R. K. Banerjee

Iodide modulation of the EDTA-induced iodine reductase activity of horseradish peroxidase by interaction at or near the EDTA-binding site.

Bhattacharyya, D K, Bandyopadhyay, U, Chatterjee, R, Banerjee, R K

Horseradish peroxidase (HRP) catalyses the reduction of iodinium ion (I+) to iodide by H2O2 in the presence of EDTA. I+ reduction occurs optimally at pH 6 whereas the enzyme catalyses iodide...

Localization of gastric peroxidase and its inhibition by mercaptomethylimidazole, an inducer of gastric acid secretion.

Bandyopadhyay, U, Bhattacharyya, D K, Chatterjee, R, Banerjee, R K

Mercaptomethylimidazole (MMI) is a potent inducer of gastric acid secretion which is associated with significant inhibition of peroxidase activity of rat gastric mucosa in vivo. A time-dependent...

Thiocyanate, a plausible physiological electron donor of gastric peroxidase.

Das, D, De, P K, Banerjee, R K

Gastric peroxidase (GPO) was purified to apparent homogeneity to characterize its major physiological electron donor. The enzyme (RZ = 0.7), with a subunit molecular mass of 50 kDa, is a...

Irreversible inactivation of lactoperoxidase by mercaptomethylimidazole through generation of a thiyl radical: its use as a probe to study the active site.

Bandyopadhyay, U, Bhattacharyya, D K, Chatterjee, R, Banerjee, R K

The mechanism of suicidal inactivation of lactoperoxidase (LPO) by mercaptomethylimidazole (MMI) has been studied. Analogue studies indicate a specific requirement for the thiol group of MMI for...

Mechanism-based inactivation of gastric peroxidase by mercaptomethylimidazole.

Bandyopadhyay, U, Bhattacharyya, D K, Banerjee, R K

The mechanism of inhibition of gastric peroxidase (GPO) activity by mercaptomethylimidazole (MMI), an inducer of gastric acid secretion, has been investigated. Incubation of purified GPO with MMI in...

Mechanism of inhibition of horseradish peroxidase-catalysed iodide oxidation by EDTA.

Bhattacharyya, D K, Adak, S, Bandyopadhyay, U, Banerjee, R K

EDTA inhibits horseradish peroxidase (HRP)-catalysed iodide oxidation in a concentration and pH-dependent manner. It is more effective at pH 6 than at lower pH values. A plot of log Kiapp. values as...

Characterization of sheep lacrimal-gland peroxidase and its major physiological electron donor.

Mazumdar, A, Chatterjee, R, Adak, S, Ghosh, A, Mondal, C, Banerjee, R K

A soluble sheep lacrimal-gland peroxidase was purified to apparent homogeneity. It had a native molecular mass of 75 kDa with a subunit molecular mass of 82 kDa and an isoelectric point of 6.5....

Probing the active site residues in aromatic donor oxidation in horseradish peroxidase: involvement of an arginine and a tyrosine residue in aromatic donor binding.

Adak, S, Mazumder, A, Banerjee, R K

The plausible role of arginine and tyrosine residues at the active side of horseradish peroxidase (HRP) in aromatic donor (guaiacol) oxidation was probed by chemical modification followed by...

Mechanism-based inactivation of lacrimal-gland peroxidase by phenylhydrazine: a suicidal substrate to probe the active site.

Mazumdar, A, Adak, S, Chatterjee, R, Banerjee, R K

Humans are exposed to various hydrazine derivatives for therapeutic control of several diseases, and mammalian peroxidases are implicated in the oxidative metabolism of many drugs. The results...

Haem propionates control oxidative and reductive activities of horseradish peroxidase by maintaining the correct orientation of the haem.

Adak, S, Banerjee, R K

The role of haem propionates in oxidative and reductive reactions catalysed by horseradish peroxidase (HRP) was studied after successful reconstitution of ferric protoporphyrin IX dimethyl ester...

