas fimi.

Greenberg, N M, Warren, R A, Kilburn, D G, Miller, R C

We characterized the in vivo transcripts of two Cellulomonas fimi genes, the cenA gene, which encodes an extracellular endo-beta-1,4-glucanase (EC 3.2.1.4) and the cex gene, which encodes an...

Regulation and initiation of cenB transcripts of Cellulomonas fimi.

Greenberg, N M, Warren, R A, Kilburn, D G, Miller, R C

We characterized the in vivo transcription of the Cellulomonas fimi cenB gene, which encodes an extracellular endo-beta-1,4-glucanase (EC 3.2.1.4). By Northern blot (RNA blot) analysis, cenB mRNA was...

Overproduction from a cellulase gene with a high guanosine-plus-cytosine content in Escherichia coli.

O'Neill, G P, Kilburn, D G, Warren, R A, Miller, R C

A recombinant exoglucanase was expressed in Escherichia coli to a level that exceeded 20% of total cellular protein. To obtain this level of overproduction, the exoglucanase gene coding sequence was...

Spontaneous temperature-sensitive mutations in bacteriophage T7.

Stone, J C, Miller, R C

Attempts to recover temperature-sensitive mutations affecting genes 13 and 14 (virion proteins) in bacteriophage T7 by analysis of amber revertants were confounded by the frequent occurrence of...

Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene.

Graham, R W, Atkinson, T, Kilburn, D G, Miller, R C, Warren, R A

A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking...

Genes for tRNALys5 from Drosophila melanogaster.

DeFranco, D, Burke, K B, Hayashi, S, Tener, G M, Miller, R C, Söll, D

The sequences of two cloned genes from Drosophila which hybridize with tRNALys5 are reported. One gene, in plasmid pDt39, has a sequence which corresponds to the sequence of tRNA. The other gene, in...

Transcription of cloned transfer RNA genes from Drosophila melanogaster in a homologous cell-free extract.

Rajput, B, Duncan, L, DeMille, D, Miller, R C, Spiegelman, G B

Cloned Drosophila melanogaster tRNA genes have been transcribed in a homologous cell-free extract isolated from a Schneider II cell line. The major product of the reaction is a tRNA precursor which...

Electron microscopic analysis of partially replicated bacteriophage T7 DNA.

Burck, K B, Scraba, D G, Miller, R C

Partially replicated bacteriophage T7 DNA was isolated from Escherichia coli infected with UV-irradiated T7 bacteriophage and was analyzed by electron microscopy. The analysis determined the...

T4 DNA polymerase (gene 43) is required in vivo for repair of gaps in recombinants.

Miller, R C

Experiments with a mutant of T4, tsL97, temperature sensitive for gene 43, showed that T4 DNA polymerase was necessary in vivo to repair gaps in recombinant molecules. CsCl density gradient...

Biological activity of T4 DNA synthesized in toluene-treated Escherichia coli cells.

McNicol, L A, Miller, R C

T4 DNA synthesized in a toluene-treated cell system can act as the genetic donor in a DNA transformation assay. This material transforms a variety of markers at high efficiency. We present evidence...

Alkali lability of bacteriophage phi W-14 DNA.

Lewis, H A, Miller, R C, Stone, J C, Warren, R A

The molecular weight of bacteriophage phi W-14 DNA, determined by velocity sedimentation in neutral sucrose gradients, was 92 +/- 6 X 10(6). The DNA showed marked fragmentation in alkaline sucrose...

Replication of T4 DNA in Escherichia coli Treated with Toluene

Miller, R. C., Taylor, D. M., Mackay, K., Smith, H. W.

Deoxyribonucleoside triphosphates are incorporated into T4 DNA in infected cells treated with toluene. Under the proper conditions the incorporation is controlled by the known T4 DNA polymerase and...

Small differences in Drosophila tropomyosin expression have significant effects on muscle function.

