Combined deficiency of factor V and factor VIII is due to mutations in either LMAN1 or MCFD2 (2006)
Zhang, B., McGee, B., Yamaoka, J.S., Guglielmone, H., Downes, K.A., Minoldo, S., ...
Mutations in LMAN1 (ERGIC-53) or MCFD2 cause combined deficiency of factor V and factor VIII (F5F8D). LMAN1 and MCFD2 form a protein complex that functions as a cargo receptor ferrying FV and FVIII...
Schröder, M., Clark, R., Liu, C. Y., Kaufman, R. J.
In Saccharomyces cerevisiae, splicing of HAC1 mRNA is initiated in response to the accumulation of unfolded proteins in the endoplasmic reticulum by the transmembrane kinase-endoribonuclease Ire1p....
GermOnline, a cross-species community knowledgebase on germ cell differentiation (2004)
Wiederkehr, C., Basavaraj, R., Hermida, L., Koch, R., Schlecht, U., ...
GermOnline provides information and microarray expression data for genes involved in mitosis and meiosis, gamete formation and germ line development across species. The database has been developed,...
GermOnline, a cross-species community knowledgebase on germ cell differentiation (2004)
Wiederkehr, C., Basavaraj, R., Hermida, L., Koch, R., Schlecht, U., ...
GermOnline provides information and microarray expression data for genes involved in mitosis and meiosis, gamete formation and germ line development across species. The database has been developed,...
Schröder, M., Clark, R., Kaufman, R. J.
The unfolded protein response (UPR) is a signalling pathway leading to transcriptional activation of genes that protect cells from accumulation of unfolded proteins in the lumen of the endoplasmic...
Bleeding due to disruption of a cargo-specific ER-to-Golgi transport complex (2003)
Zhang, B., Cunningham, M.A., Nichols, W.C., Bernat, J.A., Seligsohn, U., Pipe, S.W., ...
Mutations in LMAN1 (also called ERGIC-53) result in combined deficiency of factor V and factor VIII (F5F8D), an autosomal recessive bleeding disorder characterized by coordinate reduction of both...
Proteolytic maturation of protein C upon engineering the mouse mammary gland to express furin.
Drews, R, Paleyanda, R K, Lee, T K, Chang, R R, Rehemtulla, A, Kaufman, R J, ...
Endoproteolytic processing of the human protein C (HPC) precursor to its mature form involves cleavage of the propeptide after amino acids Lys-2-Arg-1 and removal of a Lys156-Arg157 dipeptide...
TAR RNA-binding protein is an inhibitor of the interferon-induced protein kinase PKR.
Park, H, Davies, M V, Langland, J O, Chang, H W, Nam, Y S, Tartaglia, J, ...
A cDNA encoding a double-stranded-RNA (dsRNA)-binding protein was isolated by screening a HeLa cell cDNA expression library for proteins that bind the HIV-1 Rev-responsive-element RNA. The cDNA...
A chimeric gene was constructed encoding the entire murine dihydrofolate reductase (DHFR) protein with a carboxyl-terminal extension encompassing amino acids 494-795 of the rat glucocorticoid...
Rehemtulla, A, Roth, D A, Wasley, L C, Kuliopulos, A, Walsh, C T, Furie, B, ...
Coagulation factor IX is a serine protease for which high-level expression of biologically active protein in heterologous cells is limited due to inefficient proteolytic removal of the propeptide as...
Expression of bovine vitamin K-dependent carboxylase activity in baculovirus-infected insect cells.
Roth, D A, Rehemtulla, A, Kaufman, R J, Walsh, C T, Furie, B, Furie, B C
A vitamin K-dependent carboxylase has recently been purified from bovine liver microsomes and candidate cDNA clones have been isolated. Definitive identification of the carboxylase remains...
Rehemtulla, A, Dorner, A J, Kaufman, R J
PACE (paired basic amino acid cleaving enzyme) is a subtilisin-like serine protease involved in processing of propeptides in the constitutive secretory pathway. We here demonstrate that the...
