Functional reconstruction of structurally complex epitopes using CLIPS™ technology (2009)
Timmerman, P., Puijk, W.C., Boshuizen, R.S., Dijken, P. Van, Slootstra, J.W., Beurskens, F.J., ...
This review summarizes an illustrative set of data from our research on the reconstruction of structurally complex protein surfaces, i.e. those that fail to be mimicked properly by linear peptides....
Timmerman, P., Barderas, R., Desmet, J., Altschuh, D., Shochat, S., Hollestelle, M.J., ...
The great success of therapeutic monoclonal antibodies has fueled research toward mimicry of their binding sites and the development of new strategies for peptide-based mimetics production. Here, we...
Hollestelle, M.J., Timmerman, P., Meloen, R.H., Höppener, J.W.M.
The interaction of gastrin with the cholecystokinin 2 (CCK2)/gastrin receptor has been studied extensively in relation to gastric acid secretion. However, not much is known about the contribution of...
Monitoring health by values of acute phase proteins (2006)
Gruys, E., Toussaint, M.J.M., Niewold, T.A., Koopmans, S.J., Meloen, R.H.
A systemic acute phase reaction may develop during infection and inflammation, due to the action of peripherally liberated proinflammatory cytokines. Hepatic metabolism changes, and negative and...
Monitoring health by values of acute phase proteins (2006)
Gruys, E., Toussaint, M.J.M., Niewold, T.A., Koopmans, S.J., Dijk, E. Van, Meloen, R.H.
Effects of GnRH immunization in sexually mature pony stallions (2005)
Turkstra, J.A., Knaap, J., Rottier, P.J.M., Teerds, K.J., Colenbrander, B., ...
Immunization against gonadotrophin releasing hormone (GnRH) was studied as an alternative for the commonly used surgical castration in stallions. Two GnRH vaccines comprising non-mineral oil...
Effects of GnRH immunization in sexually mature pony stallions (2005)
Turkstra, J.A., Knaap, J., Rottier, P.J.M., Teerds, K.J., Colenbrander, B.A., ...
Marissen, W.E., Kramer, R.A., Rice, A., Weldon, W.C., Niezgoda, M., Faber, M., ...
Anti-rabies virus immunoglobulin combined with rabies vaccine protects humans from lethal rabies infections. For cost and safety reasons, replacement of the human or equine polyclonal immunoglobulin...
Turkstra, J.A., Schaaper, W.M.M., Oonk, H.B., Meloen, R.H.
Gonadotropin releasing hormone (GnRH) occurs in various isoforms in mammals, i.e. GnRH-I (mammalian GnRH), GnRH-II (chicken GnRH-II), GnRH-III (salmon GnRH) and two forms of lamprey GnRH. The...
Boshuizen, R.S., Langeveld, J.P.M., Salmona, M., Williams, A., Meloen, R.H., Langendijk, J.P.
Transmissible spongiform encephalopathies are neurodegenerative diseases and are considered to be caused by malformed prion proteins accumulated into fibrillar structures that can then aggregate to...
Identification of an interleukin-15 alpha receptor-binding site on human interleukin-15 (2004)
Bernard, J., Harb, C., Mortier, E., Quemener, A., Meloen, R.H., Vermot-Desroches, C., ...
To identify the epitopes in human Interleukin-15 (IL-15) that are responsible for binding to the interleukin-15 receptor -chain, antibody as well as receptor mapping by peptide scanning and...
The efficacy of GnRH vaccines in controlling reproductive functions in horses (2004)
Stout, T.A.E., Turkstra, J.A., Meloen, R.H., Colenbrander, B.A.
Discovery and in vivo evaluation of new melanocortin-4 receptor selective peptides (2003)
Gispen, W.H., Nijenhuis, W.A., Kruijtzer, J.A., Wanders, N., Vrinten, D.H., Garner, K.M., ...
The melanocortin-4 receptor (MC4R) is involved in several physiological processes, including body weight regulation and grooming behaviour in rats. It has also been suggested that the MC4R mediates...
Scheffer, G.L., Broxterman, H.J., Hooijberg, J.H., Slootstra, J.W., Meloen, R.H., ...
Tumor cells may become resistant to conventional anticancer drugs through the occurrence of transmembrane transporter proteins such as P-glycoprotein (ABCB1), breast cancer resistance protein...
