R. N. Jones, R. J. Hollis, S. A. Messer, D. J. Biedenbach, L. M. Deshp, ...
Strains of extended-spectrum �-lactamase-producing Klebsiella pneumoniae (ESBL-KP) have emerged worldwide. Concomitant ciprofloxacin resistance with ESBL production in K. pneumoniae isolates would...
Pfaller, M. A., Mujeeb, I., Hollis, R. J., Jones, R. N., Doern, G. V.
A total of 63 clinical isolates of Proteus mirabilis collected over a 19-month period were typed by the Dienes test and ribotyping. Ribotyping was performed using the fully automated RiboPrinter...
Pfaller, M. A., Diekema, D. J., Jones, R. N., Sader, H. S., Fluit, A. C., Hollis, R. J., ...
A surveillance program (SENTRY) of bloodstream infections (BSI) in the United States, Canada, Latin America, and Europe from 1997 through 1999 detected 1,184 episodes of candidemia in 71 medical...
Diekema, D. J., Pfaller, M. A., Messer, S. A., Houston, A., Hollis, R. J., Doern, G. V., ...
We compared the in vitro activity of BMS-207147, an investigational triazole, with those of itraconazole and fluconazole against 613 clinical bloodstream isolates of Candida spp. collected from...
Pfaller, M. A., Jones, R. N., Doern, G. V., Sader, H. S., Messer, S. A., Houston, A., ...
An international program of surveillance of bloodstream infections (BSI) in the United States, Canada, and Latin America detected 306 episodes of candidemia in 34 medical centers (22 in the United...
Yamazumi, T., Pfaller, M. A., Messer, S. A., Houston, A., Hollis, R. J., Jones, R. N.
The in vitro activities of the new triazole, ravuconazole (BMS-207147), were compared to those of fluconazole and itraconazole against 541 clinical isolates of Cryptococcus neoformans. Isolates were...
Pfaller, M. A., Messer, S. A., Hollis, R. J., Jones, R. N.
Posaconazole is a new investigational triazole with broad-spectrum antifungal activity. The in vitro activities of posaconazole were compared with those of itraconazole and fluconazole against 3,685...
Pfaller, M. A., Jones, R. N., Doern, G. V., Sader, H. S., Hollis, R. J., Messer, S. A., ...
An international program of surveillance of bloodstream infections (BSIs) in the United States, Canada, and South America between January and December 1997 detected 306 episodes of candidemia in 34...
Pfaller, M. A., Messer, S. A., Hollis, R. J., Jones, R. N., Doern, G. V., Brandt, M. E., ...
BMS-207147, Sch 56592, and voriconazole are three new investigational triazoles with broad-spectrum antifungal activity. The in vitro activities of these three agents were compared with those of...
Pfaller, M. A., Diekema, D. J., Jones, R. N., Messer, S. A., Hollis, R. J.
From 1 January 1997 through 31 December 2000, 2,047 bloodstream infections (BSIs) due to Candida spp. were reported from hospitals in the United States, Canada, Latin America, and Europe...
Pfaller, M. A., Messer, S. A., Hollis, R. J., Jones, R. N.
Posaconazole, ravuconazole, and voriconazole are new triazole derivatives that possess potent, broad-spectrum antifungal activity. We evaluated the in vitro activity of these investigational...
Pfaller, M. A., Messer, S. A., Hollis, R. J., Jones, R. N., Diekema, D. J.
The in vitro activities of ravuconazole and voriconazole were compared with those of amphotericin B, flucytosine (5FC), itraconazole, and fluconazole against 6,970 isolates of Candida spp. obtained...
Pfaller, M. A., Messer, S. A., Boyken, L., Huynh, H., Hollis, R. J., Diekema, D. J.
We determined the in vitro activity of flucytosine (5-fluorocytosine [5FC]) against 8,803 clinical isolates of Candida spp. (18 species) obtained from more than 200 medical centers worldwide between...
