The lacrimal gland secretes most of the water and many proteins present in tear fluid. The composition of the tear fluid is affected dramatically by androgens, an observation which has been linked to...
Masson, V, Gerard, R D, Schmitt, P M, Albert, V, Praus, M, ...
The plasminogen (Plg)/plasminogen activator (PA) system plays a key role in cancer progression, presumably via mediating extracellular matrix degradation and tumor cell migration. Consequently,...
Bajou, Khalid, Masson, Véronique, Gerard, R D, Schmitt, P M, Albert, V, Praus, M, ...
The plasminogen (Plg)/plasminogen activator (PA) system plays a key role in cancer progression, presumably via mediating extracellular matrix degradation and tumor cell migration. Consequently,...
Gillijns, H, Iung, B, Meurrens, K, ...
Local intracoronary delivery of recombinant adenoviruses expressing anti-migratory or anti-proliferative proteins including human constitutive endothelial nitric oxide synthase (NOS3), plasminogen...
Recombinant adenoviral (Ad) vectors represent an efficient gene transfer system for targeting the cardiovascular system. Phenotypic modulation of coronary vascular cells in vivo is, however,...
Pislaru, S, Van Pelt, N, Gerard, R D, Zoldhelyi, P, ...
BACKGROUND: Nitric oxide, synthesized from L-arginine by nitric oxide synthase (NOS), is a vasodilator and inhibits vascular smooth muscle cell (SMC) proliferation and migration. The effects of local...
Noël, A, Gerard, R D, Masson, V, Brunner, N, Holst-Hansen, C, ...
Acquisition of invasive/metastatic potential through protease expression is an essential event in tumor progression. High levels of components of the plasminogen activation system, including...
Bajou, Khalid, Noël, Agnès, Gerard, R D, Masson, Véronique, Brunner, N, Holst-Hansen, C, ...
Acquisition of invasive/metastatic potential through protease expression is an essential event in tumor progression. High levels of components of the plasminogen activation system, including...
Janssens, S, Lupu, F, ...
BACKGROUND: Plasminogen-deficient mice display impaired vascular wound healing and reduced arterial neointima formation after arterial injury, suggesting that inhibition of plasmin generation might...
Van Vlaenderen, I, Meidell, R S, Gerard, R D
Impaired fibrinolysis, resulting from increased plasminogen activator inhibitor-1 (PAI-1) or reduced tissue-type plasminogen activator (t-PA) plasma levels, may predispose the individual to subacute...
Bloch, K D, Nong, Z, Gerard, R D, Zoldhelyi, P
Nitric oxide (NO), a vasodilator involved in the regulation of pulmonary vascular tone, is synthesized by a family of enzymes, nitric oxide synthases (NOS). To investigate whether adenoviral-mediated...
Varley, A W, Coulthard, M G, Meidell, R S, Gerard, R D, Munford, R S
We report that promoters for two murine acute-phase protein (APP) genes, complement factor 3 (C3) and serum amyloid A3 (SAA3), can increase recombinant protein expression in response to inflammatory...
We have explored the use of adenovirus-mediated gene transfer to transiently elicit production of low density lipoprotein (LDL) receptors in mice. A recombinant adenovirus carrying the human LDL...
Madison, E L, Goldsmith, E J, Gerard, R D, Gething, M J, Sambrook, J F, Bassel-Duby, R S
Fibrinolysis is regulated in part by the interaction between tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1, a serine protease inhibitor of the serpin family)....
Coulthard, M G, Swindle, J, Munford, R S, Gerard, R D, Meidell, R S
Although the host response to gram-negative bacterial infection follows largely from the interactions of bacterial lipopolysaccharides (LPS or endotoxin) with host cells, little information is...
Henry, L J, Xia, D, Wilke, M E, Deisenhofer, J, Gerard, R D
The adenovirus fiber protein is used for attachment of the virus to a specific receptor on the cell surface. Structurally, the protein consists of a long, thin shaft that protrudes from the vertex of...
Analysis of nonpermissivity in mouse cells overexpressing simian virus 40 T antigen.