Discovery and in vivo evaluation of new melanocortin-4 receptor-selective peptides (2003)
Nijenhuis, W.A.J., Kruijtzer, J.A.W., Wanders, N., Vrinten, D.H., Garner, K.M., Schaaper, W.M.M., ...
The melanocortin-4 receptor (MC4R) is involved in several physiological processes, including body weight regulation and grooming behaviour in rats. It has also been suggested that the MC4R mediates...
Discovery and in vivo evaluation of new melanocortin-4 receptor-selective peptides. (2003)
Nijenhuis, W.A.J., Kruijtzer, J.A.W., Wanders, N., Vrinten, D.H., Garner, K.M., Schaaper, W.M.M., ...
Langedijk, J.P.M., Schaaper, W.M.M., Meloen, R.H., Hulst, M.M.
Erns is a pestivirus envelope glycoprotein and is the only known viral surface protein with RNase activity. Erns is a disulfide-linked homodimer of 100 kDa; it is found on the surface of...
Turkstra, J.A., Jongbloed, A.W., Oonk, H.B., Meloen, R.H.
In this study, the performance of male pigs immunized against GnRH was determined in relation to the onset of their biological response to the immunization. Pigs were immunized at 9 and 17 wk of age...
Zeng, X.Y., Turkstra, J.A., Meloen, R.H., Liu, X.Y., Chen, F.Q., Schaaper, W.M.M., ...
The objective of this study was to determine the optimal dose of a GnRH vaccine for immunocastration of Chinese male pigs, based on immune, endocrine and testicular responses. Forty-two crossbred...
Zeng, X.Y., Turkstra, J.A., Tsigos, A., Meloen, R.H., Liu, X.Y., Chen, F.Q., ...
Surgical castration of young female pigs is common practice in Chinese pig farming today. The purpose of the present study is to investigate anti-GnRH immunization as a practical alternative to...
Torfs, H., Akerman, K.E., Nachman, R.J., Oonk, H.B., Detheux, M., Poels, J., ...
The activity of a series of synthetic tachykinin-like peptide analogs was studied by means of microscopic calcium imaging on recombinant neurokinin receptor expressing cell lines. A C-terminal...
Functional mimicry of a discontinuous antigenic site by a designed synthetic peptide (2002)
Villen, J., Borras, E., Schaaper, W.M.M., Meloen, R.H., Davila, M., Domingo, E., ...
Functional reproduction of the discontinuous antigenic site D of foot-and-mouth disease virus (FMDV) has been achieved by means of synthetic peptide constructions that integrate each of the three...
Schaaper, W.M.M., Langeveld, J.P.M., Boshuizen, R.S., Meloen, R.H.
The influence of the nature of the bond between a peptide and a (lipidic) carrier molecule on the immunogenicity of that construct was investigated. As types of bonds a thioester-, a disulfide-, an...
Langedijk, J.P., Middel, W.G., Meloen, R.H., Kramps, J.A.
Peptides deduced from the C-terminal end (residues 191 to 227) of pestivirus envelope protein Erns were used to develop enzyme-linked immunosorbent assays (ELISAs) to measure specifically antibodies...
Characterisation of a protective linear B cell epitope against feline parvoviruses (2001)
Langeveld, J.P., Martinez Torrecuadrada, J., Boshuizen, R.S., Meloen, R.H., Ignacio Casal, J.
Chaperonnes, deelproject 4 Rapportage Technology Scan (2001)
Meloen, R.H., Oonk, H.B., Swanenburg, M., Rijsman, D.F.M., Leloux, M.S., ...
Assessment of health in pigs by acute phase protein assays (2000)
Toussaint, M.J.M., Lipperheide, C., Eckersall, P.D., Alava, M., Jobert, J.L., Heegaard, P.M.H., ...
Martinez-Torrecuadrada, J.L., Langeveld, J.P.M., Venteo, A., Sanz, A., Dalsgaard, K., Hamilton, W.D.O., ...
Skraban, R., Matthiasdottir, S., Torsteindottir, S., Agnarsdottir, G., Gudmundsson, B., Georgsson, G., ...
Gispen, W.H., Adan, R.A.H., Szklarczyk, A.W., Oosterom, J., Brakkee, J.H., Nijenhuis, W.A., ...
