Leptin secretion and hypothalamic neuropeptide and receptor gene expression in sheep (2002)
Sorenson, A., Adam, C.L., Findlay, P.A., Travers, M.*, Marie, M., Thomas, L., ...
Peripheral and hypothalamic mechanisms underlying the hyperphagia of lactation have been investigated in sheep. Sheep were fed ad libitum and killed at 6 and 18 days of lactation; ad libitum-fed...
Leptin secretion and hypothalamic neuropeptide and receptor gene expression in sheep (2002)
Sorenson, A., Adam, C.L., Findlay, P.A., Travers, M., Marie, M., Thomas, L., ...
Peripheral and hypothalamic mechanisms underlying the hyperphagia of lactation have been investigated in sheep. Sheep were fed ad libitum and killed at 6 and 18 days of lactation; ad libitum-fed...
Leptin secretion and hypothalamic neuropeptide and receptor gene expression in sheep (2002)
Sorenson, A., Adam, C.L., Findlay, P.A., Travers, M., Marie, M., Thomas, L., ...
Peripheral and hypothalamic mechanisms underlying the hyperphagia of lactation have been investigated in sheep. Sheep were fed ad libitum and killed at 6 and 18 days of lactation; ad libitum-fed...
Insulin resistance of hind-limb tissues in vivo in lactating sheep.
Vernon, R G, Faulkner, A, Hay, W W, Calvert, D T, Flint, D J
1. The effects of varying the plasma insulin concentration by infusion while maintaining euglycaemia by infusion of glucose on nutrient arterio-venous differences across the hind-limb and mammary...
Vernon, R G, Piperova, L, Watt, P W, Finley, E, Lindsay-Watt, S
The mechanisms responsible for the diminished lipolytic response of adipocytes to catecholamines after litter removal from lactating rats and their modulation by growth hormone have been...
Barber, M C, Travers, M T, Finley, E, Flint, D J, Vernon, R G
The factors and mechanisms responsible for the reciprocal changes in lipogenesis in rat mammary gland and adipose tissue during the lactation cycle have been investigated. Lactation decreased the...
1. Lactation results in decreased glucose and acetate utilization and increased lactate output by sheep adipose tissue. 2. The ability of insulin to stimulate acetate uptake was lost in adipose...
1. Lactation results in a substantial fall in the rate of fatty acid synthesis in sheep adipose tissue. 2. Maintenance of adipose tissue from non-lactating sheep in tissue culture for 24 or 48 h with...
Vernon, R G, Doris, R, Finley, E, Houslay, M D, Kilgour, E, Lindsay-Watt, S
The effect of lactation on the regulation of lipolysis by beta- and alpha 2-adrenergic agents and by adenosine has been investigated. When changes in adipocyte mean cell volume (which decreases with...
Doris, R, Vernon, R G, Houslay, M D, Kilgour, E
The effect of growth hormone (GH) in vivo on the Gi-mediated anti-lipolytic signalling system of rat adipocytes has been investigated. Lowering of serum GH levels, by treatment of rats with an...
Lipolysis in rat adipocytes during recovery from lactation. Response to noradrenaline and adenosine.
The replenishment of lipid reserves of adipocytes following the removal of litters from lactating rats is associated with a 3-fold decrease in both the response of lipolysis to noradrenaline and the...
Catecholamine activation of pyruvate dehydrogenase in white adipose tissue of the rat in vivo.
Intraperitoneal injections of noradrenaline or adrenaline into rats increased the proportion of pyruvate dehydrogenase in the active state in white adipose tissue; this effect of catecholamines was...
Vernon, R G, Finley, E, Flint, D J
Removal of the litter from lactating rats results in a fall in the rate of noradrenaline-stimulated lipolysis of adipocytes. This adaptation can be prevented by administration of growth hormone...
Changes are described in the total pyruvate dehydrogenase (PDH) activity, the proportion of PDH in the active state and its control by insulin and noradrenaline in vivo, in white adipose tissue,...
Plested, C P, Taylor, E, Brindley, D N, Vernon, R G
1. The Vmax. activity of pyruvate kinase of sheep adipose tissue increased during tissue culture up to 48 h; the increase was blocked by actinomycin D (an inhibitor of transcription) and was promoted...
Protein metabolism in the mouse during pregnancy and lactation.
