Caveolin-1 is required for lateral line neuromast and notochord development (2007)
Nixon, S. J., Carter, A., Wegner, J., Ferguson, C., Floetenmeyer, M., Riches, J. D., ...
Caveolae have been linked to diverse cellular functions and to many disease states. In this study we have used zebrafish to examine the role of caveolin-1 and caveolae during early embryonic...
The multiple faces of caveolae (2007)
Caveolae are a highly abundant but enigmatic feature of mammalian cells. They form remarkably stable membrane domains at the plasma membrane but can also function as carriers in the exocytic and...
Plasma membrane nanoswitches generate high-fidelity Ras signal transduction (2007)
Tian, T. H., Harding, A., Inder, K., Plowman, S., Parton, R. G., Hancock, J. F.
Ras proteins occupy dynamic plasma membrane nanodomains called nanoclusters. The significance of this spatial organization is unknown. Here we show, using in silico and in vivo analyses of...
Chadda, R., Mark H. T., Plowman, S. J., Hancock, J. F., Parton, R. G., Mayor, S.
Glycosyl-phosphatidylinositol (GPI)-anchored proteins (GPI-APs) are present at the surface of living cells in cholesterol dependent nanoscale clusters. These clusters appear to act as sorting signals...
Reassessing the Role of Phosphocaveolin-1 in Cell Adhesion and Migration (2007)
Hill, M. M., Scherbakov, N., Schiefermeier, N., Baran, J., Hancock, J. F., Huber, L. A., ...
Although phosphorylation on tyrosine 14 was identified early in the discovery of caveolin-1, the functional significance of this modification still remains elusive. Recent evidence points to a role...
Lipid droplets: a unified view of a dynamic organelle (2006)
Lipid droplets form the main lipid store in eukaryotic cells. Although all cells seem to be able to generate lipid droplets, their biogenesis, regulatory mechanisms and interactions with other...
Ras plasma membrane signaling platforms (2006)
Plowman, S., Harding, A. S., Tinhai, T., Parton, R. G., Hancock, J. F.
Caveolin-1 is essential for liver regeneration (2006)
Fernandez, M. A., Albor, C., Ingelmo-Torres, M., Nixon, S. J., Ferguson, C., Kurzchalia, T., ...
Liver regeneration is an orchestrated cellular response that coordinates cell activation, lipid metabolism, and cell division. We found that caveolin-1 gene - disrupted mice (cav1(-/-) mice)...
Le Lay, S., Hajduch, E., Lindsay, M. R., Le Liepvre, X., Thiele, C., Ferre, P., ...
We have investigated the targeting of caveolin to lipid bodies in adipocytes that express high levels of caveolins and contain well-developed lipid droplets. We observed that the lipid droplets...
Dynamic microtubules regulate the local concentration of E-cadherin at cell-cell contacts (2006)
Stehbens, S. J., Paterson, A. D., Crampton, M. S., Shewan, A. M., Ferguson, C., Akhmanova, A., ...
In contrast to the well-established relationship between cadherins and the actin cytoskeleton, the potential link between cadherins and microtubules (MTs) has been less extensively investigated. We...
Trushina, E., Singh, R. P., Dyer, B., Cao, S., Shah, H., Parton, R. G., ...
We show that the mutant Huntington's disease (HD) protein (mhtt) specifically inhibits endocytosis in primary striatal neurons. Unexpectedly, mhtt does not inhibit clathrin-dependent endocytosis as...
Ultrastructural identification of uncoated caveolin-independent early endocytic vehicles (2005)
Kirkham, M., Fujita, A., Chadda, R., Nixon, S. J., Kurzchalia, T. V., Sharma, D. K., ...
Nicolau, D. V., Burrage, K., Parton, R. G., Hancock, J. F.
The dynamic lateral segregation of signaling proteins into microdomains is proposed to facilitate signal transduction, but the constraints on microdomain size, mobility, and diffusion that might...
Plowman, S. J., Muncke, C., Parton, R. G., Hancock, J. F.
Plasma membrane compartmentalization imposes lateral segregation on membrane proteins that is important for regulating signal transduction. We use computational modeling of immunogold spatial point...