Since the melanocortin MC3 and melanocortin MC4 receptors are the main melanocortin receptor subtypes expressed in rat brain, we characterized the activity and affinity of nine melanocortin receptor...
Gispen, W.H., Oosterom, J., Nijenhuis, W.A., Schaaper, W.M., Slootstra, J., Meloen, R.H., ...
Melanocortin peptides regulate a variety of physiological processes. Five melanocortin receptors (MC-R) have been cloned and the MC3R and MC4R are the main brain MC receptors. The aim of this study...
Adan, R.A.H., Szklarczyk, A.W., Oosterom, J., Brakkee, J.H., Nijenhuis, W.A.J., Schaaper, W.M.M., ...
Antigenic structure of the capsid protein of rabbit haemorrhagic disease virus (1998)
Martinez-Torrecuadrada, J.L., Cortés, E., Vela, C., Langeveld, J.P.M., Meloen, R.H., Dalsgaard, K., ...
Immunodominance does not result from peptide competition for MHC class II presentation (1998)
Lo-Man, R., Langeveld, J.P.M., Martineau, P., Hofnung, M., Meloen, R.H., Leclerc, C.
Zijlstra-Westhoff, W.E., Slootstra, J.W., Puijk, W.C., Schaaper, W.M.M., Oonk, H.B., Meloen, R.H.
The aim of the present work was to define an FSH receptor (FSHR) peptide that can induce antibodies that will inhibit the bioactivity of FSH. Therefore, the hFSHR sequence was aligned with that of...
Synthesis and activity of small peptides from radish antifungal protein (1998)
Schaaper, W.M.M., Posthuma, G.A., Plasman, H.H., Broekaert, W.F., ...
Synthesis of cyclic alpha-MSH peptides (1998)
Schaaper, W.M.M., Adan, R.A.H., Posthuma, T.A., Oosterom, J., Gispen, W.H., Meloen, R.H.
Active immunization against gonadotrophin-releasing hormone in male pigs (1998)
Zeng, X.Y., Turkstra, J.A., Guo, D.Z., Liu, X.Y., Meloen, R.H., ...
Synthesis of cyclic α-MSH peptides (1998)
Gispen, W.H., Schaaper, W.M.M.., Adan, R.A.H., Posthuma, T.A., Oosterom, J., Meloen, R.H.
Cyclic lactam analogs of -melanocyte stimulating hormone (-MSH) have been shown to be potent agonists in the frog skin bioassay [Al-Obeidi, F. et al., J. Med. Chem., 32 (1989) 2555], demonstrating...
Synthesis of cyclic a-MSH peptides. (1998)
Schaaper, W.M.M., Adan, R.A.H., Posthuma, T.A., Oosterom, J., Gispen, W.H., Meloen, R.H.
Langedijk, J.P.M., Brandenburg, A.H., Middel, W.G.J., Meloen, R.H., Van Oirschot, J.T.
Peptides deduced from the central conserved region (residues 158 to 189) of protein G of human respiratory syncytial virus (HRSV) subtypes A and B were used as antigens in subtype-specific...
Synthesis and activity of small peptides from Radish Antifungal Protein (1996)
Schaaper, W.M.M., Posthuma, G.A., Plasman, H.H., Broekaert, W.F., ...
Langedijk, J.P.M., Middel, W.G.J., Schaaper, W.M.M., Meloen, R.H., Kramps, J.A., Brandenburg, A.H., ...
Peptides deduced from the central hydrophobic region (residues 158-189) of the G protein of bovine and ovine respiratory syncytial virus (RSV) and of human RSV subtypes A and B were synthesized....
Siebelink, K.H.J., Bosch, M.L., Rimmelzwaan, G.F., Meloen, R.H.
Viral progeny of two molecular clones of feline immunodeficiency virus (FIV), 19k1 and 19k32, were tested in a virus neutralization assay. In this assay the infection of thymocytes with FIV19k1 was...
Schutten, M., Langedijk, J.P.M., Andeweg, A.C., Huisman, R.C., Meloen, R.H.
A type-specific human immunodeficiency virus type 1 (HIV-1)-neutralizing human monoclonal antibody (HuMAb MN215) is described that reacts with the V3 domain of a number of subtype B virus strains....