Gerard, R D, Guggenheimer, R A, Gluzman, Y
To analyze the nature of the nonpermissivity of mouse cells for simian virus 40 (SV40) DNA replication, we isolated mouse cells producing SV40 T antigen (Tag) at levels equal to or greater than that...
Ishibashi, S, Brown, M S, Goldstein, J L, Gerard, R D, Hammer, R E, Herz, J
We employed homologous recombination in embryonic stem cells to produce mice lacking functional LDL receptor genes. Homozygous male and female mice lacking LDL receptors (LDLR-/- mice) were viable...
Mohr, I J, Gluzman, Y, Fairman, M P, Strauss, M, McVey, D, Stillman, B, ...
A bacterial expression system was used to produce simian virus 40 large tumor antigen (T antigen) in the absence of the extensive posttranslational modifications that occur in mammalian cells....
Role of specific simian virus 40 sequences in the nuclease-sensitive structure in viral chromatin.
Gerard, R D, Montelone, B A, Walter, C F, Innis, J W, Scott, W A
A nuclease-sensitive region forms in chromatin containing a 273-base-pair (bp) segment of simian virus 40 DNA encompassing the viral origin of replication and early and late promoters. We have...
New host cell system for regulated simian virus 40 DNA replication.
Transformed monkey cell lines (CMT and BMT) that inducible express simian virus 40 (SV40) T antigen from the metallothionein promoter have been isolated and characterized. Immunoprecipitation of...
Deletion mutants which affect the nuclease-sensitive site in simian virus 40 chromatin.
Gerard, R D, Woodworth-Gutai, M, Scott, W A
A short segment of simian virus 40 (SV40) chromatin on the late side of the origin of replication is hypersensitive to nuclease cleavage. The role of DNA sequence information in this...
Gething, M J, Adler, B, Boose, J A, Gerard, R D, Madison, E L, McGookey, D, ...
The heavy chain of tissue plasminogen activator (t-PA) consists of four domains [finger, epidermal-growth-factor (EGF)-like, kringle 1 and kringle 2] that are homologous to similar domains present in...
Janssens, S P, Bloch, K D, Nong, Z, Gerard, R D, Zoldhelyi, P, Collen, D
Nitric oxide (NO), a vasodilator involved in the regulation of pulmonary vascular tone, is synthesized by a family of enzymes, nitric oxide synthases (NOS). To investigate whether adenoviral-mediated...
Systemic hypertension induced by hepatic overexpression of human preproendothelin-1 in rats.
Niranjan, V, Télémaque, S, DeWit, D, Gerard, R D, Yanagisawa, M
Endothelin-1 (ET-1) has been implicated in the regulation of vascular tone in various pathological conditions. To examine the effect of in vivo overexpression of the peptide in rats, we prepared...
T antigen and template requirements for SV40 DNA replication in vitro.
Stillman, B, Gerard, R D, Guggenheimer, R A, Gluzman, Y
A cell-free system for replication of SV40 DNA was used to assess the effect of mutations altering either the SV40 origin of DNA replication or the virus-encoded large tumor (T) antigen. Plasmid DNAs...
Baqué, S, Newgard, C B, Gerard, R D, Guinovart, J J, Gómez-Foix, A M
The feasibility of using adenovirus as a vector for the introduction of glycogen phosphorylase activity into myocytes has been examined. We used the C2C12 myoblast cell line to assay the impact of...
Varley, A W, Coulthard, M G, Meidell, R S, Gerard, R D, Munford, R S
We report that promoters for two murine acute-phase protein (APP) genes, complement factor 3 (C3) and serum amyloid A3 (SAA3), can increase recombinant protein expression in response to inflammatory...
We have explored the use of adenovirus-mediated gene transfer to transiently elicit production of low density lipoprotein (LDL) receptors in mice. A recombinant adenovirus carrying the human LDL...
Madison, E L, Goldsmith, E J, Gerard, R D, Gething, M J, Sambrook, J F, Bassel-Duby, R S
Fibrinolysis is regulated in part by the interaction between tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1, a serine protease inhibitor of the serpin family)....