Tansey, T, Schultz, J R, Miller, R C, Storti, R V

The effects of promoter deletions on Drosophila tropomyosin I (TmI) gene expression have been determined by measuring TmI RNA levels in transformed flies. Decreases in RNA levels have been correlated...

Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.

Gilkes, N R, Henrissat, B, Kilburn, D G, Miller, R C, Warren, R A

Several types of domain occur in beta-1, 4-glycanases. The best characterized of these are the catalytic domains and the cellulose-binding domains. The domains may be joined by linker sequences rich...

Early Intracellular Events in the Replication of Bacteriophage T4 Deoxyribonucleic Acid: VI. Newly Synthesized Proteins in the T4 Protein-Deoxyribonucleic Acid Complex 1

Miller, R. C., Buckley, P.

Shortly after infection of Escherichia coli B with T4 phage, the phage deoxyribonucleic acid (DNA) can be isolated as a fast-sedimenting, proteinaceous complex. Formation of the complex is inhibited...

Marker rescue and partial replication of bacteriophage T7 DNA.

Burck, K B, Miller, R C

Experiments reported here show that some UV-irradiated wild-type T7 phage markers can be rescued efficiently by coinfection with T7 amber mutant phage in a permissive host. Other results show that...

Oncogenic transformation of mammalian cells in vitro with split doses of x-rays.

Miller, R C, Hall, E J, Rossi, H H

An established line of mouse fibroblasts, C3H/10T1/2 cells, was used for the assessment in vitro of oncogenic transformations caused by single and split doses of x-rays. The shape of the...

Folded, concatenated genomes as replication intermediates of bacteriophage T7 DNA.

Paetkau, V, Langman, L, Bradley, R, Scraba, D, Miller, R C

A complex form of bacteriophage T7 DNA, containing up to several hundred phage equivalents of DNA, arises during replication of T7. The complex was stable to treatment with ionic detergent, Pronase,...

Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi.

Shen, H, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A

The gene cbhB from the cellulolytic bacterium Cellulomonas fimi encodes a polypeptide of 1090 amino acids. Cellobiohydrolase B (CbhB) is 1037 amino acids long, with a calculated molecular mass of...

Changes in the molecular-size distribution of insoluble celluloses by the action of recombinant Cellulomonas fimi cellulases.

Kleman-Leyer, K M, Gilkes, N R, Miller, R C, Kirk, T K

Specific patterns of attacks of cotton, bacterial cellulose and bacterial microcrystalline cellulose (BMCC) by recombinant cellulases of Cellulomonas fimi were investigated. Molecular-size...

The tertiary structure of a bacterial cellulase determined by small-angle X-ray-scattering analysis.

Pilz, I, Schwarz, E, Kilburn, D G, Miller, R C, Warren, R A, Gilkes, N R

CenA from Cellulomonas fimi is a beta-1,4-endoglucanase that binds tightly to cellulose. X-ray-scattering analyses show that the enzyme is tadpole-shaped: the previously identified catalytic and...

A comparative study of the localization of wheat-germ agglutinin and its potential receptors in wheat grains.

Miller, R C, Bowles, D J

Wheat-germ agglutinin is located only in the embryo of a dry wheat seed and not in the endosperm tissue. This distribution remains unaltered for up to 96 h of germination and growth. The lectin is...

C1-Cx revisited: intramolecular synergism in a cellulase.

Din, N, Damude, H G, Gilkes, N R, Miller, R C, Warren, R A, Kilburn, D G

Endoglucanase A (CenA) from the bacterium Cellulomonas fimi is composed of a catalytic domain and a nonhydrolytic cellulose-binding domain that can function independently. The individual domains...

Comparison of a fungal (family I) and bacterial (family II) cellulose-binding domain.

Tomme, P, Driver, D P, Amandoron, E A, Miller, R C, Antony, R, Warren, J, ...

A family II cellulose-binding domain (CBD) of an exoglucanase/xylanase (Cex) from the bacterium Cellulomonas fimi was replaced with the family I CBD of cellobiohydrolase I (CbhI) from the fungus...