Testis size after immunocastration as parameter for the absence of boar taint (1995)
Oonk, H.B., Turkstra, J.A., Lankhof, H., Schaaper, W.M.M., Verheijden, J.H.M., Meloen, R.H.
Domains of rat interleukin-1ß involved in type 1 receptor binding (1995)
Schotanus, K., Holtkamp, G.M., Meloen, R.H., Puijk, W.C., Berkenbosch, F., Tilders, F.J.H.
PEPSCAN to determine T and B cell epitopes (1995)
Meloen, R.H., Puijk, W.C., Langeveld, J.P.M., Langedijk, J.P.M., Schaaper, W.M.M.
Possible Active Site of the Sweet-tasting Protein Thaumatin (1995)
Slootstra, J.W., De Geus, P., Haas, H., Verrips, C.T., Meloen, R.H.
Epitopes on thaumatin and monellin were studied using the PEPSCAN-technology. The antibodies used were raised against thaumatin. Only antibodies that, in an ELISA, both recognized thaumatin and...
Langeveld, J.P.M., Ignacio Casal, J., Cortes, E., Swart, R.L. De, Vela, C., ...
A synthetic peptide vaccine which protects dogs against challenge with virulent canine parvovirus is described. The amino acid sequence used was discovered in previous studies on the immunogenic...
Langeveld, J.P.M., Casal, J.I., Cortes, E., Swart, R.L. De, Vela, C., ...
A synthetic peptide vaccine which protects dogs against challenge with virulent canine parvovirus is described. The amino acid sequence used was discovered in previous studies on the immunogenic...
Siebelink, K.H.J., Rimmelzwaan, G.F., Bosch, M.L., Meloen, R.H.
We infected a specific-pathogen-free cat (cat 14) with molecularly cloned feline immunodeficiency virus clone 19k1 (FIV19k1 [K. H. J. Siebelink, I. Chu, G. F. Rimmelzwaan, K. Weijer, A. D. M. E....
Weijer, K., Pfauth, A., Herwijnen, R. Van, Jarrett, O., Meloen, R.H., Tomee, C., ...
The surface glycoprotein, gp70, of feline leukaemia virus (FeLV) contains sites which are important in inducing neutralizing antibodies. Using synthetic peptides corresponding to nucleotide sequence...
Langedijk, J.P.M., Groen, J., Meloen, R.H.
The core of a unique linear neutralization epitope (G5) on the glycoprotein of rabies virus, recognized by a virus-neutralizing mouse monoclonal antibody (MAb 6-15C4), was determined by Pepscan...
Rimmelzwaan, G.F., Poelen, M.C.M., Meloen, R.H., Carlson, J.
Three synthetic peptides derived from the amino acid sequence of VP2 of canine parvovirus (CPV) which were recently shown to represent three distinct T cell epitopes for BALB/c mice could prime...
Javaherian, K, Langlois, A J, Schmidt, S, Kaufmann, M, Cates, N, Langedijk, J P, ...
To identify the principal neutralization determinant (PND) of simian immunodeficiency virus (SIV), antisera were generated using recombinant gp110 [the SIV analog of the human immunodeficiency virus...
Kast, W M, Roux, L, Curren, J, Blom, H J, Voordouw, A C, Meloen, R H, ...
The only peptide of Sendai virus that is recognized by cytotoxic T lymphocytes (CTL) in B6 mice was found with (i) the use of recombinant vaccinia virus constructs containing separate genes of Sendai...
Langedijk, J. P. M., Middel, W. G. J., Meloen, R. H., Kramps, J. A., De Smit, J. A.
Peptides deduced from the C-terminal end (residues 191 to 227) of pestivirus envelope protein Erns were used to develop enzyme-linked immunosorbent assays (ELISAs) to measure specifically antibodies...
Langedijk, J. P. M., Van Veelen, P. A., Schaaper, W. M. M., De Ru, A. H., Meloen, R. H., Hulst, M. M.
Erns is a pestivirus envelope glycoprotein and is the only known viral surface protein with RNase activity. Erns is a disulfide-linked homodimer of 100 kDa; it is found on the surface of...
Duim, B, Vogel, L, Puijk, W, Jansen, H M, Meloen, R H, Dankert, J, ...
Antigenic drift of the major outer membrane protein (MOMP) P2 of nonencapsulated Haemophilus influenzae as observed during persistent infections in patients with chronic bronchitis was mimicked in a...