Wood, C R, Morris, G E, Alderman, E M, Fouser, L, Kaufman, R J
The encephalomyocarditis virus (EMCV) leader sequence is responsible for efficient, cap-independent translation initiation from the viral RNA. It has been used to increase the expression of internal...
Protein dissociation from GRP78 and secretion are blocked by depletion of cellular ATP levels.
Dorner, A J, Wasley, L C, Kaufman, R J
Secretory proteins expressed in Chinese hamster ovary (CHO) cells interact to various degrees with glucose-regulated protein 78 (GRP78), a resident protein of the endoplasmic reticulum. von...
Wise, R J, Barr, P J, Wong, P A, Kiefer, M C, Brake, A J, Kaufman, R J
Intracellular proteolytic processing of precursor polypeptides is an essential step in the maturation of many proteins, including plasma proteins, hormones, neuropeptides, and growth factors. Most...
Davies, M V, Chang, H W, Jacobs, B L, Kaufman, R J
Vaccinia virus has evolved multiple mechanisms to counteract the interferon-induced antiviral host cell response. Recently, two vaccinia virus gene products were shown to interfere with the activity...
Jang, S K, Davies, M V, Kaufman, R J, Wimmer, E
Expression vectors that yield mono-, di-, and tricistronic mRNAs upon transfection of COS-1 cells were used to assess the influence of the 5' nontranslated regions (5'NTRs) on translation of reporter...
Dorner, A J, Bonneville, F, Kriz, R, Kelleher, K, Bean, K, Kaufman, R J
We report here the nucleotide sequence of a full-length Chinese hamster genomic proviral element, CHIAP34. CHIAP34 is 6,403 bp long with long terminal repeats of 311 bp at each end. The genetic...
Proteolytic requirements for thrombin activation of anti-hemophilic factor (factor VIII).
Factor VIII functions in the intrinsic pathway of coagulation as the cofactor for factor IXa proteolytic activation of factor X. Proteolytic cleavage is required for activation and may be responsible...
Translation initiation on poliovirus and encephalomyocarditis virus (EMCV) mRNAs occurs by a cap-independent mechanism utilizing an internal ribosomal entry site (IRES). However, no unifying...
Davies, M V, Elroy-Stein, O, Jagus, R, Moss, B, Kaufman, R J
Interferon resistance of vaccinia virus is mediated by specific inhibition of phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF-2 alpha) by the double-stranded-RNA-activated...
Davies, M V, Furtado, M, Hershey, J W, Thimmappaya, B, Kaufman, R J
Adenovirus VA RNAs (virus-associated RNAs) are small polymerase III transcripts that are required for efficient initiation of mRNA translation late in adenovirus infection. VAI RNA prevents...
Expression of a synthetic bovine rhodopsin gene in monkey kidney cells.
Oprian, D D, Molday, R S, Kaufman, R J, Khorana, H G
We report here the high-level expression of a synthetic gene for bovine rhodopsin in transfected monkey kidney COS-1 cells. Rhodopsin is produced in these cells to a level of 0.3% of the cell...
Complete cDNA and derived amino acid sequence of human factor V.
Jenny, R J, Pittman, D D, Toole, J J, Kriz, R W, Aldape, R A, Hewick, R M, ...
cDNA clones encoding human factor V have been isolated from an oligo(dT)-primed human fetal liver cDNA library prepared with vector Charon 21A. The cDNA sequence of factor V from three overlapping...
GermOnline, a cross-species community knowledgebase on germ cell differentiation
Wiederkehr, C., Basavaraj, R., Hermida, L., Koch, R., Schlecht, U., ...
GermOnline provides information and microarray expression data for genes involved in mitosis and meiosis, gamete formation and germ line development across species. The database has been developed,...
A conditional glucocorticoid-responsive expression vector system is described for highly inducible expression of heterologous genes in mammalian cells. This host-vector system requires high level...
Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells.
Kaufman, R J, Murtha, P, Ingolia, D E, Yeung, C Y, Kellems, R E
We demonstrate that an adenosine deaminase (ADA) cDNA gene can function as a dominant selectable and amplifiable marker for gene transfer experiments in mammalian cells. Cells that incorporate the...