Pfaller, M. A., Diekema, D. J., Messer, S. A., Boyken, L., Huynh, H., Hollis, R. J.
A commercially prepared frozen broth microdilution panel (Trek Diagnostic Systems, Westlake, Ohio) was compared with a reference microdilution panel for antifungal susceptibility testing of two...
Pfaller, M. A., Diekema, D. J., Messer, S. A., Hollis, R. J., Jones, R. N.
Caspofungin is an echinocandin antifungal agent with broad-spectrum activity against Candida and Aspergillus spp. The in vitro activities of caspofungin against 3,959 isolates of Candida spp....
Maxwell, M. J., Messer, S. A., Hollis, R. J., Diekema, D. J., Pfaller, M. A.
The performance of the Etest for voriconazole and amphotericin B susceptibility testing of 162 isolates of Cryptococcus neoformans was assessed against the National Committee for Clinical Laboratory...
Yamazumi, T., Pfaller, M. A., Messer, S. A., Houston, A. K., Boyken, L., Hollis, R. J., ...
Strains of Cryptococcus neoformans expressing heteroresistance to fluconazole have been described previously. The present study was conducted to investigate the prevalence of heteroresistance among...
Pfaller, M. A., Diekema, D. J., Messer, S. A., Boyken, L., Hollis, R. J., Jones, R. N.
We determined the in vitro susceptibilities of 314 strains of Candida spp., representing 13 species rarely isolated from blood, to posaconazole and voriconazole as well as four licensed systemic...
Pfaller, J. B., Messer, S. A., Hollis, R. J., Diekema, D. J., Pfaller, M. A.
The performance of the Etest for voriconazole and for itraconazole susceptibility testing of 376 isolates of Aspergillus spp. was assessed in comparison with the National Committee for Clinical...
Maxwell, M. J., Messer, S. A., Hollis, R. J., Boyken, L., Tendolkar, S., Diekema, D. J., ...
The performance of Etest in fluconazole and voriconazole testing of 279 isolates of uncommon Candida spp. was assessed in comparison with the National Committee for Clinical Laboratory Standards...
Pfaller, M. A., Diekema, D. J., Messer, S. A., Boyken, L., Hollis, R. J.
The ARTEMIS Global Antifungal Susceptibility Program (ARTEMIS Program) was initiated in 2001 to provide focused surveillance of the activities of fluconazole and voriconazole against Candida spp....
Jones, R. N., Hollis, R. J., Messer, S. A., Biedenbach, D. J., Deshpande, L. M., ...
Strains of extended-spectrum β-lactamase-producing Klebsiella pneumoniae (ESBL-KP) have emerged worldwide. Concomitant ciprofloxacin resistance with ESBL production in K. pneumoniae isolates would...
Pfaller, M. A., Messer, S. A., Boyken, L., Tendolkar, S., Hollis, R. J., Diekema, D. J.
We examined the susceptibilities to fluconazole of 559 bloodstream infection isolates of Candida glabrata and grouped the isolates by patient age and geographic location within the United States....
Pfaller, M. A., Diekema, D. J., Boyken, L., Messer, S. A., Tendolkar, S., Hollis, R. J.
The performances of the Etest and the disk diffusion methods for testing of the susceptibilities of 235 Candida glabrata isolates to fluconazole and voriconazole were compared with that of the...
Garrison, M W, Anderson, D E, Campbell, D M, Carroll, K C, Malone, C L, Anderson, J D, ...
Emergence of Stenotrophomonas maltophilia as a nosocomial pathogen is becoming increasingly apparent. Pleiotropic resistance characterizes S. maltophilia. Furthermore, a slow growth rate and an...
Diekema, D. J., Messer, S. A., Hollis, R. J., Jones, R. N., Pfaller, M. A.
We examined the in vitro activity of caspofungin, posaconazole, voriconazole, ravuconazole, itraconazole, and amphotericin B against 448 recent clinical mold isolates. The endpoint for reading...