Millican, P E, Vernon, R G, Pain, V M
Protein synthesis was measured in vivo in the whole body and in a number of individual tissues in mice at various stages of pregnancy and lactation. The absolute rate of protein synthesis in the...
1. The mechanism responsible for the failure of insulin to activate pyruvate dehydrogenase (PDH) in white adipose tissue in vivo during lactation was investigated. 2. Insulin failed to increase PDH...
Watt, P W, Finley, E, Cork, S, Clegg, R A, Vernon, R G
1. Sheep adipose tissue retained responsiveness to catecholamines when maintained in tissue culture for 48 h; both the rate of basal lipolysis and sensitivity to beta-agonists were increased after...
Vernon, R G, Barber, M C, Finley, E
The mechanisms whereby growth hormone and dexamethasone modulate the stimulation of fatty acid synthesis by insulin in adipose tissue from lactating and non-lactating sheep have been investigated....
Adenosine and the control of lipolysis in rat adipocytes during pregnancy and lactation.
Vernon, R G, Finley, E, Taylor, E
The rate of noradrenaline-stimulated lipolysis is lower in fat-cells from lactating than from pregnant rats; this difference is eliminated by the addition of adenosine deaminase [Aitchison, Clegg &...
The effects of pregnancy and lactation on lipolysis in sheep adipose tissue in vitro were investigated. Neither pregnancy nor lactation altered the basal rate of lipolysis. The rate of...
Glucose metabolism and its regulation in pieces of perirenal adipose tissue from foetal lambs.
Robertson, J P, Faulkner, A, Vernon, R G
1. The following were measured in pieces of perirenal adipose tissue obtained from foetal lambs at about 120 days of gestation or within 3 days of term, and 9-month-old sheep: the rates of synthesis...
Lipolysis in rat adipocytes during pregnancy and lactation. The response to noradrenaline.
Aitchison, R E, Clegg, R A, Vernon, R G
1. Lipolysis has been measured in parametrial adipocytes from virgin, pregnant and lactating rats. 2. The basal rate and the maximal rate of lipolysis, the latter measured in the presence of...
Flint, D J, Sinnett-Smith, P A, Clegg, R A, Vernon, R G
Changes in the volume, the rates of fatty acid synthesis and synthesis of the glycerol moiety of acylglycerols, the activity of lipoprotein lipase, and the number and affinity of insulin receptors of...
Lipogenic enzymes in rat maternal adipose tissue in the perinatal period.
Sinnett-Smith, P A, Vernon, R G, Mayer, R J
1. The specific activities of fatty acid synthetase, acetyl-CoA carboxylase and pyruvate dehydrogenase were measured in rat adipose-tissue extracts in pregnancy and lactation. Fatty acid synthetase...
Aspects of adipose-tissue metabolism in foetal lambs.
Vernon, R G, Robertson, J P, Clegg, R A, Flint, D J
1. The mean volume of adipocytes, the rates of fatty acid and acylglycerol glycerol synthesis from various precursors (in vitro), the rates of oxidation of acetate and glucose (in vitro) and the...
Metabolism of sheep adipose tissue during pregnancy and lactation. Adaptation and regulation.
Vernon, R G, Clegg, R A, Flint, D J
1. Changes in the mean volume, the rate of fatty acid and acylglycerol glycerol synthesis, the activity of lipoprotein lipase and the numbers and affinities of insulin receptors of subcutaneous...
Triacylglycerol biosynthesis in rat adipose-tissue homogenates.
Christie, W W, Hunter, M L, Vernon, R G
The optimum cofactor requirements for triacylglycerol biosynthesis in rat adipose-tissue homogenates containing mitochondrial, microsomal and cytosolic fractions were investigated. In general the...
Glycerol metabolism in the neonatal rat
1. The possible role of glycerol as a precursor in neonatal gluconeogenesis in the rat was investigated by recording the activities of glycerol kinase and l-glycerol 3-phosphate dehydrogenase in the...
Carbohydrate formation from various precursors in neonatal rat liver
Vernon, R. G., Eaton, Susan W., Walker, D. G.
1. Measurements of the net synthesis of glucose plus glycogen from various precursors in slices of glycogen-depleted livers from rats at various stages of development indicated an increase in the...
1. The activities of some enzymes involved in both the utilization of glucose (pyruvate kinase, ATP citrate lyase, NADP-specific malate dehydrogenase, glucose 6-phosphate dehydrogenase,...