Iodide modulation of the EDTA-induced iodine reductase activity of horseradish peroxidase by interaction at or near the EDTA-binding site.

Bhattacharyya, D K, Bandyopadhyay, U, Chatterjee, R, Banerjee, R K

Horseradish peroxidase (HRP) catalyses the reduction of iodinium ion (I+) to iodide by H2O2 in the presence of EDTA. I+ reduction occurs optimally at pH 6 whereas the enzyme catalyses iodide...

Localization of gastric peroxidase and its inhibition by mercaptomethylimidazole, an inducer of gastric acid secretion.

Bandyopadhyay, U, Bhattacharyya, D K, Chatterjee, R, Banerjee, R K

Mercaptomethylimidazole (MMI) is a potent inducer of gastric acid secretion which is associated with significant inhibition of peroxidase activity of rat gastric mucosa in vivo. A time-dependent...

Thiocyanate, a plausible physiological electron donor of gastric peroxidase.

Das, D, De, P K, Banerjee, R K

Gastric peroxidase (GPO) was purified to apparent homogeneity to characterize its major physiological electron donor. The enzyme (RZ = 0.7), with a subunit molecular mass of 50 kDa, is a...

Irreversible inactivation of lactoperoxidase by mercaptomethylimidazole through generation of a thiyl radical: its use as a probe to study the active site.

Bandyopadhyay, U, Bhattacharyya, D K, Chatterjee, R, Banerjee, R K

The mechanism of suicidal inactivation of lactoperoxidase (LPO) by mercaptomethylimidazole (MMI) has been studied. Analogue studies indicate a specific requirement for the thiol group of MMI for...

Mechanism-based inactivation of gastric peroxidase by mercaptomethylimidazole.

Bandyopadhyay, U, Bhattacharyya, D K, Banerjee, R K

The mechanism of inhibition of gastric peroxidase (GPO) activity by mercaptomethylimidazole (MMI), an inducer of gastric acid secretion, has been investigated. Incubation of purified GPO with MMI in...

Mechanism of inhibition of horseradish peroxidase-catalysed iodide oxidation by EDTA.

Bhattacharyya, D K, Adak, S, Bandyopadhyay, U, Banerjee, R K

EDTA inhibits horseradish peroxidase (HRP)-catalysed iodide oxidation in a concentration and pH-dependent manner. It is more effective at pH 6 than at lower pH values. A plot of log Kiapp. values as...

Characterization of sheep lacrimal-gland peroxidase and its major physiological electron donor.

Mazumdar, A, Chatterjee, R, Adak, S, Ghosh, A, Mondal, C, Banerjee, R K

A soluble sheep lacrimal-gland peroxidase was purified to apparent homogeneity. It had a native molecular mass of 75 kDa with a subunit molecular mass of 82 kDa and an isoelectric point of 6.5....

Probing the active site residues in aromatic donor oxidation in horseradish peroxidase: involvement of an arginine and a tyrosine residue in aromatic donor binding.

Adak, S, Mazumder, A, Banerjee, R K

The plausible role of arginine and tyrosine residues at the active side of horseradish peroxidase (HRP) in aromatic donor (guaiacol) oxidation was probed by chemical modification followed by...

Mechanism-based inactivation of lacrimal-gland peroxidase by phenylhydrazine: a suicidal substrate to probe the active site.

Mazumdar, A, Adak, S, Chatterjee, R, Banerjee, R K

Humans are exposed to various hydrazine derivatives for therapeutic control of several diseases, and mammalian peroxidases are implicated in the oxidative metabolism of many drugs. The results...

Haem propionates control oxidative and reductive activities of horseradish peroxidase by maintaining the correct orientation of the haem.

Adak, S, Banerjee, R K

The role of haem propionates in oxidative and reductive reactions catalysed by horseradish peroxidase (HRP) was studied after successful reconstitution of ferric protoporphyrin IX dimethyl ester...