Voss, A, Pfaller, M A, Hollis, R J, Rhine-Chalberg, J, Doebbeling, B N
An apparent outbreak of serious Candida albicans infections (n = 6) occurred in a surgical intensive care unit over a 4-week period. Four patients developed C. albicans bloodstream infections. An...
Molecular epidemiology of an outbreak of meningococcal disease in a university community.
Edmond, M B, Hollis, R J, Houston, A K, Wenzel, R P
Over a 2-month period, five cases of serogroup C meningococcal disease occurred in Iowa City, Iowa. Two patients were unacquainted university students who had independently visited another university...
Strain variation among and antifungal susceptibilities of isolates of Candida krusei.
Berrouane, Y F, Hollis, R J, Pfaller, M A
Candida krusei is an emerging pathogen that is well known for its propensity to develop resistance to fluconazole and other azoles. Despite the potential clinical significance of C. krusei, little is...
In vitro susceptibility testing and DNA typing of Saccharomyces cerevisiae clinical isolates.
Zerva, L, Hollis, R J, Pfaller, M A
Saccharomyces spp. are widely distributed in nature and may colonize the normal human gastrointestinal tract. Although Saccharomyces cerevisiae isolates have been previously considered nonpathogenic,...
Evaluation and characterization of multiresistant Enterococcus faecium from 12 U.S. medical centers.
Sader, H S, Pfaller, M A, Tenover, F C, Hollis, R J, Jones, R N
Forty-two Enterococcus faecium isolates resistant to ampicillin, penicillin, gentamicin, streptomycin, vancomycin, and teicoplanin (VanA phenotype) from 12 U.S. medical centers were analyzed by...
Investigation of the sequence of colonization and candidemia in nonneutropenic patients.
Voss, A, Hollis, R J, Pfaller, M A, Wenzel, R P, Doebbeling, B N
Among neutropenic patients with hematologic malignancies, candidemia has been shown to arise typically from autoinfection after colonization. In patients without neutropenia, we examined the...
Reagan, D R, Pfaller, M A, Hollis, R J, Wenzel, R P
The objective of this hospital-based study was to determine the relationship between colonizing and infecting strains of Candida species and Torulopsis glabrata. Surveillance cultures from high-risk...
Restriction fragment analysis of a Candida tropicalis outbreak of sternal wound infections.
Doebbeling, B N, Hollis, R J, Isenberg, H D, Wenzel, R P, Pfaller, M A
An apparent single-source outbreak of Candida tropicalis sternal wound infections in eight patients was investigated by utilizing DNA restriction fragment analysis (RFA) with HindIII and BstNI. All...
Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., ...
We evaluated the NCCLS M44-P fluconazole disk diffusion method in comparison with the NCCLS M27-A2 broth microdilution method for determining the susceptibility of 276 isolates of Cryptococcus...
Pfaller, M. A., Boyken, L., Messer, S. A., Hollis, R. J., Diekema, D. J.
The shelf life of Mueller-Hinton agar supplemented with 2% glucose and methylene blue (0.5 μg/ml) (MH-GMB) prepared in the laboratory to test disk diffusion of voriconazole and fluconazole was...
Pfaller, M. A., Messer, S. A., Boyken, L., Tendolkar, S., Hollis, R. J., Diekema, D. J.
We examined the susceptibilities to amphotericin B, flucytosine, fluconazole, posaconazole, ravuconazole, voriconazole, and caspofungin of 601 invasive isolates of Candida glabrata and grouped the...
Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., ...
Cross-resistance within a class of antimicrobial agents is a problem that is often encountered with antibacterial agents, and it is also an issue with antifungal agents. A current example is...
Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., ...
The influence of test variables on in vitro susceptibility testing of caspofungin was examined with 694 isolates of Candida albicans including seven laboratory-derived glucan synthesis mutants. The...