Casal, J I, Langeveld, J P, Cortés, E, Schaaper, W W, Van Dijk, E, Vela, C, ...
The N-terminal domain of the major capsid protein VP2 of canine parvovirus was shown to be an excellent target for development of a synthetic peptide vaccine, but detailed information about number of...
Langedijk, J P, Meloen, R H, Taylor, G, Furze, J M, Van Oirschot, J T
Epitopes were resolved at the amino acid level for nine monoclonal antibodies (MAbs) directed against the central conserved region of protein G of bovine respiratory syncytial virus (BRSV-G). Peptide...
Immunohistochemical detection of prion protein in lymphoid tissues of sheep with natural scrapie.
Van Keulen, L J, Schreuder, B E, Meloen, R H, Mooij-Harkes, G, Vromans, M E, Langeveld, J P
The scrapie-associated form of the prion protein (PrPSc) accumulates in the brain and lymphoid tissues of sheep with scrapie. In order to assess whether detecting PrPSc in lymphoid tissue could be...
Langedijk, J P, Brandenburg, A H, Middel, W G, Osterhaus, A, Meloen, R H, Van Oirschot, J T
Peptides deduced from the central conserved region (residues 158 to 189) of protein G of human respiratory syncytial virus (HRSV) subtypes A and B were used as antigens in subtype-specific...
Langeveld, J P, Casal, J I, Osterhaus, A D, Cortés, E, De Swart, R, Vela, C, ...
A synthetic peptide vaccine which protects dogs against challenge with virulent canine parvovirus is described. The amino acid sequence used was discovered in previous studies on the immunogenic...
Langeveld, J P, Casal, J I, Vela, C, Dalsgaard, K, Smale, S H, Puijk, W C, ...
Ten antigenic sites on canine parvovirus (CPV) were mapped with a complete set of overlapping nonapeptides of the capsid proteins VP1 and VP2: five of these sites were recognized by sera from...
Siebelink, K H, Rimmelzwaan, G F, Bosch, M L, Meloen, R H, Osterhaus, A D
We infected a specific-pathogen-free cat (cat 14) with molecularly cloned feline immunodeficiency virus clone 19k1 (FIV19k1 [K. H. J. Siebelink, I. Chu, G. F. Rimmelzwaan, K. Weijer, A. D. M. E....
Brown, C S, Jensen, T, Meloen, R H, Puijk, W, Sugamura, K, Sato, H, ...
An immunodominant region on baculovirus-produced parvovirus B19 VP2 capsids was localized between amino acids 259 and 426 by mapping the binding sites of a panel of monoclonal antibodies which...
Brake, D A, Goudsmit, J, Krone, W J, Schammel, P, Appleby, N, Meloen, R H, ...
A panel of murine monoclonal antibodies (MAbs) to the human immunodeficiency virus type 1 trans-activator tat protein were characterized. The anti-tat MAbs were mapped to the different domains of the...
Analysis and simulation of a neutralizing epitope of transmissible gastroenteritis virus.
Posthumus, W P, Lenstra, J A, Schaaper, W M, Van Nieuwstadt, A P, Enjuanes, L, Meloen, R H
The amino acid sequences recognized by monoclonal antibodies (MAbs) specific for the antigenic site IV of the spike protein S of transmissible gastroenteritis virus were analyzed by PEPSCAN. All MAbs...
Noordhoek, G T, Cockayne, A, Schouls, L M, Meloen, R H, Stolz, E, Van Embden, J D
In an effort to serologically differentiate syphilis from yaws, 69 monoclonal antibody species raised against Treponema pallidum subsp. pallidum were tested by immunoblotting for their reactivity...
Goudsmit, J, Debouck, C, Meloen, R H, Smit, L, Bakker, M, Asher, D M, ...
Chimpanzees are susceptible to infection by divergent strains of human immunodeficiency virus type 1 (HIV-1), none of which cause clinical or immunological abnormalities. Chimpanzees were inoculated...
Oftung, F, Geluk, A, Lundin, K E, Meloen, R H, Thole, J E, Mustafa, A S, ...
By combining a DNA subclone and synthetic-peptide approach, we mapped epitopes of the immunogenic mycobacterial 70-kDa heat shock protein (HSP70) recognized by human CD4+ T-cell clones and lines. In...
