Wakamiya, M, Blackburn, M R, Jurecic, R, McArthur, M J, Geske, R S, Cartwright, J, ...
We have generated mice with a null mutation at the Ada locus, which encodes the purine catabolic enzyme adenosine deaminase (ADA, EC 3.5.4.4). ADA-deficient fetuses exhibited hepatocellular...
Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells.
Kaufman, R J, Murtha, P, Ingolia, D E, Yeung, C Y, Kellems, R E
We demonstrate that an adenosine deaminase (ADA) cDNA gene can function as a dominant selectable and amplifiable marker for gene transfer experiments in mammalian cells. Cells that incorporate the...
Structural features of the transcription termination region for the mouse dihydrofolate reductase gene have been determined and compared with those of several other known termination regions for...
Kash, S F, Innis, J W, Jackson, A U, Kellems, R E
Transcription arrest plays a role in regulating the expression of a number of genes, including the murine adenosine deaminase (ADA) gene. We have previously identified two prominent arrest sites at...
Chen, Z, Harless, M L, Wright, D A, Kellems, R E
Analysis of human adenosine deaminase (ADA) gene transcription in four different cell lines indicated that a high density of RNA polymerase II complexes is present at the 5' end of the gene and that...
An elongation block to RNA polymerase II transcription in exon 1 is a major regulatory step in expression of the murine adenosine deaminase (ADA) gene. Previous work in the laboratory identified...
Sequence requirements for transcriptional arrest in exon 1 of the human adenosine deaminase gene.
Chen, Z, Innis, J W, Sun, M H, Wright, D A, Kellems, R E
We have previously demonstrated that a transcriptional arrest site exists in exon 1 of the human adenosine deaminase (ADA) gene and that this site may play a role in ADA gene expression (Z. Chen, M....
Sequence requirements for transcriptional arrest in exon 1 of the murine adenosine deaminase gene.
Ramamurthy, V, Maa, M C, Harless, M L, Wright, D A, Kellems, R E
We have previously shown that a transcription arrest site near the 5' end of the murine adenosine deaminase (ADA) gene is significantly involved in the regulation of ADA gene expression. To...
Linton, J P, Yen, J Y, Selby, E, Chen, Z, Chinsky, J M, Liu, K, ...
The mouse dihydrofolate reductase gene (dhfr) is a housekeeping gene expressed under the control of a promoter region embedded in a CpG island--a region rich in unmethylated CpG dinucleotides. A...
Analysis of the mouse dhfr promoter region: existence of a divergently transcribed gene.
Crouse, G F, Leys, E J, McEwan, R N, Frayne, E G, Kellems, R E
The use of murine dihydrofolate reductase (dhfr) gene amplification mutants enabled us to identify important structural and functional features of the dhfr promoter region. We found another...
Ingolia, D E, Al-Ubaidi, M R, Yeung, C Y, Bigo, H A, Wright, D, Kellems, R E
A genomic library was prepared with DNA from a genetically enriched mouse cell line in which amplified copies of the adenosine deaminase (ADA) gene account for over 5% of the genome. Overlapping...
Independent 5' and 3'-end determination of multiple dihydrofolate reductase transcripts.
Multiple dihydrofolate reductase (dhfr) mRNAs, differing substantially in abundance, are produced as a result of the utilization of multiple transcription initiation sites and multiple...
Yoder, S S, Robberson, B L, Leys, E J, Hook, A G, Al-Ubaidi, M, Yeung, C Y, ...
Infection of human cells by adenovirus results in multiple alterations of host gene expression. To examine the effects of viral infection on the expression of a single gene, a line of human cells was...
Frayne, E G, Leys, E J, Crouse, G F, Hook, A G, Kellems, R E
We observed equimolar transcription throughout the 35-kilobase mouse dihydrofolate reductase structural gene. Transcription termination occurred within a discrete region (900 base pairs) located 1...
Control of dihydrofolate reductase messenger ribonucleic acid production.
We used methotrexate-resistant mouse cells in which dihydrofolate reductase levels are approximately 500 times normal to study the effect of growth stimulation on dihydrofolate reductase gene...
Control of transcription arrest in intron 1 of the murine adenosine deaminase gene.
Transcription arrest plays a key role in the regulation of the murine adenosine deaminase (ADA) gene, as well as a number of other cellular and viral genes. We have previously characterized the ADA...