Pfaller, M. A., Hazen, K. C., Messer, S. A., Boyken, L., Tendolkar, S., Hollis, R. J., ...
The accuracy of antifungal susceptibility tests is important for accurate resistance surveillance and for the clinical management of patients with serious infections. Our main objective was to...
Pfaller, M. A., Boyken, L., Messer, S. A., Tendolkar, S., Hollis, R. J., Diekema, D. J.
The performance of the Etest using Mueller-Hinton agar supplemented with glucose (2%) and methylene blue (0.5 μg/ml) (MH-GMB) for amphotericin B susceptibility testing of 4,936 isolates of Candida...
Activities of Available and Investigational Antifungal Agents against Rhodotorula Species
Diekema, D. J., Petroelje, B., Messer, S. A., Hollis, R. J., Pfaller, M. A
Rhodotorula species are emerging pathogens in immunocompromised patients. We report the in vitro activities of eight antifungals against 64 Rhodotorula isolates collected in surveillance programs...
Global Trends in the Antifungal Susceptibility of Cryptococcus neoformans (1990 to 2004)
Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., ...
The antifungal susceptibilities of 1,811 clinical isolates of Cryptococcus neoformans obtained from 100 laboratories in 5 geographic regions worldwide between 1990 and 2004 were determined. The MICs...
Pfaller, M. A., Boyken, L., Hollis, R. J., Messer, S. A., Tendolkar, S., Diekema, D. J.
The in vitro activity of itraconazole was determined against 7,299 isolates of Candida spp., 1,615 isolates of Cryptococcus neoformans, and 445 isolates of Aspergillus spp. obtained from over 200...
Pfaller, M. A., Boyken, L., Messer, S. A., Tendolkar, S., Hollis, R. J., Diekema, D. J.
The accuracy of antifungal susceptibility testing is important for reliable resistance surveillance and for the clinical management of patients with serious infections. Our primary objective was to...
Effectiveness of Anidulafungin in Eradicating Candida Species in Invasive Candidiasis
Pfaller, M. A., Diekema, D. J., Boyken, L., Messer, S. A., Tendolkar, S., Hollis, R. J., ...
In a phase 2 open-label, dose-ranging study in patients with candidemia, anidulafungin was effective in eradicating Candida albicans and other species of Candida. The anidulafungin MIC distribution...
Pfaller, M. A., Boyken, L., Hollis, R. J., Messer, S. A., Tendolkar, S., Diekema, D. J.
Anidulafungin is an echinocandin antifungal agent with potent activity against Candida spp. We assessed the in vitro activity of anidulafungin against 2,235 clinical isolates of Candida spp. using...
Messer, S. A., Diekema, D. J., Boyken, L., Tendolkar, S., Hollis, R. J., Pfaller, M. A.
Micafungin is a new echinocandin exhibiting broad-spectrum activity against Candida spp. The activity of the echinocandins against Candida species known to express intrinsic or acquired resistance to...
In Vitro Susceptibilities of Candida spp. to Caspofungin: Four Years of Global Surveillance
Pfaller, M. A., Boyken, L., Hollis, R. J., Messer, S. A., Tendolkar, S., Diekema, D. J.
Caspofungin is being used increasingly as therapy for invasive candidiasis. Prospective sentinel surveillance for emergence of in vitro resistance to caspofungin among invasive Candida spp. isolates...
Pfaller, M. A., Mujeeb, I., Hollis, R. J., Jones, R. N., Doern, G. V.
A total of 63 clinical isolates of Proteus mirabilis collected over a 19-month period were typed by the Dienes test and ribotyping. Ribotyping was performed using the fully automated RiboPrinter...
Pfaller, M. A., Diekema, D. J., Jones, R. N., Sader, H. S., Fluit, A. C., Hollis, R. J., ...
A surveillance program (SENTRY) of bloodstream infections (BSI) in the United States, Canada, Latin America, and Europe from 1997 through 1999 detected 1,184 episodes of candidemia in 71 medical...