Geysen, H M, Meloen, R H, Barteling, S J
A procedure is described for rapid concurrent synthesis on solid supports of hundreds of peptides, of sufficient purity to react in an enzyme-linked immunosorbent assay. Interaction of synthesized...
Geysen, H M, Barteling, S J, Meloen, R H
Antisera were raised against the chemically synthesized peptide corresponding to each epitope of three foot-and-mouth disease virus strains. Peptide synthesis was further used to determine which...
Puijk, W C, Plasman, H H, Kuperus, D, Schaaper, W M, Beekman, N J, ...
Follicle-stimulating hormone (follitropin, FSH) belongs to a group of closely related glycoprotein hormones that contain two noncovalently linked dissimilar subunits designated alpha and beta. By...
Javaherian, K, Langlois, A J, Schmidt, S, Kaufmann, M, Cates, N, Langedijk, J P, ...
To identify the principal neutralization determinant (PND) of simian immunodeficiency virus (SIV), antisera were generated using recombinant gp110 [the SIV analog of the human immunodeficiency virus...
Kast, W M, Roux, L, Curren, J, Blom, H J, Voordouw, A C, Meloen, R H, ...
The only peptide of Sendai virus that is recognized by cytotoxic T lymphocytes (CTL) in B6 mice was found with (i) the use of recombinant vaccinia virus constructs containing separate genes of Sendai...
Langedijk, J. P. M., Middel, W. G. J., Meloen, R. H., Kramps, J. A., De Smit, J. A.
Peptides deduced from the C-terminal end (residues 191 to 227) of pestivirus envelope protein Erns were used to develop enzyme-linked immunosorbent assays (ELISAs) to measure specifically antibodies...
Langedijk, J. P. M., Van Veelen, P. A., Schaaper, W. M. M., De Ru, A. H., Meloen, R. H., Hulst, M. M.
Erns is a pestivirus envelope glycoprotein and is the only known viral surface protein with RNase activity. Erns is a disulfide-linked homodimer of 100 kDa; it is found on the surface of...
Duim, B, Vogel, L, Puijk, W, Jansen, H M, Meloen, R H, Dankert, J, ...
Antigenic drift of the major outer membrane protein (MOMP) P2 of nonencapsulated Haemophilus influenzae as observed during persistent infections in patients with chronic bronchitis was mimicked in a...
Casal, J I, Langeveld, J P, Cortés, E, Schaaper, W W, Van Dijk, E, Vela, C, ...
The N-terminal domain of the major capsid protein VP2 of canine parvovirus was shown to be an excellent target for development of a synthetic peptide vaccine, but detailed information about number of...
Langedijk, J P, Meloen, R H, Taylor, G, Furze, J M, Van Oirschot, J T
Epitopes were resolved at the amino acid level for nine monoclonal antibodies (MAbs) directed against the central conserved region of protein G of bovine respiratory syncytial virus (BRSV-G). Peptide...
Immunohistochemical detection of prion protein in lymphoid tissues of sheep with natural scrapie.
Van Keulen, L J, Schreuder, B E, Meloen, R H, Mooij-Harkes, G, Vromans, M E, Langeveld, J P
The scrapie-associated form of the prion protein (PrPSc) accumulates in the brain and lymphoid tissues of sheep with scrapie. In order to assess whether detecting PrPSc in lymphoid tissue could be...
Langedijk, J P, Brandenburg, A H, Middel, W G, Osterhaus, A, Meloen, R H, Van Oirschot, J T
Peptides deduced from the central conserved region (residues 158 to 189) of protein G of human respiratory syncytial virus (HRSV) subtypes A and B were used as antigens in subtype-specific...
Langeveld, J P, Casal, J I, Osterhaus, A D, Cortés, E, De Swart, R, Vela, C, ...
A synthetic peptide vaccine which protects dogs against challenge with virulent canine parvovirus is described. The amino acid sequence used was discovered in previous studies on the immunogenic...
Langeveld, J P, Casal, J I, Vela, C, Dalsgaard, K, Smale, S H, Puijk, W C, ...
Ten antigenic sites on canine parvovirus (CPV) were mapped with a complete set of overlapping nonapeptides of the capsid proteins VP1 and VP2: five of these sites were recognized by sera from...