Ras plasma membrane signalling platforms (2005)
The plasma membrane is a complex, dynamic structure that provides platforms for the assembly of many signal transduction pathways. These platforms have the capacity to impose an additional level of...
Caveolin, cholesterol, and lipid bodies (2005)
In mammalian cells a complex interplay regulates the distribution of cholesterol between intracellular membrane compartments. One important aspect of cholesterol regulation is intracellular...
Ultrastructural identification of uncoated caveolin-independent early endocytic vehicles (2005)
Kirkham, M., Fujita, A., Chadda, R., Nixon, S. J., Kurzchalia, T. V., Sharma, D. K., ...
Using quantitative light microscopy and a modified immunoelectron microscopic technique, we have characterized the entry pathway of the cholera toxin binding subunit (CTB) in primary embryonic...
Nixon, S. J., Wegner, J., Ferguson, C., Mery, P. F., Hancock, J. F., Currie, P. D., ...
Caveolae are an abundant feature of many animal cells. However, the exact function of caveolae remains unclear. We have used the zebrafish, Danio rerio, as a system to understand caveolae function...
Martin, S., Driessen, K., Nixon, S. J., Zerial, M., Parton, R. G.
Rab GTPases are crucial regulators of membrane traffic. Here we have examined a possible association of Rab proteins with lipid droplets (LDs), neutral lipid-containing organelles surrounded by a...
Pol, A., Martin, S., Fernandez, M. A., Ingelmo-Torres, M., Ferguson, C., Enrich, C., ...
Caveolins are a crucial component of plasma membrane (PM) caveolae but have also been localized to intracellular compartments, including the Golgi complex and lipid bodies. Mutant caveolins...
Characterisation of Rab23, a negative reegulator of sonic hedgehog signaling (2005)
Evans, T. M., Simpson-Fraser, F., Parton, R. G., Wicking, C. A.
Endosome-to-cytosol transport of viral nucleocapsids (2005)
Le Blanc, I., Luyet, P. P., Pons, V., Ferguson, C., Emans, N., Petiot, A., ...
During viral infection, fusion of the viral envelope with endosomal membranes and nucleocapsid release were thought to be concomitant events. We show here that for the vesicular stomatitis virus they...
Characterization of Rab23, a negative regulator of sonic hedgehog signaling (2005)
Evans, T. M., Simpson, F., Parton, R. G., Wicking, C.
The hedgehog signaling pathway is indispensable in embryogenesis, being responsible for the development of a wide array of vertebrate organs. Given its importance in embryogenesis, the precise...
A number of recent studies have provided new insights into the complexity of the endocytic pathways originating at the plasma membrane of mammalian cells. Many of the molecules involved in clathrin...
Lipid rafts and plasma membrane microorganization: insights from Ras (2004)
The spatial organization of plasma membrane components in discrete microdomains is thought to be a key factor in the generation of distinct signal outputs. A detailed characterization of plasma...
Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane (2004)
Rotblat, B., Prior, I. A., Muncke, C., Parton, R. G., Kloog, Y., Henis, Y. I., ...
The microlocalization of Ras proteins to different microdomains of the plasma membrane is critical for signaling specificity. Here we examine the complex membrane interactions of H-ras with a...
Expression of caveolin-1 enhances cholesterol efflux in hepatic cells (2004)
Fu, Y., Hoang, A., Escher, G., Parton, R. G., Krozowski, Z., Sviridov, D.
HepG2 cells were stably transfected with human caveolin-1 (HepG2/cav cells). Transfection resulted in expression of caveolin-1 mRNA, a high abundance of caveolin-1 protein, and the formation of...
Role of LBPA and Alix in multivesicular liposome formation and endosome organization (2004)
Matsuo, H., Chevallier, J., Mayran, N., Le Blanc, I., Ferguson, C., Faure, J., ...
What are the components that control the assembly of subcellular organelles in eukaryotic cells? Although membranes can clearly be distorted by cytosolic factors, very little is known about the...
APPL proteins link Rab5 to nuclear signal transduction via an endosomal compartment (2004)
Miaczynska, M., Christoforidis, S., Giner, A., Shevchenko, A., Uttenweiler-Joseph, S., Habermann, B., ...