Diekema, D. J., Pfaller, M. A., Messer, S. A., Houston, A., Hollis, R. J., Doern, G. V., ...
We compared the in vitro activity of BMS-207147, an investigational triazole, with those of itraconazole and fluconazole against 613 clinical bloodstream isolates of Candida spp. collected from...
Pfaller, M. A., Jones, R. N., Doern, G. V., Sader, H. S., Messer, S. A., Houston, A., ...
An international program of surveillance of bloodstream infections (BSI) in the United States, Canada, and Latin America detected 306 episodes of candidemia in 34 medical centers (22 in the United...
Yamazumi, T., Pfaller, M. A., Messer, S. A., Houston, A., Hollis, R. J., Jones, R. N.
The in vitro activities of the new triazole, ravuconazole (BMS-207147), were compared to those of fluconazole and itraconazole against 541 clinical isolates of Cryptococcus neoformans. Isolates were...
Pfaller, M. A., Messer, S. A., Hollis, R. J., Jones, R. N.
Posaconazole is a new investigational triazole with broad-spectrum antifungal activity. The in vitro activities of posaconazole were compared with those of itraconazole and fluconazole against 3,685...
Pfaller, M. A., Jones, R. N., Doern, G. V., Sader, H. S., Hollis, R. J., Messer, S. A., ...
An international program of surveillance of bloodstream infections (BSIs) in the United States, Canada, and South America between January and December 1997 detected 306 episodes of candidemia in 34...
Pfaller, M. A., Messer, S. A., Hollis, R. J., Jones, R. N., Doern, G. V., Brandt, M. E., ...
BMS-207147, Sch 56592, and voriconazole are three new investigational triazoles with broad-spectrum antifungal activity. The in vitro activities of these three agents were compared with those of...
Pfaller, M. A., Diekema, D. J., Jones, R. N., Messer, S. A., Hollis, R. J.
From 1 January 1997 through 31 December 2000, 2,047 bloodstream infections (BSIs) due to Candida spp. were reported from hospitals in the United States, Canada, Latin America, and Europe...
Pfaller, M. A., Messer, S. A., Hollis, R. J., Jones, R. N.
Posaconazole, ravuconazole, and voriconazole are new triazole derivatives that possess potent, broad-spectrum antifungal activity. We evaluated the in vitro activity of these investigational...
Pfaller, M. A., Messer, S. A., Hollis, R. J., Jones, R. N., Diekema, D. J.
The in vitro activities of ravuconazole and voriconazole were compared with those of amphotericin B, flucytosine (5FC), itraconazole, and fluconazole against 6,970 isolates of Candida spp. obtained...
Pfaller, M. A., Messer, S. A., Boyken, L., Huynh, H., Hollis, R. J., Diekema, D. J.
We determined the in vitro activity of flucytosine (5-fluorocytosine [5FC]) against 8,803 clinical isolates of Candida spp. (18 species) obtained from more than 200 medical centers worldwide between...
Pfaller, M. A., Diekema, D. J., Messer, S. A., Boyken, L., Huynh, H., Hollis, R. J.
A commercially prepared frozen broth microdilution panel (Trek Diagnostic Systems, Westlake, Ohio) was compared with a reference microdilution panel for antifungal susceptibility testing of two...
Pfaller, M. A., Diekema, D. J., Messer, S. A., Hollis, R. J., Jones, R. N.
Caspofungin is an echinocandin antifungal agent with broad-spectrum activity against Candida and Aspergillus spp. The in vitro activities of caspofungin against 3,959 isolates of Candida spp....
Maxwell, M. J., Messer, S. A., Hollis, R. J., Diekema, D. J., Pfaller, M. A.
The performance of the Etest for voriconazole and amphotericin B susceptibility testing of 162 isolates of Cryptococcus neoformans was assessed against the National Committee for Clinical Laboratory...
