Delay, E.R., Hernandez, N.P., Bromley, K., Margolskee, R.F.
Molecular and behavioral studies have identified heterodimers of the T1R family as receptors for detecting the tastes of sweet (T1R2 + T1R3) and umami (T1R1 + T1R3). However, behavioral studies have...
Delay, E.R., Hernandez, N.P., Bromley, K., Margolskee, R.F.
Molecular and behavioral studies have identified heterodimers of the T1R family as receptors for detecting the tastes of sweet (T1R2 + T1R3) and umami (T1R1 + T1R3). However, behavioral studies have...
Molecular Mechanisms of Sweet Receptor Function (2005)
Jiang, P., Cui, M., Ji, Q., Snyder, L., Liu, Z., Benard, L., ...
Phototransduction in transgenic mice after targeted deletion of the rod transducin α-subunit
Calvert, P. D., Krasnoperova, N. V., Lyubarsky, A. L., Isayama, T., Nicoló, M., Kosaras, B., ...
Retinal photoreceptors use the heterotrimeric G protein transducin to couple rhodopsin to a biochemical cascade that underlies the electrical photoresponse. Several isoforms of each transducin...
Ausubel, F M, Bird, S C, Durbin, K J, Janssen, K A, Margolskee, R F, Peskin, A P
Previous studies have implicated glutamine synthetase (L-glutamate:ammonia ligase [adenosine diphosphate for-ing], EC 6.6.1.2) as a major controlling element of the nitrogen fixation (nif) genes in...
Penna, A, Fowler, P, Bertoletti, A, Guilhot, S, Moss, B, Margolskee, R F, ...
In this study, we show that CD4+, hepatitis B virus (HBV) envelope-specific T-cell clones produced by stimulation with a particulate antigen preparation are able to recognize and kill not only...
Guilhot, S, Fowler, P, Portillo, G, Margolskee, R F, Ferrari, C, Bertoletti, A, ...
To analyze the hepatitis B virus (HBV)-specific cytotoxic T-cell (CTL) response during acute and chronic viral hepatitis, target cells that express HBV-encoded antigens in the context of the...
We isolated second-site revertants of a partially defective VP1 mutant of simian virus 40. The suppressing mutation in each of these pseudorevertants was mapped to the viral agnogene. Of six...
Base substitution of the ori region of simian virus 40 leads to plaque morphology mutants with markedly decreased DNA replication. Second-site mutations within the simian virus 40 T antigen gene...
Spickofsky, N, Margolskee, R F
A new procedure using an asymmetrically tailed linker-primer plasmid has been developed to prepare extremely high complexity cDNA libraries. This procedure yields plasmid primed libraries with a...
Canfield, V, Emanuel, J R, Spickofsky, N, Levenson, R, Margolskee, R F
Site-directed mutagenesis was used to identify residues responsible for the greater than 1,000-fold difference in ouabain sensitivity between the rat Na,K-ATPase alpha 1 and alpha 2 isoforms. A...
Margolskee, R F, Kavathas, P, Berg, P
Efficient transfection and expression of cDNA libraries in human cells has been achieved with an Epstein-Barr virus-based subcloning vector (EBO-pcD). The plasmid vector contains a resistance marker...
Shortle, D R, Margolskee, R F, Nathans, D
The circular genome of simian virus 40 is a model mammalian replicon, containing a unique origin of replication (ori) and coding for a protein (SV40 T antigen) known to be involved in initiation of...
Functional expression of the taste specific G-protein, alpha-gustducin.
Hoon, M A, Northup, J K, Margolskee, R F, Ryba, N J
The taste-specific G-protein alpha-subunit, alpha-gustducin, was expressed using a baculovirus based system. alpha-Gustducin was demonstrated to be myristoylated and was also palmitoylated in insect...
Phototransduction in transgenic mice after targeted deletion of the rod transducin α-subunit
Calvert, P. D., Krasnoperova, N. V., Lyubarsky, A. L., Isayama, T., Nicoló, M., Kosaras, B., ...
Retinal photoreceptors use the heterotrimeric G protein transducin to couple rhodopsin to a biochemical cascade that underlies the electrical photoresponse. Several isoforms of each transducin...
Ausubel, F M, Bird, S C, Durbin, K J, Janssen, K A, Margolskee, R F, Peskin, A P
Previous studies have implicated glutamine synthetase (L-glutamate:ammonia ligase [adenosine diphosphate for-ing], EC 6.6.1.2) as a major controlling element of the nitrogen fixation (nif) genes in...
Penna, A, Fowler, P, Bertoletti, A, Guilhot, S, Moss, B, Margolskee, R F, ...
In this study, we show that CD4+, hepatitis B virus (HBV) envelope-specific T-cell clones produced by stimulation with a particulate antigen preparation are able to recognize and kill not only...
Guilhot, S, Fowler, P, Portillo, G, Margolskee, R F, Ferrari, C, Bertoletti, A, ...
To analyze the hepatitis B virus (HBV)-specific cytotoxic T-cell (CTL) response during acute and chronic viral hepatitis, target cells that express HBV-encoded antigens in the context of the...
We isolated second-site revertants of a partially defective VP1 mutant of simian virus 40. The suppressing mutation in each of these pseudorevertants was mapped to the viral agnogene. Of six...
Base substitution of the ori region of simian virus 40 leads to plaque morphology mutants with markedly decreased DNA replication. Second-site mutations within the simian virus 40 T antigen gene...
Spickofsky, N, Margolskee, R F
A new procedure using an asymmetrically tailed linker-primer plasmid has been developed to prepare extremely high complexity cDNA libraries. This procedure yields plasmid primed libraries with a...
Canfield, V, Emanuel, J R, Spickofsky, N, Levenson, R, Margolskee, R F
Site-directed mutagenesis was used to identify residues responsible for the greater than 1,000-fold difference in ouabain sensitivity between the rat Na,K-ATPase alpha 1 and alpha 2 isoforms. A...
Margolskee, R F, Kavathas, P, Berg, P
Efficient transfection and expression of cDNA libraries in human cells has been achieved with an Epstein-Barr virus-based subcloning vector (EBO-pcD). The plasmid vector contains a resistance marker...
Shortle, D R, Margolskee, R F, Nathans, D
The circular genome of simian virus 40 is a model mammalian replicon, containing a unique origin of replication (ori) and coding for a protein (SV40 T antigen) known to be involved in initiation of...
Functional expression of the taste specific G-protein, alpha-gustducin.
Hoon, M A, Northup, J K, Margolskee, R F, Ryba, N J
The taste-specific G-protein alpha-subunit, alpha-gustducin, was expressed using a baculovirus based system. alpha-Gustducin was demonstrated to be myristoylated and was also palmitoylated in insect...