Chromosomal deletion and rearrangement in Streptomyces glaucescens.
Birch, A, Häusler, A, Rüttener, C, Hütter, R
The Streptomyces glaucescens genome frequently undergoes gross genomic rearrangement events which result in the deletion of extremely large segments of chromosomal DNA. The structure and origin of...
A new procedure for transformation of Amycolatopsis (Nocardia) mediterranei LBG A3136 was developed. The method makes use of polyethylene glycol and alkaline cations and enables direct transformation...
Schmidheini, T, Sperisen, P, Paravicini, G, Hütter, R, Braus, G
The Saccharomyces cerevisiae ARO7 gene product chorismate mutase, a single-branch-point enzyme in the aromatic amino acid biosynthetic pathway, is activated by tryptophan and subject to feedback...
Tryptophan biosynthesis in Saccharomyces cerevisiae: control of the flux through the pathway.
Miozzari, G, Niederberger, P, Hütter, R
Enzyme derepression and feedback inhibition of the first enzyme are the regulatory mechanisms demonstrated for the tryptophan pathway in Saccharomyces cerevisiae. The relative contributions of the...
Grossenbacher, H, Horn, C, Cook, A M, Hütter, R
Pseudomonas sp. strain A grew with 2-chloro-1,3,5-triazine-4,6-diamine as the sole and growth-limiting source of nitrogen. The substrate was utilized quantitatively and concomitantly with growth and...
Schürch, A., Miozzari, J., Hütter, R.
In a wild-type strain of Saccharomyces cerevisiae the tryptophan analogue dl-5-methyl-tryptophan (5MT) causes only a slight reduction of the growth rate. Uptake experiments indicate that the limited...
Organization of the Tryptophan Pathway: a Phylogenetic Study of the Fungi
The enzymes involved in tryptophan biosynthesis have been analyzed in a variety of fungal strains and a few other microorganisms. The same five biosynthetic reactions occur in all organisms tested,...
The TRP4 gene of Saccharomyces cerevisiae: isolation and structural analysis.
Furter, R, Paravicini, G, Aebi, M, Braus, G, Prantl, F, Niederberger, P, ...
The TRP4 gene of Saccharomyces cerevisiae, encoding the anthranilate phosphoribosyl transferase, was isolated and subcloned by functional complementation in yeast. A 2 kb fragment containing...
Biological role of the general control of amino acid biosynthesis in Saccharomyces cerevisiae.
Niederberger, P, Miozzari, G, Hütter, R
The biological role of the "general control of amino acid biosynthesis" has been investigated by analyzing growth and enzyme levels in wild-type, bradytrophic, and nonderepressing mutant strains of...
Interpathway regulation of the TRP4 gene of yeast.
Braus, G, Mösch, H U, Vogel, K, Hinnen, A, Hütter, R
Two regulatory proteins, PHO2 and the general control regulator GCN4, bind in vitro to the promoter of the tryptophan biosynthetic TRP4 gene; the TRP4 gene product catalyses the phosphoribosylation...
Bacterial degradation of N-cyclopropylmelamine. The steps to ring cleavage.
Cook, A M, Grossenbacher, H, Hütter, R
The s-triazine cyclopropylmelamine (N-cyclopropyl-1,3,5-triazine-2,4,6-triamine) was degraded to about 6 mol of NH4+/mol of substrate by a mixture of two bacteria (strains A and D, both Pseudomonas...
Ring cleavage and degradative pathway of cyanuric acid in bacteria.
Cook, A M, Beilstein, P, Grossenbacher, H, Hütter, R
The degradative pathway of cyanuric acid [1,3,5-triazine-2,4,6(1H,3H,5H)-trione] was examined in Pseudomonas sp. strain D. The bacterium grew with cyanuric acid, biuret, urea or NH4+ as sole source...
The degradative pathway of the s-triazine melamine. The steps to ring cleavage.
Jutzi, K, Cook, A M, Hütter, R
1. The degradative pathway of melamine (1,3,5-triazine-2,4,6-triamine) was examined in Pseudomonas sp. strain A. 2. The bacterium grew with melamine, ammeline, ammelide, cyanuric acid or NH+4 as sole...