Wakamiya, M, Blackburn, M R, Jurecic, R, McArthur, M J, Geske, R S, Cartwright, J, ...
We have generated mice with a null mutation at the Ada locus, which encodes the purine catabolic enzyme adenosine deaminase (ADA, EC 3.5.4.4). ADA-deficient fetuses exhibited hepatocellular...
Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells.
Kaufman, R J, Murtha, P, Ingolia, D E, Yeung, C Y, Kellems, R E
We demonstrate that an adenosine deaminase (ADA) cDNA gene can function as a dominant selectable and amplifiable marker for gene transfer experiments in mammalian cells. Cells that incorporate the...
Structural features of the transcription termination region for the mouse dihydrofolate reductase gene have been determined and compared with those of several other known termination regions for...
Control of transcription arrest in intron 1 of the murine adenosine deaminase gene.
Transcription arrest plays a key role in the regulation of the murine adenosine deaminase (ADA) gene, as well as a number of other cellular and viral genes. We have previously characterized the ADA...
Kash, S F, Innis, J W, Jackson, A U, Kellems, R E
Transcription arrest plays a role in regulating the expression of a number of genes, including the murine adenosine deaminase (ADA) gene. We have previously identified two prominent arrest sites at...
Chen, Z, Harless, M L, Wright, D A, Kellems, R E
Analysis of human adenosine deaminase (ADA) gene transcription in four different cell lines indicated that a high density of RNA polymerase II complexes is present at the 5' end of the gene and that...
An elongation block to RNA polymerase II transcription in exon 1 is a major regulatory step in expression of the murine adenosine deaminase (ADA) gene. Previous work in the laboratory identified...
Sequence requirements for transcriptional arrest in exon 1 of the human adenosine deaminase gene.
Chen, Z, Innis, J W, Sun, M H, Wright, D A, Kellems, R E
We have previously demonstrated that a transcriptional arrest site exists in exon 1 of the human adenosine deaminase (ADA) gene and that this site may play a role in ADA gene expression (Z. Chen, M....
Sequence requirements for transcriptional arrest in exon 1 of the murine adenosine deaminase gene.
Ramamurthy, V, Maa, M C, Harless, M L, Wright, D A, Kellems, R E
We have previously shown that a transcription arrest site near the 5' end of the murine adenosine deaminase (ADA) gene is significantly involved in the regulation of ADA gene expression. To...
Linton, J P, Yen, J Y, Selby, E, Chen, Z, Chinsky, J M, Liu, K, ...
The mouse dihydrofolate reductase gene (dhfr) is a housekeeping gene expressed under the control of a promoter region embedded in a CpG island--a region rich in unmethylated CpG dinucleotides. A...
Analysis of the mouse dhfr promoter region: existence of a divergently transcribed gene.
Crouse, G F, Leys, E J, McEwan, R N, Frayne, E G, Kellems, R E
The use of murine dihydrofolate reductase (dhfr) gene amplification mutants enabled us to identify important structural and functional features of the dhfr promoter region. We found another...
Ingolia, D E, Al-Ubaidi, M R, Yeung, C Y, Bigo, H A, Wright, D, Kellems, R E
A genomic library was prepared with DNA from a genetically enriched mouse cell line in which amplified copies of the adenosine deaminase (ADA) gene account for over 5% of the genome. Overlapping...
Independent 5' and 3'-end determination of multiple dihydrofolate reductase transcripts.
Multiple dihydrofolate reductase (dhfr) mRNAs, differing substantially in abundance, are produced as a result of the utilization of multiple transcription initiation sites and multiple...
Yoder, S S, Robberson, B L, Leys, E J, Hook, A G, Al-Ubaidi, M, Yeung, C Y, ...
Infection of human cells by adenovirus results in multiple alterations of host gene expression. To examine the effects of viral infection on the expression of a single gene, a line of human cells was...
Frayne, E G, Leys, E J, Crouse, G F, Hook, A G, Kellems, R E
We observed equimolar transcription throughout the 35-kilobase mouse dihydrofolate reductase structural gene. Transcription termination occurred within a discrete region (900 base pairs) located 1...
Control of dihydrofolate reductase messenger ribonucleic acid production.
We used methotrexate-resistant mouse cells in which dihydrofolate reductase levels are approximately 500 times normal to study the effect of growth stimulation on dihydrofolate reductase gene...