R. G. Brennan

Secondary structure creates mismatched base pairs required for high-affinity binding of cAMP response element-binding protein to the human enkephalin enhancer.

Spiro, C, Richards, J P, Chandrasekaran, S, Brennan, R G, McMurray, C T

Transactivation studies of the enkephalin enhancer indicate that two cAMP response elements (CRE-1 and CRE-2) are needed to mediate the transcriptional response to cAMP and to the CRE-binding protein...

Engineered leucine zippers show that hemiphosphorylated CREB complexes are transcriptionally active.

Loriaux, M M, Rehfuss, R P, Brennan, R G, Goodman, R H

The ability of basic/leucine zipper transcription factors to form homo- and heterodimers potentially increases the diversity of signaling pathways that can impinge upon a single genetic element. The...

Escherichia coli biotin holoenzyme synthetase/bio repressor crystal structure delineates the biotin- and DNA-binding domains.

Wilson, K P, Shewchuk, L M, Brennan, R G, Otsuka, A J, Matthews, B W

The three-dimensional structure of BirA, the repressor of the Escherichia coli biotin biosynthetic operon, has been determined by x-ray crystallography and refined to a crystallographic residual of...

Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex.

Brennan, R G, Roderick, S L, Takeda, Y, Matthews, B W

The structure of a complex of bacteriophage lambda Cro protein with a 17-base-pair operator has been determined at 3.9-A resolution. Isomorphous derivatives obtained by the synthesis of site-specific...

X-ray structure of cytidine-5'-O-dimethylphosphate. Novel stacking between the ribosyl O(2') hydroxyl oxygen atom and the base.

Brennan, R G, Kondo, N S, Sundaralingam, M

The anionic oxygen atoms of the phosphodiester backbone of RNA and DNA are particularly susceptible to esterification by many mutagenic and carcinogenic alkylating agents. To better understand the...

Crystal structures of Toxoplasma gondii uracil phosphoribosyltransferase reveal the atomic basis of pyrimidine discrimination and prodrug binding.

Schumacher, M A, Carter, D, Scott, D M, Roos, D S, Ullman, B, Brennan, R G

Uracil phosphoribosyltransferase (UPRTase) catalyzes the transfer of a ribosyl phosphate group from alpha-D-5-phosphoribosyl-1-pyrophosphate to the N1 nitrogen of uracil. The UPRTase from the...

Crystal structures of adenine phosphoribosyltransferase from Leishmania donovani.

Phillips, C L, Ullman, B, Brennan, R G, Hill, C P

The enzyme adenine phosphoribosyltransferase (APRT) functions to salvage adenine by converting it to adenosine-5-monophosphate (AMP). APRT deficiency in humans is a well characterized inborn error of...

Secondary structure creates mismatched base pairs required for high-affinity binding of cAMP response element-binding protein to the human enkephalin enhancer.

Spiro, C, Richards, J P, Chandrasekaran, S, Brennan, R G, McMurray, C T

Transactivation studies of the enkephalin enhancer indicate that two cAMP response elements (CRE-1 and CRE-2) are needed to mediate the transcriptional response to cAMP and to the CRE-binding protein...

Engineered leucine zippers show that hemiphosphorylated CREB complexes are transcriptionally active.

Loriaux, M M, Rehfuss, R P, Brennan, R G, Goodman, R H

The ability of basic/leucine zipper transcription factors to form homo- and heterodimers potentially increases the diversity of signaling pathways that can impinge upon a single genetic element. The...

Escherichia coli biotin holoenzyme synthetase/bio repressor crystal structure delineates the biotin- and DNA-binding domains.

Wilson, K P, Shewchuk, L M, Brennan, R G, Otsuka, A J, Matthews, B W

The three-dimensional structure of BirA, the repressor of the Escherichia coli biotin biosynthetic operon, has been determined by x-ray crystallography and refined to a crystallographic residual of...

Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex.

Brennan, R G, Roderick, S L, Takeda, Y, Matthews, B W

The structure of a complex of bacteriophage lambda Cro protein with a 17-base-pair operator has been determined at 3.9-A resolution. Isomorphous derivatives obtained by the synthesis of site-specific...

X-ray structure of cytidine-5'-O-dimethylphosphate. Novel stacking between the ribosyl O(2') hydroxyl oxygen atom and the base.

Brennan, R G, Kondo, N S, Sundaralingam, M

The anionic oxygen atoms of the phosphodiester backbone of RNA and DNA are particularly susceptible to esterification by many mutagenic and carcinogenic alkylating agents. To better understand the...

Crystal structures of Toxoplasma gondii uracil phosphoribosyltransferase reveal the atomic basis of pyrimidine discrimination and prodrug binding.

Schumacher, M A, Carter, D, Scott, D M, Roos, D S, Ullman, B, Brennan, R G

Uracil phosphoribosyltransferase (UPRTase) catalyzes the transfer of a ribosyl phosphate group from alpha-D-5-phosphoribosyl-1-pyrophosphate to the N1 nitrogen of uracil. The UPRTase from the...

Crystal structures of adenine phosphoribosyltransferase from Leishmania donovani.

Phillips, C L, Ullman, B, Brennan, R G, Hill, C P

The enzyme adenine phosphoribosyltransferase (APRT) functions to salvage adenine by converting it to adenosine-5-monophosphate (AMP). APRT deficiency in humans is a well characterized inborn error of...

Preliminary structural studies on the multi-ligand-binding domain of the transcription activator, BmrR, from Bacillus subtilis.

Zheleznova, E. E., Markham, P. N., Neyfakh, A. A., Brennan, R. G.

In the bacterium Bacillus subtilis, the DNA-binding regulatory protein, BmrR, activates transcription from the multidrug transporter gene, bmr, after binding either rhodamine or...