Chromosomal deletion and rearrangement in Streptomyces glaucescens.
Birch, A, Häusler, A, Rüttener, C, Hütter, R
The Streptomyces glaucescens genome frequently undergoes gross genomic rearrangement events which result in the deletion of extremely large segments of chromosomal DNA. The structure and origin of...
A new procedure for transformation of Amycolatopsis (Nocardia) mediterranei LBG A3136 was developed. The method makes use of polyethylene glycol and alkaline cations and enables direct transformation...
Schmidheini, T, Sperisen, P, Paravicini, G, Hütter, R, Braus, G
The Saccharomyces cerevisiae ARO7 gene product chorismate mutase, a single-branch-point enzyme in the aromatic amino acid biosynthetic pathway, is activated by tryptophan and subject to feedback...
Tryptophan biosynthesis in Saccharomyces cerevisiae: control of the flux through the pathway.
Miozzari, G, Niederberger, P, Hütter, R
Enzyme derepression and feedback inhibition of the first enzyme are the regulatory mechanisms demonstrated for the tryptophan pathway in Saccharomyces cerevisiae. The relative contributions of the...
Grossenbacher, H, Horn, C, Cook, A M, Hütter, R
Pseudomonas sp. strain A grew with 2-chloro-1,3,5-triazine-4,6-diamine as the sole and growth-limiting source of nitrogen. The substrate was utilized quantitatively and concomitantly with growth and...
Schürch, A., Miozzari, J., Hütter, R.
In a wild-type strain of Saccharomyces cerevisiae the tryptophan analogue dl-5-methyl-tryptophan (5MT) causes only a slight reduction of the growth rate. Uptake experiments indicate that the limited...
Organization of the Tryptophan Pathway: a Phylogenetic Study of the Fungi
The enzymes involved in tryptophan biosynthesis have been analyzed in a variety of fungal strains and a few other microorganisms. The same five biosynthetic reactions occur in all organisms tested,...
The TRP4 gene of Saccharomyces cerevisiae: isolation and structural analysis.
Furter, R, Paravicini, G, Aebi, M, Braus, G, Prantl, F, Niederberger, P, ...
The TRP4 gene of Saccharomyces cerevisiae, encoding the anthranilate phosphoribosyl transferase, was isolated and subcloned by functional complementation in yeast. A 2 kb fragment containing...
Biological role of the general control of amino acid biosynthesis in Saccharomyces cerevisiae.
Niederberger, P, Miozzari, G, Hütter, R
The biological role of the "general control of amino acid biosynthesis" has been investigated by analyzing growth and enzyme levels in wild-type, bradytrophic, and nonderepressing mutant strains of...
Interpathway regulation of the TRP4 gene of yeast.
Braus, G, Mösch, H U, Vogel, K, Hinnen, A, Hütter, R
Two regulatory proteins, PHO2 and the general control regulator GCN4, bind in vitro to the promoter of the tryptophan biosynthetic TRP4 gene; the TRP4 gene product catalyses the phosphoribosylation...
Bacterial degradation of N-cyclopropylmelamine. The steps to ring cleavage.
Cook, A M, Grossenbacher, H, Hütter, R
The s-triazine cyclopropylmelamine (N-cyclopropyl-1,3,5-triazine-2,4,6-triamine) was degraded to about 6 mol of NH4+/mol of substrate by a mixture of two bacteria (strains A and D, both Pseudomonas...
Ring cleavage and degradative pathway of cyanuric acid in bacteria.
Cook, A M, Beilstein, P, Grossenbacher, H, Hütter, R
The degradative pathway of cyanuric acid [1,3,5-triazine-2,4,6(1H,3H,5H)-trione] was examined in Pseudomonas sp. strain D. The bacterium grew with cyanuric acid, biuret, urea or NH4+ as sole source...
The degradative pathway of the s-triazine melamine. The steps to ring cleavage.
Jutzi, K, Cook, A M, Hütter, R
1. The degradative pathway of melamine (1,3,5-triazine-2,4,6-triamine) was examined in Pseudomonas sp. strain A. 2. The bacterium grew with melamine, ammeline, ammelide, cyanuric acid or NH+4 as sole...