Wallace, R. Bruce, Shaffer, J., Murphy, R. F., Bonner, J., Hirose, T., Itakura, K.
Oligodeoxyribonucleotides complementary to the DNA of the wild type (wt) bacteriophage ΦX174 have been synthesized by the phosphotriester method. The ollgomers, 11, 14, and 17 bases long, are...
Gonadotropin-releasing hormone analogue conjugates with strong selective antitumor activity
Pályi, I., Vincze, B., Lovas, S., Mező, I., Pató, J., Kálnay, A., ...
Conjugation of gonadotropin-releasing hormone (GnRH) analogues GnRH-III, MI-1544, and MI-1892 through lysyl side chains and a tetrapeptide spacer, Gly-Phe-Leu-Gly (X) to a copolymer,...
Bioavailability of oral trimetrexate in patients with acquired immunodeficiency syndrome.
Rogers, P, Allegra, C J, Murphy, R F, Drake, J C, Masur, H, Poplack, D G, ...
The combination of the lipophilic antifolate trimetrexate and the rescue agent leucovorin has shown promise in the treatment of Pneumocystis carinii pneumonia in patients with acquired...
Regulation of endocytic pH by the Na+,K+-ATPase in living cells.
Cain, C C, Sipe, D M, Murphy, R F
Acidification of endocytosed ligands destined for lysosomes is biphasic, with a rapid drop to pH 6, followed by a slow decrease to pH 5. Continuous measurements of transferrin acidification have...
Assay of vacuolar pH in yeast and identification of acidification-defective mutants.
Preston, R A, Murphy, R F, Jones, E W
As part of a genetic analysis of the biogenesis and function of the vacuole (lysosome) in the yeast Saccharomyces cerevisiae, assays of vacuolar pH were developed and used to identify mutants...
The kinetics of acidification of diferric human transferrin in BALB/c mouse 3T3 cells were determined by flow cytometry using a modification of the fluorescein-rhodamine fluorescence ratio technique....
Wallace, R B, Shaffer, J, Murphy, R F, Bonner, J, Hirose, T, Itakura, K
Oligodeoxyribonucleotides complementary to the DNA of the wild type (wt) bacteriophage phi chi 174 have been synthesized by the phosphotriester method. The oligomers, 11, 14, and 17 bases long, are...
Murphy, R F, Pearson, W R, Bonner, J
Computer programs for the analysis of data from techniques frequently used in nucleic acids research are described. In addition to calculating non-linear, least-squares solutions to equations...
Isolation of newly replicated chromatin by using shallow metrizamide gradients.
Murphy, R F, Wallace, R B, Bonner, J
The properties of chromatin containing newly synthesized DNA and protein were investigated. A fraction of chromatin enriched in newly replicated DNA was isolated by means of its increased density in...
Structure of transcriptionally active chromatin.
Gottesfeld, J M, Murphy, R F, Bonner, J
Rat-liver chromatin has bee fractionated into transcriptionally active and inactive regions [Gottesfeld et al. (1974) Proc. Nat. Acad. Sci. USA 71, 2193-2197] and the distribution of...
The existence of three distinct classes of endocytic vesicles that are part of the pathway of fluid-phase endocytosis has been demonstrated by flow cytometry. Amounts of fluorescent and scattered...
Altered nucleosome spacing in newly replicated chromatin from Friend leukemia cells.
Murphy, R F, Wallace, R B, Bonner, J
Chromatin from Friend leukemia cells labeled with [14C]thymidine for 24 hr followed by [3H]thymidine for 10 min is converted into nucleosomes by staphylococcal nuclease at only half the rate that...
Physical properties of chemically acetylated rat liver chromatin.
Wallace, R B, Sargent, T D, Murphy, R F, Bonner, J
The physical properties of rat liver chromatin and nucleosomes acetylated with acetic anhydride were examined in order to clarify the mechanism by which chemical acetylation of histones increases...
The native forms of the phycobilin chromophores of algal biliproteins. A clarification.