Purification and characterization of endoglucanase C of Cellulomonas fimi, cloning of the gene, and analysis of in vivo transcripts of the gene.

Moser, B, Gilkes, N R, Kilburn, D G, Warren, R A, Miller, R C

Two nonglycosylated endoglucanases which bind to Sephadex were purified from culture supernatants of Cellulomonas fimi grown on microcrystalline cellulose. Their Mrs were 120,000 and 130,000. The...

Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase.

Meinke, A, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A

Five cellulose-binding polypeptides were detected in Cellulomonas fimi culture supernatants. Two of them are CenA and CenB, endo-beta-1,4-glucanases which have been characterized previously; the...

Streptomyces lividans glycosylates the linker region of a beta-1,4-glycanase from Cellulomonas fimi.

Ong, E, Kilburn, D G, Miller, R C, Warren, R A

The beta-1,4-glycanase Cex of the gram-positive bacterium Cellulomonas fimi is a glycoprotein comprising a C-terminal cellulose-binding domain connected to an N-terminal catalytic domain by a linker...

Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi.

Meinke, A, Braun, C, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A

The nucleotide sequence of the cenB gene was determined and used to deduce the amino acid sequence of endoglucanase B (CenB) of Cellulomonas fimi. CenB comprises 1,012 amino acids and has a molecular...

Multiple domains in endoglucanase B (CenB) from Cellulomonas fimi: functions and relatedness to domains in other polypeptides.

Meinke, A, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A

Endoglucanase B (CenB) from the bacterium Cellulomonas fimi is divided into five discrete domains by linker sequences rich in proline and hydroxyamino acids (A. Meinke, C. Braun, N. R. Gilkes, D. G....

Structure and transcription analysis of the gene encoding a cellobiase from Agrobacterium sp. strain ATCC 21400.

Wakarchuk, W W, Greenberg, N M, Kilburn, D G, Miller, R C, Warren, R A

The DNA sequence was determined for the cloned Agrobacterium sp. strain ATCC 21400 beta-glucosidase gene, abg. High-resolution nuclease S1 protection studies were used to map the abg mRNA 5' and 3'...

Regulation, initiation, and termination of the cenA and cex transcripts of Cellulomonas fimi.

Greenberg, N M, Warren, R A, Kilburn, D G, Miller, R C

We characterized the in vivo transcripts of two Cellulomonas fimi genes, the cenA gene, which encodes an extracellular endo-beta-1,4-glucanase (EC 3.2.1.4) and the cex gene, which encodes an...

Regulation and initiation of cenB transcripts of Cellulomonas fimi.

Greenberg, N M, Warren, R A, Kilburn, D G, Miller, R C

We characterized the in vivo transcription of the Cellulomonas fimi cenB gene, which encodes an extracellular endo-beta-1,4-glucanase (EC 3.2.1.4). By Northern blot (RNA blot) analysis, cenB mRNA was...

Overproduction from a cellulase gene with a high guanosine-plus-cytosine content in Escherichia coli.

O'Neill, G P, Kilburn, D G, Warren, R A, Miller, R C

A recombinant exoglucanase was expressed in Escherichia coli to a level that exceeded 20% of total cellular protein. To obtain this level of overproduction, the exoglucanase gene coding sequence was...

Spontaneous temperature-sensitive mutations in bacteriophage T7.

Stone, J C, Miller, R C

Attempts to recover temperature-sensitive mutations affecting genes 13 and 14 (virion proteins) in bacteriophage T7 by analysis of amber revertants were confounded by the frequent occurrence of...

Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene.

Graham, R W, Atkinson, T, Kilburn, D G, Miller, R C, Warren, R A

A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking...

Genes for tRNALys5 from Drosophila melanogaster.

DeFranco, D, Burke, K B, Hayashi, S, Tener, G M, Miller, R C, Söll, D

The sequences of two cloned genes from Drosophila which hybridize with tRNALys5 are reported. One gene, in plasmid pDt39, has a sequence which corresponds to the sequence of tRNA. The other gene, in...