1. The effects of premature weaning on to normal and high-glucose diets and of normal weaning on to high-protein and high-fat diets on the activities of hepatic enzymes involved in both the...
Protein kinase C isoforms play differential roles in the regulation of adipocyte differentiation.
Fleming, I, MacKenzie, S J, Vernon, R G, Anderson, N G, Houslay, M D, Kilgour, E
In this study we first established, by immunoblotting with specific antibodies, the temporal changes in cellular levels of protein kinase C (PKC) isoforms during differentiation of 3T3-F442A...
Insulin resistance of hind-limb tissues in vivo in lactating sheep.
Vernon, R G, Faulkner, A, Hay, W W, Calvert, D T, Flint, D J
1. The effects of varying the plasma insulin concentration by infusion while maintaining euglycaemia by infusion of glucose on nutrient arterio-venous differences across the hind-limb and mammary...
Vernon, R G, Piperova, L, Watt, P W, Finley, E, Lindsay-Watt, S
The mechanisms responsible for the diminished lipolytic response of adipocytes to catecholamines after litter removal from lactating rats and their modulation by growth hormone have been...
Barber, M C, Travers, M T, Finley, E, Flint, D J, Vernon, R G
The factors and mechanisms responsible for the reciprocal changes in lipogenesis in rat mammary gland and adipose tissue during the lactation cycle have been investigated. Lactation decreased the...
1. Lactation results in decreased glucose and acetate utilization and increased lactate output by sheep adipose tissue. 2. The ability of insulin to stimulate acetate uptake was lost in adipose...
1. Lactation results in a substantial fall in the rate of fatty acid synthesis in sheep adipose tissue. 2. Maintenance of adipose tissue from non-lactating sheep in tissue culture for 24 or 48 h with...
Vernon, R G, Doris, R, Finley, E, Houslay, M D, Kilgour, E, Lindsay-Watt, S
The effect of lactation on the regulation of lipolysis by beta- and alpha 2-adrenergic agents and by adenosine has been investigated. When changes in adipocyte mean cell volume (which decreases with...
Doris, R, Vernon, R G, Houslay, M D, Kilgour, E
The effect of growth hormone (GH) in vivo on the Gi-mediated anti-lipolytic signalling system of rat adipocytes has been investigated. Lowering of serum GH levels, by treatment of rats with an...
Lipolysis in rat adipocytes during recovery from lactation. Response to noradrenaline and adenosine.
The replenishment of lipid reserves of adipocytes following the removal of litters from lactating rats is associated with a 3-fold decrease in both the response of lipolysis to noradrenaline and the...
Catecholamine activation of pyruvate dehydrogenase in white adipose tissue of the rat in vivo.
Intraperitoneal injections of noradrenaline or adrenaline into rats increased the proportion of pyruvate dehydrogenase in the active state in white adipose tissue; this effect of catecholamines was...
Vernon, R G, Finley, E, Flint, D J
Removal of the litter from lactating rats results in a fall in the rate of noradrenaline-stimulated lipolysis of adipocytes. This adaptation can be prevented by administration of growth hormone...
Changes are described in the total pyruvate dehydrogenase (PDH) activity, the proportion of PDH in the active state and its control by insulin and noradrenaline in vivo, in white adipose tissue,...
Plested, C P, Taylor, E, Brindley, D N, Vernon, R G
1. The Vmax. activity of pyruvate kinase of sheep adipose tissue increased during tissue culture up to 48 h; the increase was blocked by actinomycin D (an inhibitor of transcription) and was promoted...
Protein metabolism in the mouse during pregnancy and lactation.
Millican, P E, Vernon, R G, Pain, V M
Protein synthesis was measured in vivo in the whole body and in a number of individual tissues in mice at various stages of pregnancy and lactation. The absolute rate of protein synthesis in the...
1. The mechanism responsible for the failure of insulin to activate pyruvate dehydrogenase (PDH) in white adipose tissue in vivo during lactation was investigated. 2. Insulin failed to increase PDH...
Watt, P W, Finley, E, Cork, S, Clegg, R A, Vernon, R G
1. Sheep adipose tissue retained responsiveness to catecholamines when maintained in tissue culture for 48 h; both the rate of basal lipolysis and sensitivity to beta-agonists were increased after...