O'Carra, P, Murphy, R F, Killilea, S D
Pigments released from phycoerythrins and phycocyanins by treatment with hot methanol are currently regarded as equivalent to the native chromophores phycoerythrobilin and phycocyanobilin. However,...
Theoretical considerations on the role of membrane potential in the regulation of endosomal pH.
Rybak, S L, Lanni, F, Murphy, R F
Na+,K(+)-ATPase has been observed to partially inhibit acidification of early endosomes by increasing membrane potential, whereas chloride channels have been observed to enhance acidification in...
Toward objective selection of representative microscope images.
Markey, M K, Boland, M V, Murphy, R F
Scientists wishing to communicate the essential characteristics of a pattern (such as an immunofluorescence distribution) currently must make a subjective choice of one or two images to publish. We...
Release of secretin immunoreactivity from the ileum.
Tranberg, K G, Tabata, K, Chen, M H, Murphy, R F, Fischer, J E, Joffe, S N
In the rat, about 80% of the intestinal content of secretin immunoreactivity is found distal to the duodenum with a peak in the ileum. This work therefore studied whether the secretin...
Gimmon, Z, Murphy, R F, Chen, M H, Nachbauer, C A, Fischer, J E, Joffe, S N
To compare the effect of parenteral with enteral nutrition on gastroentero-pancreatic hormones, hypercaloric and hypocaloric nutrient preparations, commonly used clinically, were administered to rats...
Cyclic changes of plasma pancreatic polypeptide and pancreatic secretion in fasting dogs.
Chen, M H, Joffe, S N, Magee, D F, Murphy, R F, Naruse, S
Fasting conscious dogs, each with a gastric fistula, Heidenhain pouch and Thomas duodenal fistula, were used. Basal pancreatic secretion showed periodic increases in phase with the periodic...
Dental health of Louisiana residents based on the ten-state nutrition survey.
Morgan, P M, Murphy, R F, Willis, R A, Hubbard, D W, Norton, J M
The dental health status of 4,006 residents of Louisiana was analyzed, based on data in the 1968-70 Ten-State Nutrition Survey funded by the U.S. Government. These data were based on examinations of...
Gonadotropin-releasing hormone analogue conjugates with strong selective antitumor activity
Pályi, I., Vincze, B., Lovas, S., Mező, I., Pató, J., Kálnay, A., ...
Conjugation of gonadotropin-releasing hormone (GnRH) analogues GnRH-III, MI-1544, and MI-1892 through lysyl side chains and a tetrapeptide spacer, Gly-Phe-Leu-Gly (X) to a copolymer,...
Bioavailability of oral trimetrexate in patients with acquired immunodeficiency syndrome.
Rogers, P, Allegra, C J, Murphy, R F, Drake, J C, Masur, H, Poplack, D G, ...
The combination of the lipophilic antifolate trimetrexate and the rescue agent leucovorin has shown promise in the treatment of Pneumocystis carinii pneumonia in patients with acquired...
Regulation of endocytic pH by the Na+,K+-ATPase in living cells.
Cain, C C, Sipe, D M, Murphy, R F
Acidification of endocytosed ligands destined for lysosomes is biphasic, with a rapid drop to pH 6, followed by a slow decrease to pH 5. Continuous measurements of transferrin acidification have...
Assay of vacuolar pH in yeast and identification of acidification-defective mutants.
Preston, R A, Murphy, R F, Jones, E W
As part of a genetic analysis of the biogenesis and function of the vacuole (lysosome) in the yeast Saccharomyces cerevisiae, assays of vacuolar pH were developed and used to identify mutants...
The kinetics of acidification of diferric human transferrin in BALB/c mouse 3T3 cells were determined by flow cytometry using a modification of the fluorescein-rhodamine fluorescence ratio technique....
Wallace, R B, Shaffer, J, Murphy, R F, Bonner, J, Hirose, T, Itakura, K
Oligodeoxyribonucleotides complementary to the DNA of the wild type (wt) bacteriophage phi chi 174 have been synthesized by the phosphotriester method. The oligomers, 11, 14, and 17 bases long, are...