Signals generated in response to extracellular stimuli at the plasma membrane are transmitted through cytoplasmic transduction cascades to the nucleus. We report the identification of a pathway...
Mounier, C. M., Ghomashchi, F., Lindsay, M. R., James, S., Singer, A. G., Parton, R. G., ...
Stable expression of human groups IIA and X secreted phospholipases A(2) (hGIIA and hGX) in CHO-K1 and HEK293 cells leads to serum- and interleukin-1beta-promoted arachidonate release. Using mutant...
Selective stimulation of caveolar endocytosis by glycosphingolipids and cholesterol (2004)
Sharma, D. K., Brown, J. C., Choudhury, A., Peterson, T. E., Holicky, E., Marks, D. L., ...
Internalization of some plasma membrane constituents, bacterial toxins, and viruses occurs via caveolae; however, the factors that regulate caveolar internalization are still unclear. Here, we...
Towler, M. C., Gleeson, P. A., Hoshino, S., Rahkila, P., Manalo, V., Ohkoshi, N., ...
The muscle isoform. of clathrin heavy chain, CHC22, has 85% sequence identity to the ubiquitously expressed CHC17, yet its expression pattern and function appear to be distinct from those of...
Association of stomatin with lipid bodies (2004)
Umlauf, E., Csaszar, E., Moertelmaier, M., Schuetz, G. J., Parton, R. G., Prohaska, R.
The oligomeric lipid raft-associated integral protein stomatin normally localizes to the plasma membrane and the late endosomal compartment. Similar to the caveolins, it is targeted to lipid bodies...
Lau, P., Nixon, S. J., Parton, R. G., Muscat, G. E. O.
The staggerer mice carry a deletion in the RORalpha gene and have a prolonged humoral response, overproduce inflammatory cytokines, and are immunodeficient. Furthermore, the staggerer mice display...
Involvement of caveolin-2 in caveolar biogenesis in MDCK cells (2003)
Lahtinen, U., Honsho, M., Parton, R. G., Simons, K., Verkade, P.
Caveolins have been identified as key components of caveolae, specialized cholesterol-enriched raft domains visible as small flask-shaped invaginations of the plasma membrane. In polarized MDCK cells...
Wyse, B. D., Prior, I. A., Qian, H. W., Morrow, I. C., Nixon, S., Muncke, C., ...
The mechanisms involved in angiotensin II type 1 receptor (AT(1)-R) trafficking and membrane localization are largely unknown. In this study, we examined the role of caveolin in these processes....
Direct visualization of Ras proteins in spatially distinct cell surface microdomains (2003)
Prior, I. A., Muncke, C., Parton, R. G., Hancock, J. F.
Localization of signaling complexes to specific micro-domains coordinates signal transduction at the plasma membrane. Using immunogold electron microscopy of plasma membrane sheets coupled with...
Emery, G., Parton, R. G., Rojo, M., Gruenberg, J.
Trans-membrane proteins of the p24 family are abundant, oligomeric proteins predominantly found in cis-Golgi membranes. They are not easily studied in vivo and their functions are controversial. We...
Lipid rafts and caveolae as portals for endocytosis: New insights and common mechanisms (2003)
Parton, R. G., Richards, A. A.
Clathrin-coated pits and caveolae are two of the most recognizable features of the plasma membrane of mammalian cells. While our understanding of the machinery regulating and driving clathrin-coated...
Paterson, A. D., Parton, R. G., Ferguson, C., Stow, J. L., Yap, A. S.
The endocytosis of E-cadherin has recently emerged as an important determinant of cadherin function with the potential to participate in remodeling adhesive contacts. In this study we focused on the...
Evans, T. M., Ferguson, C., Wainwright, B. J., Parton, R. G., Wicking, C.
The regulation of hedgehog signaling by vesicular trafficking was exemplified by the finding that Rab23, a Rab-GTPase vesicular transport protein, is mutated in open brain mice. In this study, the...
Involvement of caveolin-2 in caveolar biogenesis in MDCK cells (2003)
Lahtinen, U., Honsho, M., Parton, R. G., Simons, K., Verkade, P.
Caveolins have been identified as key components of caveolae, specialized cholesterol-enriched raft domains visible as small flask-shaped invaginations of the plasma membrane. In polarized MDCK cells...