Kaufman, R J, Davies, M V, Wasley, L C, Michnick, D
Dicistronic mRNA expression vectors efficiently translate a 5' open reading frame (ORF) and contain a selectable marker within the 3' end which is inefficiently translated. In these vectors, the...
Clark, S C, Arya, S K, Wong-Staal, F, Matsumoto-Kobayashi, M, Kay, R M, Kaufman, R J, ...
The partial amino acid sequences of human T-cell growth factors (TCGFs) isolated from normal peripheral blood lymphocytes and from a leukemia T-cell line (Jurkat) show that the amino-terminal...
Chang, A C, Erlich, H A, Gunsalus, R P, Nunberg, J H, Kaufman, R J, Schimke, R T, ...
Plasmids containing a mouse cDNA sequence encoding the enzyme dihydrofolate reductase (DHFR; tetrahydrofolate dehydrogenase; 5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3) have been used...
Ramaiah, K V, Davies, M V, Chen, J J, Kaufman, R J
The inhibition of protein synthesis that occurs upon phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF-2 alpha) at serine 51 correlates with reduced guanine nucleotide...
Kaufman, R J, Davies, M V, Pathak, V K, Hershey, J W
Phosphorylation of the alpha subunit of the eucaryotic translation initiation factor (eIF-2 alpha) by the double-stranded RNA-activated inhibitor (DAI) kinase correlates with inhibition of...
Kaufman, R J, Wasley, L C, Davies, M V, Wise, R J, Israel, D I, Dorner, A J
In plasma, antihemophilic factor (factor VIII) exists as a 200-kilodalton heavy-chain polypeptide in a metal ion association with an 80-kilodalton light-chain polypeptide. This complex is bound by...
A cloned human CCAAT-box-binding factor stimulates transcription from the human hsp70 promoter.
Lum, L S, Sultzman, L A, Kaufman, R J, Linzer, D I, Wu, B J
The basal promoter of the human hsp70 gene is predominantly controlled by a CCAAT element at position -70 relative to the transcriptional initiation site. We report the isolation of a novel cDNA...
The translational efficiency of mRNA molecules transcribed from plasmid DNA transfected into COS-1 monkey cells can be increased 10- to 20-fold by the coexpression of the adenovirus virus-associated...
Reduction of endogenous GRP78 levels improves secretion of a heterologous protein in CHO cells.
Dorner, A J, Krane, M G, Kaufman, R J
GRP78 is localized in the endoplasmic reticulum and associates with improperly folded or underglycosylated proteins. The role of GRP78 in secretion was studied in Chinese hamster ovary cells...
Kaufman, R J, Wasley, L C, Spiliotes, A J, Gossels, S D, Latt, S A, Larsen, G R, ...
Expression of human tissue-type plasminogen activator (t-PA) at high levels has been achieved in Chinese hamster ovary (CHO) cells by cotransfection and subsequent coamplification of the transfected...
Murray, M J, Kaufman, R J, Latt, S A, Weinberg, R A
The transcriptional promoter of the Harvey sarcoma virus long terminal repeat has been used to construct a biologically active dihydrofolate reductase chimera. The construction placed the long...
Kaufman, R J, Sharp, P A, Latt, S A
A modular dihydrofolate reductase gene has been introduced into Chinese hamster ovary cells lacking dihydrofolate reductase. Clones capable of growth in the absence of added nucleosides contain one...
Kingston, R E, Kaufman, R J, Sharp, P A
During adenovirus infection, the EII promoter is positively regulated by products of the EIa region. We have studied this regulation by fusing a DNA segment containing the adenovirus EII promoter to...
Amplification and loss of dihydrofolate reductase genes in a Chinese hamster ovary cell line.
During stepwise increases in the methotrexate concentration in culture medium, we selected Chinese hamster ovary cells that contained elevated dihydrofolate reductase levels which were proportional...
Loss and stabilization of amplified dihydrofolate reductase genes in mouse sarcoma S-180 cell lines.
Kaufman, R J, Brown, P C, Schimke, R T
We studied the loss and stabilization of dihydrofolate reductase genes in clones of a methotrexate-resistant murine S-180 cell line. These cells contained multiple copies of the dihydrofolate...