Coulthard, M G, Swindle, J, Munford, R S, Gerard, R D, Meidell, R S
Although the host response to gram-negative bacterial infection follows largely from the interactions of bacterial lipopolysaccharides (LPS or endotoxin) with host cells, little information is...
Henry, L J, Xia, D, Wilke, M E, Deisenhofer, J, Gerard, R D
The adenovirus fiber protein is used for attachment of the virus to a specific receptor on the cell surface. Structurally, the protein consists of a long, thin shaft that protrudes from the vertex of...
Analysis of nonpermissivity in mouse cells overexpressing simian virus 40 T antigen.
Gerard, R D, Guggenheimer, R A, Gluzman, Y
To analyze the nature of the nonpermissivity of mouse cells for simian virus 40 (SV40) DNA replication, we isolated mouse cells producing SV40 T antigen (Tag) at levels equal to or greater than that...
Ishibashi, S, Brown, M S, Goldstein, J L, Gerard, R D, Hammer, R E, Herz, J
We employed homologous recombination in embryonic stem cells to produce mice lacking functional LDL receptor genes. Homozygous male and female mice lacking LDL receptors (LDLR-/- mice) were viable...
Mohr, I J, Gluzman, Y, Fairman, M P, Strauss, M, McVey, D, Stillman, B, ...
A bacterial expression system was used to produce simian virus 40 large tumor antigen (T antigen) in the absence of the extensive posttranslational modifications that occur in mammalian cells....
Role of specific simian virus 40 sequences in the nuclease-sensitive structure in viral chromatin.
Gerard, R D, Montelone, B A, Walter, C F, Innis, J W, Scott, W A
A nuclease-sensitive region forms in chromatin containing a 273-base-pair (bp) segment of simian virus 40 DNA encompassing the viral origin of replication and early and late promoters. We have...
New host cell system for regulated simian virus 40 DNA replication.
Transformed monkey cell lines (CMT and BMT) that inducible express simian virus 40 (SV40) T antigen from the metallothionein promoter have been isolated and characterized. Immunoprecipitation of...
Deletion mutants which affect the nuclease-sensitive site in simian virus 40 chromatin.
Gerard, R D, Woodworth-Gutai, M, Scott, W A
A short segment of simian virus 40 (SV40) chromatin on the late side of the origin of replication is hypersensitive to nuclease cleavage. The role of DNA sequence information in this...
Gething, M J, Adler, B, Boose, J A, Gerard, R D, Madison, E L, McGookey, D, ...
The heavy chain of tissue plasminogen activator (t-PA) consists of four domains [finger, epidermal-growth-factor (EGF)-like, kringle 1 and kringle 2] that are homologous to similar domains present in...
Janssens, S P, Bloch, K D, Nong, Z, Gerard, R D, Zoldhelyi, P, Collen, D
Nitric oxide (NO), a vasodilator involved in the regulation of pulmonary vascular tone, is synthesized by a family of enzymes, nitric oxide synthases (NOS). To investigate whether adenoviral-mediated...
Systemic hypertension induced by hepatic overexpression of human preproendothelin-1 in rats.
Niranjan, V, Télémaque, S, DeWit, D, Gerard, R D, Yanagisawa, M
Endothelin-1 (ET-1) has been implicated in the regulation of vascular tone in various pathological conditions. To examine the effect of in vivo overexpression of the peptide in rats, we prepared...
T antigen and template requirements for SV40 DNA replication in vitro.
Stillman, B, Gerard, R D, Guggenheimer, R A, Gluzman, Y
A cell-free system for replication of SV40 DNA was used to assess the effect of mutations altering either the SV40 origin of DNA replication or the virus-encoded large tumor (T) antigen. Plasmid DNAs...
Baqué, S, Newgard, C B, Gerard, R D, Guinovart, J J, Gómez-Foix, A M
The feasibility of using adenovirus as a vector for the introduction of glycogen phosphorylase activity into myocytes has been examined. We used the C2C12 myoblast cell line to assay the impact of...