Mayran, N., Parton, R. G., Gruenberg, J.
Proteins of the annexin family are believed to be involved in membrane-related processes, but their precise functions remain unclear. Here, we have made use of several experimental approaches,...
Flotillin-1/Reggie-2 traffics to surface raft domains via a novel golgi-independent (2002)
Roy, S., Rea, S., Martin, S., Prior, I., Prohaska, R., Hancock, J. F., ...
Morrow, I. C., Rea, S., Martin, S., Prior, I. A., Prohaska, R., Hancock, J. F., ...
Flotillins are lipid raft-associated proteins, which have been implicated in neuronal regeneration and insulin signaling. We now show that newly synthesized flotillin-1 reaches the plasma membrane...
Inhibition of lipid raft-dependent signaling by a dystrophy-associated mutant of caveolin-3 (2002)
Carozzi, A. J., Roy, S., Morrow, I. C., Pol, A., Wyse, B., Clyde-Smith, J., ...
Specific point mutations in caveolin-3, a predominantly muscle-specific member of the caveolin family, have been implicated in limb-girdle muscular dystrophy and in rippling muscle disease. We...
Differential sorting and fate of endocytosed GPI-anchored proteins (2002)
Fivaz, M., Vilbois, F., Thurnheer, S., Pasquali, C., Abrami, L., Bickel, P. E., ...
In this paper, we studied the fate of endocytosed glycosylphosphatidyl inositol anchored proteins (GPI-APs) in mammalian cells, using aerolysin, a bacterial toxin that binds to the GPI anchor, as a...
Harvey, K. F., Shearwin-Whyatt, L. M., Fotia, A., Parton, R. G., Kumar, S.
Nedd4 belongs to a family of ubiquitin-protein ligases that is characterized by 2-4 WW domains, a carboxyl-terminal Hect ((h) under bar omologous to (E) under bar6-AP (C) under bar arboxyl (t) under...
Characterization of a distinct plasma membrane macrodomain in differentiated adipocytes (2002)
Parton, R. G., Molero, Juan Carlos, Floetenmeyer, Matthias, Green, Kathryn M., James, David E.
Caveolae are small invaginations of the cell surface that are abundant in mature adipocytes. A recent study (Kanzaki, M., and Pessin, J. E. (2002) J. Biol Chem 277, 25867-25869) described novel...
Characterisation of a distinct plasma membrane macrodomain in differentiated adipocytes (2002)
Parton, R. G., Molero, JC, Floetenmyer, M., Green, K. M., James, D. E.
Caveolin and Ras function (2001)
Experimental protocols that allow confident assignment of signaling proteins to specific subdomains of the plasma membrane are essential for a full understanding of the complexities of signal...
GTP-dependent segregation of H-ras from lipid rafts is required for biological activity (2001)
Prior, I. A., Harding, A., Yan, J., Sluimer, J., Parton, R. G., Hancock, J. F.
Different sites of plasma membrane attachment may underlie functional differences between isoforms of Ras. Here we show that palmitoylation and farnesylation targets H-ras to lipid rafts and...
Rahkila, P., Takala, T. E. S., Parton, R. G., Metsikko, K.
The plasma membrane of differentiated skeletal muscle fibers comprises the sarcolemma, the transverse (T) tubule network, and the neuromuscular and muscle-tendon junctions. We analyzed the...
Flotillin-1-enriched lipid raft domains accumulate on maturing phagosomes (2001)
Dermine, J. F., Duclos, S., Garin, J., St-Louis, F., Rea, S., Parton, R. G., ...
Flotillin-1 was recently shown to be enriched on detergent-resistant domains of the plasma membrane called lipid rafts. These rafts, enriched in sphingolipids and cholesterol, sequester certain...
Cross-talk between caveolae and glycosylphosphatidylinositol-rich domains (2001)
Fivaz, M., Abrami, L., Kobayashi, T., Kinoshita, T., Parton, R. G., Van Der Groot, G. F.
Most mammalian cells have in their plasma membrane at least two types of lipid microdomains, non-invaginated lipid rafts and caveolae. Glycosylphosphatidylinositol (GPI)-anchored proteins constitute...