Dihydrofolate reductase (DHFR) modular genes have been constructed with segments containing the adenovirus major late promoter, a 3' splice site from a variable region immunoglobulin gene, a DHFR...
Growth-dependent expression of dihydrofolate reductase mRNA from modular cDNA genes.
Dihydrofolate reductase (DHFR) synthesis is regulated in a growth-dependent fashion. Dividing cells synthesize DHFR at a 10-fold-higher rate than do stationary cells. To study this growth-dependent...
Toole, J J, Pittman, D D, Orr, E C, Murtha, P, Wasley, L C, Kaufman, R J
Factor VIII (antihemophilic factor) is a high molecular weight plasma glycoprotein that participates in the blood clotting cascade. The recent cloning and sequence analysis of the cDNA encoding human...
Nunberg, J H, Kaufman, R J, Schimke, R T, Urlaub, G, Chasin, L A
Methotrexate-resistant Chinese hamster ovary cells selected for high resistance by progressive increments of methotrexate in the culture medium have levels of dihydrofolate reductase...
A transient expression system was used to study the role of the adenovirus late and simian virus 40 (SV40) early mRNA leader sequences and adenovirus virus-associated (VA) RNAs in mRNA translation....
Kaufman, R J, Brown, P C, Schimke, R T
Selection of mammalian cells in progressively increasing concentrations of methotrexate results in selective amplification of DNA sequences coding for dihydrofolate reductase (tetrahydrofolate...
Linkage of combined factors V and VIII deficiency to chromosome 18q by homozygosity mapping.
Nichols, W C, Seligsohn, U, Zivelin, A, Terry, V H, Arnold, N D, Siemieniak, D R, ...
Combined Factors V and VIII deficiency is an autosomal recessive bleeding disorder identified in at least 58 families comprising a number of different ethnic groups. Affected patients present with a...
Intracellular trafficking of factor VIII to von Willebrand factor storage granules.
Rosenberg, J B, Foster, P A, Kaufman, R J, Vokac, E A, Moussalli, M, Kroner, P A, ...
In plasma, von Willebrand factor (vWf) associates with Factor VIII (FVIII); however, the site at which these proteins first interact has not been defined. Administration of 1-desamino-8-D-arginine...
Milburn, S C, Hershey, J W, Davies, M V, Kelleher, K, Kaufman, R J
Eukaryotic protein synthesis initiation factor 4B (eIF-4B) is an 80,000 dalton polypeptide which is essential for the binding of mRNA to ribosomes. A highly purified preparation of eIF-4B from HeLa...
Kaufman, R J, Murtha, P, Davies, M V
The translation of polycistronic mRNAs in mammalian cells was studied. Transcription units, constructed to contain one, two or three open reading frames (ORFs), were introduced stably into Chinese...
Dorner, A J, Wasley, L C, Kaufman, R J
GRP78 is a resident protein of the endoplasmic reticulum (ER) and a member of the glucose regulated protein (GRP) family. Many secretion incompetent proteins are found in stable association with...
Proteolytic maturation of protein C upon engineering the mouse mammary gland to express furin.
Drews, R, Paleyanda, R K, Lee, T K, Chang, R R, Rehemtulla, A, Kaufman, R J, ...
Endoproteolytic processing of the human protein C (HPC) precursor to its mature form involves cleavage of the propeptide after amino acids Lys-2-Arg-1 and removal of a Lys156-Arg157 dipeptide...
TAR RNA-binding protein is an inhibitor of the interferon-induced protein kinase PKR.
Park, H, Davies, M V, Langland, J O, Chang, H W, Nam, Y S, Tartaglia, J, ...
A cDNA encoding a double-stranded-RNA (dsRNA)-binding protein was isolated by screening a HeLa cell cDNA expression library for proteins that bind the HIV-1 Rev-responsive-element RNA. The cDNA...
A chimeric gene was constructed encoding the entire murine dihydrofolate reductase (DHFR) protein with a carboxyl-terminal extension encompassing amino acids 494-795 of the rat glucocorticoid...