Yamazumi, T., Pfaller, M. A., Messer, S. A., Houston, A. K., Boyken, L., Hollis, R. J., ...
Strains of Cryptococcus neoformans expressing heteroresistance to fluconazole have been described previously. The present study was conducted to investigate the prevalence of heteroresistance among...
Pfaller, M. A., Diekema, D. J., Messer, S. A., Boyken, L., Hollis, R. J., Jones, R. N.
We determined the in vitro susceptibilities of 314 strains of Candida spp., representing 13 species rarely isolated from blood, to posaconazole and voriconazole as well as four licensed systemic...
Pfaller, J. B., Messer, S. A., Hollis, R. J., Diekema, D. J., Pfaller, M. A.
The performance of the Etest for voriconazole and for itraconazole susceptibility testing of 376 isolates of Aspergillus spp. was assessed in comparison with the National Committee for Clinical...
Maxwell, M. J., Messer, S. A., Hollis, R. J., Boyken, L., Tendolkar, S., Diekema, D. J., ...
The performance of Etest in fluconazole and voriconazole testing of 279 isolates of uncommon Candida spp. was assessed in comparison with the National Committee for Clinical Laboratory Standards...
Pfaller, M. A., Diekema, D. J., Messer, S. A., Boyken, L., Hollis, R. J.
The ARTEMIS Global Antifungal Susceptibility Program (ARTEMIS Program) was initiated in 2001 to provide focused surveillance of the activities of fluconazole and voriconazole against Candida spp....
Jones, R. N., Hollis, R. J., Messer, S. A., Biedenbach, D. J., Deshpande, L. M., ...
Strains of extended-spectrum β-lactamase-producing Klebsiella pneumoniae (ESBL-KP) have emerged worldwide. Concomitant ciprofloxacin resistance with ESBL production in K. pneumoniae isolates would...
Pfaller, M. A., Messer, S. A., Boyken, L., Tendolkar, S., Hollis, R. J., Diekema, D. J.
We examined the susceptibilities to fluconazole of 559 bloodstream infection isolates of Candida glabrata and grouped the isolates by patient age and geographic location within the United States....
Pfaller, M. A., Diekema, D. J., Boyken, L., Messer, S. A., Tendolkar, S., Hollis, R. J.
The performances of the Etest and the disk diffusion methods for testing of the susceptibilities of 235 Candida glabrata isolates to fluconazole and voriconazole were compared with that of the...
Garrison, M W, Anderson, D E, Campbell, D M, Carroll, K C, Malone, C L, Anderson, J D, ...
Emergence of Stenotrophomonas maltophilia as a nosocomial pathogen is becoming increasingly apparent. Pleiotropic resistance characterizes S. maltophilia. Furthermore, a slow growth rate and an...
Diekema, D. J., Messer, S. A., Hollis, R. J., Jones, R. N., Pfaller, M. A.
We examined the in vitro activity of caspofungin, posaconazole, voriconazole, ravuconazole, itraconazole, and amphotericin B against 448 recent clinical mold isolates. The endpoint for reading...
Voss, A, Pfaller, M A, Hollis, R J, Rhine-Chalberg, J, Doebbeling, B N
An apparent outbreak of serious Candida albicans infections (n = 6) occurred in a surgical intensive care unit over a 4-week period. Four patients developed C. albicans bloodstream infections. An...
Molecular epidemiology of an outbreak of meningococcal disease in a university community.
Edmond, M B, Hollis, R J, Houston, A K, Wenzel, R P
Over a 2-month period, five cases of serogroup C meningococcal disease occurred in Iowa City, Iowa. Two patients were unacquainted university students who had independently visited another university...
Strain variation among and antifungal susceptibilities of isolates of Candida krusei.
Berrouane, Y F, Hollis, R J, Pfaller, M A
Candida krusei is an emerging pathogen that is well known for its propensity to develop resistance to fluconazole and other azoles. Despite the potential clinical significance of C. krusei, little is...