Transcription of cloned transfer RNA genes from Drosophila melanogaster in a homologous cell-free extract.

Rajput, B, Duncan, L, DeMille, D, Miller, R C, Spiegelman, G B

Cloned Drosophila melanogaster tRNA genes have been transcribed in a homologous cell-free extract isolated from a Schneider II cell line. The major product of the reaction is a tRNA precursor which...

Electron microscopic analysis of partially replicated bacteriophage T7 DNA.

Burck, K B, Scraba, D G, Miller, R C

Partially replicated bacteriophage T7 DNA was isolated from Escherichia coli infected with UV-irradiated T7 bacteriophage and was analyzed by electron microscopy. The analysis determined the...

T4 DNA polymerase (gene 43) is required in vivo for repair of gaps in recombinants.

Miller, R C

Experiments with a mutant of T4, tsL97, temperature sensitive for gene 43, showed that T4 DNA polymerase was necessary in vivo to repair gaps in recombinant molecules. CsCl density gradient...

Biological activity of T4 DNA synthesized in toluene-treated Escherichia coli cells.

McNicol, L A, Miller, R C

T4 DNA synthesized in a toluene-treated cell system can act as the genetic donor in a DNA transformation assay. This material transforms a variety of markers at high efficiency. We present evidence...

Alkali lability of bacteriophage phi W-14 DNA.

Lewis, H A, Miller, R C, Stone, J C, Warren, R A

The molecular weight of bacteriophage phi W-14 DNA, determined by velocity sedimentation in neutral sucrose gradients, was 92 +/- 6 X 10(6). The DNA showed marked fragmentation in alkaline sucrose...

Replication of T4 DNA in Escherichia coli Treated with Toluene

Miller, R. C., Taylor, D. M., Mackay, K., Smith, H. W.

Deoxyribonucleoside triphosphates are incorporated into T4 DNA in infected cells treated with toluene. Under the proper conditions the incorporation is controlled by the known T4 DNA polymerase and...

Small differences in Drosophila tropomyosin expression have significant effects on muscle function.

Tansey, T, Schultz, J R, Miller, R C, Storti, R V

The effects of promoter deletions on Drosophila tropomyosin I (TmI) gene expression have been determined by measuring TmI RNA levels in transformed flies. Decreases in RNA levels have been correlated...

Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.

Gilkes, N R, Henrissat, B, Kilburn, D G, Miller, R C, Warren, R A

Several types of domain occur in beta-1, 4-glycanases. The best characterized of these are the catalytic domains and the cellulose-binding domains. The domains may be joined by linker sequences rich...

Early Intracellular Events in the Replication of Bacteriophage T4 Deoxyribonucleic Acid: VI. Newly Synthesized Proteins in the T4 Protein-Deoxyribonucleic Acid Complex 1

Miller, R. C., Buckley, P.

Shortly after infection of Escherichia coli B with T4 phage, the phage deoxyribonucleic acid (DNA) can be isolated as a fast-sedimenting, proteinaceous complex. Formation of the complex is inhibited...

Marker rescue and partial replication of bacteriophage T7 DNA.

Burck, K B, Miller, R C

Experiments reported here show that some UV-irradiated wild-type T7 phage markers can be rescued efficiently by coinfection with T7 amber mutant phage in a permissive host. Other results show that...

Oncogenic transformation of mammalian cells in vitro with split doses of x-rays.

Miller, R C, Hall, E J, Rossi, H H

An established line of mouse fibroblasts, C3H/10T1/2 cells, was used for the assessment in vitro of oncogenic transformations caused by single and split doses of x-rays. The shape of the...

Folded, concatenated genomes as replication intermediates of bacteriophage T7 DNA.

Paetkau, V, Langman, L, Bradley, R, Scraba, D, Miller, R C

A complex form of bacteriophage T7 DNA, containing up to several hundred phage equivalents of DNA, arises during replication of T7. The complex was stable to treatment with ionic detergent, Pronase,...

Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi.

Shen, H, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A

The gene cbhB from the cellulolytic bacterium Cellulomonas fimi encodes a polypeptide of 1090 amino acids. Cellobiohydrolase B (CbhB) is 1037 amino acids long, with a calculated molecular mass of...

Changes in the molecular-size distribution of insoluble celluloses by the action of recombinant Cellulomonas fimi cellulases.

Kleman-Leyer, K M, Gilkes, N R, Miller, R C, Kirk, T K

Specific patterns of attacks of cotton, bacterial cellulose and bacterial microcrystalline cellulose (BMCC) by recombinant cellulases of Cellulomonas fimi were investigated. Molecular-size...

The tertiary structure of a bacterial cellulase determined by small-angle X-ray-scattering analysis.

Pilz, I, Schwarz, E, Kilburn, D G, Miller, R C, Warren, R A, Gilkes, N R

CenA from Cellulomonas fimi is a beta-1,4-endoglucanase that binds tightly to cellulose. X-ray-scattering analyses show that the enzyme is tadpole-shaped: the previously identified catalytic and...

A comparative study of the localization of wheat-germ agglutinin and its potential receptors in wheat grains.

Miller, R C, Bowles, D J

Wheat-germ agglutinin is located only in the embryo of a dry wheat seed and not in the endosperm tissue. This distribution remains unaltered for up to 96 h of germination and growth. The lectin is...

Catecholamine uptake by isolated coronary arteries and atria of the kitten.

Cornish, E J, Goldie, R G, Miller, R C

Kitten coronary arteries and atria incubated with [3H]-(-)-noradrenaline or [3H]-(+/-)-isoprenaline showed concentration- and temperature-dependent accumulation of amines (unchanged and/or as...

Effect of endothelium on basal and on stimulated accumulation and efflux of cyclic GMP in rat isolated aorta.

Schini, V., Schoeffter, P., Miller, R. C.

1. The aim of this study was to examine the possible role of the release of guanosine 3':5'-cyclic monophosphate (cyclic GMP) into the extracellular space in the regulation of rat aortic cyclic GMP...

Effects of calcium entry blockers on contractions evoked by endothelin-1, [Ala3,11]endothelin-1 and [Ala1,15]endothelin-1 in rat isolated aorta.

Topouzis, S., Pelton, J. T., Miller, R. C.

1. The aim of the present study was to examine the contractile responses evoked by the recently characterized vasoactive peptide endothelin-1 (ET-1) and by two of its structural analogues,...

Insensitivity of calcium-dependent endothelial stimulation in rat isolated aorta to the calcium entry blocker, flunarizine.

Miller, R. C., Schoeffter, P., Stoclet, J. C.

In rat aortic segments complete with endothelium, acetylcholine (1 microM) relaxed noradrenaline, phenylephrine and prostaglandin F2 alpha (PGF2 alpha)-induced contractions of various magnitudes....

Modulation by endothelium of contractile responses in rat aorta in absence and presence of flunarizine.

Miller, R. C., Stoclet, J. C.

The possible modulation by endothelium of phenylephrine- and prostaglandin F2 alpha-induced mobilization of calcium for contraction in the rat aorta has been investigated. Contractions elicited by...

Binding of [125I]-endothelin-1 to rat cerebellar homogenates and its interactions with some analogues.

Hiley, C. R., Jones, C. R., Pelton, J. T., Miller, R. C.

1. [125I]-endothelin-1, over the concentration range 6 pM-10 nM, bound to a single site in homogenates of rat cerebellum with high affinity (Kd = 2.8 +/- 0.6 x 10(-10) M). The site was present in a...

Modulation by endothelium of the responses induced by endothelin-1 and by some of its analogues in rat isolated aorta.

Topouzis, S., Huggins, J. P., Pelton, J. T., Miller, R. C.