Rehemtulla, A, Roth, D A, Wasley, L C, Kuliopulos, A, Walsh, C T, Furie, B, ...
Coagulation factor IX is a serine protease for which high-level expression of biologically active protein in heterologous cells is limited due to inefficient proteolytic removal of the propeptide as...
Expression of bovine vitamin K-dependent carboxylase activity in baculovirus-infected insect cells.
Roth, D A, Rehemtulla, A, Kaufman, R J, Walsh, C T, Furie, B, Furie, B C
A vitamin K-dependent carboxylase has recently been purified from bovine liver microsomes and candidate cDNA clones have been isolated. Definitive identification of the carboxylase remains...
Rehemtulla, A, Dorner, A J, Kaufman, R J
PACE (paired basic amino acid cleaving enzyme) is a subtilisin-like serine protease involved in processing of propeptides in the constitutive secretory pathway. We here demonstrate that the...
Wood, C R, Morris, G E, Alderman, E M, Fouser, L, Kaufman, R J
The encephalomyocarditis virus (EMCV) leader sequence is responsible for efficient, cap-independent translation initiation from the viral RNA. It has been used to increase the expression of internal...
Protein dissociation from GRP78 and secretion are blocked by depletion of cellular ATP levels.
Dorner, A J, Wasley, L C, Kaufman, R J
Secretory proteins expressed in Chinese hamster ovary (CHO) cells interact to various degrees with glucose-regulated protein 78 (GRP78), a resident protein of the endoplasmic reticulum. von...
Wise, R J, Barr, P J, Wong, P A, Kiefer, M C, Brake, A J, Kaufman, R J
Intracellular proteolytic processing of precursor polypeptides is an essential step in the maturation of many proteins, including plasma proteins, hormones, neuropeptides, and growth factors. Most...
Davies, M V, Chang, H W, Jacobs, B L, Kaufman, R J
Vaccinia virus has evolved multiple mechanisms to counteract the interferon-induced antiviral host cell response. Recently, two vaccinia virus gene products were shown to interfere with the activity...
Jang, S K, Davies, M V, Kaufman, R J, Wimmer, E
Expression vectors that yield mono-, di-, and tricistronic mRNAs upon transfection of COS-1 cells were used to assess the influence of the 5' nontranslated regions (5'NTRs) on translation of reporter...
Dorner, A J, Bonneville, F, Kriz, R, Kelleher, K, Bean, K, Kaufman, R J
We report here the nucleotide sequence of a full-length Chinese hamster genomic proviral element, CHIAP34. CHIAP34 is 6,403 bp long with long terminal repeats of 311 bp at each end. The genetic...
Proteolytic requirements for thrombin activation of anti-hemophilic factor (factor VIII).
Factor VIII functions in the intrinsic pathway of coagulation as the cofactor for factor IXa proteolytic activation of factor X. Proteolytic cleavage is required for activation and may be responsible...
Translation initiation on poliovirus and encephalomyocarditis virus (EMCV) mRNAs occurs by a cap-independent mechanism utilizing an internal ribosomal entry site (IRES). However, no unifying...
Davies, M V, Elroy-Stein, O, Jagus, R, Moss, B, Kaufman, R J
Interferon resistance of vaccinia virus is mediated by specific inhibition of phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF-2 alpha) by the double-stranded-RNA-activated...
Davies, M V, Furtado, M, Hershey, J W, Thimmappaya, B, Kaufman, R J
Adenovirus VA RNAs (virus-associated RNAs) are small polymerase III transcripts that are required for efficient initiation of mRNA translation late in adenovirus infection. VAI RNA prevents...
Expression of a synthetic bovine rhodopsin gene in monkey kidney cells.
Oprian, D D, Molday, R S, Kaufman, R J, Khorana, H G
We report here the high-level expression of a synthetic gene for bovine rhodopsin in transfected monkey kidney COS-1 cells. Rhodopsin is produced in these cells to a level of 0.3% of the cell...
Complete cDNA and derived amino acid sequence of human factor V.