In vitro susceptibility testing and DNA typing of Saccharomyces cerevisiae clinical isolates.
Zerva, L, Hollis, R J, Pfaller, M A
Saccharomyces spp. are widely distributed in nature and may colonize the normal human gastrointestinal tract. Although Saccharomyces cerevisiae isolates have been previously considered nonpathogenic,...
Evaluation and characterization of multiresistant Enterococcus faecium from 12 U.S. medical centers.
Sader, H S, Pfaller, M A, Tenover, F C, Hollis, R J, Jones, R N
Forty-two Enterococcus faecium isolates resistant to ampicillin, penicillin, gentamicin, streptomycin, vancomycin, and teicoplanin (VanA phenotype) from 12 U.S. medical centers were analyzed by...
Investigation of the sequence of colonization and candidemia in nonneutropenic patients.
Voss, A, Hollis, R J, Pfaller, M A, Wenzel, R P, Doebbeling, B N
Among neutropenic patients with hematologic malignancies, candidemia has been shown to arise typically from autoinfection after colonization. In patients without neutropenia, we examined the...
Reagan, D R, Pfaller, M A, Hollis, R J, Wenzel, R P
The objective of this hospital-based study was to determine the relationship between colonizing and infecting strains of Candida species and Torulopsis glabrata. Surveillance cultures from high-risk...
Restriction fragment analysis of a Candida tropicalis outbreak of sternal wound infections.
Doebbeling, B N, Hollis, R J, Isenberg, H D, Wenzel, R P, Pfaller, M A
An apparent single-source outbreak of Candida tropicalis sternal wound infections in eight patients was investigated by utilizing DNA restriction fragment analysis (RFA) with HindIII and BstNI. All...
Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., ...
We evaluated the NCCLS M44-P fluconazole disk diffusion method in comparison with the NCCLS M27-A2 broth microdilution method for determining the susceptibility of 276 isolates of Cryptococcus...
Pfaller, M. A., Boyken, L., Messer, S. A., Hollis, R. J., Diekema, D. J.
The shelf life of Mueller-Hinton agar supplemented with 2% glucose and methylene blue (0.5 μg/ml) (MH-GMB) prepared in the laboratory to test disk diffusion of voriconazole and fluconazole was...
Pfaller, M. A., Messer, S. A., Boyken, L., Tendolkar, S., Hollis, R. J., Diekema, D. J.
We examined the susceptibilities to amphotericin B, flucytosine, fluconazole, posaconazole, ravuconazole, voriconazole, and caspofungin of 601 invasive isolates of Candida glabrata and grouped the...
Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., ...
Cross-resistance within a class of antimicrobial agents is a problem that is often encountered with antibacterial agents, and it is also an issue with antifungal agents. A current example is...
Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., ...
The influence of test variables on in vitro susceptibility testing of caspofungin was examined with 694 isolates of Candida albicans including seven laboratory-derived glucan synthesis mutants. The...
Pfaller, M. A., Hazen, K. C., Messer, S. A., Boyken, L., Tendolkar, S., Hollis, R. J., ...
The accuracy of antifungal susceptibility tests is important for accurate resistance surveillance and for the clinical management of patients with serious infections. Our main objective was to...
Pfaller, M. A., Boyken, L., Messer, S. A., Tendolkar, S., Hollis, R. J., Diekema, D. J.
The performance of the Etest using Mueller-Hinton agar supplemented with glucose (2%) and methylene blue (0.5 μg/ml) (MH-GMB) for amphotericin B susceptibility testing of 4,936 isolates of Candida...
Activities of Available and Investigational Antifungal Agents against Rhodotorula Species
Diekema, D. J., Petroelje, B., Messer, S. A., Hollis, R. J., Pfaller, M. A
Rhodotorula species are emerging pathogens in immunocompromised patients. We report the in vitro activities of eight antifungals against 64 Rhodotorula isolates collected in surveillance programs...