Vernon, R G, Barber, M C, Finley, E
The mechanisms whereby growth hormone and dexamethasone modulate the stimulation of fatty acid synthesis by insulin in adipose tissue from lactating and non-lactating sheep have been investigated....
Adenosine and the control of lipolysis in rat adipocytes during pregnancy and lactation.
Vernon, R G, Finley, E, Taylor, E
The rate of noradrenaline-stimulated lipolysis is lower in fat-cells from lactating than from pregnant rats; this difference is eliminated by the addition of adenosine deaminase [Aitchison, Clegg &...
The effects of pregnancy and lactation on lipolysis in sheep adipose tissue in vitro were investigated. Neither pregnancy nor lactation altered the basal rate of lipolysis. The rate of...
Glucose metabolism and its regulation in pieces of perirenal adipose tissue from foetal lambs.
Robertson, J P, Faulkner, A, Vernon, R G
1. The following were measured in pieces of perirenal adipose tissue obtained from foetal lambs at about 120 days of gestation or within 3 days of term, and 9-month-old sheep: the rates of synthesis...
Lipolysis in rat adipocytes during pregnancy and lactation. The response to noradrenaline.
Aitchison, R E, Clegg, R A, Vernon, R G
1. Lipolysis has been measured in parametrial adipocytes from virgin, pregnant and lactating rats. 2. The basal rate and the maximal rate of lipolysis, the latter measured in the presence of...
Flint, D J, Sinnett-Smith, P A, Clegg, R A, Vernon, R G
Changes in the volume, the rates of fatty acid synthesis and synthesis of the glycerol moiety of acylglycerols, the activity of lipoprotein lipase, and the number and affinity of insulin receptors of...
Lipogenic enzymes in rat maternal adipose tissue in the perinatal period.
Sinnett-Smith, P A, Vernon, R G, Mayer, R J
1. The specific activities of fatty acid synthetase, acetyl-CoA carboxylase and pyruvate dehydrogenase were measured in rat adipose-tissue extracts in pregnancy and lactation. Fatty acid synthetase...
Aspects of adipose-tissue metabolism in foetal lambs.
Vernon, R G, Robertson, J P, Clegg, R A, Flint, D J
1. The mean volume of adipocytes, the rates of fatty acid and acylglycerol glycerol synthesis from various precursors (in vitro), the rates of oxidation of acetate and glucose (in vitro) and the...
Metabolism of sheep adipose tissue during pregnancy and lactation. Adaptation and regulation.
Vernon, R G, Clegg, R A, Flint, D J
1. Changes in the mean volume, the rate of fatty acid and acylglycerol glycerol synthesis, the activity of lipoprotein lipase and the numbers and affinities of insulin receptors of subcutaneous...
Triacylglycerol biosynthesis in rat adipose-tissue homogenates.
Christie, W W, Hunter, M L, Vernon, R G
The optimum cofactor requirements for triacylglycerol biosynthesis in rat adipose-tissue homogenates containing mitochondrial, microsomal and cytosolic fractions were investigated. In general the...
Glycerol metabolism in the neonatal rat
1. The possible role of glycerol as a precursor in neonatal gluconeogenesis in the rat was investigated by recording the activities of glycerol kinase and l-glycerol 3-phosphate dehydrogenase in the...
Carbohydrate formation from various precursors in neonatal rat liver
Vernon, R. G., Eaton, Susan W., Walker, D. G.
1. Measurements of the net synthesis of glucose plus glycogen from various precursors in slices of glycogen-depleted livers from rats at various stages of development indicated an increase in the...
1. The activities of some enzymes involved in both the utilization of glucose (pyruvate kinase, ATP citrate lyase, NADP-specific malate dehydrogenase, glucose 6-phosphate dehydrogenase,...
1. The effects of premature weaning on to normal and high-glucose diets and of normal weaning on to high-protein and high-fat diets on the activities of hepatic enzymes involved in both the...
Protein kinase C isoforms play differential roles in the regulation of adipocyte differentiation.
Fleming, I, MacKenzie, S J, Vernon, R G, Anderson, N G, Houslay, M D, Kilgour, E
In this study we first established, by immunoblotting with specific antibodies, the temporal changes in cellular levels of protein kinase C (PKC) isoforms during differentiation of 3T3-F442A...