Jenny, R J, Pittman, D D, Toole, J J, Kriz, R W, Aldape, R A, Hewick, R M, ...
cDNA clones encoding human factor V have been isolated from an oligo(dT)-primed human fetal liver cDNA library prepared with vector Charon 21A. The cDNA sequence of factor V from three overlapping...
GermOnline, a cross-species community knowledgebase on germ cell differentiation
Wiederkehr, C., Basavaraj, R., Hermida, L., Koch, R., Schlecht, U., ...
GermOnline provides information and microarray expression data for genes involved in mitosis and meiosis, gamete formation and germ line development across species. The database has been developed,...
A conditional glucocorticoid-responsive expression vector system is described for highly inducible expression of heterologous genes in mammalian cells. This host-vector system requires high level...
Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells.
Kaufman, R J, Murtha, P, Ingolia, D E, Yeung, C Y, Kellems, R E
We demonstrate that an adenosine deaminase (ADA) cDNA gene can function as a dominant selectable and amplifiable marker for gene transfer experiments in mammalian cells. Cells that incorporate the...
Kaufman, R J, Davies, M V, Wasley, L C, Michnick, D
Dicistronic mRNA expression vectors efficiently translate a 5' open reading frame (ORF) and contain a selectable marker within the 3' end which is inefficiently translated. In these vectors, the...
Clark, S C, Arya, S K, Wong-Staal, F, Matsumoto-Kobayashi, M, Kay, R M, Kaufman, R J, ...
The partial amino acid sequences of human T-cell growth factors (TCGFs) isolated from normal peripheral blood lymphocytes and from a leukemia T-cell line (Jurkat) show that the amino-terminal...
Chang, A C, Erlich, H A, Gunsalus, R P, Nunberg, J H, Kaufman, R J, Schimke, R T, ...
Plasmids containing a mouse cDNA sequence encoding the enzyme dihydrofolate reductase (DHFR; tetrahydrofolate dehydrogenase; 5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3) have been used...
Ramaiah, K V, Davies, M V, Chen, J J, Kaufman, R J
The inhibition of protein synthesis that occurs upon phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF-2 alpha) at serine 51 correlates with reduced guanine nucleotide...
Kaufman, R J, Davies, M V, Pathak, V K, Hershey, J W
Phosphorylation of the alpha subunit of the eucaryotic translation initiation factor (eIF-2 alpha) by the double-stranded RNA-activated inhibitor (DAI) kinase correlates with inhibition of...
Kaufman, R J, Wasley, L C, Davies, M V, Wise, R J, Israel, D I, Dorner, A J
In plasma, antihemophilic factor (factor VIII) exists as a 200-kilodalton heavy-chain polypeptide in a metal ion association with an 80-kilodalton light-chain polypeptide. This complex is bound by...
A cloned human CCAAT-box-binding factor stimulates transcription from the human hsp70 promoter.
Lum, L S, Sultzman, L A, Kaufman, R J, Linzer, D I, Wu, B J
The basal promoter of the human hsp70 gene is predominantly controlled by a CCAAT element at position -70 relative to the transcriptional initiation site. We report the isolation of a novel cDNA...
The translational efficiency of mRNA molecules transcribed from plasmid DNA transfected into COS-1 monkey cells can be increased 10- to 20-fold by the coexpression of the adenovirus virus-associated...
Reduction of endogenous GRP78 levels improves secretion of a heterologous protein in CHO cells.
Dorner, A J, Krane, M G, Kaufman, R J
GRP78 is localized in the endoplasmic reticulum and associates with improperly folded or underglycosylated proteins. The role of GRP78 in secretion was studied in Chinese hamster ovary cells...
Kaufman, R J, Wasley, L C, Spiliotes, A J, Gossels, S D, Latt, S A, Larsen, G R, ...
Expression of human tissue-type plasminogen activator (t-PA) at high levels has been achieved in Chinese hamster ovary (CHO) cells by cotransfection and subsequent coamplification of the transfected...
Murray, M J, Kaufman, R J, Latt, S A, Weinberg, R A
The transcriptional promoter of the Harvey sarcoma virus long terminal repeat has been used to construct a biologically active dihydrofolate reductase chimera. The construction placed the long...