Global Trends in the Antifungal Susceptibility of Cryptococcus neoformans (1990 to 2004)
Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., ...
The antifungal susceptibilities of 1,811 clinical isolates of Cryptococcus neoformans obtained from 100 laboratories in 5 geographic regions worldwide between 1990 and 2004 were determined. The MICs...
Pfaller, M. A., Boyken, L., Hollis, R. J., Messer, S. A., Tendolkar, S., Diekema, D. J.
The in vitro activity of itraconazole was determined against 7,299 isolates of Candida spp., 1,615 isolates of Cryptococcus neoformans, and 445 isolates of Aspergillus spp. obtained from over 200...
Pfaller, M. A., Boyken, L., Messer, S. A., Tendolkar, S., Hollis, R. J., Diekema, D. J.
The accuracy of antifungal susceptibility testing is important for reliable resistance surveillance and for the clinical management of patients with serious infections. Our primary objective was to...
Effectiveness of Anidulafungin in Eradicating Candida Species in Invasive Candidiasis
Pfaller, M. A., Diekema, D. J., Boyken, L., Messer, S. A., Tendolkar, S., Hollis, R. J., ...
In a phase 2 open-label, dose-ranging study in patients with candidemia, anidulafungin was effective in eradicating Candida albicans and other species of Candida. The anidulafungin MIC distribution...
Pfaller, M. A., Boyken, L., Hollis, R. J., Messer, S. A., Tendolkar, S., Diekema, D. J.
Anidulafungin is an echinocandin antifungal agent with potent activity against Candida spp. We assessed the in vitro activity of anidulafungin against 2,235 clinical isolates of Candida spp. using...
Messer, S. A., Diekema, D. J., Boyken, L., Tendolkar, S., Hollis, R. J., Pfaller, M. A.
Micafungin is a new echinocandin exhibiting broad-spectrum activity against Candida spp. The activity of the echinocandins against Candida species known to express intrinsic or acquired resistance to...
In Vitro Susceptibilities of Candida spp. to Caspofungin: Four Years of Global Surveillance
Pfaller, M. A., Boyken, L., Hollis, R. J., Messer, S. A., Tendolkar, S., Diekema, D. J.
Caspofungin is being used increasingly as therapy for invasive candidiasis. Prospective sentinel surveillance for emergence of in vitro resistance to caspofungin among invasive Candida spp. isolates...
Pfaller, M. A., Boyken, L., Hollis, R. J., Messer, S. A., Tendolkar, S., Diekema, D. J.
Micafungin is an echinocandin antifungal agent that has recently been approved for the prevention of invasive fungal infection and the treatment of esophageal candidiasis. Prospective sentinel...
Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., ...
Clinical laboratories frequently face the problem of delayed availability of commercially prepared approved reagents for performing susceptibility testing of new antimicrobials. Although this problem...
Pfaller, M. A., Boyken, L., Hollis, R. J., Kroeger, J., Messer, S. A., Tendolkar, S., ...
The echinocandins are being used increasingly as therapy for invasive candidiasis. Prospective sentinel surveillance for the emergence of in vitro resistance to the echinocandins among invasive...
Pfaller, M. A., Messer, S. A., Boyken, L., Tendolkar, S., Hollis, R. J., Diekema, D. J.
There are currently no FDA-approved broth microdilution antifungal susceptibility testing products or interpretive breakpoints for susceptibility testing of the new triazole posaconazole. Fluconazole...
Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., ...
Few data exist to describe in vitro patterns of cross-resistance among large collections of clinical Aspergillus isolates, including those of species other than Aspergillus fumigatus. We examined 771...
Pfaller, M. A., Boyken, L. B., Hollis, R. J., Kroeger, J., Messer, S. A., Tendolkar, S., ...
We performed 24- and 48-h MIC determinations and disk diffusion testing of fluconazole against more than 11,000 clinical isolates of Candida species. By using the reference MIC breakpoints, the...