1. The contractile effects of endothelin-1 and various analogues were studied in rat isolated rings. The potency of the peptides studied was endothelin-1 greater than sarafotoxin S6b greater than...

Enhanced responsiveness of rat isolated aorta to clonidine after removal of the endothelial cells.

Eglème, C., Godfraind, T., Miller, R. C.

With the endothelium present, the maximum response of rat isolated aorta to clonidine was much lower than that to noradrenaline. Removal of endothelium enhanced the response to both adrenoceptor...

Endothelial mediated inhibition of contraction and increase in cyclic GMP levels evoked by the alpha-adrenoceptor agonist B-HT 920 in rat isolated aorta.

Miller, R. C., Mony, M., Schini, V., Schoeffter, P., Stoclet, J. C.

In the presence of endothelium maximal contractions of rat aorta preparations evoked by B-HT 920 were about 10% of those evoked in the absence of endothelium....

Effects of uptake inhibitors on responses of sheep coronary arteries to catecholamines and sympathetic nerve stimulation.

Brine, F., Cornish, E. J., Miller, R. C.

1. Transmural stimulation of intrinsic sympathetic nerves and exogenous catecholamines produce beta 1-adrenoceptor mediated relaxant responses in strips of contracted sheep coronary artery. 2. The...

Selective alpha 1- and alpha 2-adrenoceptor agonist-induced contractions and 45Ca fluxes in the rat isolated aorta.

Godfraind, T., Miller, R. C., Lima, J. S.

1 Contractile responses produced by the alpha 1-adrenoceptor selective agonist, phenylephrine, and the alpha 2-adrenoceptor selective agonists, oxymetazoline and clonidine, have been compared to...

Effects of histamine and the histamine antagonists mepyramine and cimetidine on human coronary arteries in vitro.

Godfraind, T., Miller, R. C.

The effects of histamine have been studied on human isolated coronary artery preparations taken from hearts ranging in age from 9 to 73 years. Histamine in large concentrations (100 microM)...

Effects of yohimbine, rauwolscine and corynanthine on contractions and calcium fluxes induced by depolarization and prostaglandin F2 alpha in rat aorta.

Godfraind, T., Miller, R. C., Socrates Lima, J.

The effects of the selective alpha 2-adrenoceptor antagonists yohimbine and its stereo-isomer rauwolscine and the selective alpha 1-adrenoceptor antagonist corynanthine (a third yohimbine...

Actions of prostaglandin F2 alpha and noradrenaline on calcium exchange and contraction in rat mesenteric arteries and their sensitivity to calcium entry blockers.

Godfraind, T., Miller, R. C.

1 The actions of prostaglandin F2 alpha (PGF2 alpha) and noradrenaline on contraction and 45Ca exchange have been studied in rat mesenteric arteries. 2 PGF2 alpha and noradrenaline contracted rat...

Vasoconstrictor effects of various neuropeptide Y analogues on the rat tail artery in the presence of phenylephrine.

Tschöpl, M., Miller, R. C., Pelton, J., Stoclet, J. C., Bucher, B.

1. The increase in perfusion pressure induced by neuropeptide Y (NPY), peptide YY (PYY) and related peptides were compared in the perfused rat tail artery precontracted by a submaximal concentration...

Oncogenic transformation in vitro by the hypoxic cell sensitizer misonidazole.

Miller, R. C., Hall, E. J.

The hypoxic cell sensitizer Misonidazole (Ro-07-0582) induces oncogenic transformations in the C3H/10T1/2 mouse embryo cell line cultured in vitro. A drug concentration of 0.4 mM applied to aerated...

The oncogenic potential of a combination of hyperthermia and chemotherapy agents.

Komatsu, K., Miller, R. C., Hall, E. J.

The modulating effect of 43 degrees C hyperthermia on the induction of oncogenic transformation by the antineoplastic agents, actinomycin D, mitomycin C, and 1,3-bis(2-chloroethyl)-1-nitrosourea...