Kaufman, R J, Sharp, P A, Latt, S A
A modular dihydrofolate reductase gene has been introduced into Chinese hamster ovary cells lacking dihydrofolate reductase. Clones capable of growth in the absence of added nucleosides contain one...
Kingston, R E, Kaufman, R J, Sharp, P A
During adenovirus infection, the EII promoter is positively regulated by products of the EIa region. We have studied this regulation by fusing a DNA segment containing the adenovirus EII promoter to...
Amplification and loss of dihydrofolate reductase genes in a Chinese hamster ovary cell line.
During stepwise increases in the methotrexate concentration in culture medium, we selected Chinese hamster ovary cells that contained elevated dihydrofolate reductase levels which were proportional...
Loss and stabilization of amplified dihydrofolate reductase genes in mouse sarcoma S-180 cell lines.
Kaufman, R J, Brown, P C, Schimke, R T
We studied the loss and stabilization of dihydrofolate reductase genes in clones of a methotrexate-resistant murine S-180 cell line. These cells contained multiple copies of the dihydrofolate...
Dihydrofolate reductase (DHFR) modular genes have been constructed with segments containing the adenovirus major late promoter, a 3' splice site from a variable region immunoglobulin gene, a DHFR...
Growth-dependent expression of dihydrofolate reductase mRNA from modular cDNA genes.
Dihydrofolate reductase (DHFR) synthesis is regulated in a growth-dependent fashion. Dividing cells synthesize DHFR at a 10-fold-higher rate than do stationary cells. To study this growth-dependent...
Toole, J J, Pittman, D D, Orr, E C, Murtha, P, Wasley, L C, Kaufman, R J
Factor VIII (antihemophilic factor) is a high molecular weight plasma glycoprotein that participates in the blood clotting cascade. The recent cloning and sequence analysis of the cDNA encoding human...
Nunberg, J H, Kaufman, R J, Schimke, R T, Urlaub, G, Chasin, L A
Methotrexate-resistant Chinese hamster ovary cells selected for high resistance by progressive increments of methotrexate in the culture medium have levels of dihydrofolate reductase...
A transient expression system was used to study the role of the adenovirus late and simian virus 40 (SV40) early mRNA leader sequences and adenovirus virus-associated (VA) RNAs in mRNA translation....
Kaufman, R J, Brown, P C, Schimke, R T
Selection of mammalian cells in progressively increasing concentrations of methotrexate results in selective amplification of DNA sequences coding for dihydrofolate reductase (tetrahydrofolate...
Linkage of combined factors V and VIII deficiency to chromosome 18q by homozygosity mapping.
Nichols, W C, Seligsohn, U, Zivelin, A, Terry, V H, Arnold, N D, Siemieniak, D R, ...
Combined Factors V and VIII deficiency is an autosomal recessive bleeding disorder identified in at least 58 families comprising a number of different ethnic groups. Affected patients present with a...
Intracellular trafficking of factor VIII to von Willebrand factor storage granules.
Rosenberg, J B, Foster, P A, Kaufman, R J, Vokac, E A, Moussalli, M, Kroner, P A, ...
In plasma, von Willebrand factor (vWf) associates with Factor VIII (FVIII); however, the site at which these proteins first interact has not been defined. Administration of 1-desamino-8-D-arginine...
Milburn, S C, Hershey, J W, Davies, M V, Kelleher, K, Kaufman, R J
Eukaryotic protein synthesis initiation factor 4B (eIF-4B) is an 80,000 dalton polypeptide which is essential for the binding of mRNA to ribosomes. A highly purified preparation of eIF-4B from HeLa...
Kaufman, R J, Murtha, P, Davies, M V
The translation of polycistronic mRNAs in mammalian cells was studied. Transcription units, constructed to contain one, two or three open reading frames (ORFs), were introduced stably into Chinese...
Dorner, A J, Wasley, L C, Kaufman, R J
GRP78 is a resident protein of the endoplasmic reticulum (ER) and a member of the glucose regulated protein (GRP) family. Many secretion incompetent proteins are found in stable association with...