Siebelink, K H, Rimmelzwaan, G F, Bosch, M L, Meloen, R H, Osterhaus, A D
We infected a specific-pathogen-free cat (cat 14) with molecularly cloned feline immunodeficiency virus clone 19k1 (FIV19k1 [K. H. J. Siebelink, I. Chu, G. F. Rimmelzwaan, K. Weijer, A. D. M. E....
Brown, C S, Jensen, T, Meloen, R H, Puijk, W, Sugamura, K, Sato, H, ...
An immunodominant region on baculovirus-produced parvovirus B19 VP2 capsids was localized between amino acids 259 and 426 by mapping the binding sites of a panel of monoclonal antibodies which...
Brake, D A, Goudsmit, J, Krone, W J, Schammel, P, Appleby, N, Meloen, R H, ...
A panel of murine monoclonal antibodies (MAbs) to the human immunodeficiency virus type 1 trans-activator tat protein were characterized. The anti-tat MAbs were mapped to the different domains of the...
Analysis and simulation of a neutralizing epitope of transmissible gastroenteritis virus.
Posthumus, W P, Lenstra, J A, Schaaper, W M, Van Nieuwstadt, A P, Enjuanes, L, Meloen, R H
The amino acid sequences recognized by monoclonal antibodies (MAbs) specific for the antigenic site IV of the spike protein S of transmissible gastroenteritis virus were analyzed by PEPSCAN. All MAbs...
Noordhoek, G T, Cockayne, A, Schouls, L M, Meloen, R H, Stolz, E, Van Embden, J D
In an effort to serologically differentiate syphilis from yaws, 69 monoclonal antibody species raised against Treponema pallidum subsp. pallidum were tested by immunoblotting for their reactivity...
Goudsmit, J, Debouck, C, Meloen, R H, Smit, L, Bakker, M, Asher, D M, ...
Chimpanzees are susceptible to infection by divergent strains of human immunodeficiency virus type 1 (HIV-1), none of which cause clinical or immunological abnormalities. Chimpanzees were inoculated...
Oftung, F, Geluk, A, Lundin, K E, Meloen, R H, Thole, J E, Mustafa, A S, ...
By combining a DNA subclone and synthetic-peptide approach, we mapped epitopes of the immunogenic mycobacterial 70-kDa heat shock protein (HSP70) recognized by human CD4+ T-cell clones and lines. In...
Geysen, H M, Meloen, R H, Barteling, S J
A procedure is described for rapid concurrent synthesis on solid supports of hundreds of peptides, of sufficient purity to react in an enzyme-linked immunosorbent assay. Interaction of synthesized...
Geysen, H M, Barteling, S J, Meloen, R H
Antisera were raised against the chemically synthesized peptide corresponding to each epitope of three foot-and-mouth disease virus strains. Peptide synthesis was further used to determine which...
Puijk, W C, Plasman, H H, Kuperus, D, Schaaper, W M, Beekman, N J, ...
Follicle-stimulating hormone (follitropin, FSH) belongs to a group of closely related glycoprotein hormones that contain two noncovalently linked dissimilar subunits designated alpha and beta. By...
Mustafa, A S, Lundin, K E, Meloen, R H, Shinnick, T M, Coulson, A F, Oftung, F
The mycobacterial 60,000 MW heat shock protein (hsp 60) is a major antigen recognized by mycobacteria-reactive human CD4+ T cells with lymphokine profiles and effector functions consistent with...
Van Lierop, M J, Van Maanen, K, Meloen, R H, Rutten, V P, De Jong, M A, Hensen, E J
We studied proliferative responses of bovine T lymphocytes to foot-and-mouth disease virus (FMDV) serotypes A, O and C as well as to three peptides including the two major B-cell epitopes of FMDV...
Piccardo, P., Langeveld, J. P., Hill, A. F., Dlouhy, S. R., Young, K., Giaccone, G., ...
Antibodies to the prion protein (PrP) have been critical to the neuropathological and biochemical characterization of PrP-related degenerative diseases in humans and animals. Although PrP is highly...
Mustafa, A S, Lundin, K E A, Meloen, R H, Oftung, F
We have previously demonstrated that the Mycobacterium leprae 18-kD heat shock protein (HSP18) is represented among the antigenic targets of human T cell responses induced by M. leprae immunization...