Murphy, R F, Pearson, W R, Bonner, J
Computer programs for the analysis of data from techniques frequently used in nucleic acids research are described. In addition to calculating non-linear, least-squares solutions to equations...
Isolation of newly replicated chromatin by using shallow metrizamide gradients.
Murphy, R F, Wallace, R B, Bonner, J
The properties of chromatin containing newly synthesized DNA and protein were investigated. A fraction of chromatin enriched in newly replicated DNA was isolated by means of its increased density in...
Structure of transcriptionally active chromatin.
Gottesfeld, J M, Murphy, R F, Bonner, J
Rat-liver chromatin has bee fractionated into transcriptionally active and inactive regions [Gottesfeld et al. (1974) Proc. Nat. Acad. Sci. USA 71, 2193-2197] and the distribution of...
The existence of three distinct classes of endocytic vesicles that are part of the pathway of fluid-phase endocytosis has been demonstrated by flow cytometry. Amounts of fluorescent and scattered...
Altered nucleosome spacing in newly replicated chromatin from Friend leukemia cells.
Murphy, R F, Wallace, R B, Bonner, J
Chromatin from Friend leukemia cells labeled with [14C]thymidine for 24 hr followed by [3H]thymidine for 10 min is converted into nucleosomes by staphylococcal nuclease at only half the rate that...
Physical properties of chemically acetylated rat liver chromatin.
Wallace, R B, Sargent, T D, Murphy, R F, Bonner, J
The physical properties of rat liver chromatin and nucleosomes acetylated with acetic anhydride were examined in order to clarify the mechanism by which chemical acetylation of histones increases...
The native forms of the phycobilin chromophores of algal biliproteins. A clarification.
O'Carra, P, Murphy, R F, Killilea, S D
Pigments released from phycoerythrins and phycocyanins by treatment with hot methanol are currently regarded as equivalent to the native chromophores phycoerythrobilin and phycocyanobilin. However,...
Theoretical considerations on the role of membrane potential in the regulation of endosomal pH.
Rybak, S L, Lanni, F, Murphy, R F
Na+,K(+)-ATPase has been observed to partially inhibit acidification of early endosomes by increasing membrane potential, whereas chloride channels have been observed to enhance acidification in...
Cyclic changes of plasma pancreatic polypeptide and pancreatic secretion in fasting dogs.
Chen, M H, Joffe, S N, Magee, D F, Murphy, R F, Naruse, S
Fasting conscious dogs, each with a gastric fistula, Heidenhain pouch and Thomas duodenal fistula, were used. Basal pancreatic secretion showed periodic increases in phase with the periodic...
Release of secretin immunoreactivity from the ileum.
Tranberg, K G, Tabata, K, Chen, M H, Murphy, R F, Fischer, J E, Joffe, S N
In the rat, about 80% of the intestinal content of secretin immunoreactivity is found distal to the duodenum with a peak in the ileum. This work therefore studied whether the secretin...
Toward objective selection of representative microscope images.
Markey, M K, Boland, M V, Murphy, R F
Scientists wishing to communicate the essential characteristics of a pattern (such as an immunofluorescence distribution) currently must make a subjective choice of one or two images to publish. We...
Gimmon, Z, Murphy, R F, Chen, M H, Nachbauer, C A, Fischer, J E, Joffe, S N
To compare the effect of parenteral with enteral nutrition on gastroentero-pancreatic hormones, hypercaloric and hypocaloric nutrient preparations, commonly used clinically, were administered to rats...
Dental health of Louisiana residents based on the ten-state nutrition survey.
Morgan, P M, Murphy, R F, Willis, R A, Hubbard, D W, Norton, J M
The dental health status of 4,006 residents of Louisiana was analyzed, based on data in the 1968-70 Ten-State Nutrition Survey funded by the U.S. Government. These data were based on examinations of...